FastQCFastQC Report
Wed 25 May 2016
SRR2431349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949363
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA818372.7747347478082554No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86730.2940634977790119No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.14172551835769281No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38510.13057056727164476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.1171778448431068No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33860.11480445099501146No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31690.10744693006591592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.048.801951
GTATCAA86100.037.6108251
TCAACGC99400.031.2071634
ATCAACG100500.031.0058943
TATCAAC111500.028.4949952
CAACGCA115150.027.1836055
AACGCAG118050.026.436196
ACGCAGA118650.026.1044437
GTACATG174750.025.5507221
CGCAGAG120800.025.5231158
TACATGG172550.024.8141962
ACATGGG177900.023.1168613
GCAGAGT131700.022.8754189
CATGGGG107450.020.5146054
GCCCATA17300.020.3756711
CAGAGTA127500.019.99798810-11
GTACGAC1456.603366E-419.4482273
TATATCG1550.001033864318.1935025
AGAGTAC125950.017.89316710-11
TTACGGT1851.6508193E-417.783744
Produced by FastQC (version 0.11.5)