##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431349_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2949363
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.430433283390347	33.0	31.0	34.0	26.0	34.0
2	30.641621936669036	33.0	31.0	34.0	26.0	34.0
3	30.828395826488634	34.0	31.0	34.0	26.0	34.0
4	33.85735157049166	37.0	35.0	37.0	28.0	37.0
5	34.19236357138813	37.0	35.0	37.0	30.0	37.0
6	34.0982090030966	37.0	35.0	37.0	30.0	37.0
7	34.11948376649466	37.0	35.0	37.0	30.0	37.0
8	34.02466566509446	37.0	35.0	37.0	29.0	37.0
9	35.40797724796846	39.0	37.0	39.0	27.0	39.0
10-11	35.494911104533415	39.0	37.0	39.0	29.0	39.0
12-13	35.43423969853829	39.0	37.0	39.0	28.5	39.0
14-15	36.734618458290825	41.0	38.0	41.0	28.0	41.0
16-17	36.73320510225429	40.5	38.0	41.0	29.0	41.0
18-19	36.66208008305522	40.0	38.0	41.0	29.0	41.0
20-21	36.64908609079316	40.0	38.0	41.0	28.0	41.0
22-23	36.63518359727168	40.0	38.0	41.0	27.5	41.0
24-25	36.62480067729879	40.0	37.0	41.0	27.5	41.0
26-27	36.60313396485953	40.0	37.0	41.0	28.0	41.0
28-29	36.56574131431092	40.0	37.0	41.0	28.0	41.0
30-31	36.477582786520344	40.0	37.0	41.0	27.5	41.0
32-33	36.38229983898218	40.0	37.0	41.0	27.0	41.0
34-35	36.297850756248046	40.0	36.0	41.0	27.0	41.0
36-37	36.17699076037775	40.0	36.0	41.0	27.0	41.0
38-39	36.05868199336602	40.0	36.0	41.0	26.0	41.0
40-41	35.94499863190798	40.0	35.5	41.0	26.0	41.0
42-43	35.789848350304794	40.0	35.0	41.0	25.0	41.0
44-45	35.63077434008632	40.0	35.0	41.0	25.0	41.0
46-47	35.473432907376946	40.0	35.0	41.0	25.0	41.0
48-49	35.30797497629149	39.5	35.0	41.0	25.0	41.0
50-51	35.13047376670827	39.0	35.0	41.0	25.0	41.0
52-53	34.91798568029775	39.0	35.0	41.0	25.0	41.0
54-55	34.73107515080375	39.0	34.0	41.0	25.0	41.0
56-57	34.50529809318148	38.0	34.0	40.5	25.0	41.0
58-59	34.25220428953642	38.0	34.0	40.0	25.0	41.0
60-61	34.039566170729074	37.0	33.5	40.0	25.0	41.0
62-63	33.84677911806719	37.0	33.0	40.0	25.0	41.0
64-65	33.57955073688793	36.0	33.0	40.0	24.0	41.0
66-67	33.27420412475507	36.0	33.0	39.5	23.0	41.0
68-69	32.969238272806706	35.0	33.0	39.0	22.5	41.0
70-71	32.736126411025026	35.0	33.0	39.0	23.0	41.0
72-73	32.505477793001404	35.0	33.0	38.5	22.0	40.5
74-75	32.187304004288386	35.0	32.5	37.0	20.5	40.0
76-77	31.888003104399154	35.0	32.0	37.0	20.0	39.0
78-79	31.590041307224645	35.0	32.0	36.5	19.5	39.0
80-81	31.302513797047023	35.0	32.0	36.0	18.0	39.0
82-83	31.02376835269175	35.0	32.0	36.0	17.0	37.0
84-85	30.777899838032823	35.0	31.0	35.0	13.5	37.0
86-87	30.55761549188757	35.0	31.0	35.0	9.0	36.5
88-89	30.34487684289794	35.0	31.0	35.0	6.0	36.0
90-91	30.160262063367583	35.0	31.0	35.0	2.0	36.0
92-93	29.98107489651155	35.0	31.0	35.0	2.0	36.0
94-95	29.775360815199758	35.0	31.0	35.0	2.0	35.5
96-97	29.608208789491155	35.0	30.0	35.0	2.0	35.0
98-99	29.496175445341926	34.5	30.5	35.0	2.0	35.0
100	29.45749234665248	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19909735317129673
1101	2	0.2756968456230737
1101	3	0.26867885067981234
1101	4	0.3295084835092936
1101	5	0.14486104876424122
1101	6	0.21643552386287013
1101	7	0.20523423515663097
1101	8	0.20490854079495335
1101	9	0.30449774341878566
1101	10-11	0.25638932310416607
1101	12-13	0.2722520286389454
1101	14-15	0.3331840614661843
1101	16-17	0.2999843306842678
1101	18-19	0.2888758404277283
1101	20-21	0.2025206252412346
1101	22-23	0.2615267569764086
1101	24-25	0.2630245469402439
1101	26-27	0.29361787216534907
1101	28-29	0.2699340186447401
1101	30-31	0.3416056699651122
1101	32-33	0.31889233562610286
1101	34-35	0.3192141372691495
1101	36-37	0.2869460547400564
1101	38-39	0.26634013665136536
1101	40-41	0.2557692918669474
1101	42-43	0.2989922993939871
1101	44-45	0.2879187939847867
1101	46-47	0.2673243786288282
1101	48-49	0.2397884068246654
1101	50-51	0.2419799256493178
1101	52-53	0.2895817101204159
1101	54-55	0.3417098460198389
1101	56-57	0.31837121442585214
1101	58-59	0.31240560965380126
1101	60-61	0.33757928669731285
1101	62-63	0.37816641062415357
1101	64-65	0.41521674361590755
1101	66-67	0.34057558312701275
1101	68-69	0.3146682012656399
1101	70-71	0.3982875013083813
1101	72-73	0.43883653315489113
1101	74-75	0.41922927262754683
1101	76-77	0.3997693581393058
1101	78-79	0.3492504181399454
1101	80-81	0.32050029100343735
1101	82-83	0.34666750498805854
1101	84-85	0.35226077392988486
1101	86-87	0.31237031988277764
1101	88-89	0.34909746397433494
1101	90-91	0.3301421319286817
1101	92-93	0.40014112538783664
1101	94-95	0.4503947815270095
1101	96-97	0.41309017477130183
1101	98-99	0.44825024620665843
1101	100	0.3874381230273656
1102	1	0.13309007193618427
1102	2	0.22545925213123397
1102	3	0.24215439421727325
1102	4	0.2647439491414616
1102	5	0.20657203998915463
1102	6	0.18504216678208962
1102	7	0.26241529491897353
1102	8	0.30664886053384777
1102	9	0.31720518573026624
1102	10-11	0.3201608362875632
1102	12-13	0.3539173982644712
1102	14-15	0.3708215808865418
1102	16-17	0.4301339626404683
1102	18-19	0.3545152849144202
1102	20-21	0.3467642141900029
1102	22-23	0.336861661563006
1102	24-25	0.28618523866538226
1102	26-27	0.35587824788937183
1102	28-29	0.3520951486651569
1102	30-31	0.37693600254918636
1102	32-33	0.3800285556746985
1102	34-35	0.31965318274688315
1102	36-37	0.38520380849444535
1102	38-39	0.3699329936446105
1102	40-41	0.4095231922178115
1102	42-43	0.43014134421576244
1102	44-45	0.4186022363437871
1102	46-47	0.39639795756155394
1102	48-49	0.28256255728171453
1102	50-51	0.23327616638655257
1102	52-53	0.19509969946197003
1102	54-55	0.296360178150195
1102	56-57	0.3394848877442129
1102	58-59	0.3309612001452109
1102	60-61	0.3215146513769227
1102	62-63	0.38002813029365967
1102	64-65	0.2994965568923931
1102	66-67	0.3994659524719637
1102	68-69	0.4038224006031541
1102	70-71	0.33336776611962904
1102	72-73	0.31123214064757576
1102	74-75	0.28293357413292597
1102	76-77	0.30712806162549455
1102	78-79	0.2702752861007127
1102	80-81	0.3867813942592129
1102	82-83	0.29499559424209565
1102	84-85	0.33563409077968487
1102	86-87	0.3589015785344287
1102	88-89	0.30423551438435226
1102	90-91	0.35898601844771605
1102	92-93	0.4059536842070557
1102	94-95	0.3742391910836105
1102	96-97	0.4120183228528056
1102	98-99	0.33561054971368875
1102	100	0.389136269673898
1103	1	0.033880807984175476
1103	2	0.0844762618287298
1103	3	0.15515241206322727
1103	4	0.14715560691556107
1103	5	0.06906489455010956
1103	6	0.14100596187090275
1103	7	0.09666170798917761
1103	8	0.2361971899728914
1103	9	0.27083025637860914
1103	10-11	0.307424908428942
1103	12-13	0.2846936028150253
1103	14-15	0.2619477486516004
1103	16-17	0.3233802076950809
1103	18-19	0.3083906175870723
1103	20-21	0.267480380931687
1103	22-23	0.18406461997479084
1103	24-25	0.2529839195698571
1103	26-27	0.30677984198353414
1103	28-29	0.2989770265420617
1103	30-31	0.2038007158485584
1103	32-33	0.22688375047903975
1103	34-35	0.23154894907649037
1103	36-37	0.29217040109652714
1103	38-39	0.24668573834701846
1103	40-41	0.22035188044358023
1103	42-43	0.19862538745177005
1103	44-45	0.1445692305353674
1103	46-47	0.12463561017721503
1103	48-49	0.11662602800610244
1103	50-51	0.03984720587284585
1103	52-53	0.10858468786030073
1103	54-55	0.16813443111670523
1103	56-57	0.08890396817709956
1103	58-59	0.059420992773134174
1103	60-61	0.029836366716970986
1103	62-63	0.12022160854232311
1103	64-65	0.18036863899481403
1103	66-67	0.13560586061627333
1103	68-69	0.2010351390267644
1103	70-71	0.17421037324867683
1103	72-73	0.08324358576911095
1103	74-75	0.1981708146506449
1103	76-77	0.09212515926667209
1103	78-79	0.11394481221674724
1103	80-81	0.1544605269978554
1103	82-83	0.17175204078047912
1103	84-85	0.08404430149415631
1103	86-87	0.1398957674878396
1103	88-89	0.14079656010764197
1103	90-91	0.12489304647885291
1103	92-93	0.1258085591011202
1103	94-95	0.07282663018094482
1103	96-97	0.016115920922505467
1103	98-99	0.10495521010785325
1103	100	0.08758112837899645
1104	1	0.08775855078512862
1104	2	0.1293221803932454
1104	3	0.06999155722686012
1104	4	0.14695210114906132
1104	5	0.12391063320711027
1104	6	0.18645889304319496
1104	7	0.0823071915980833
1104	8	0.13780355374540676
1104	9	0.18439100682189746
1104	10-11	0.18129934747382492
1104	12-13	0.2508280586777545
1104	14-15	0.2121026793907177
1104	16-17	0.13073758368098254
1104	18-19	0.14561778025640137
1104	20-21	0.07981745657484396
1104	22-23	0.16863147629200625
1104	24-25	0.172480040693884
1104	26-27	0.1814191799986702
1104	28-29	0.19681508735408215
1104	30-31	0.22692782611106566
1104	32-33	0.2343666762050418
1104	34-35	0.24958000391627166
1104	36-37	0.1837184164246537
1104	38-39	0.21941947032735243
1104	40-41	0.19604877087078876
1104	42-43	0.18348944162776348
1104	44-45	0.23630494547028746
1104	46-47	0.24871402228082928
1104	48-49	0.15909950391496608
1104	50-51	0.12184723764871563
1104	52-53	0.1816682581692035
1104	54-55	0.11605716528093524
1104	56-57	0.14192703348796698
1104	58-59	0.1761012926906389
1104	60-61	0.1855823555388625
1104	62-63	0.1788989682571085
1104	64-65	0.20123253616061731
1104	66-67	0.2320839491667499
1104	68-69	0.20112082873958315
1104	70-71	0.16088693716478275
1104	72-73	0.11090156150122255
1104	74-75	0.19940318108625377
1104	76-77	0.24265850325427252
1104	78-79	0.296251516201373
1104	80-81	0.31607237678116107
1104	82-83	0.27282442972088816
1104	84-85	0.23289714579682652
1104	86-87	0.2182055341057456
1104	88-89	0.2683550142769029
1104	90-91	0.3460212791773003
1104	92-93	0.29767846634594974
1104	94-95	0.34199145279564647
1104	96-97	0.2565547130001242
1104	98-99	0.2505252096662609
1104	100	0.2844092889041612
1105	1	0.053629473395211846
1105	2	0.15922973894382153
1105	3	0.17100384222885268
1105	4	0.17461764130953839
1105	5	0.12646868527163946
1105	6	0.14922596127563992
1105	7	0.19986316760116551
1105	8	0.1430510622680785
1105	9	0.23693926899733952
1105	10-11	0.17959865236261408
1105	12-13	0.25321587835719583
1105	14-15	0.2468866846548039
1105	16-17	0.23440590689227747
1105	18-19	0.1904842951477903
1105	20-21	0.18137490402791911
1105	22-23	0.17059149003391383
1105	24-25	0.16388931122440198
1105	26-27	0.19825933071293633
1105	28-29	0.18528544248797374
1105	30-31	0.13199295068696415
1105	32-33	0.1476832870350222
1105	34-35	0.14035200676470794
1105	36-37	0.19109084858560266
1105	38-39	0.18154347721785768
1105	40-41	0.15506752732103024
1105	42-43	0.19519214008882102
1105	44-45	0.21483696352649417
1105	46-47	0.12561372902623447
1105	48-49	0.0663900686374248
1105	50-51	0.09665970862951667
1105	52-53	0.16461471346733703
1105	54-55	0.16345122245179056
1105	56-57	0.1025863961877107
1105	58-59	0.056234396790486585
1105	60-61	0.06986764070152418
1105	62-63	0.14739739704680943
1105	64-65	0.04798781906514904
1105	66-67	0.10299053361463706
1105	68-69	0.08498028572008565
1105	70-71	0.10376214906091974
1105	72-73	0.06392911524003608
1105	74-75	0.09347381813173428
1105	76-77	0.12850647692173212
1105	78-79	0.07534895125043306
1105	80-81	0.004320688156173702
1105	82-83	0.0773371555878235
1105	84-85	0.10911010607430782
1105	86-87	0.14841554962181647
1105	88-89	0.1889968238160371
1105	90-91	0.09723495202840837
1105	92-93	0.04833208541141687
1105	94-95	0.08201966838447561
1105	96-97	0.09771640927669623
1105	98-99	0.13651854835349653
1105	100	0.16016297366600796
1106	1	0.07309689578220357
1106	2	0.037949635322785724
1106	3	0.14268386419294288
1106	4	0.12541745116304526
1106	5	0.19195685011220576
1106	6	0.19346206792210552
1106	7	0.23644291562593622
1106	8	0.17522602537594878
1106	9	0.1242568113850524
1106	10-11	0.15530852741181178
1106	12-13	0.20299130028094226
1106	14-15	0.21885000514318875
1106	16-17	0.24926947334968474
1106	18-19	0.16101115956495704
1106	20-21	0.1298337579090969
1106	22-23	0.11712754708301532
1106	24-25	0.07979038138337557
1106	26-27	0.12212407318926921
1106	28-29	0.1796398379405133
1106	30-31	0.20068519554146746
1106	32-33	0.16716463553814265
1106	34-35	0.20553063080432565
1106	36-37	0.18235388775035233
1106	38-39	0.17703935803906745
1106	40-41	0.18589804332146542
1106	42-43	0.186859170784345
1106	44-45	0.19676419202975381
1106	46-47	0.11961269994259993
1106	48-49	0.1397351657375978
1106	50-51	0.10962081736087725
1106	52-53	0.10556358052546955
1106	54-55	0.13262599247231321
1106	56-57	0.10786831176013578
1106	58-59	0.09850898717215983
1106	60-61	0.15851343404311535
1106	62-63	0.13633286970348024
1106	64-65	0.15499601491989523
1106	66-67	0.15138877803315864
1106	68-69	0.17901961822389723
1106	70-71	0.17600233361763884
1106	72-73	0.0804759707981404
1106	74-75	0.11192192394918266
1106	76-77	0.08727846502480574
1106	78-79	0.09401316677755034
1106	80-81	0.1197582091593894
1106	82-83	0.1406895801326904
1106	84-85	0.13150756719575796
1106	86-87	0.14250414140114742
1106	88-89	0.18016946397260014
1106	90-91	0.18221007338418005
1106	92-93	0.20826250098282628
1106	94-95	0.2193428992820543
1106	96-97	0.1491405116217912
1106	98-99	0.16986617630391265
1106	100	0.20572020989597561
1107	1	0.1701530396665305
1107	2	0.16029247653734657
1107	3	0.21082540149417994
1107	4	0.25973678758640517
1107	5	0.21538555011858307
1107	6	0.21419821426284358
1107	7	0.2744852992858071
1107	8	0.23571727896641193
1107	9	0.27788220185618684
1107	10-11	0.2785161743371489
1107	12-13	0.22708018496919635
1107	14-15	0.30274117266328204
1107	16-17	0.34711557914523894
1107	18-19	0.38864718457237757
1107	20-21	0.3538872836997484
1107	22-23	0.3926943089516328
1107	24-25	0.3280753788957753
1107	26-27	0.3646313452719028
1107	28-29	0.3956032767444384
1107	30-31	0.3562924114432775
1107	32-33	0.41927225612421637
1107	34-35	0.4525627143261701
1107	36-37	0.43004944236449205
1107	38-39	0.44161012644980246
1107	40-41	0.48780221831011517
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2216	64-65	-0.8024259514796412
2216	66-67	-0.8488740290548265
2216	68-69	-0.8528340306889746
2216	70-71	-0.909578989710166
2216	72-73	-0.8813273466005
2216	74-75	-0.8872449616717333
2216	76-77	-0.8623605559368634
2216	78-79	-0.787318326871727
2216	80-81	-0.8032500792542301
2216	82-83	-0.8093644070014783
2216	84-85	-0.7794977787041582
2216	86-87	-0.761973695483487
2216	88-89	-0.8567010336041356
2216	90-91	-0.7988433104859141
2216	92-93	-0.919946239461467
2216	94-95	-0.8853162887504524
2216	96-97	-0.9640514041272006
2216	98-99	-1.0255909080156087
2216	100	-1.0351977895815097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66364.0
3	18538.0
4	16424.0
5	17050.0
6	9535.0
7	6794.0
8	6795.0
9	6670.0
10	6501.0
11	6983.0
12	7808.0
13	8684.0
14	8512.0
15	8333.0
16	8698.0
17	8786.0
18	8879.0
19	8321.0
20	8160.0
21	8697.0
22	10835.0
23	20398.0
24	38707.0
25	39063.0
26	44376.0
27	32146.0
28	31249.0
29	31454.0
30	36417.0
31	43734.0
32	58677.0
33	77392.0
34	110992.0
35	204204.0
36	344509.0
37	597020.0
38	698061.0
39	282185.0
40	1412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52073610845516	23.423685506084734	13.027862429255551	22.027715956204556
2	15.50675926531833	24.751005218422037	36.567335478658244	23.17490003760139
3	17.371768790517265	31.422749342230176	27.96233759188434	23.243144275368216
4	11.995491900271178	21.70696131158035	36.33878966202136	29.95875712612711
5	12.592833699514472	42.37702416795508	31.913093010008954	13.117049122521497
6	27.409579627872187	41.479160076260534	17.715384644073993	13.395875651793284
7	24.770094423778964	37.22264773783356	21.43293314522492	16.574324693162556
8	23.862135654376896	38.66834974196123	19.373369775100592	18.096144828561286
9	25.02855701383655	21.16334950970769	19.76796345515964	34.04013002129612
10-11	22.94017386127106	31.226183416554694	26.285252103589823	19.548390618584417
12-13	23.355890746578158	29.77941338519538	25.97784674182188	20.886849126404584
14-15	21.67525665711545	30.446523537455377	23.94986646269042	23.928353342738752
16-17	21.13742865832385	33.05162843637762	24.564524610907508	21.246418294391027
18-19	21.1735551032545	32.056277914926035	26.149121013588356	20.621045968231105
20-21	23.031210468158715	30.688626662774297	25.427592330954173	20.852570538112804
22-23	22.67606598441765	30.99281099003412	25.36740984409176	20.96371318145647
24-25	21.796130215236307	31.093544606072566	25.62215976805839	21.488165410632735
26-27	21.707772152834355	31.38713342508196	25.613225635501628	21.291868786582054
28-29	21.489860691952806	31.14275862279414	26.172278556420487	21.195102128832566
30-31	21.9353636700535	31.146589958577497	25.65306474652323	21.26498162484577
32-33	21.476128913260254	31.207942189550756	25.79984220321473	21.51608669397426
34-35	22.12415019785628	30.721294733812016	25.82037206000075	21.33418300833095
36-37	21.88916725408164	30.947004488765877	25.68749591013382	21.476332347018662
38-39	21.717926887941566	30.85222131016087	25.701041207881158	21.728810594016405
40-41	21.785721187931088	30.760608307624395	25.848530682727084	21.605139821717433
42-43	21.723928183814607	31.012645781478916	25.97137076717922	21.29205526752726
44-45	21.674849789598635	30.833573215640126	25.74537281440094	21.7462041803603
46-47	21.810404483951277	30.654059876658113	25.842868443118057	21.69266719627255
48-49	21.579235923146793	30.69466186427374	25.835866931266178	21.890235281313288
50-51	21.609886609413625	30.888517283223532	25.644469670230485	21.857126437132358
52-53	21.63838428840397	30.851119377302826	25.63665442334497	21.873841910948226
54-55	21.66506801638184	30.850339547895594	25.69605708080016	21.788535354922402
56-57	21.648166061620763	30.872920695078903	25.57464103265688	21.904272210643448
58-59	22.011702187896166	30.384052420810864	25.677798900983024	21.926446490309942
60-61	21.96426821656066	30.764015823077727	25.552907526133612	21.718808434228002
62-63	21.43422155902817	30.811534558479238	25.857261381525436	21.89698250096716
64-65	22.065730803566737	30.692661432316065	25.54341395074123	21.698193813375973
66-67	21.742932287412568	30.651279615293202	25.62405848313687	21.98172961415736
68-69	21.84127555678972	30.776645668912234	25.73793052940584	21.644148244892204
70-71	21.902102250553764	30.67269101836566	25.699396785000694	21.72580994607988
72-73	21.855617636757497	30.711716394353626	25.65130165395036	21.781364314938514
74-75	21.912223080034572	30.609084063236708	25.718451747038255	21.760241109690465
76-77	21.891133780412922	30.442844776990828	25.4008068860971	22.26521455649915
78-79	21.817677240814373	30.446014953059358	25.807810025419048	21.928497780707225
80-81	21.488657042215557	30.85591702343862	25.905356512575768	21.750069421770057
82-83	21.58898879756964	30.92703162720048	25.84321683348252	21.640762741747363
84-85	21.639282898284687	30.336266244923237	25.864848952029853	22.159601904762226
86-87	22.00270705733645	30.596919026978348	25.608630169313145	21.791743746372056
88-89	21.615139040079505	30.603787269110665	25.72679290218903	22.054280788620797
90-91	21.634907013118763	30.529565281371397	25.562415594006282	22.27311211150356
92-93	21.58000399415592	30.60137137207393	25.882689809714748	21.935934824055398
94-95	21.664168055835518	30.306524705562037	25.98854204828899	22.04076519031345
96-97	21.738054280162867	30.830385892019812	25.486813099812007	21.944746728005317
98-99	21.257510053668728	30.784731206238504	25.90666960748097	22.051089132611796
100	21.442632933779272	30.712022698359743	25.69518473611258	22.15015963174841
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75448.0
1	52564.0
2	23031.5
3	12103.5
4	5802.5
5	3248.5
6	2705.0
7	3345.0
8	4653.5
9	4989.0
10	3338.5
11	1693.5
12	1336.0
13	1243.0
14	1244.5
15	1299.0
16	1370.5
17	1605.0
18	1890.0
19	2216.0
20	2827.5
21	3768.0
22	4987.0
23	6737.5
24	9597.5
25	12635.5
26	16231.0
27	20842.0
28	26475.0
29	33533.5
30	39515.5
31	44459.5
32	48269.0
33	51623.0
34	55239.0
35	59842.5
36	65300.0
37	70336.0
38	76445.0
39	80762.0
40	85137.5
41	89089.0
42	89817.5
43	92060.5
44	94005.5
45	94133.5
46	96690.0
47	96592.0
48	95858.5
49	99486.0
50	102372.5
51	99871.0
52	95472.0
53	92048.0
54	89676.5
55	89138.5
56	80688.5
57	69285.5
58	63514.0
59	60275.0
60	54237.0
61	44290.0
62	36604.0
63	29936.0
64	23463.5
65	19296.5
66	15908.5
67	12496.0
68	9620.5
69	7267.5
70	5316.0
71	4070.5
72	3253.5
73	2621.0
74	2216.5
75	1768.0
76	1209.0
77	874.5
78	718.0
79	544.0
80	364.0
81	228.5
82	161.0
83	131.0
84	104.5
85	87.0
86	78.5
87	67.0
88	55.5
89	43.0
90	40.5
91	37.0
92	27.5
93	20.5
94	21.5
95	18.0
96	15.5
97	18.0
98	15.5
99	16.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.085843960204288E-4
2	1.3562250560544768E-4
3	1.0171687920408576E-4
4	1.695281320068096E-4
5	1.0171687920408576E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0171687920408576E-4
84-85	6.272540884251955E-4
86-87	0.0011866969240476673
88-89	0.0029667423101191683
90-91	0.002576827606503506
92-93	0.0018309038256735437
94-95	0.0019495735180783105
96-97	0.0018139510124728629
98-99	0.002254724155690568
100	0.002542921980102144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2949363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.438195906197066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.21966853897605	29.375578540190904
2	14.846468503168204	12.60114677705273
3	6.065791758980403	7.722637769814181
4	3.091648600019702	5.24815955843024
5	1.820778721919512	3.8635282051327677
6	1.1535140799750516	2.937183390392274
7	0.7738878021004936	2.298968150846991
8	0.5239894072923209	1.778973209555488
9	0.41577668651100713	1.5880331227845241
>10	1.8600961414609176	13.802445908440058
>50	0.12715126832311868	3.738049968387315
>100	0.09074977910070668	7.628858994107017
>500	0.00751907591011405	2.1550862348822166
>1k	0.002799655923946032	2.1888628876735012
>5k	7.99901692556009E-5	0.29441699480136907
>10k+	7.99901692556009E-5	2.7780702875083176
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81837	2.7747347478082554	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8673	0.2940634977790119	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4180	0.14172551835769281	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3851	0.13057056727164476	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3456	0.1171778448431068	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3386	0.11480445099501146	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3169	0.10744693006591592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.390562640136192E-5	0.0
3	0.0	0.0	0.0	3.390562640136192E-5	0.0
4	0.0	0.0	0.0	3.390562640136192E-5	0.0
5	0.0	0.0	0.0	3.390562640136192E-5	0.0
6	0.0	0.0	0.0	3.390562640136192E-5	0.0
7	0.0	0.0	0.0	3.390562640136192E-5	0.0
8	0.0	0.0	0.0	3.390562640136192E-5	0.0
9	0.0	0.0	0.0	6.781125280272384E-5	0.0
10-11	0.0	0.0	0.0	6.781125280272384E-5	0.0
12-13	0.0	0.0	0.0	6.781125280272384E-5	0.0
14-15	0.0	0.0	0.0	8.476406600340481E-5	0.0
16-17	0.0	0.0	0.0	1.3562250560544768E-4	0.0
18-19	0.0	0.0	0.0	2.0343375840817154E-4	0.0
20-21	0.0	0.0	0.0	3.390562640136192E-4	0.0
22-23	0.0	0.0	0.0	9.493575392381338E-4	0.0
24-25	0.0	0.0	0.0	0.002407299474496696	0.0
26-27	0.0	0.0	0.0	0.003509232332540959	0.0
28-29	0.0	0.0	0.0	0.006374257763456041	0.0
30-31	0.0	0.0	0.0	0.012087355812085525	0.0
32-33	0.0	0.0	0.0	0.021750459336473675	0.0
34-35	0.0	0.0	0.0	0.034838031127399374	0.0
36-37	0.0	0.0	0.0	0.05246895685610757	0.0
38-39	0.0	0.0	0.0	0.07733873382150655	0.0
40-41	0.0	0.0	0.0	0.10307310426014024	0.0
42-43	0.0	0.0	0.0	0.12758687214832493	0.0
44-45	0.0	0.0	0.0	0.15644056021588393	0.0
46-47	0.0	0.0	0.0	0.19236357138812687	0.0
48-49	0.0	0.0	0.0	0.23138894737609444	0.0
50-51	0.0	0.0	0.0	0.272804670025358	0.0
52-53	0.0	0.0	0.0	0.3137457139050025	0.0
54-55	0.0	0.0	0.0	0.35963697923924587	0.0
56-57	0.0	0.0	0.0	0.41390293429462566	0.0
58-59	0.0	0.0	0.0	0.476797871269152	0.0
60-61	0.0	0.0	0.0	0.5463891694579474	0.0
62-63	0.0	0.0	0.0	0.6188963515172599	0.0
64-65	0.0	0.0	0.0	0.6950822940411201	0.0
66-67	0.0	0.0	0.0	0.7826605270358379	0.0
68-69	0.0	0.0	0.0	0.8845808399983319	0.0
70-71	0.0	0.0	0.0	0.99955481912535	0.0
72-73	0.0	0.0	0.0	1.1230899689187122	0.0
74-75	0.0	3.390562640136192E-5	0.0	1.2477270515701187	0.0
76-77	0.0	3.390562640136192E-5	0.0	1.3832308874831616	0.0
78-79	0.0	3.390562640136192E-5	0.0	1.5431128687787838	0.0
80-81	0.0	3.390562640136192E-5	0.0	1.7225075380683896	0.0
82-83	0.0	3.390562640136192E-5	0.0	1.9156678916769485	0.0
84-85	0.0	3.390562640136192E-5	0.0	2.113897136432511	0.0
86-87	0.0	3.390562640136192E-5	0.0	2.3283332706079243	0.0
88	0.0	3.390562640136192E-5	0.0	2.5047442447742108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4825	0.0	48.80195	1
GTATCAA	8610	0.0	37.610825	1
TCAACGC	9940	0.0	31.207163	4
ATCAACG	10050	0.0	31.005894	3
TATCAAC	11150	0.0	28.494995	2
CAACGCA	11515	0.0	27.183605	5
AACGCAG	11805	0.0	26.43619	6
ACGCAGA	11865	0.0	26.104443	7
GTACATG	17475	0.0	25.550722	1
CGCAGAG	12080	0.0	25.523115	8
TACATGG	17255	0.0	24.814196	2
ACATGGG	17790	0.0	23.116861	3
GCAGAGT	13170	0.0	22.875418	9
CATGGGG	10745	0.0	20.514605	4
GCCCATA	1730	0.0	20.375671	1
CAGAGTA	12750	0.0	19.997988	10-11
GTACGAC	145	6.603366E-4	19.448227	3
TATATCG	155	0.0010338643	18.193502	5
AGAGTAC	12595	0.0	17.893167	10-11
TTACGGT	185	1.6508193E-4	17.78374	4
>>END_MODULE