FastQCFastQC Report
Wed 25 May 2016
SRR2431348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4154963
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237350.5712445574124246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47500.047.098721
GTATCAA80400.036.9450951
TCAACGC94700.030.5225544
ATCAACG96600.029.9222153
CAACGCA98600.029.2199385
AACGCAG104850.027.5229956
TATCAAC110550.026.316472
ACGCAGA111200.025.6977187
CGCAGAG113500.025.0527428
GTACATG178200.023.7900091
TACATGG171550.023.5067332
ACATGGG178750.021.744793
GCAGAGT130000.021.2222069
CATGGGG110200.019.7040794
CAGAGTA128100.018.34495410-11
AGAGTAC118050.017.85632910-11
GTGGTAT24750.017.8504181
CATGGGT31550.016.088674
GAGTACA133600.015.9362991
CCCCTAT15350.015.615561
Produced by FastQC (version 0.11.5)