FastQCFastQC Report
Wed 25 May 2016
SRR2431349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949363
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444981.5087325636078028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315981.071349983030234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274640.9311841234870037No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174790.592636443869405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132700.4499276623460727No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87690.2973184379135427No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.2677866373179565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.16644272000428567No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.12260274506732471No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.1039207449201743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95100.065.239711
GTATCAA203150.048.3788831
ATCAACG274600.035.7713133
TCAACGC276800.035.5719034
TATCAAC277550.035.3911132
CAACGCA290400.033.938375
AACGCAG297150.033.1516156
GTGGTAT28300.031.0580221
ACGCAGA324400.030.29447
CGCAGAG328050.029.9573338
TGGTATC30450.028.7087842
GCAGAGT345850.028.347569
TATAACG2750.025.6358532
GTACATG166700.025.2633531
TACATGG165650.024.5138472
ACATGGG168000.023.6394083
GAGTACT257250.023.58631112-13
AGAGTAC338150.023.32231910-11
CAGAGTA341250.023.1724310-11
AGTACTT259650.023.0153312-13
Produced by FastQC (version 0.11.5)