##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431349_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2949363
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69296115805345	34.0	31.0	34.0	31.0	34.0
2	32.81684994353018	34.0	31.0	34.0	31.0	34.0
3	32.89230182924245	34.0	31.0	34.0	31.0	34.0
4	36.30179872738622	37.0	37.0	37.0	35.0	37.0
5	36.26035689740463	37.0	37.0	37.0	35.0	37.0
6	36.29795823708373	37.0	37.0	37.0	35.0	37.0
7	36.167474129159416	37.0	37.0	37.0	35.0	37.0
8	36.21902932938401	37.0	37.0	37.0	35.0	37.0
9	38.017954046348315	39.0	39.0	39.0	35.0	39.0
10-11	38.01261916556219	39.0	39.0	39.0	35.0	39.0
12-13	37.980653449575385	39.0	39.0	39.0	35.0	39.0
14-15	39.39698758681112	41.0	39.0	41.0	36.0	41.0
16-17	39.412717763123766	41.0	39.0	41.0	36.0	41.0
18-19	39.495666182833375	41.0	39.5	41.0	36.0	41.0
20-21	39.4812981650614	41.0	40.0	41.0	36.0	41.0
22-23	39.37528001809204	41.0	39.0	41.0	35.5	41.0
24-25	39.313334947241145	41.0	39.0	41.0	35.0	41.0
26-27	39.18760033946313	41.0	39.0	41.0	35.0	41.0
28-29	39.07210014501436	41.0	39.0	41.0	35.0	41.0
30-31	38.821123917266206	41.0	38.5	41.0	35.0	41.0
32-33	38.53124081369435	40.5	38.0	41.0	35.0	41.0
34-35	38.38769676706462	40.0	38.0	41.0	35.0	41.0
36-37	38.25420811205674	40.0	38.0	41.0	35.0	41.0
38-39	38.12042193517719	40.0	37.0	41.0	34.0	41.0
40-41	37.99255924075809	40.0	37.0	41.0	34.0	41.0
42-43	37.803206488994405	40.0	37.0	41.0	33.5	41.0
44-45	37.527344209580164	40.0	37.0	41.0	33.0	41.0
46-47	37.123318323312525	40.0	36.0	41.0	32.5	41.0
48-49	36.77985992229509	40.0	36.0	41.0	31.0	41.0
50-51	36.59251082352359	40.0	35.0	41.0	31.0	41.0
52-53	36.45533866126347	40.0	35.0	41.0	31.0	41.0
54-55	36.28213515935475	39.5	35.0	41.0	31.0	41.0
56-57	36.07004359924499	39.0	35.0	41.0	31.0	41.0
58-59	35.84281029496878	39.0	35.0	41.0	30.0	41.0
60-61	35.612000286163486	38.0	35.0	41.0	30.0	41.0
62-63	35.36273595349233	37.5	35.0	41.0	30.0	41.0
64-65	35.083349014685545	37.0	35.0	40.0	29.0	41.0
66-67	34.79664303783562	36.5	35.0	40.0	29.0	41.0
68-69	34.53472851595412	36.0	35.0	39.5	29.0	41.0
70-71	34.29419233915934	35.5	35.0	39.0	29.0	41.0
72-73	34.05777620455672	35.0	35.0	39.0	29.0	41.0
74-75	33.78952912883223	35.0	35.0	37.5	29.0	40.5
76-77	33.51398047646221	35.0	34.5	37.0	29.0	39.5
78-79	33.242268076191365	35.0	34.0	37.0	29.0	39.0
80-81	32.98842173716833	35.0	34.0	36.0	28.0	39.0
82-83	32.727855302992545	35.0	34.0	36.0	27.5	37.5
84-85	32.51135652003501	35.0	34.0	35.5	27.0	37.0
86-87	32.33072361726922	35.0	34.0	35.0	27.0	37.0
88-89	32.143488271874304	35.0	34.0	35.0	27.0	36.0
90-91	31.969363384568126	35.0	34.0	35.0	26.0	36.0
92-93	31.836257354554185	35.0	34.0	35.0	26.0	36.0
94-95	31.713998582066704	35.0	34.0	35.0	25.0	36.0
96-97	31.586534787342217	35.0	34.0	35.0	25.0	35.5
98-99	31.473093173000407	35.0	34.0	35.0	25.0	35.0
100	31.368668420943777	35.0	34.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20444477886395163
1101	2	0.1987487428211665
1101	3	0.23424971014473783
1101	4	0.17388675378793295
1101	5	0.18419330519009947
1101	6	0.1476009816302053
1101	7	0.1340369777876731
1101	8	0.1610708653385302
1101	9	0.23155567336494443
1101	10-11	0.24096890683217964
1101	12-13	0.20098628398345397
1101	14-15	0.3068591801384528
1101	16-17	0.3195096028501965
1101	18-19	0.31034551757073103
1101	20-21	0.33047061739029715
1101	22-23	0.3141287857701869
1101	24-25	0.28143298363821856
1101	26-27	0.21764803780796171
1101	28-29	0.21798031339608315
1101	30-31	0.2493976600532335
1101	32-33	0.2505133833896167
1101	34-35	0.2919964618020714
1101	36-37	0.2608222103571691
1101	38-39	0.21035228453098398
1101	40-41	0.2050837342010965
1101	42-43	0.18284903298334854
1101	44-45	0.2740607022492654
1101	46-47	0.2506627868837583
1101	48-49	0.2559603749270778
1101	50-51	0.202366798222549
1101	52-53	0.17930906570317262
1101	54-55	0.14526250210386138
1101	56-57	0.08705804196245026
1101	58-59	0.15557180471272147
1101	60-61	0.1389285128766602
1101	62-63	0.1024469447077152
1101	64-65	0.10759479732815436
1101	66-67	0.08202327088979189
1101	68-69	0.049364537096565186
1101	70-71	0.06561901223511768
1101	72-73	0.06727387705614518
1101	74-75	0.06537359049382019
1101	76-77	0.05665511591125494
1101	78-79	0.07665155622915165
1101	80-81	0.07852197679795125
1101	82-83	0.13937139079534688
1101	84-85	0.1135668100925713
1101	86-87	0.06367677023010998
1101	88-89	0.08707468248733363
1101	90-91	0.0986609518145265
1101	92-93	0.08193817291355998
1101	94-95	0.06112009185693523
1101	96-97	0.08792657746812438
1101	98-99	0.09535856886521898
1101	100	0.1495232476853623
1102	1	0.07013323224096268
1102	2	0.12389189397278244
1102	3	0.1351476243141292
1102	4	0.06022765917287387
1102	5	0.09204824919747523
1102	6	0.07736588839598113
1102	7	0.13181567354023116
1102	8	0.15218096711750206
1102	9	0.15206026448250043
1102	10-11	0.20250067891886658
1102	12-13	0.19203748026910006
1102	14-15	0.26800171984924503
1102	16-17	0.2934620018101697
1102	18-19	0.24460596675407942
1102	20-21	0.2168455817334234
1102	22-23	0.20459211543515465
1102	24-25	0.21265138270153727
1102	26-27	0.2652799259471763
1102	28-29	0.25629661134876613
1102	30-31	0.21851341687960257
1102	32-33	0.22237618274310478
1102	34-35	0.23295101166777954
1102	36-37	0.17782959163859857
1102	38-39	0.1809570930077129
1102	40-41	0.17642904744538868
1102	42-43	0.20729667320167522
1102	44-45	0.2581416952320481
1102	46-47	0.3287285897512291
1102	48-49	0.4441717982956561
1102	50-51	0.4817930329108009
1102	52-53	0.3980030465940203
1102	54-55	0.418209372589736
1102	56-57	0.384526672161563
1102	58-59	0.36205866488668903
1102	60-61	0.3223399637564768
1102	62-63	0.3388790110698068
1102	64-65	0.3149389229800832
1102	66-67	0.39172401838039406
1102	68-69	0.3666326149464041
1102	70-71	0.38907165674967814
1102	72-73	0.3259461730434339
1102	74-75	0.2746154152785749
1102	76-77	0.30920979284238115
1102	78-79	0.2802195786904207
1102	80-81	0.3079392481286547
1102	82-83	0.3714195441006325
1102	84-85	0.32602857178513034
1102	86-87	0.3442844756687933
1102	88-89	0.3172596457363639
1102	90-91	0.37489254624849266
1102	92-93	0.40153969450577165
1102	94-95	0.4014365418896304
1102	96-97	0.3779524100626155
1102	98-99	0.41466844374851064
1102	100	0.47162112586928373
1103	1	0.13216645012875716
1103	2	0.12487102958930052
1103	3	0.10911302982100324
1103	4	0.14297748563438262
1103	5	0.14045602386504896
1103	6	0.11340517227939273
1103	7	0.10327827968291814
1103	8	0.12181617984111881
1103	9	0.13293126588764892
1103	10-11	0.15077596965330997
1103	12-13	0.16703026467429538
1103	14-15	0.21175479998574787
1103	16-17	0.25515716445391945
1103	18-19	0.24104990549322736
1103	20-21	0.2113251591222678
1103	22-23	0.24205272521351873
1103	24-25	0.26909700756727517
1103	26-27	0.2902146273517161
1103	28-29	0.23417479777872785
1103	30-31	0.2814018185856426
1103	32-33	0.24653899163892845
1103	34-35	0.24897055760057185
1103	36-37	0.22431820317903117
1103	38-39	0.2454320896730664
1103	40-41	0.30637352109984306
1103	42-43	0.3201357389872399
1103	44-45	0.31015628213760493
1103	46-47	0.17984014959385064
1103	48-49	0.18580254421680564
1103	50-51	0.16273988015859686
1103	52-53	0.2209922361582386
1103	54-55	0.246793737969071
1103	56-57	0.2698132832298441
1103	58-59	0.2221709702313106
1103	60-61	0.19981008844744252
1103	62-63	0.1867292534146756
1103	64-65	0.23219461941902608
1103	66-67	0.30412054758674856
1103	68-69	0.2583614654917028
1103	70-71	0.23534294663532052
1103	72-73	0.19421317524769677
1103	74-75	0.25388647720276936
1103	76-77	0.20299659551052684
1103	78-79	0.09125709226033507
1103	80-81	0.12259339574330141
1103	82-83	0.1738022547115179
1103	84-85	0.16620407224647948
1103	86-87	0.13659855065819926
1103	88-89	0.10991261783896533
1103	90-91	0.1152432928192546
1103	92-93	0.1429774971532538
1103	94-95	0.10782739152427112
1103	96-97	0.0904346606546973
1103	98-99	0.13487699324617708
1103	100	0.20997983688044286
1104	1	0.04383735396772437
1104	2	0.1156293911412476
1104	3	0.14202664127265052
1104	4	0.04407742145179583
1104	5	0.07179654275101655
1104	6	0.035364565986419905
1104	7	0.01826976211378195
1104	8	0.07400085279613222
1104	9	0.10557317494463803
1104	10-11	0.09447519981272023
1104	12-13	0.1531750500317841
1104	14-15	0.2321408908455922
1104	16-17	0.2508761250931997
1104	18-19	0.20639311878619537
1104	20-21	0.16251771225982736
1104	22-23	0.19093095516662828
1104	24-25	0.2130214725819215
1104	26-27	0.20986863483820173
1104	28-29	0.20295979358594707
1104	30-31	0.1335717601530888
1104	32-33	0.12428179259323713
1104	34-35	0.2272211416574521
1104	36-37	0.2595761613409664
1104	38-39	0.20909807432672523
1104	40-41	0.185121350819486
1104	42-43	0.19307652815503928
1104	44-45	0.13084030684147052
1104	46-47	0.10729431581951587
1104	48-49	0.09451537141190158
1104	50-51	0.0812108205398232
1104	52-53	0.11098632958444199
1104	54-55	0.027996534152322283
1104	56-57	0.04518322096549099
1104	58-59	-0.05255901079428327
1104	60-61	-0.09312098337841235
1104	62-63	-0.13386148807762055
1104	64-65	0.009574848331467933
1104	66-67	0.009413239580190691
1104	68-69	0.00898016040525107
1104	70-71	-0.03933656682935549
1104	72-73	-0.04453997693045153
1104	74-75	-0.03213217429933479
1104	76-77	-0.08195937504726203
1104	78-79	-0.066070096647465
1104	80-81	-0.08018958650169594
1104	82-83	0.023045603449141083
1104	84-85	0.0047956114066280975
1104	86-87	0.025937734022967618
1104	88-89	0.04612448299619132
1104	90-91	0.05316684941780281
1104	92-93	-0.023205054762328814
1104	94-95	0.045742945743370456
1104	96-97	0.06460134426070852
1104	98-99	0.022355068478827178
1104	100	0.0047841080248893775
1105	1	0.004041367577940491
1105	2	0.05285235269496269
1105	3	0.08321750362402014
1105	4	-0.043826300951394614
1105	5	-0.057635156223447837
1105	6	-0.05131300625604496
1105	7	-0.4136246611163301
1105	8	-0.3475668952722657
1105	9	-0.27225156870627387
1105	10-11	-0.29495585580955463
1105	12-13	-0.12883425170073792
1105	14-15	-0.16863090492022792
1105	16-17	-0.11691034666451827
1105	18-19	-0.11534899750279237
1105	20-21	-0.1959744726398327
1105	22-23	-0.18077880155433945
1105	24-25	-0.16825285453625582
1105	26-27	-0.15034253940984144
1105	28-29	-0.21824079001476093
1105	30-31	-0.14310391344421447
1105	32-33	-0.22856968856521576
1105	34-35	-0.24203049199756776
1105	36-37	-0.2153723966601504
1105	38-39	-0.22688050099835522
1105	40-41	-0.23446404650516683
1105	42-43	-0.1747194251946027
1105	44-45	-0.2576659445524072
1105	46-47	-0.11019438159938488
1105	48-49	-0.15855538464791863
1105	50-51	-0.14084431043819734
1105	52-53	-0.14300268666026028
1105	54-55	-0.10440211780962727
1105	56-57	-0.09403999583501133
1105	58-59	-0.15017528623185683
1105	60-61	-0.07878769576612399
1105	62-63	-0.1141966664240428
1105	64-65	-0.008781717228849573
1105	66-67	0.0264503805463292
1105	68-69	-0.05014559261056917
1105	70-71	-0.028162307152634014
1105	72-73	-0.024730953642617237
1105	74-75	-0.04856982271096655
1105	76-77	-0.027013317161618033
1105	78-79	-0.028361834644201167
1105	80-81	-0.09003037370417388
1105	82-83	-0.09602542042202344
1105	84-85	-0.04726938042698947
1105	86-87	-0.08223181498946985
1105	88-89	-0.07800008394809055
1105	90-91	-0.07256912279961725
1105	92-93	-0.04821348058190367
1105	94-95	-0.06661083560274506
1105	96-97	-0.07108963053186912
1105	98-99	-0.08652737409094158
1105	100	-0.12500614636447693
1106	1	0.09077638508942698
1106	2	0.05815543918381394
1106	3	0.0699624706612525
1106	4	0.06215858027734811
1106	5	0.10099344923191467
1106	6	0.09647453369635173
1106	7	0.09830543203462838
1106	8	0.0692868929586794
1106	9	0.10485461548746144
1106	10-11	0.09141020280549128
1106	12-13	0.08013584144163133
1106	14-15	0.1423367460267997
1106	16-17	0.1168507822878837
1106	18-19	0.07518458150261154
1106	20-21	0.050500744186308566
1106	22-23	0.0940816233430013
1106	24-25	0.09359757264399349
1106	26-27	0.04852140613845535
1106	28-29	0.0890375939590129
1106	30-31	0.09004116598938339
1106	32-33	0.057985346640762714
1106	34-35	0.043663082787603
1106	36-37	0.07136692479937068
1106	38-39	0.07392618336566414
1106	40-41	0.10725289818115868
1106	42-43	0.143427688686252
1106	44-45	0.06084810504732019
1106	46-47	0.08699684689013054
1106	48-49	0.14489079512595282
1106	50-51	0.1446218044501677
1106	52-53	0.15141526447371945
1106	54-55	0.1410829494763277
1106	56-57	0.1427830540498931
1106	58-59	0.14253082592173172
1106	60-61	0.13087870318280892
1106	62-63	0.18790235615043827
1106	64-65	0.1491278015478983
1106	66-67	0.15408060529505718
1106	68-69	0.16830582434894126
1106	70-71	0.19044726111415144
1106	72-73	0.12249892451486488
1106	74-75	0.10530045157288725
1106	76-77	0.15779029562173008
1106	78-79	0.1373862897306708
1106	80-81	0.1300038979578062
1106	82-83	0.21115287797724136
1106	84-85	0.2109383611626825
1106	86-87	0.23933462997199229
1106	88-89	0.19512738471760827
1106	90-91	0.2216756507746105
1106	92-93	0.21015997910647144
1106	94-95	0.19147760827533133
1106	96-97	0.156693760028233
1106	98-99	0.21459883279887393
1106	100	0.1752511674760875
1107	1	-0.042468367088837056
1107	2	0.00634011721874117
1107	3	0.08418111338813361
1107	4	-0.08081476237627072
1107	5	-0.05287005007074441
1107	6	-0.02431304376818133
1107	7	-0.011571070898433788
1107	8	-0.016584180920311553
1107	9	-1.4658153356350567E-4
1107	10-11	-0.035894638388910494
1107	12-13	0.05125539433628745
1107	14-15	0.032992785513620504
1107	16-17	0.06489251346334868
1107	18-19	0.06611643958149216
1107	20-21	-0.014669566237941467
1107	22-23	0.055096879483912176
1107	24-25	0.017360095187093805
1107	26-27	0.007143939591472304
1107	28-29	-0.008529694694814793
1107	30-31	0.05781923109485376
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2216	50-51	-0.6666019570959634
2216	52-53	-0.6712882016044333
2216	54-55	-0.747811247394381
2216	56-57	-0.7414069186747057
2216	58-59	-0.7555771572728318
2216	60-61	-0.6673661612001354
2216	62-63	-0.7321436031663069
2216	64-65	-0.6945621504728123
2216	66-67	-0.6516207774185929
2216	68-69	-0.6578777745547555
2216	70-71	-0.7587180293572118
2216	72-73	-0.7518688941610918
2216	74-75	-0.6926833620395954
2216	76-77	-0.5735722827813134
2216	78-79	-0.5648038960662376
2216	80-81	-0.5815720486415472
2216	82-83	-0.6575382952007018
2216	84-85	-0.6372941098173719
2216	86-87	-0.5697613632354788
2216	88-89	-0.5290229481272917
2216	90-91	-0.5436042909470551
2216	92-93	-0.6610808712024614
2216	94-95	-0.6982269741595601
2216	96-97	-0.7455467556298991
2216	98-99	-0.7410697708311744
2216	100	-0.7723329206674165
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	518.0
4	405.0
5	562.0
6	747.0
7	1320.0
8	2159.0
9	3261.0
10	3543.0
11	4235.0
12	5985.0
13	8410.0
14	8705.0
15	11319.0
16	15070.0
17	23618.0
18	28657.0
19	15103.0
20	9480.0
21	8553.0
22	8546.0
23	8607.0
24	9598.0
25	11438.0
26	14148.0
27	17939.0
28	22306.0
29	28037.0
30	35340.0
31	43658.0
32	55144.0
33	69856.0
34	100429.0
35	201654.0
36	312037.0
37	635323.0
38	837782.0
39	382060.0
40	3771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98594959268874	20.84906696831843	14.951734361987537	23.213249077005294
2	14.227755019560162	22.239419915222346	39.19461225851557	24.33821280670192
3	15.632973174025539	29.417738119981294	31.16924684582295	23.780041860170215
4	10.69310146767328	19.06805493111233	38.45011721449269	31.788726386721706
5	12.002049256059697	38.453862749346214	34.51182509579187	15.032262898802214
6	27.512551015253123	39.452349541239926	18.382104881630372	14.65299456187658
7	24.387401618586793	34.655720574239254	23.252512491680406	17.704365315493547
8	25.78044818491315	33.46119145049287	20.649645364100657	20.108715000493326
9	26.720040903747687	15.306050831993215	21.768564940972	36.205343323287096
10-11	24.031494258251698	26.816231165848354	28.07633377105497	21.07594080484498
12-13	25.648402722893042	25.62183766460758	28.505222992218997	20.22453662028038
14-15	22.89836822391818	25.701888848541195	28.26010226615035	23.139640661390274
16-17	20.352581218385122	28.72613510103707	28.518124083064716	22.40315959751309
18-19	20.21555162928402	25.90642453980741	33.34808228081793	20.529941550090648
20-21	21.789010033692023	24.869217522563346	33.30842965074153	20.0333427930031
22-23	21.413316308176586	25.128104019819887	33.28110733138466	20.177472340618866
24-25	20.82272792155855	25.152821341857674	33.406496123394945	20.617954613188832
26-27	20.700491007242114	25.5404292240431	33.35543428194493	20.40364548676986
28-29	20.5404947216915	25.25325200426537	33.932208446988895	20.274044827054237
30-31	20.917278801265585	25.27875583123581	33.44368115869794	20.360284208800667
32-33	20.504734535261452	25.373321576606262	33.53593399776586	20.58600989036643
34-35	21.10844768192654	25.096504804429532	33.25424423628669	20.54080327735724
36-37	20.966623005804532	25.290065565888515	32.96756765370957	20.77574377459738
38-39	20.89717570864973	25.235984882455547	32.958321981725426	20.908517427169297
40-41	20.873456229198258	25.234295107402776	32.986225075205	20.906023588193968
42-43	21.045142858692838	25.403953687391244	32.90835805066069	20.642545403255227
44-45	21.092918778713955	25.313950531473033	32.47991930425348	21.11321138555953
46-47	21.30256292886781	25.328772871183897	32.052148630377935	21.316515569570356
48-49	21.284449770012756	25.511766544806996	31.44518654294892	21.758597142231327
50-51	21.479466713321283	25.822872493543453	30.98237804954544	21.71528274358982
52-53	21.41523803872871	25.95564295674065	30.920749823054983	21.70836918147566
54-55	21.500399664671594	26.08714300366437	30.862029652321404	21.550427679342636
56-57	21.553622429699935	25.92021027080809	30.823935325180436	21.702231974311537
58-59	21.80260362894134	25.397130549325357	30.99689931154205	21.803366510191246
60-61	21.69431368603323	25.73814905537773	30.987079032743956	21.580458225845085
62-63	21.35435685603976	25.578167217802623	31.362907855018186	21.70456807113943
64-65	21.65199739740412	25.667847599634225	31.11012445738283	21.570030545578824
66-67	21.64922604744585	25.55948939101989	31.09045376577344	21.700830795760815
68-69	21.797519277601705	25.6484453738418	30.931080401818374	21.62295494673813
70-71	21.7629422549908	25.7075359343438	30.89850905425944	21.631012756405966
72-73	21.71022439941662	25.674150150803825	30.808600437755768	21.80702501202379
74-75	21.798359579351676	25.6534379546093	30.67823278335621	21.869969682682814
76-77	21.794348211359825	25.53734306497077	30.556212480073995	22.11209624359541
78-79	21.667368239744388	25.623189309958942	30.78467628773979	21.92476616255688
80-81	21.242683309511584	25.915261251220826	30.938799604182027	21.903255835085563
82-83	21.436002909817855	25.947068236449315	30.876161346043357	21.74076750768947
84-85	21.396814762159945	25.56531165017617	30.73356288872249	22.3043106989414
86-87	21.57876293906444	25.724417232454506	30.619286565682952	22.077533262798102
88-89	21.234577361470468	25.674249326199934	30.68152728241827	22.409646029911332
90-91	21.27562508752056	25.69579904703037	30.488619869006516	22.539955996442554
92-93	21.175266306477123	25.80742998165146	30.81961854764238	22.19768516422904
94-95	21.202028527825462	25.520719783571256	30.852403477263145	22.42484821134013
96-97	21.31102017400114	25.975141144091918	30.384336438825464	22.32950224308147
98-99	20.8189760764658	25.877392628910158	30.794334573461263	22.509296721162787
100	21.144529618236955	25.788671343087632	30.5390090544624	22.52778998421301
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11546.0
1	7246.0
2	9660.0
3	12812.5
4	5647.5
5	2950.0
6	7238.0
7	19520.0
8	32559.5
9	32157.0
10	17656.0
11	5692.0
12	3390.0
13	3192.0
14	3370.0
15	3538.0
16	3823.5
17	4248.5
18	4652.0
19	4961.0
20	5498.0
21	6410.5
22	7510.0
23	9062.0
24	11681.0
25	14557.0
26	17882.0
27	22128.0
28	27586.0
29	34557.0
30	40645.0
31	45682.5
32	49285.0
33	52314.0
34	55635.0
35	60057.5
36	65008.5
37	69681.5
38	75641.5
39	80094.0
40	84647.0
41	88526.0
42	89586.5
43	92211.0
44	95067.0
45	95489.5
46	96612.0
47	97073.0
48	94956.0
49	96658.5
50	101969.0
51	100000.5
52	93798.0
53	89854.0
54	87093.0
55	84730.0
56	75847.5
57	65415.0
58	61423.0
59	59844.5
60	53263.0
61	42528.5
62	34772.0
63	28402.5
64	22103.5
65	17837.0
66	14555.5
67	11186.0
68	8422.5
69	6180.5
70	4366.5
71	3351.0
72	2631.5
73	2030.0
74	1831.0
75	1444.0
76	908.0
77	651.5
78	478.0
79	297.0
80	134.0
81	53.0
82	36.5
83	23.0
84	8.0
85	6.5
86	6.5
87	4.5
88	4.0
89	5.0
90	2.0
91	0.5
92	1.0
93	2.5
94	4.5
95	6.5
96	9.0
97	8.5
98	3.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009052802249163633
2	3.390562640136192E-5
3	6.781125280272384E-4
4	0.0023394882216939725
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.781125280272384E-5
24-25	1.3562250560544768E-4
26-27	5.933484620238336E-4
28-29	9.493575392381338E-4
30-31	0.0017800453860715008
32-33	0.004390778618976369
34-35	0.005340136158214502
36-37	0.003933052662557983
38-39	0.002525969166901463
40-41	0.0030854120025239347
42-43	5.085843960204288E-4
44-45	4.40773143217705E-4
46-47	0.0034075154533368727
48-49	0.0023394882216939725
50-51	0.0010171687920408575
52-53	1.695281320068096E-5
54-55	5.255372092211098E-4
56-57	7.967822204320051E-4
58-59	6.103012752245146E-4
60-61	3.2210345081293827E-4
62-63	0.0
64-65	0.0
66-67	7.459237808299622E-4
68-69	6.950653412279194E-4
70-71	0.0020512903972823965
72-73	5.085843960204288E-5
74-75	0.0019834791444796724
76-77	4.577259564183859E-4
78-79	0.0013053666164524339
80-81	9.324047260374529E-4
82-83	0.001271460990051072
84-85	0.0013053666164524339
86-87	3.2210345081293827E-4
88-89	0.0015596588144626484
90-91	0.0018987150784762676
92-93	0.0027972141781123583
94-95	0.0010849800448435815
96-97	0.0024412051008980582
98-99	0.0023903466612960156
100	0.00335665701373483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2949363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.50374232325308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.45857773942242	25.204002101253515
2	16.61217525442382	12.792618308501119
3	6.781655615883937	7.833573610771118
4	3.4610013303327394	5.330460136142716
5	2.0234444865768064	3.8955092558280233
6	1.2853531307246127	2.9694543443884265
7	0.8966641501977195	2.4167447772798183
8	0.6150100786982512	1.8944151677120844
9	0.4580065404617912	1.587146923467485
>10	2.14047286069345	14.460529938798794
>50	0.14426441684075406	3.8395608669500065
>100	0.1100098753958135	8.312817784985777
>500	0.009759616833065705	2.488623246727193
>1k	0.0029894321830873146	1.8633697744879854
>5k	1.758489519463126E-4	0.5642481792008004
>10k+	4.3962237986578154E-4	4.546925583505148
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44498	1.5087325636078028	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31598	1.071349983030234	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27464	0.9311841234870037	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17479	0.592636443869405	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13270	0.4499276623460727	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8769	0.2973184379135427	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7898	0.2677866373179565	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4909	0.16644272000428567	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3616	0.12260274506732471	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3065	0.1039207449201743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.390562640136192E-5	0.0
10-11	0.0	0.0	0.0	3.390562640136192E-5	0.0
12-13	0.0	0.0	0.0	3.390562640136192E-5	0.0
14-15	0.0	0.0	0.0	5.0858439602042885E-5	0.0
16-17	0.0	0.0	0.0	1.0171687920408577E-4	0.0
18-19	0.0	0.0	0.0	1.3562250560544768E-4	0.0
20-21	0.0	0.0	0.0	2.7124501121089537E-4	0.0
22-23	0.0	0.0	0.0	8.815462864354099E-4	0.0
24-25	0.0	0.0	0.0	0.0023394882216939725	0.0
26-27	0.0	0.0	0.0	0.003441421079738235	0.0
28-29	0.0	0.0	0.0	0.006323399323853998	0.0
30-31	0.0	0.0	0.0	0.012121261438486887	0.0
32-33	0.0	0.0	0.0	0.02188608184207912	0.0
34-35	0.0	0.0	0.0	0.03478717268779733	0.0
36-37	0.0	0.0	0.0	0.052875824372923916	0.0
38-39	0.0	0.0	0.0	0.07801684634953378	0.0
40-41	0.0	0.0	0.0	0.10388683929377293	0.0
42-43	0.0	0.0	0.0	0.12858708812716507	0.0
44-45	0.0	0.0	0.0	0.1576611627663329	0.0
46-47	0.0	0.0	0.0	0.19409275833459633	0.0
48-49	0.0	0.0	0.0	0.23354195465258093	0.0
50-51	0.0	0.0	0.0	0.27514415824705196	0.0
52-53	0.0	0.0	0.0	0.31647511683031215	0.0
54-55	0.0	0.0	0.0	0.36328183407739234	0.0
56-57	0.0	0.0	0.0	0.4187514388700204	0.0
58-59	0.0	0.0	0.0	0.4821719130537679	0.0
60-61	0.0	0.0	0.0	0.5527125687818013	0.0
62-63	0.0	0.0	0.0	0.6258130993031377	0.0
64-65	0.0	0.0	0.0	0.7030670690586408	0.0
66-67	0.0	0.0	0.0	0.792221913681022	0.0
68-69	0.0	0.0	0.0	0.8957188382711792	0.0
70-71	0.0	0.0	0.0	1.0124898155974698	0.0
72-73	0.0	0.0	0.0	1.1383814064257265	0.0
74-75	0.0	0.0	0.0	1.2654257885516296	0.0
76-77	0.0	0.0	0.0	1.4029978676751558	0.0
78-79	0.0	0.0	0.0	1.5652362900056724	0.0
80-81	0.0	0.0	0.0	1.7474959847261933	0.0
82-83	0.0	0.0	0.0	1.9432162131280553	0.0
84-85	0.0	0.0	0.0	2.1451072655349646	0.0
86-87	0.0	0.0	0.0	2.364391904285773	0.0
88	0.0	0.0	0.0	2.5441086770261916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9510	0.0	65.23971	1
GTATCAA	20315	0.0	48.378883	1
ATCAACG	27460	0.0	35.771313	3
TCAACGC	27680	0.0	35.571903	4
TATCAAC	27755	0.0	35.391113	2
CAACGCA	29040	0.0	33.93837	5
AACGCAG	29715	0.0	33.151615	6
GTGGTAT	2830	0.0	31.058022	1
ACGCAGA	32440	0.0	30.2944	7
CGCAGAG	32805	0.0	29.957333	8
TGGTATC	3045	0.0	28.708784	2
GCAGAGT	34585	0.0	28.34756	9
TATAACG	275	0.0	25.635853	2
GTACATG	16670	0.0	25.263353	1
TACATGG	16565	0.0	24.513847	2
ACATGGG	16800	0.0	23.639408	3
GAGTACT	25725	0.0	23.586311	12-13
AGAGTAC	33815	0.0	23.322319	10-11
CAGAGTA	34125	0.0	23.17243	10-11
AGTACTT	25965	0.0	23.01533	12-13
>>END_MODULE