##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431350_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2847400
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63826929830723	33.0	31.0	34.0	26.0	34.0
2	30.80150242326333	34.0	31.0	34.0	26.0	34.0
3	30.921140689752054	34.0	31.0	34.0	26.0	34.0
4	33.98742677530379	37.0	35.0	37.0	28.0	37.0
5	34.275225468848774	37.0	35.0	37.0	31.0	37.0
6	34.24341574769966	37.0	35.0	37.0	30.0	37.0
7	34.211306103814	37.0	35.0	37.0	30.0	37.0
8	34.144953641918946	37.0	35.0	37.0	30.0	37.0
9	35.53618880382103	39.0	37.0	39.0	28.0	39.0
10-11	35.59623516190209	39.0	37.0	39.0	30.0	39.0
12-13	35.5471345789141	39.0	37.0	39.0	29.5	39.0
14-15	36.8836612348107	41.0	38.0	41.0	29.5	41.0
16-17	36.88720007726347	41.0	38.0	41.0	30.0	41.0
18-19	36.87169786471869	41.0	38.0	41.0	30.0	41.0
20-21	36.89450165062864	41.0	38.0	41.0	29.0	41.0
22-23	36.90911954765751	40.5	38.0	41.0	29.0	41.0
24-25	36.93705678864929	41.0	38.0	41.0	29.0	41.0
26-27	36.94682903701623	40.0	38.0	41.0	29.5	41.0
28-29	36.92528376764768	40.0	38.0	41.0	30.0	41.0
30-31	36.846144201727896	40.0	38.0	41.0	30.0	41.0
32-33	36.76391234108309	40.0	37.0	41.0	29.5	41.0
34-35	36.698665097984126	40.0	37.0	41.0	29.0	41.0
36-37	36.60096948092997	40.0	37.0	41.0	29.0	41.0
38-39	36.49486461333146	40.0	37.0	41.0	28.0	41.0
40-41	36.405972641708225	40.0	37.0	41.0	27.5	41.0
42-43	36.274468638055765	40.0	36.5	41.0	27.0	41.0
44-45	36.144932570063915	40.0	36.0	41.0	27.0	41.0
46-47	36.01636949497787	40.0	36.0	41.0	27.0	41.0
48-49	35.869734845824254	40.0	35.0	41.0	27.0	41.0
50-51	35.728792933904614	40.0	35.0	41.0	27.0	41.0
52-53	35.5579951885931	40.0	35.0	41.0	27.0	41.0
54-55	35.391950551380205	39.0	35.0	41.0	27.0	41.0
56-57	35.189636686099604	39.0	35.0	41.0	27.0	41.0
58-59	34.953408548149184	39.0	34.5	41.0	26.0	41.0
60-61	34.75851021984968	38.0	34.0	41.0	26.0	41.0
62-63	34.57626747207979	38.0	34.0	40.0	26.0	41.0
64-65	34.31835411252371	37.5	34.0	40.0	26.0	41.0
66-67	33.99811336658004	37.0	34.0	40.0	25.5	41.0
68-69	33.67529061600056	36.5	33.5	39.5	25.0	41.0
70-71	33.41578457540212	36.0	33.0	39.0	25.0	41.0
72-73	33.154901313478966	35.5	33.0	39.0	25.0	41.0
74-75	32.81789316569502	35.0	33.0	38.5	24.5	40.5
76-77	32.48704467233266	35.0	33.0	37.0	24.0	39.5
78-79	32.16563443843506	35.0	33.0	37.0	23.0	39.0
80-81	31.846225679567326	35.0	33.0	36.5	22.5	39.0
82-83	31.536106974784012	35.0	32.0	36.0	20.5	38.0
84-85	31.271815515909253	35.0	32.0	36.0	20.0	37.0
86-87	31.03664746786542	35.0	32.0	35.0	19.5	37.0
88-89	30.811826227435553	35.0	32.0	35.0	18.0	36.0
90-91	30.62298834024022	35.0	32.0	35.0	17.5	36.0
92-93	30.439683219779447	35.0	31.5	35.0	11.0	36.0
94-95	30.240492730210015	35.0	31.0	35.0	4.5	36.0
96-97	30.06603269649505	35.0	31.0	35.0	2.0	35.0
98-99	29.95168996277306	35.0	31.0	35.0	2.0	35.0
100	29.911957575331883	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2714195234842265
1101	2	0.3993142787174513
1101	3	0.38803803995400443
1101	4	0.45106852874027936
1101	5	0.35541838278842874
1101	6	0.3223739528400884
1101	7	0.37876053790024145
1101	8	0.4021285043600926
1101	9	0.47901874333535943
1101	10-11	0.4170067657420802
1101	12-13	0.429303491716837
1101	14-15	0.484965249823631
1101	16-17	0.5474743243682809
1101	18-19	0.43298951588821666
1101	20-21	0.3599775723888001
1101	22-23	0.29830840936815406
1101	24-25	0.3244816322231543
1101	26-27	0.3988327867648209
1101	28-29	0.3697956806667335
1101	30-31	0.464818275815702
1101	32-33	0.4362167311086864
1101	34-35	0.517209905086844
1101	36-37	0.5527915569421324
1101	38-39	0.5322242459343727
1101	40-41	0.47183617683286627
1101	42-43	0.516164517342439
1101	44-45	0.48122708131779035
1101	46-47	0.5623810326804985
1101	48-49	0.5738596209472959
1101	50-51	0.5868060270620248
1101	52-53	0.5330931084656498
1101	54-55	0.5070309244120565
1101	56-57	0.5415713906415078
1101	58-59	0.5539918985489081
1101	60-61	0.5726058661404139
1101	62-63	0.6001172608224863
1101	64-65	0.6422675634875574
1101	66-67	0.6131101050514829
1101	68-69	0.562060210852259
1101	70-71	0.551148685286492
1101	72-73	0.5615874429334582
1101	74-75	0.6056688487904509
1101	76-77	0.6364972333309211
1101	78-79	0.6255462156008988
1101	80-81	0.5403067690006829
1101	82-83	0.5717185910932088
1101	84-85	0.5873834897768688
1101	86-87	0.5508526780526886
1101	88-89	0.5214771898607928
1101	90-91	0.5110088498208576
1101	92-93	0.5027834649399416
1101	94-95	0.5687226513632488
1101	96-97	0.5839270398657881
1101	98-99	0.6254473584291809
1101	100	0.5904379894331093
1102	1	0.257183685523529
1102	2	0.36506286104010854
1102	3	0.42080835956881657
1102	4	0.36751807018924154
1102	5	0.2773956487019049
1102	6	0.41574796085031807
1102	7	0.2789902618471345
1102	8	0.21983966973827052
1102	9	0.3938971164849505
1102	10-11	0.3460505734453321
1102	12-13	0.3131083378287016
1102	14-15	0.396537161234491
1102	16-17	0.4053413765987628
1102	18-19	0.3528617903987268
1102	20-21	0.34686889361539386
1102	22-23	0.2785454875562934
1102	24-25	0.3515146186857123
1102	26-27	0.37242427019471336
1102	28-29	0.4556909121972055
1102	30-31	0.4503447703025856
1102	32-33	0.4124540117306452
1102	34-35	0.4371140180807913
1102	36-37	0.4126506604421891
1102	38-39	0.4380169280799677
1102	40-41	0.4199137602343157
1102	42-43	0.3779331611127361
1102	44-45	0.4286846791202379
1102	46-47	0.36082805780022653
1102	48-49	0.3343739437805553
1102	50-51	0.34245630086017087
1102	52-53	0.2638561942745312
1102	54-55	0.3107699282839249
1102	56-57	0.2918305828024472
1102	58-59	0.3762518030718951
1102	60-61	0.4189745493724928
1102	62-63	0.41016130939635076
1102	64-65	0.4363914094226047
1102	66-67	0.4326214580012575
1102	68-69	0.40122897659532697
1102	70-71	0.4340166343756735
1102	72-73	0.4623151796977467
1102	74-75	0.42630018605800757
1102	76-77	0.38856494103698935
1102	78-79	0.44448095247537367
1102	80-81	0.47040227163872217
1102	82-83	0.4491322300076561
1102	84-85	0.4396586921466259
1102	86-87	0.4487204605823898
1102	88-89	0.5090722889124315
1102	90-91	0.43780423279233105
1102	92-93	0.42126759438483674
1102	94-95	0.38103946797614796
1102	96-97	0.40906502798112854
1102	98-99	0.3650509084521154
1102	100	0.34335777695447334
1103	1	0.3254769703139395
1103	2	0.3303423972755155
1103	3	0.3434169468318693
1103	4	0.4330284257382999
1103	5	0.3185143884082038
1103	6	0.33223028210296945
1103	7	0.3906240487834438
1103	8	0.36923114321714223
1103	9	0.5160716520781321
1103	10-11	0.4153270232316899
1103	12-13	0.34292316354050456
1103	14-15	0.39929240860914206
1103	16-17	0.4033117737686993
1103	18-19	0.4066754025374948
1103	20-21	0.399355199791394
1103	22-23	0.4208775788438075
1103	24-25	0.3662373855816199
1103	26-27	0.36278822821444834
1103	28-29	0.3747911154928403
1103	30-31	0.3505978932916918
1103	32-33	0.322577855656796
1103	34-35	0.33955979800393266
1103	36-37	0.3588730189119289
1103	38-39	0.378813640676384
1103	40-41	0.44529584908583075
1103	42-43	0.3657418139264479
1103	44-45	0.37965531609422243
1103	46-47	0.29275755667302406
1103	48-49	0.25323700047871256
1103	50-51	0.19037130266028157
1103	52-53	0.21948070667822606
1103	54-55	0.2236377570195387
1103	56-57	0.15593647116259035
1103	58-59	0.19483339054944082
1103	60-61	0.22290597553593727
1103	62-63	0.2669516238433758
1103	64-65	0.2688352848333224
1103	66-67	0.25804885020490076
1103	68-69	0.2527786233114355
1103	70-71	0.22466566816780187
1103	72-73	0.18803720187234774
1103	74-75	0.23757113490381698
1103	76-77	0.1951953333855343
1103	78-79	0.15361622157791288
1103	80-81	0.1557265797344236
1103	82-83	0.22820714314986645
1103	84-85	0.2282638234472536
1103	86-87	0.26367423661046274
1103	88-89	0.26532873878737107
1103	90-91	0.17280372977326763
1103	92-93	0.17705677434987166
1103	94-95	0.1878509471875418
1103	96-97	0.1453080260506745
1103	98-99	0.24090419012950193
1103	100	0.20393424312850073
1104	1	0.20203922075590697
1104	2	0.17834272219835867
1104	3	0.21724644247148106
1104	4	0.18860686249717418
1104	5	0.08792692753935683
1104	6	0.13015132475403135
1104	7	0.10548614044132876
1104	8	0.14800826608755813
1104	9	0.17081002789933564
1104	10-11	0.2487993086486
1104	12-13	0.20049782673035565
1104	14-15	0.19907809857530623
1104	16-17	0.23248614957746128
1104	18-19	0.19760712794182922
1104	20-21	0.15778393804610147
1104	22-23	0.17908366445110602
1104	24-25	0.3074745585845591
1104	26-27	0.3065587382829378
1104	28-29	0.3803581634864628
1104	30-31	0.3955597322378992
1104	32-33	0.3911834115813093
1104	34-35	0.43037939209553855
1104	36-37	0.36762142013967747
1104	38-39	0.36596601576907517
1104	40-41	0.39319430630605723
1104	42-43	0.3714878805030608
1104	44-45	0.35522911318906125
1104	46-47	0.37143718439486406
1104	48-49	0.38609833581281094
1104	50-51	0.3137179420963605
1104	52-53	0.24733268675913678
1104	54-55	0.20949638667573112
1104	56-57	0.2374666748083456
1104	58-59	0.1940522767629531
1104	60-61	0.23367361856281832
1104	62-63	0.2360668475946497
1104	64-65	0.3392341367726104
1104	66-67	0.31830511480333
1104	68-69	0.3074474407624379
1104	70-71	0.2562593836184419
1104	72-73	0.26652198565869867
1104	74-75	0.3161184882919841
1104	76-77	0.3087384871093448
1104	78-79	0.29531761711787397
1104	80-81	0.328270123694967
1104	82-83	0.33016220536911334
1104	84-85	0.2810724759084273
1104	86-87	0.28064825357707335
1104	88-89	0.30658232258938156
1104	90-91	0.26298061330581035
1104	92-93	0.29309685768087235
1104	94-95	0.34584907119720043
1104	96-97	0.2836158439760581
1104	98-99	0.2740097298627546
1104	100	0.34062723476594314
1105	1	0.10547283589676226
1105	2	0.16329781020561285
1105	3	0.19330236468218587
1105	4	0.10243145848593826
1105	5	0.1812151804110229
1105	6	0.13674565359844593
1105	7	0.23254703895072737
1105	8	0.13500246547499017
1105	9	0.19009452855836884
1105	10-11	0.1903274802035071
1105	12-13	0.2221217687683037
1105	14-15	0.2673023651299218
1105	16-17	0.272698770103311
1105	18-19	0.22463036226461242
1105	20-21	0.22482682768329454
1105	22-23	0.22809191068600398
1105	24-25	0.27963821129671373
1105	26-27	0.2641023849375017
1105	28-29	0.2435060334838255
1105	30-31	0.26880672206256406
1105	32-33	0.2902845524892541
1105	34-35	0.2752204275546859
1105	36-37	0.3318673978759179
1105	38-39	0.3256942945248511
1105	40-41	0.34954018447261603
1105	42-43	0.35558714639746825
1105	44-45	0.31374933319307985
1105	46-47	0.3096068142021693
1105	48-49	0.25370198107955133
1105	50-51	0.24067947126233946
1105	52-53	0.2710715056590516
1105	54-55	0.23931292675394644
1105	56-57	0.254449890022201
1105	58-59	0.2645176352730161
1105	60-61	0.2914672002430194
1105	62-63	0.3363152345275111
1105	64-65	0.38712484215106713
1105	66-67	0.36857742432281526
1105	68-69	0.34319299404806003
1105	70-71	0.374038919543942
1105	72-73	0.34511273676963583
1105	74-75	0.38729997630783686
1105	76-77	0.397480173623471
1105	78-79	0.3892730069121555
1105	80-81	0.381300588157405
1105	82-83	0.48063828032375966
1105	84-85	0.49398304149592676
1105	86-87	0.46823894221791207
1105	88-89	0.4297695287135639
1105	90-91	0.4050336975892641
1105	92-93	0.3714848086380229
1105	94-95	0.382121067091294
1105	96-97	0.41680600604680507
1105	98-99	0.4078544619853339
1105	100	0.3886121006109562
1106	1	0.062217467936324766
1106	2	0.13831436897430294
1106	3	-0.019330439536567212
1106	4	0.0017398458278350404
1106	5	-0.009541494462254718
1106	6	-0.017043985097821235
1106	7	0.009665927250068762
1106	8	0.004787134242590696
1106	9	0.10435100281988952
1106	10-11	0.09792325252984568
1106	12-13	0.0716487352614621
1106	14-15	0.0035733968088820234
1106	16-17	0.15557563295414667
1106	18-19	0.1505778123674375
1106	20-21	0.11059745345750116
1106	22-23	0.047347439340121866
1106	24-25	0.10528761207441306
1106	26-27	0.1343052998595624
1106	28-29	0.13796016097077057
1106	30-31	0.0714673089247313
1106	32-33	0.08655143745420446
1106	34-35	0.10956107534427417
1106	36-37	0.06527351999869069
1106	38-39	0.08627730957746138
1106	40-41	0.07612482586782221
1106	42-43	0.12451916837505195
1106	44-45	0.08806467853724342
1106	46-47	0.11160581633528466
1106	48-49	0.05332938963352518
1106	50-51	0.12342840900733165
1106	52-53	-0.012716255440516022
1106	54-55	0.011636793969891812
1106	56-57	0.04738314716221481
1106	58-59	0.0870410843790026
1106	60-61	0.08532333956715377
1106	62-63	0.07497224391259749
1106	64-65	0.15336260649344524
1106	66-67	0.1595137560912505
1106	68-69	0.12485803249963823
1106	70-71	0.0767493146376097
1106	72-73	0.057812525059119935
1106	74-75	0.04067681609300422
1106	76-77	0.07213598870045956
1106	78-79	0.08416575664826809
1106	80-81	0.028391479328117697
1106	82-83	0.014230919463805947
1106	84-85	0.042944396879018853
1106	86-87	0.06666586344788072
1106	88-89	0.14971789565553806
1106	90-91	0.06818399843881195
1106	92-93	0.09860259897737222
1106	94-95	0.06519917153912047
1106	96-97	0.08102771157529531
1106	98-99	0.07684880383151338
1106	100	0.0883151066892296
1107	1	-0.05735026265439913
1107	2	0.06507935039878632
1107	3	0.02300826745584672
1107	4	0.015650508169350985
1107	5	-0.0083194492960601
1107	6	0.007630078993734912
1107	7	-0.04033071773449137
1107	8	-0.033358981545909216
1107	9	-0.004351599903770875
1107	10-11	-0.005730845890433045
1107	12-13	0.04107258435030303
1107	14-15	0.1286646865229173
1107	16-17	0.0492007962870602
1107	18-19	0.04716130538414376
1107	20-21	0.008464892955821313
1107	22-23	0.0856882228565965
1107	24-25	0.09695303661703747
1107	26-27	0.053470889591885395
1107	28-29	0.04298699853321608
1107	30-31	0.058646309498925575
1107	32-33	0.11424737163648047
1107	34-35	0.18408424057341222
1107	36-37	0.10697974255351284
1107	38-39	0.0889278229972632
1107	40-41	0.0609193836742179
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2216	66-67	-0.9834976735432761
2216	68-69	-1.012822326344299
2216	70-71	-0.9549189317043059
2216	72-73	-0.9405629548627488
2216	74-75	-0.9682403085284434
2216	76-77	-0.9004543579734019
2216	78-79	-0.8983534220130096
2216	80-81	-0.8728352833202528
2216	82-83	-0.8493772942433324
2216	84-85	-0.8131116942083594
2216	86-87	-0.8528624764413699
2216	88-89	-0.9253606349941599
2216	90-91	-0.8521411090333011
2216	92-93	-0.927958689668035
2216	94-95	-0.9130718090447338
2216	96-97	-0.9804651010800178
2216	98-99	-1.0485527339796725
2216	100	-0.9871097664258883
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61737.0
3	15841.0
4	13097.0
5	12757.0
6	7342.0
7	5486.0
8	5762.0
9	6003.0
10	5654.0
11	6202.0
12	7096.0
13	7673.0
14	7768.0
15	7679.0
16	7689.0
17	7795.0
18	7652.0
19	7484.0
20	7061.0
21	7561.0
22	9625.0
23	18661.0
24	33788.0
25	39726.0
26	39687.0
27	26440.0
28	25422.0
29	27956.0
30	32347.0
31	38410.0
32	51104.0
33	67791.0
34	97797.0
35	170686.0
36	286316.0
37	555415.0
38	785442.0
39	324006.0
40	1442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50197988680842	22.67891416159037	12.912654944800227	21.906451006800978
2	15.565695814702277	23.930426256130165	38.66722436921313	21.836653559954428
3	17.065012665945307	31.20416211426639	29.059046519297084	22.67177870049122
4	11.763336210814224	21.0331327057194	37.98752404827144	29.21600703519494
5	11.652463055744226	42.036975512380074	33.53328196479733	12.777279467078365
6	26.687890707311933	41.97502985179462	18.370443211350707	12.966636229542742
7	24.484722905106413	36.759499894640726	22.70025286226031	16.055524337992555
8	23.430954555032663	38.83226803399592	20.2420804944862	17.494696916485214
9	24.94524829669172	20.573294935730843	20.563812600969307	33.917644166608135
10-11	22.341100653227507	30.768701271335253	27.918662639601038	18.971535435836202
12-13	22.959436679075647	29.701359134649152	27.0359450726979	20.303259113577298
14-15	21.196969164852145	30.38914448268596	25.39885509587694	23.015031256584955
16-17	20.56663974151858	33.00712931095034	25.562126852567253	20.864104094963825
18-19	20.595209664957505	32.04103743766243	27.510377888600125	19.85337500877994
20-21	22.41083093348318	30.719516049729577	26.95522230807052	19.914430708716722
22-23	21.875447776919295	30.953115122567958	26.794373814708155	20.377063285804596
24-25	21.24194001545269	31.343576596193017	26.65154175739271	20.762941630961578
26-27	20.986584252300343	31.297376554049304	26.79699023670717	20.919048956943175
28-29	21.310686942473836	30.911463089133946	27.20522230807052	20.572627660321697
30-31	21.463141813584322	31.19363278780642	26.86257638547447	20.480649013134787
32-33	20.72304558544637	31.008744819835638	27.242818009412094	21.025391585305893
34-35	21.341258692140197	30.689471096438858	27.20941911919646	20.759851092224483
36-37	21.754951885931025	30.789632647327387	26.587413078598022	20.86800238814357
38-39	21.351601460981946	30.83607852777973	26.911831846596897	20.900488164641427
40-41	21.008867738989956	30.81160005619161	27.18922525813022	20.990306946688207
42-43	21.0218620495891	30.83153051907003	27.218128819273723	20.92847861206715
44-45	20.98296691718761	30.789246329985247	27.09972255390883	21.128064198918313
46-47	21.359995083233827	30.766137528973804	26.878608555173138	20.995258832619232
48-49	21.15055840415818	30.476785839713422	27.256918592400087	21.115737163728312
50-51	21.111628151998314	30.654456697337924	26.836148767296482	21.397766383367284
52-53	21.013872304558546	30.743432605183674	26.904579616492242	21.33811547376554
54-55	21.04512888951324	31.141216548430144	26.912815199831424	20.900839362225188
56-57	21.105095876940368	30.907266278008006	26.687100512748472	21.300537332303154
58-59	21.297762871391445	30.10207557772003	27.039720446723326	21.560441104165204
60-61	21.470622322118423	30.592768841750367	26.864701130856222	21.071907705274988
62-63	20.914711666783735	30.724239657231156	26.990447425721708	21.370601250263398
64-65	21.01597949006111	30.648697056964245	27.08855447074524	21.2467689822294
66-67	21.0491501018473	30.27388143569572	26.908741307859803	21.768227154597174
68-69	21.382313689681816	30.67257849265997	26.812478050151018	21.1326297675072
70-71	21.45771581091522	30.356008990658147	27.028622603076492	21.157652595350143
72-73	21.131804453185364	30.279184519210506	27.097843646835713	21.49116738076842
74-75	20.964985600899066	30.424492519491466	27.23705836903842	21.373463510571046
76-77	21.07768490552785	30.6066587061881	26.868283346210582	21.44737304207347
78-79	21.29029992273653	30.598229964177847	26.909689541335958	21.20178057174967
80-81	20.991834656177566	30.68783802767437	27.066481702605888	21.25384561354218
82-83	20.839668061975157	30.722968575882764	27.063997704571786	21.373365657570297
84-85	21.00170665634607	30.3456106410787	26.966241427866773	21.686441274708457
86-87	21.243853955945156	30.548631012587098	26.86688722184761	21.340627809620138
88-89	20.951340701138534	30.54162763111336	27.030438512308603	21.4765931554395
90-91	20.83806074607573	30.510096980959055	26.93835922061917	21.713483052346046
92-93	20.87720777008636	30.608289169151448	27.16767989660489	21.346823164157296
94-95	21.046945754097816	30.235523371905597	27.11784105829319	21.599689815703396
96-97	20.98862533858385	30.710865852748405	26.82239109343378	21.478117715233967
98-99	20.51616959105404	30.550658162763582	27.10893050306955	21.82424174311282
100	20.691474192465304	30.544711004218723	27.08143973184496	21.682375071471014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50705.0
1	33497.5
2	12768.0
3	7011.5
4	3748.5
5	2398.5
6	2100.0
7	2465.0
8	3090.5
9	3164.0
10	2288.5
11	1489.0
12	1319.5
13	1285.5
14	1310.0
15	1411.0
16	1551.5
17	1780.0
18	2169.0
19	2634.5
20	3449.0
21	4567.5
22	6023.5
23	8231.0
24	11408.5
25	15035.5
26	19390.5
27	24868.0
28	30907.5
29	37299.0
30	43148.0
31	48681.0
32	53875.0
33	57843.0
34	62504.0
35	70514.5
36	78217.0
37	85198.5
38	94580.5
39	100415.5
40	104631.5
41	108484.0
42	109744.0
43	113825.0
44	114130.5
45	108952.0
46	106140.0
47	104193.0
48	97883.0
49	93912.0
50	93874.5
51	87447.0
52	76793.5
53	68639.0
54	63071.5
55	58755.5
56	53066.5
57	46136.0
58	42093.0
59	39831.5
60	36019.0
61	30776.5
62	25807.0
63	20624.5
64	16005.0
65	12764.0
66	10256.0
67	8159.5
68	6222.0
69	4671.0
70	3539.0
71	2735.5
72	2165.0
73	1654.0
74	1269.5
75	998.5
76	726.0
77	532.5
78	408.0
79	291.5
80	209.0
81	158.0
82	130.0
83	100.0
84	77.5
85	68.5
86	66.0
87	55.5
88	46.5
89	36.5
90	30.0
91	28.0
92	23.0
93	16.5
94	15.5
95	15.0
96	15.5
97	13.5
98	11.0
99	12.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.267963756409356E-4
2	1.404790335042495E-4
3	3.5119758376062375E-5
4	7.023951675212475E-5
5	7.023951675212475E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.5119758376062375E-5
84-85	6.848352883332163E-4
86-87	0.0014047903350424948
88-89	0.0035997752335463935
90-91	0.00310809861628152
92-93	0.0024583830863243662
94-95	0.002124745381751774
96-97	0.0021949848985038985
98-99	0.0028095806700849896
100	0.003231017770597738
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2847400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.081642345157945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3320147978227	41.04910351328486
2	13.302364884413134	14.122227502784124
3	4.157724802387336	6.620965827897511
4	1.784057200567098	3.7880274497522564
5	0.8974320085958932	2.3818582454691954
6	0.5717979751263519	1.8211185365605516
7	0.35454117850782696	1.317373962392829
8	0.23941215275868527	1.016671221265668
9	0.18873386820399413	0.901647332138033
>10	0.9955437811109272	9.829633041155624
>50	0.09715435817176324	3.603283005282031
>100	0.07002686078662924	7.123354598007263
>500	0.006483603537290666	2.3591839031231494
>1k	0.002646368790703432	2.0713183748993464
>5k	0.0	0.0
>10k+	6.61592197675858E-5	1.994233485987594
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56786	1.994310599143078	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4354	0.15291142796937557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.5119758376062375E-5	0.0
12-13	0.0	0.0	0.0	3.5119758376062375E-5	0.0
14-15	0.0	0.0	0.0	3.5119758376062375E-5	0.0
16-17	0.0	0.0	0.0	7.023951675212475E-5	0.0
18-19	0.0	0.0	0.0	1.0535927512818712E-4	0.0
20-21	0.0	0.0	0.0	2.1071855025637425E-4	0.0
22-23	0.0	0.0	0.0	8.955538385895906E-4	0.0
24-25	0.0	0.0	0.0	0.0017384280396150874	0.0
26-27	0.0	0.0	0.0	0.0025813022406405844	0.0
28-29	0.0	0.0	0.0	0.005215284118845262	0.0
30-31	0.0	0.0	0.0	0.01048324787525462	0.0
32-33	0.0	0.0	0.0	0.019719744328159025	0.0
34-35	0.0	0.0	0.0	0.03211701903490904	0.0
36-37	0.0	0.0	0.0	0.05069537121584604	0.0
38-39	0.0	0.0	0.0	0.07786050431973028	0.0
40-41	0.0	0.0	0.0	0.10655334691297325	0.0
42-43	0.0	0.0	0.0	0.13463159373463512	0.0
44-45	0.0	0.0	0.0	0.1669593313198005	0.0
46-47	0.0	0.0	0.0	0.20682025707663132	0.0
48-49	0.0	0.0	0.0	0.2491571257989745	0.0
50-51	0.0	0.0	0.0	0.29614736250614593	0.0
52-53	0.0	0.0	0.0	0.34150453044883056	0.0
54-55	0.0	0.0	0.0	0.38977663833672826	0.0
56-57	0.0	0.0	0.0	0.4480754372409918	0.0
58-59	0.0	0.0	0.0	0.5154000140479034	0.0
60-61	0.0	0.0	0.0	0.591223572381822	0.0
62-63	0.0	0.0	0.0	0.667187609749245	0.0
64-65	0.0	0.0	0.0	0.7496488024162393	0.0
66-67	0.0	0.0	0.0	0.8475802486478893	0.0
68-69	0.0	0.0	0.0	0.961175107115263	0.0
70-71	0.0	0.0	0.0	1.0893095455503266	0.0
72-73	0.0	0.0	0.0	1.2259078457540213	0.0
74-75	0.0	0.0	0.0	1.3696881365456206	0.0
76-77	0.0	0.0	0.0	1.5152595350143991	0.0
78-79	0.0	0.0	0.0	1.683500737514926	0.0
80-81	0.0	0.0	0.0	1.8770984055629696	0.0
82-83	0.0	0.0	0.0	2.086166327175669	0.0
84-85	0.0	0.0	0.0	2.3039263889864436	0.0
86-87	0.0	0.0	0.0	2.52907915993538	0.0
88	0.0	0.0	0.0	2.715178759570134	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5035	0.0	39.298553	1
GTATCAA	8365	0.0	34.77913	1
ATCAACG	9660	0.0	28.60844	3
TCAACGC	9720	0.0	28.383492	4
TACCTGG	2245	0.0	28.262552	2
CAACGCA	10315	0.0	26.746246	5
GTACCTG	2485	0.0	26.478638	1
AACGCAG	10740	0.0	25.68785	6
TACATGG	15875	0.0	25.194736	2
GTACACG	300	0.0	25.066442	1
GTACATG	17020	0.0	24.963346	1
ACGCAGA	11690	0.0	23.680712	7
CGCAGAG	11755	0.0	23.509785	8
ACCTGGG	2640	0.0	23.49979	3
TATAACG	180	2.163415E-7	23.499788	2
TACACGG	240	2.6193447E-10	23.499788	2
TATCAAC	12185	0.0	23.374432	2
ACATGGG	16700	0.0	22.739916	3
GCAGAGT	12705	0.0	20.827047	9
CATGGGT	3330	0.0	20.747562	4
>>END_MODULE