##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431350_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2847400
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72712544777692	34.0	31.0	34.0	31.0	34.0
2	32.83389126922807	34.0	31.0	34.0	31.0	34.0
3	32.908130575261644	34.0	31.0	34.0	31.0	34.0
4	36.31070625834094	37.0	37.0	37.0	35.0	37.0
5	36.282193228910586	37.0	37.0	37.0	35.0	37.0
6	36.32537367422912	37.0	37.0	37.0	35.0	37.0
7	36.19017770597738	37.0	37.0	37.0	35.0	37.0
8	36.24300519772424	37.0	37.0	37.0	35.0	37.0
9	38.04927934255812	39.0	39.0	39.0	35.0	39.0
10-11	38.03926564585235	39.0	39.0	39.0	35.0	39.0
12-13	38.02073172016577	39.0	39.0	39.0	35.0	39.0
14-15	39.45748823488094	41.0	39.0	41.0	36.0	41.0
16-17	39.47933535857273	41.0	39.0	41.0	36.5	41.0
18-19	39.565924527639254	41.0	40.0	41.0	37.0	41.0
20-21	39.540154702535645	41.0	40.0	41.0	37.0	41.0
22-23	39.42630171384421	41.0	39.0	41.0	36.0	41.0
24-25	39.379574699726064	41.0	39.0	41.0	36.0	41.0
26-27	39.268412938118985	41.0	39.0	41.0	35.0	41.0
28-29	39.16551239727471	41.0	39.0	41.0	35.0	41.0
30-31	38.925648837536	41.0	38.5	41.0	35.0	41.0
32-33	38.64232106483107	41.0	38.0	41.0	35.0	41.0
34-35	38.49367264873217	41.0	38.0	41.0	35.0	41.0
36-37	38.35733458593805	41.0	38.0	41.0	35.0	41.0
38-39	38.22836675563673	40.0	38.0	41.0	35.0	41.0
40-41	38.1148117580951	40.0	38.0	41.0	34.5	41.0
42-43	37.945042670506425	40.0	38.0	41.0	34.0	41.0
44-45	37.68748033293531	40.0	37.5	41.0	33.0	41.0
46-47	37.29711210226874	40.0	37.0	41.0	33.0	41.0
48-49	36.949310774741875	40.0	37.0	41.0	31.5	41.0
50-51	36.762125974573294	40.0	37.0	41.0	31.0	41.0
52-53	36.64065990025989	40.0	36.0	41.0	31.0	41.0
54-55	36.48046077122989	40.0	36.0	41.0	31.0	41.0
56-57	36.29795971763714	40.0	35.0	41.0	31.0	41.0
58-59	36.09605130996699	39.5	35.0	41.0	30.5	41.0
60-61	35.88174580318888	39.0	35.0	41.0	30.0	41.0
62-63	35.63005478682307	39.0	35.0	41.0	30.0	41.0
64-65	35.35091328931657	38.5	35.0	41.0	29.0	41.0
66-67	35.04348686521037	37.0	35.0	40.5	29.0	41.0
68-69	34.75746488024163	37.0	35.0	40.0	29.0	41.0
70-71	34.48566341223572	36.5	35.0	39.5	29.0	41.0
72-73	34.214984371707516	36.0	35.0	39.0	29.0	41.0
74-75	33.91443667907565	35.5	35.0	39.0	29.0	41.0
76-77	33.607373568869846	35.0	35.0	37.5	28.5	40.0
78-79	33.30180726276603	35.0	34.5	37.0	28.0	39.0
80-81	33.00872690875887	35.0	34.0	37.0	27.5	39.0
82-83	32.71566411463089	35.0	34.0	36.0	27.0	38.5
84-85	32.47938048746225	35.0	34.0	36.0	27.0	37.0
86-87	32.27994029641076	35.0	34.0	35.5	26.0	37.0
88-89	32.07439576455714	35.0	34.0	35.0	26.0	36.5
90-91	31.894709032801856	35.0	34.0	35.0	25.5	36.0
92-93	31.756835885369107	35.0	34.0	35.0	25.0	36.0
94-95	31.627854182763222	35.0	34.0	35.0	25.0	36.0
96-97	31.493331460279553	35.0	34.0	35.0	24.5	36.0
98-99	31.376015487813444	35.0	34.0	35.0	24.0	35.5
100	31.27418943597668	35.0	34.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22203533811260456
1101	2	0.21282522873959664
1101	3	0.21889217461688304
1101	4	0.141118837515954
1101	5	0.17486640611801363
1101	6	0.20386833212809563
1101	7	0.2212584807343987
1101	8	0.21662467771559335
1101	9	0.28143133821124877
1101	10-11	0.27091562089741217
1101	12-13	0.26312028888781214
1101	14-15	0.3200328121187326
1101	16-17	0.3263042017808644
1101	18-19	0.3528188046134275
1101	20-21	0.36689621009160334
1101	22-23	0.39137851940820667
1101	24-25	0.3654298345778244
1101	26-27	0.33866417764564005
1101	28-29	0.36638263064439514
1101	30-31	0.41119745908479644
1101	32-33	0.4213288624451934
1101	34-35	0.43833090678602815
1101	36-37	0.4118236035692462
1101	38-39	0.44978103372036315
1101	40-41	0.44983734211323423
1101	42-43	0.4703899310915318
1101	44-45	0.5180283887394026
1101	46-47	0.5794005972988359
1101	48-49	0.5782068623783445
1101	50-51	0.534566080639749
1101	52-53	0.5321398908360564
1101	54-55	0.5470254013764233
1101	56-57	0.6123579891772906
1101	58-59	0.64185695933989
1101	60-61	0.5767589334421501
1101	62-63	0.5502127464146511
1101	64-65	0.5720749108922618
1101	66-67	0.5066521958524177
1101	68-69	0.4555956846463687
1101	70-71	0.405479628236769
1101	72-73	0.36070329909676246
1101	74-75	0.34283489604734285
1101	76-77	0.41335136400063277
1101	78-79	0.3791408034942876
1101	80-81	0.34479061439948566
1101	82-83	0.3797189112706292
1101	84-85	0.30358738656527606
1101	86-87	0.24162924540291897
1101	88-89	0.28358991084445506
1101	90-91	0.2419430759728023
1101	92-93	0.27361259625787326
1101	94-95	0.21999306406312513
1101	96-97	0.22147903471523378
1101	98-99	0.23679282581241523
1101	100	0.3010005618194178
1102	1	0.15237529880457146
1102	2	0.2180368745777912
1102	3	0.1709708368112146
1102	4	0.084060559638381
1102	5	0.1074344200372579
1102	6	0.11381603245430227
1102	7	0.17917005559262833
1102	8	0.16122981645963108
1102	9	0.1795370445387121
1102	10-11	0.2581804197063775
1102	12-13	0.22368976853039868
1102	14-15	0.29825522538609306
1102	16-17	0.27568767549393414
1102	18-19	0.2848526751224085
1102	20-21	0.2314219896539882
1102	22-23	0.22362680320505035
1102	24-25	0.20823818100102898
1102	26-27	0.2394633427983166
1102	28-29	0.22178229650047854
1102	30-31	0.23623461465903262
1102	32-33	0.27472917403953545
1102	34-35	0.21099862576689077
1102	36-37	0.1767026173321824
1102	38-39	0.15826691844583252
1102	40-41	0.1925092378931268
1102	42-43	0.18984151481586053
1102	44-45	0.17827801013690703
1102	46-47	0.17201208323993455
1102	48-49	0.16655167123213488
1102	50-51	0.15985032078263828
1102	52-53	0.1399576799000144
1102	54-55	0.15318131129081536
1102	56-57	0.152365894346012
1102	58-59	0.1714404837895387
1102	60-61	0.1354125948036824
1102	62-63	0.1623120107342757
1102	64-65	0.25541076397143314
1102	66-67	0.2935026459473562
1102	68-69	0.282291067874084
1102	70-71	0.20163940745577946
1102	72-73	0.2588558342600251
1102	74-75	0.22456248931424483
1102	76-77	0.22567433050168262
1102	78-79	0.22205209648264201
1102	80-81	0.23443119187398054
1102	82-83	0.15804580857518857
1102	84-85	0.20420538626290607
1102	86-87	0.214025344323872
1102	88-89	0.21271744671238935
1102	90-91	0.19357811342305453
1102	92-93	0.22002637866970431
1102	94-95	0.18711633857107302
1102	96-97	0.18706573404464066
1102	98-99	0.1426601394131488
1102	100	0.18604877438005119
1103	1	0.11625017803254423
1103	2	0.152293105528706
1103	3	0.1355546249180719
1103	4	0.10605394509587995
1103	5	0.1430075485690665
1103	6	0.13342270318307925
1103	7	0.10753102966531713
1103	8	0.1432500802187846
1103	9	0.17155087345022224
1103	10-11	0.21610673468995856
1103	12-13	0.22459241883976944
1103	14-15	0.27261419244435814
1103	16-17	0.30293046033401794
1103	18-19	0.28984566132829315
1103	20-21	0.3027011513141957
1103	22-23	0.2883315373420743
1103	24-25	0.2426063416026807
1103	26-27	0.2898762613402397
1103	28-29	0.2880956160023658
1103	30-31	0.3750155811769389
1103	32-33	0.3914696801083437
1103	34-35	0.41347964866089626
1103	36-37	0.3822847052978773
1103	38-39	0.35886734604367376
1103	40-41	0.3566477434013464
1103	42-43	0.3911048835756574
1103	44-45	0.48967898466516147
1103	46-47	0.569536058900141
1103	48-49	0.5878957508242948
1103	50-51	0.6290711853806599
1103	52-53	0.6441045817138615
1103	54-55	0.6004535876007608
1103	56-57	0.5801704787162265
1103	58-59	0.5679789533587893
1103	60-61	0.5288596800454854
1103	62-63	0.5639237281046334
1103	64-65	0.5995132925438611
1103	66-67	0.633346216509544
1103	68-69	0.608000878390925
1103	70-71	0.5514358270989561
1103	72-73	0.5586211236668248
1103	74-75	0.5570429680618432
1103	76-77	0.509136518969953
1103	78-79	0.4817534360319087
1103	80-81	0.45045225413635137
1103	82-83	0.45285202601735364
1103	84-85	0.482302860601024
1103	86-87	0.4759443019418228
1103	88-89	0.5277078893501894
1103	90-91	0.5710329888166932
1103	92-93	0.6356108583486879
1103	94-95	0.6011224456654176
1103	96-97	0.6438840150861331
1103	98-99	0.6409981201364516
1103	100	0.6290286213483647
1104	1	0.10771967269884897
1104	2	0.14160248531576514
1104	3	0.17159325195073905
1104	4	0.06094803140863547
1104	5	0.0898634535203584
1104	6	0.12280032408557418
1104	7	0.10856489171178652
1104	8	0.1342810388161908
1104	9	0.15724237640682048
1104	10-11	0.21087622699618436
1104	12-13	0.208911781405547
1104	14-15	0.2860729686817436
1104	16-17	0.2944383749312678
1104	18-19	0.282340693722702
1104	20-21	0.3025908312267731
1104	22-23	0.29005604338524904
1104	24-25	0.25970979714615083
1104	26-27	0.284098635534626
1104	28-29	0.24155773660325508
1104	30-31	0.2648176142499139
1104	32-33	0.22570688805512873
1104	34-35	0.2706438589218436
1104	36-37	0.260139054607869
1104	38-39	0.2505875399069879
1104	40-41	0.3008403238540538
1104	42-43	0.3376971546198533
1104	44-45	0.269460880205763
1104	46-47	0.34234025464932216
1104	48-49	0.3111089633287776
1104	50-51	0.2924899957422582
1104	52-53	0.22910803859289075
1104	54-55	0.2398870180267565
1104	56-57	0.2387266064848248
1104	58-59	0.21215706230417197
1104	60-61	0.18437889522509465
1104	62-63	0.22497330939142302
1104	64-65	0.26741616622663145
1104	66-67	0.29767777465911394
1104	68-69	0.29782799419024286
1104	70-71	0.24780887584670808
1104	72-73	0.2638764030684584
1104	74-75	0.22840528209794542
1104	76-77	0.13810856810428618
1104	78-79	0.19254987571179072
1104	80-81	0.23129710109970603
1104	82-83	0.2241344265970966
1104	84-85	0.19099169264061544
1104	86-87	0.16278179825763317
1104	88-89	0.20052485515260798
1104	90-91	0.2704619771668497
1104	92-93	0.2554019161280543
1104	94-95	0.265038794132721
1104	96-97	0.3157144119475568
1104	98-99	0.31980246627221476
1104	100	0.3561234360664187
1105	1	-0.055395031687368146
1105	2	0.02451002939042013
1105	3	0.044285387111258956
1105	4	-0.13217271782455953
1105	5	-0.13026404740075748
1105	6	-0.15025974719590351
1105	7	-0.47700885059487774
1105	8	-0.4366865392035919
1105	9	-0.3584382553315919
1105	10-11	-0.4256692895723262
1105	12-13	-0.2719748277194185
1105	14-15	-0.22702908687585222
1105	16-17	-0.22331997944247206
1105	18-19	-0.2488731366831871
1105	20-21	-0.18919207444137243
1105	22-23	-0.19436185642837245
1105	24-25	-0.23152471942181307
1105	26-27	-0.212601468114741
1105	28-29	-0.23480215817743044
1105	30-31	-0.3047273575720695
1105	32-33	-0.3315570637667662
1105	34-35	-0.33035872238626496
1105	36-37	-0.32592880248751044
1105	38-39	-0.3475159189511743
1105	40-41	-0.330220846391164
1105	42-43	-0.35821243821900595
1105	44-45	-0.267550921907187
1105	46-47	-0.3428383233163643
1105	48-49	-0.38415981016466816
1105	50-51	-0.48387698383948674
1105	52-53	-0.4433641581161609
1105	54-55	-0.38146059855692727
1105	56-57	-0.3348215511299699
1105	58-59	-0.3024059375135124
1105	60-61	-0.23400765698470138
1105	62-63	-0.2557204255863823
1105	64-65	-0.19702523545664263
1105	66-67	-0.20218717701215638
1105	68-69	-0.2640302416724438
1105	70-71	-0.26972509613863593
1105	72-73	-0.21122525654164548
1105	74-75	-0.29594175513478405
1105	76-77	-0.26861000884511554
1105	78-79	-0.20521926795795764
1105	80-81	-0.3050692373895032
1105	82-83	-0.30795516899918596
1105	84-85	-0.2881205289690527
1105	86-87	-0.3573156332378957
1105	88-89	-0.30498398746227195
1105	90-91	-0.292059479854359
1105	92-93	-0.3276488902734762
1105	94-95	-0.4419479489113769
1105	96-97	-0.4027927822217059
1105	98-99	-0.4558958282941461
1105	100	-0.4782404717350026
1106	1	0.1122374142049054
1106	2	0.09985221415376344
1106	3	0.09758598251197981
1106	4	0.06305733543415926
1106	5	0.09424187485925017
1106	6	0.12051335792516937
1106	7	0.09642569252014255
1106	8	0.09704900496155489
1106	9	0.11396949943214452
1106	10-11	0.13817580716816735
1106	12-13	0.09288212852100486
1106	14-15	0.11099883399136701
1106	16-17	0.1921777854003821
1106	18-19	0.15170258587236418
1106	20-21	0.10489072127456467
1106	22-23	0.13050288596552662
1106	24-25	0.123170744777795
1106	26-27	0.08353791731024529
1106	28-29	0.1293071639114345
1106	30-31	0.12357977026503164
1106	32-33	0.06019667793618311
1106	34-35	0.10655998652853782
1106	36-37	0.10659806646594916
1106	38-39	0.06314886433110445
1106	40-41	0.07097254249384122
1106	42-43	0.02752286558652628
1106	44-45	-0.003213394366689215
1106	46-47	0.06075866170252198
1106	48-49	0.06575193598153106
1106	50-51	0.035752923637375034
1106	52-53	0.06242302727787319
1106	54-55	0.09671612738085145
1106	56-57	0.05163466128937699
1106	58-59	0.04078648074080604
1106	60-61	-0.026897080838281795
1106	62-63	-0.006361795935781345
1106	64-65	0.033225741174511825
1106	66-67	0.03659401249896632
1106	68-69	-0.0626688053296931
1106	70-71	0.022096157336747524
1106	72-73	-0.01975516301417457
1106	74-75	-0.0024154293736273758
1106	76-77	0.007185539769736238
1106	78-79	-0.02936318685122785
1106	80-81	-0.017777196544031426
1106	82-83	-9.885362586317115E-4
1106	84-85	0.03842247505969709
1106	86-87	0.046350463681157805
1106	88-89	0.053320786144340104
1106	90-91	0.04376715890199634
1106	92-93	0.026626880847658185
1106	94-95	-0.04152440871727592
1106	96-97	-0.03294802355076243
1106	98-99	0.010939736962043867
1106	100	0.056166785627329574
1107	1	-0.08202832657994463
1107	2	-0.0538271621309363
1107	3	-0.04922805897035687
1107	4	-0.12696740972552334
1107	5	-0.1084357347921312
1107	6	-0.11232983582415557
1107	7	-0.07893958158462056
1107	8	-0.037283866865074344
1107	9	-0.02876904344623199
1107	10-11	0.01796173669440293
1107	12-13	0.010022364580912324
1107	14-15	-0.030000427905243043
1107	16-17	0.007990613442778738
1107	18-19	-0.0027123583396146955
1107	20-21	0.02636714832141962
1107	22-23	0.04464448093474971
1107	24-25	0.03817944476821822
1107	26-27	0.024835007256925223
1107	28-29	-4.436532012945804E-4
1107	30-31	-0.007372347261927814
1107	32-33	-0.019941067216848296
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2216	42-43	-0.704866642882152
2216	44-45	-0.6639636752521412
2216	46-47	-0.6767657019895381
2216	48-49	-0.666269418420292
2216	50-51	-0.6294128294297039
2216	52-53	-0.663327136331354
2216	54-55	-0.6197242805698338
2216	56-57	-0.6317343169095082
2216	58-59	-0.755284818941
2216	60-61	-0.7005484797393393
2216	62-63	-0.7466740909800293
2216	64-65	-0.6475662918248801
2216	66-67	-0.617006083383238
2216	68-69	-0.6760797595335077
2216	70-71	-0.5901666004066399
2216	72-73	-0.6442182688902633
2216	74-75	-0.5472794280416551
2216	76-77	-0.5031136230023776
2216	78-79	-0.5411760792046323
2216	80-81	-0.5373872033336156
2216	82-83	-0.5785954357612582
2216	84-85	-0.5710815149085668
2216	86-87	-0.5520477784903406
2216	88-89	-0.5745721607389491
2216	90-91	-0.5885058996033834
2216	92-93	-0.7121426987295045
2216	94-95	-0.6615406799054036
2216	96-97	-0.792956861025278
2216	98-99	-0.8510320291415354
2216	100	-0.7847251782679407
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60.0
3	418.0
4	271.0
5	447.0
6	668.0
7	1078.0
8	1717.0
9	2612.0
10	3117.0
11	4296.0
12	7323.0
13	11038.0
14	10131.0
15	11972.0
16	16057.0
17	25533.0
18	31412.0
19	16072.0
20	9339.0
21	8065.0
22	7869.0
23	8032.0
24	8798.0
25	10425.0
26	12583.0
27	15765.0
28	19416.0
29	24217.0
30	30215.0
31	38061.0
32	47436.0
33	61667.0
34	89130.0
35	163829.0
36	252144.0
37	547586.0
38	914901.0
39	429875.0
40	3825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53232915914754	20.955773111799665	14.485445609026751	23.026452120026047
2	13.978998384491115	22.225328369740815	40.770035822153545	23.025637423614526
3	15.485760643958152	30.03013285851253	31.48075957280176	23.00334692472756
4	10.35870999826503	18.705945322503943	39.647822474093125	31.287522205137897
5	11.105640233195196	38.97650488164641	35.664255109924845	14.253599775233546
6	26.703589239306037	40.126957926529464	19.14251597949006	14.02693685467444
7	24.001194071784788	34.436538596614454	24.60360328720938	16.958664044391377
8	24.990939102338977	34.10637774812109	21.775022827842943	19.127660321696986
9	26.29040528201166	15.355341715248999	22.76329282854534	35.590960174194
10-11	23.08658776427618	26.9016997963054	29.873200112383223	20.13851232703519
12-13	24.661094331671	25.970394043688977	29.723747980613897	19.644763644026128
14-15	21.97102619933975	26.15538737093489	29.597439769614386	22.276146660110978
16-17	19.643674931516472	29.256110837957433	29.415484301468002	21.68472992905809
18-19	19.560142586219005	26.89051415326262	34.09238252440823	19.456960736110133
20-21	21.050168574840207	25.819870759289177	34.09259324295849	19.03736742291213
22-23	20.49099897836587	26.03476014425797	34.000538737282696	19.47370214009347
24-25	20.02364966190185	26.41250011413929	33.74961982834855	19.81423039561031
26-27	19.768914446824404	26.413989387462962	33.94531547696186	19.871780688750775
28-29	20.08957365154427	26.102018436262842	34.23643334963488	19.571974562558005
30-31	20.28790283735804	26.353965328106018	33.91043143807952	19.447700396456426
32-33	19.636342435806416	26.22917454337344	34.11628310872902	20.018199912091124
34-35	20.19920923906601	26.115990562848634	33.80142119214718	19.88337900593817
36-37	20.654425492997515	26.24724473496774	33.06414622256501	20.034183549469738
38-39	20.38611423100616	26.317836644870717	33.240438897235926	20.055610226887197
40-41	20.076939583795028	26.396806236366466	33.34210773087056	20.18414644896795
42-43	20.231067912065374	26.42685155658449	33.1963539917988	20.145726539551337
44-45	20.280327141985417	26.47435587885386	32.77521938970372	20.470097589457005
46-47	20.80854745551361	26.580956332895315	32.05158845904555	20.558907752545522
48-49	20.87177490714581	26.510598223148936	31.773368868247402	20.844258001457852
50-51	20.963775914891393	26.802999325518762	31.102487959517784	21.130736800072068
52-53	20.81990587904755	27.055138020650414	30.972220271124534	21.152735829177498
54-55	20.961365124569408	27.48341155413109	30.915135043918312	20.640088277381185
56-57	20.967139230245945	27.060378727509566	30.893854238479832	21.078627803764658
58-59	21.11202228013121	26.306429068125787	31.20638973372012	21.375158918022883
60-61	21.302462271169947	26.73248480231819	31.0970058033818	20.868047123130065
62-63	20.80301327526867	26.76036032872094	31.34245276392498	21.09417363208541
64-65	20.872146519631947	26.83109152209033	31.345560862541266	20.95120109573646
66-67	21.070157354906343	26.534574706265573	31.09068496181584	21.30458297701224
68-69	21.371428691839974	26.848918356904118	30.861763845442532	20.917889105813376
70-71	21.42426415635062	26.57915587298198	31.034658966391138	20.961921004276267
72-73	21.146544618439496	26.62092667729391	30.966629545069026	21.26589915919757
74-75	21.044210078253506	26.717224814595053	31.04602404658229	21.19254106056916
76-77	21.135693883550076	26.97857720078093	30.67435160728064	21.211377308388357
78-79	21.172110949188124	27.039847747513456	30.748649062970447	21.039392240327977
80-81	20.891726277105658	27.1049731401824	30.85983375571052	21.143466827001422
82-83	20.756618440232405	27.076089432112095	30.863009731975144	21.30428239568036
84-85	20.88392384275746	26.80886094265168	30.657673110503296	21.64954210408756
86-87	21.058361661893	26.922992500328807	30.607557829683763	21.411088008094424
88-89	20.68920890167582	26.849512608471414	30.781816013934353	21.67946247591841
90-91	20.622031994361066	26.861475012428244	30.690093632823977	21.826399360386716
92-93	20.594492991121115	27.016292882035962	30.918159095035787	21.471055031807136
94-95	20.742430542775704	26.636636552263777	30.83181496991603	21.789117935044484
96-97	20.722352402385948	27.038818039037192	30.554244388438878	21.684585170137982
98-99	20.258939422700898	26.811639121420285	30.8145779990521	22.11484345682672
100	20.410513494833022	26.860210612676095	30.771338019858728	21.95793787263215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6736.0
1	4293.0
2	9195.5
3	12560.5
4	5257.5
5	2409.0
6	4752.5
7	12219.0
8	19919.5
9	19033.5
10	10753.0
11	4334.0
12	3165.0
13	3136.5
14	3320.5
15	3583.0
16	3799.0
17	4266.0
18	4789.5
19	5205.5
20	6186.5
21	7397.5
22	8716.5
23	10743.5
24	13755.0
25	17166.5
26	21337.0
27	26745.0
28	32940.0
29	39346.0
30	44712.5
31	50035.0
32	55549.0
33	59418.0
34	63290.0
35	71019.0
36	77999.5
37	84511.5
38	94090.5
39	99064.5
40	102533.5
41	106882.0
42	108145.0
43	111682.5
44	112587.0
45	108234.0
46	105079.5
47	102988.5
48	96251.5
49	91907.0
50	92943.0
51	86503.5
52	74895.0
53	66593.5
54	60744.0
55	55701.0
56	49560.0
57	42834.0
58	39978.5
59	38307.5
60	34379.0
61	28982.5
62	23945.0
63	19253.5
64	14897.5
65	11547.0
66	9038.5
67	7019.0
68	5385.5
69	4047.0
70	2999.0
71	2258.0
72	1682.0
73	1205.0
74	900.5
75	696.5
76	481.5
77	341.5
78	243.0
79	141.0
80	69.5
81	38.5
82	23.5
83	15.5
84	9.5
85	4.0
86	3.0
87	3.5
88	3.0
89	4.5
90	5.0
91	2.5
92	2.5
93	1.5
94	4.0
95	8.5
96	8.5
97	9.5
98	6.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007656107325981596
2	0.0
3	3.511975837606237E-4
4	0.0030202992203413642
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.5119758376062375E-5
24-25	7.023951675212475E-5
26-27	4.565568588888108E-4
28-29	7.901945634614034E-4
30-31	0.0017735477979911498
32-33	0.004688487743204327
34-35	0.0050045655685888885
36-37	0.004354850038631734
38-39	0.0028798201868371143
40-41	0.0035470955959822996
42-43	4.214371005127485E-4
44-45	4.389969797007797E-4
46-47	0.003389056683290019
48-49	0.002089625623375711
50-51	9.306735969656529E-4
52-53	0.0
54-55	4.0387722132471726E-4
56-57	8.077544426494345E-4
58-59	7.023951675212474E-4
60-61	2.985179461965302E-4
62-63	0.0
64-65	0.0
66-67	5.267963756409356E-4
68-69	6.672754091451851E-4
70-71	0.001966706469059493
72-73	3.5119758376062375E-5
74-75	0.0018086675563672122
76-77	4.565568588888108E-4
78-79	8.604340802135281E-4
80-81	8.42874201025497E-4
82-83	0.0011765119055980894
84-85	9.833532345297465E-4
86-87	2.985179461965302E-4
88-89	0.001703308281239025
90-91	0.001914026831495399
92-93	0.002914939945213177
94-95	0.0012643113015382453
96-97	0.002247664536067992
98-99	0.001914026831495399
100	0.0030905387370934886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2847400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.949283105403794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89145815973892	36.65883187637563
2	14.665426800351277	14.357242566239414
3	4.575298864420948	6.7187279821912025
4	1.9755880098964247	3.8681446718424537
5	1.0247625147814838	2.5080695225922196
6	0.6031995718899096	1.771571196809853
7	0.42727765011733665	1.4640454262143625
8	0.293553359897447	1.1495381216130098
9	0.20483612378856794	0.9023923272186131
>10	1.1352311818947607	10.422533075473666
>50	0.1109476852371706	3.8304669970828957
>100	0.08135309577661863	7.7433413157970685
>500	0.00754566968809477	2.569902584003271
>1k	0.003018267875227032	2.0759164911227512
>5k	2.1559056251621662E-4	0.7445495721541354
>10k+	2.8745408335495545E-4	3.214726273269495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31040	1.090117299992976	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21608	0.7588677389899557	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19586	0.6878555875535576	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19154	0.6726838519350986	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7876	0.2766032169698672	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7511	0.2637845051626045	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5779	0.20295708365526444	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3547	0.12456978295989324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	3.5119758376062375E-5	0.0
18-19	0.0	0.0	0.0	7.023951675212475E-5	0.0
20-21	0.0	0.0	0.0	1.7559879188031187E-4	0.0
22-23	0.0	0.0	0.0	7.550748050853411E-4	0.0
24-25	0.0	0.0	0.0	0.0015803891269228069	0.0
26-27	0.0	0.0	0.0	0.00237058369038421	0.0
28-29	0.0	0.0	0.0	0.0050045655685888885	0.0
30-31	0.0	0.0	0.0	0.010272529324998244	0.0
32-33	0.0	0.0	0.0	0.019596825173842803	0.0
34-35	0.0	0.0	0.0	0.03208189927653298	0.0
36-37	0.0	0.0	0.0	0.05085341012853832	0.0
38-39	0.0	0.0	0.0	0.07786050431973028	0.0
40-41	0.0	0.0	0.0	0.10678162534241764	0.0
42-43	0.0	0.0	0.0	0.13528130926459225	0.0
44-45	0.0	0.0	0.0	0.16778464564163798	0.0
46-47	0.0	0.0	0.0	0.20759289176090467	0.0
48-49	0.0	0.0	0.0	0.25047411673807685	0.0
50-51	0.0	3.5119758376062375E-5	0.0	0.29802626957926526	0.0
52-53	0.0	3.5119758376062375E-5	0.0	0.34387511413921473	0.0
54-55	0.0	3.5119758376062375E-5	0.0	0.39277937767788157	0.0
56-57	0.0	5.267963756409356E-5	0.0	0.45204396993748686	0.0
58-59	0.0	7.023951675212475E-5	0.0	0.520264100582988	0.0
60-61	0.0	1.0535927512818712E-4	0.0	0.5964739762590433	0.0
62-63	0.0	1.0535927512818712E-4	0.0	0.6728418908477909	0.0
64-65	0.0	1.0535927512818712E-4	0.0	0.756198637353375	0.0
66-67	0.0	1.404790335042495E-4	0.0	0.8549905176652384	0.0
68-69	0.0	1.404790335042495E-4	0.0	0.9702535646554752	0.0
70-71	0.0	1.404790335042495E-4	0.0	1.1003722694387863	0.0
72-73	0.0	1.404790335042495E-4	0.0	1.2393587132120532	0.0
74-75	0.0	1.404790335042495E-4	0.0	1.3850354709559598	0.0
76-77	0.0	1.404790335042495E-4	0.0	1.5328369740816183	0.0
78-79	0.0	1.404790335042495E-4	0.0	1.7036243590644096	0.0
80-81	0.0	1.404790335042495E-4	0.0	1.899171173702325	0.0
82-83	0.0	1.404790335042495E-4	0.0	2.111101355622673	0.0
84-85	0.0	1.404790335042495E-4	0.0	2.332215354358362	0.0
86-87	0.0	1.404790335042495E-4	0.0	2.5605464634403314	0.0
88	0.0	1.404790335042495E-4	0.0	2.7497366018121796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8565	0.0	57.84158	1
GTATCAA	17250	0.0	46.131203	1
TAACGCA	240	0.0	43.081673	4
TACACGG	225	0.0	41.77617	2
TATAACG	260	0.0	39.7677	2
TACCTGG	2085	0.0	36.291172	2
GTACACG	255	0.0	35.021946	1
ATCAACG	23545	0.0	33.65426	3
TCAACGC	23845	0.0	33.250553	4
CAACGCA	24480	0.0	32.330456	5
TATCAAC	25070	0.0	31.700815	2
AACGCAG	25050	0.0	31.594793	6
GTACCTG	2485	0.0	31.587626	1
ATAACGC	430	0.0	29.51049	3
ACGCAGA	27835	0.0	28.332304	7
CGCAGAG	28065	0.0	28.01638	8
ACCTGGG	2645	0.0	27.896847	3
GCAGAGT	29800	0.0	26.1802	9
GTGGTAT	3155	0.0	25.922495	1
TACATGG	14955	0.0	24.198332	2
>>END_MODULE