##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431348_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4154963
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23799923128076	33.0	31.0	34.0	28.0	34.0
2	31.36556522886004	34.0	31.0	34.0	28.0	34.0
3	31.422477408342747	34.0	31.0	34.0	28.0	34.0
4	34.65780609839366	37.0	35.0	37.0	32.0	37.0
5	34.76269872920649	37.0	35.0	37.0	32.0	37.0
6	34.81092852090379	37.0	35.0	37.0	32.0	37.0
7	34.73192878011188	37.0	35.0	37.0	32.0	37.0
8	34.712640521708614	37.0	35.0	37.0	32.0	37.0
9	36.23098785717225	39.0	38.0	39.0	32.0	39.0
10-11	36.24615766734866	39.0	37.5	39.0	32.0	39.0
12-13	36.19346838948987	39.0	37.0	39.0	32.0	39.0
14-15	37.59863986755117	41.0	38.0	41.0	32.0	41.0
16-17	37.611421449481014	41.0	38.0	41.0	32.0	41.0
18-19	37.64473871367808	41.0	38.5	41.0	32.0	41.0
20-21	37.689578583491596	41.0	39.0	41.0	32.0	41.0
22-23	37.695476229270874	41.0	38.5	41.0	32.0	41.0
24-25	37.71595487131896	41.0	38.0	41.0	31.5	41.0
26-27	37.70983086973338	41.0	38.0	41.0	31.0	41.0
28-29	37.67545162255355	41.0	38.0	41.0	31.0	41.0
30-31	37.60668662994111	40.0	38.0	41.0	31.0	41.0
32-33	37.52955670122694	40.0	38.0	41.0	30.5	41.0
34-35	37.46116620051731	40.0	38.0	41.0	30.0	41.0
36-37	37.36311478585971	40.0	38.0	41.0	30.0	41.0
38-39	37.2645074095726	40.0	38.0	41.0	30.0	41.0
40-41	37.17765838107343	40.0	38.0	41.0	30.0	41.0
42-43	37.05983531020613	40.0	38.0	41.0	30.0	41.0
44-45	36.92881212179266	40.0	37.0	41.0	30.0	41.0
46-47	36.80410740119707	40.0	37.0	41.0	30.0	41.0
48-49	36.67073545540598	40.0	37.0	41.0	30.0	41.0
50-51	36.53318501271852	40.0	36.0	41.0	30.0	41.0
52-53	36.36542106391802	40.0	36.0	41.0	29.0	41.0
54-55	36.20192394011691	40.0	35.5	41.0	29.0	41.0
56-57	35.997639329158886	39.5	35.0	41.0	29.0	41.0
58-59	35.77239159530422	39.0	35.0	41.0	28.0	41.0
60-61	35.59278157230281	39.0	35.0	41.0	28.0	41.0
62-63	35.43023259172224	39.0	35.0	41.0	28.0	41.0
64-65	35.183188755230795	38.0	35.0	40.5	28.0	41.0
66-67	34.88668791996463	38.0	34.5	40.0	28.0	41.0
68-69	34.58487945620695	37.0	34.0	40.0	27.0	41.0
70-71	34.33925452525089	37.0	34.0	40.0	27.0	41.0
72-73	34.103692018436746	36.0	34.0	39.0	27.0	41.0
74-75	33.76775785969694	36.0	34.0	39.0	26.5	41.0
76-77	33.421870664070894	35.0	34.0	38.5	26.0	40.0
78-79	33.09029538891201	35.0	34.0	37.0	26.0	39.5
80-81	32.74888921995214	35.0	33.0	37.0	26.0	39.0
82-83	32.41762730498442	35.0	33.0	36.5	25.5	39.0
84-85	32.12529172943297	35.0	33.0	36.0	25.0	38.0
86-87	31.85240626691501	35.0	33.0	36.0	25.0	37.0
88-89	31.60057454181902	35.0	33.0	35.5	24.0	37.0
90-91	31.38349980493208	35.0	33.0	35.0	24.0	36.5
92-93	31.176489898947352	35.0	32.0	35.0	23.0	36.0
94-95	30.958396982115126	35.0	32.0	35.0	21.0	36.0
96-97	30.76394675476051	35.0	32.0	35.0	20.0	36.0
98-99	30.64068849710575	35.0	32.0	35.0	18.5	35.5
100	30.59260359237856	35.0	32.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4253340225957558
1101	2	0.4731765704810904
1101	3	0.5099085153431915
1101	4	0.4779694046670713
1101	5	0.43654534967387804
1101	6	0.4284421043641444
1101	7	0.47592227172086154
1101	8	0.5356300736851267
1101	9	0.6215928352594418
1101	10-11	0.5769170700215511
1101	12-13	0.599440565604695
1101	14-15	0.7481331384208332
1101	16-17	0.6681096616187361
1101	18-19	0.6809296921389745
1101	20-21	0.6630401875533849
1101	22-23	0.6408397282163278
1101	24-25	0.682573284089429
1101	26-27	0.7369622746696365
1101	28-29	0.728790611835997
1101	30-31	0.7420500687069094
1101	32-33	0.7825176499852589
1101	34-35	0.7532581400448422
1101	36-37	0.7454753192215193
1101	38-39	0.7433911040186345
1101	40-41	0.7630080418795444
1101	42-43	0.798196213654137
1101	44-45	0.7870758868192098
1101	46-47	0.8278536553760105
1101	48-49	0.7808686484480489
1101	50-51	0.7779065335274851
1101	52-53	0.7607805875410136
1101	54-55	0.8079799273961754
1101	56-57	0.7592713525330836
1101	58-59	0.811431160264874
1101	60-61	0.8145917771366769
1101	62-63	0.8191977194206999
1101	64-65	0.8529096284858042
1101	66-67	0.7565185595739692
1101	68-69	0.8595982977470982
1101	70-71	0.8363552250084965
1101	72-73	0.8280599208618113
1101	74-75	0.8759947992987662
1101	76-77	0.8402787870253263
1101	78-79	0.7811775762497462
1101	80-81	0.812526782329634
1101	82-83	0.8313492319952331
1101	84-85	0.8565455755738114
1101	86-87	0.8956484656664117
1101	88-89	0.8976221698883045
1101	90-91	0.8250615762870517
1101	92-93	0.8261629407676914
1101	94-95	0.8792637725772856
1101	96-97	0.9013876458584811
1101	98-99	0.9245420093162906
1101	100	1.0377663392680745
1102	1	0.2617994153891523
1102	2	0.3494785927015762
1102	3	0.3873464513555902
1102	4	0.3326013586426484
1102	5	0.32391447187705324
1102	6	0.27738216310359576
1102	7	0.3205149475421578
1102	8	0.3084648565411712
1102	9	0.4146806386458479
1102	10-11	0.3799360595987338
1102	12-13	0.3794511377572647
1102	14-15	0.4624361230390619
1102	16-17	0.547856641088373
1102	18-19	0.47680987124925167
1102	20-21	0.4279939758685458
1102	22-23	0.4058587035708854
1102	24-25	0.4457535108455062
1102	26-27	0.46944983579730604
1102	28-29	0.499415198490901
1102	30-31	0.5355553061892167
1102	32-33	0.5044295028853796
1102	34-35	0.506515477142429
1102	36-37	0.5266798808920186
1102	38-39	0.4883009444170554
1102	40-41	0.4791194334924569
1102	42-43	0.4716634293141979
1102	44-45	0.4779108261241589
1102	46-47	0.47029231437193886
1102	48-49	0.4112564757758719
1102	50-51	0.4377257165323982
1102	52-53	0.46447325553986474
1102	54-55	0.46671131649642916
1102	56-57	0.535591627124596
1102	58-59	0.5218127296369204
1102	60-61	0.4705194124116403
1102	62-63	0.5104906273078598
1102	64-65	0.5233699438014412
1102	66-67	0.4602327985567669
1102	68-69	0.4241569340816369
1102	70-71	0.4407566539084016
1102	72-73	0.43137331881327867
1102	74-75	0.4485157361906005
1102	76-77	0.42773229681405667
1102	78-79	0.37924709170854953
1102	80-81	0.43288007237877224
1102	82-83	0.4188868923257161
1102	84-85	0.3826559478984777
1102	86-87	0.39676612708729664
1102	88-89	0.3484014513137872
1102	90-91	0.40516933638926034
1102	92-93	0.4356231209683763
1102	94-95	0.4022397150040895
1102	96-97	0.46131895057114747
1102	98-99	0.46071180851650695
1102	100	0.44348146479460837
1103	1	0.16572433695804278
1103	2	0.17504242148612903
1103	3	0.19987747596433536
1103	4	0.22648261012935933
1103	5	0.10180744651430018
1103	6	0.194254613251033
1103	7	0.14595041411187992
1103	8	0.22324581046093073
1103	9	0.27950625004943674
1103	10-11	0.2699695863730298
1103	12-13	0.306183242224364
1103	14-15	0.2812308646764379
1103	16-17	0.3124511107414776
1103	18-19	0.2963999200358671
1103	20-21	0.2526516392274232
1103	22-23	0.29297670255655817
1103	24-25	0.30781260234621755
1103	26-27	0.3538816534691023
1103	28-29	0.35196606089647986
1103	30-31	0.3473161575089705
1103	32-33	0.31920462215900614
1103	34-35	0.38854925525532735
1103	36-37	0.4061235249426929
1103	38-39	0.36847391923382844
1103	40-41	0.3566977860576799
1103	42-43	0.3041094476170372
1103	44-45	0.28802765369241
1103	46-47	0.22001157812936611
1103	48-49	0.2887877023531331
1103	50-51	0.20637302115029854
1103	52-53	0.2345540878296859
1103	54-55	0.24906420487067038
1103	56-57	0.10442204986714643
1103	58-59	0.12995475390007272
1103	60-61	0.15408052135345685
1103	62-63	0.1933731220230115
1103	64-65	0.23189166888189305
1103	66-67	0.23751941881555894
1103	68-69	0.2630125355663324
1103	70-71	0.23689501580906125
1103	72-73	0.20552942701454668
1103	74-75	0.14427035790356513
1103	76-77	0.18681841170272406
1103	78-79	0.18894292711767235
1103	80-81	0.1835185173109295
1103	82-83	0.12405010533069571
1103	84-85	0.18236735732457277
1103	86-87	0.1927393236206214
1103	88-89	0.20148326651708004
1103	90-91	0.2110412344894108
1103	92-93	0.2633520522856152
1103	94-95	0.1732792245347632
1103	96-97	0.24670989430271817
1103	98-99	0.20178322907331037
1103	100	0.09974091804338414
1104	1	0.25777529371273644
1104	2	0.27728574536487827
1104	3	0.27658596776337774
1104	4	0.24396084675569085
1104	5	0.23460291283083734
1104	6	0.2853305373488908
1104	7	0.272376716930367
1104	8	0.3029154421202449
1104	9	0.30912130335325116
1104	10-11	0.3238005257830423
1104	12-13	0.3146335054415772
1104	14-15	0.37231545011879064
1104	16-17	0.40240172859842005
1104	18-19	0.3589241305736337
1104	20-21	0.37500999229786913
1104	22-23	0.41914551236867226
1104	24-25	0.3941279662669501
1104	26-27	0.43134998139847625
1104	28-29	0.4417060283185421
1104	30-31	0.44804946585761485
1104	32-33	0.4707024865507563
1104	34-35	0.4675692117952792
1104	36-37	0.43734057042111374
1104	38-39	0.43175664367266364
1104	40-41	0.4653690903518708
1104	42-43	0.42924726030457094
1104	44-45	0.42152614485054585
1104	46-47	0.4086187278597677
1104	48-49	0.38294406407500503
1104	50-51	0.40342606628439626
1104	52-53	0.40332311883145167
1104	54-55	0.416188204392526
1104	56-57	0.4405280187279672
1104	58-59	0.3923441386513531
1104	60-61	0.39840932405584084
1104	62-63	0.36513042086967573
1104	64-65	0.4420628922229639
1104	66-67	0.49797499127353007
1104	68-69	0.5104199188291574
1104	70-71	0.5327493397795351
1104	72-73	0.4816338204912043
1104	74-75	0.5263805454779771
1104	76-77	0.5048707662171665
1104	78-79	0.4670899865258491
1104	80-81	0.46250970867902197
1104	82-83	0.435054145914755
1104	84-85	0.4179093226082955
1104	86-87	0.43458591572920824
1104	88-89	0.4721551399343973
1104	90-91	0.4677729933636421
1104	92-93	0.4247574251062822
1104	94-95	0.4463745361980145
1104	96-97	0.4483344356860144
1104	98-99	0.4310431791382072
1104	100	0.4415077366436009
1105	1	0.14529463850598034
1105	2	0.1629017517823499
1105	3	0.19041038569203295
1105	4	0.1770605653905335
1105	5	0.09217656275129116
1105	6	0.1061753839200037
1105	7	0.13282326581324355
1105	8	0.1777272369234808
1105	9	0.23938084130149662
1105	10-11	0.21886096746474948
1105	12-13	0.23956678449482638
1105	14-15	0.2744047779219656
1105	16-17	0.2630087893165012
1105	18-19	0.24254807770762454
1105	20-21	0.15664565071186587
1105	22-23	0.25104629697636227
1105	24-25	0.260289006882374
1105	26-27	0.24302995519981607
1105	28-29	0.3002463996364142
1105	30-31	0.2602579344530298
1105	32-33	0.28443544953764643
1105	34-35	0.287260686566313
1105	36-37	0.2709816578456099
1105	38-39	0.25505211614229495
1105	40-41	0.23436805832911745
1105	42-43	0.25680282353812345
1105	44-45	0.24276744138357742
1105	46-47	0.22989490586041228
1105	48-49	0.2717502826505154
1105	50-51	0.31351939899681724
1105	52-53	0.29430127209659673
1105	54-55	0.2994015506849834
1105	56-57	0.2516165248187008
1105	58-59	0.2860297667956786
1105	60-61	0.3626740566688156
1105	62-63	0.42359853557292126
1105	64-65	0.40609523194022046
1105	66-67	0.37822275184425536
1105	68-69	0.35060670443131414
1105	70-71	0.4212488958745553
1105	72-73	0.35748632085549303
1105	74-75	0.3101933963394288
1105	76-77	0.3564299017216115
1105	78-79	0.2903503521953965
1105	80-81	0.31311050515421357
1105	82-83	0.38810115177189175
1105	84-85	0.4137903500643034
1105	86-87	0.3758995590098735
1105	88-89	0.3924042524999827
1105	90-91	0.4018698833247818
1105	92-93	0.33915660609783416
1105	94-95	0.3121062283097267
1105	96-97	0.3534529380362308
1105	98-99	0.3280441090102322
1105	100	0.2543815219137677
1106	1	0.138722822638492
1106	2	0.15478330216311065
1106	3	0.1930645366083965
1106	4	0.17134405980858958
1106	5	0.21203571923307152
1106	6	0.14742495687099932
1106	7	0.19219002101222316
1106	8	0.20181313163247694
1106	9	0.25591116453627194
1106	10-11	0.18397136652138357
1106	12-13	0.195298966908112
1106	14-15	0.19993580647638964
1106	16-17	0.28055243097004023
1106	18-19	0.2669516417760036
1106	20-21	0.24358459096907836
1106	22-23	0.24823681042703072
1106	24-25	0.21885282729036248
1106	26-27	0.26018514756474076
1106	28-29	0.24638144079843016
1106	30-31	0.269125841306753
1106	32-33	0.29707155418945064
1106	34-35	0.277473660039675
1106	36-37	0.3345075397706836
1106	38-39	0.27599308419351587
1106	40-41	0.2572020106539128
1106	42-43	0.2777531866675389
1106	44-45	0.23464293042357554
1106	46-47	0.23735895108902838
1106	48-49	0.2985535866322664
1106	50-51	0.21542866623582313
1106	52-53	0.22856859375541916
1106	54-55	0.19335627246944398
1106	56-57	0.1899105329078381
1106	58-59	0.20115912131798552
1106	60-61	0.21692021873804634
1106	62-63	0.24793162277908465
1106	64-65	0.2336760406332843
1106	66-67	0.21496042803640592
1106	68-69	0.20609078514633694
1106	70-71	0.18632896067580162
1106	72-73	0.16622459767140896
1106	74-75	0.17992352891747743
1106	76-77	0.16545680856430778
1106	78-79	0.1432725796830283
1106	80-81	0.16167223164448075
1106	82-83	0.23770460232788082
1106	84-85	0.1541438915404143
1106	86-87	0.1418348789935564
1106	88-89	0.1894951395496598
1106	90-91	0.12204756326394417
1106	92-93	0.14634968184255115
1106	94-95	0.22214879545284916
1106	96-97	0.17443054964755333
1106	98-99	0.1612645544636777
1106	100	0.17915664617125415
1107	1	0.012135924855286362
1107	2	0.10370834048022104
1107	3	0.11930132623245626
1107	4	0.07921476329585886
1107	5	0.024358104947580728
1107	6	0.10606258532551038
1107	7	0.13448412600435944
1107	8	0.11248745165898555
1107	9	0.11646176356713767
1107	10-11	0.11769597032952106
1107	12-13	0.13968778366118784
1107	14-15	0.1643366208511381
1107	16-17	0.14834399448015034
1107	18-19	0.14159330847769525
1107	20-21	0.10393340660635886
1107	22-23	0.10464833028372311
1107	24-25	0.0976758213403599
1107	26-27	0.14007017330244764
1107	28-29	0.08955342415938361
1107	30-31	0.16762709867355596
1107	32-33	0.1596766468211328
1107	34-35	0.18466509695333855
1107	36-37	0.13693611480211132
1107	38-39	0.08606480530531968
1107	40-41	0.10840656843184604
1107	42-43	0.1017208313965483
1107	44-45	0.13284751915324478
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2216	84-85	-0.8637325242812111
2216	86-87	-0.9204242089504611
2216	88-89	-1.0025525387993888
2216	90-91	-0.9689275004850906
2216	92-93	-1.019445995083263
2216	94-95	-1.048746804381107
2216	96-97	-1.1530875372883393
2216	98-99	-1.198238499559924
2216	100	-1.1891734255834763
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71928.0
3	14905.0
4	10412.0
5	8972.0
6	6380.0
7	6262.0
8	6926.0
9	7371.0
10	7428.0
11	8232.0
12	9079.0
13	10301.0
14	10369.0
15	10261.0
16	10143.0
17	9858.0
18	9897.0
19	9961.0
20	9738.0
21	10345.0
22	12977.0
23	20471.0
24	33521.0
25	42829.0
26	41845.0
27	32098.0
28	32640.0
29	39087.0
30	47382.0
31	55984.0
32	72530.0
33	98227.0
34	142016.0
35	229854.0
36	403828.0
37	785437.0
38	1201555.0
39	606878.0
40	7036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.603037726816865	21.991413131665393	13.915694272059657	22.489854869458085
2	15.065078845524384	22.639375589656698	39.783463619385024	22.51208194543389
3	17.104993980691038	29.037618153908507	30.325235224774623	23.532152640625835
4	11.791906540616516	19.16088241523384	39.69198259429694	29.355228449852707
5	12.551646092466331	39.62364508355193	34.8651166641516	12.959592159830141
6	28.013895671273126	40.52945838506865	18.621922746363808	12.834723197294418
7	24.421757787012783	34.77397993676478	24.258940452658663	16.54532182356377
8	23.199701176641042	37.48822793367835	21.644524873025343	17.667546016655265
9	24.370878874252313	18.2721482718378	21.969846662894472	35.38712619101542
10-11	22.484051001176184	28.99058306897077	29.197648691456457	19.327717238396588
12-13	22.874066507932802	27.595311919745136	28.961701945360286	20.56891962696178
14-15	21.146638369583556	28.369434336719724	26.42169136042848	24.06223593326824
16-17	20.72008824145967	31.516875120187592	26.85767598893179	20.905360649420945
18-19	20.439411855171755	30.494976730238033	28.733288359005844	20.33232305558437
20-21	22.46869587045661	29.40381177882932	27.679199550032095	20.44829280068198
22-23	21.58028844059502	29.752346771800376	28.070045389092513	20.59731939851209
24-25	21.408229146685542	29.438420991955883	27.851138505926524	21.302211355432046
26-27	21.32455812482566	29.82280467960846	27.805734972850544	21.046902222715342
28-29	21.058587043976086	29.527784001927333	28.286136362706475	21.127492591390105
30-31	21.691829746739018	29.79061426058427	27.521015229257156	20.996540763419556
32-33	20.76437263099575	29.868148043676925	28.412274670075284	20.955204655252043
34-35	21.494716078097444	29.043399423773447	28.076495506698855	21.385388991430247
36-37	21.720518329525436	29.692358271301096	27.5932902410924	20.99383315808107
38-39	21.231512771593874	29.519504746492327	28.022475771745743	21.22650671016806
40-41	21.501755851977503	29.444606365929133	27.81071696667335	21.242920815420018
42-43	21.005686452562873	29.693296907818436	28.27872354097979	21.022293098638904
44-45	21.281380845027982	29.15909239143646	28.01101959271358	21.548507170821978
46-47	21.434775712804182	29.583584739503095	27.866722278874683	21.114917268818036
48-49	21.13115086704743	29.337264856510153	28.104413926188993	21.42717035025342
50-51	21.619037281439088	29.379094350539344	27.46318077922715	21.538687588794414
52-53	21.015566203597963	29.61510126564304	28.085220012789524	21.284112517969476
54-55	21.413993337606136	29.29899736772626	27.810307817422203	21.476701477245406
56-57	21.37333112232287	29.559372730876305	27.709693203044168	21.35760294375666
58-59	21.22923838310955	28.994722696688275	28.02987655967093	21.746162360531248
60-61	21.78334680717975	29.398384534350846	27.458896745891597	21.359371912577803
62-63	20.993787549225775	29.579556975544303	28.06158900351086	21.365066471719057
64-65	21.43642004865749	28.935189892854602	27.923098685751963	21.705291372735942
66-67	21.335569053202157	29.324882074762158	27.738105008395987	21.601443863639698
68-69	21.40188733329274	29.250513181465156	27.84929733429636	21.498302150945747
70-71	21.80459850063647	29.131932101441095	27.58841655148313	21.475052846439308
72-73	21.192655626536265	29.336386389000335	28.07513568712886	21.395822297334536
74-75	21.409420493034474	28.898452284653313	27.945844523765917	21.746282698546292
76-77	21.469733906174376	29.40769869671523	27.648994708256126	21.47357268885427
78-79	21.292439908610497	29.290501503864174	27.93633782057746	21.480720766947865
80-81	21.52266374517986	29.286561465544086	27.63829127679146	21.552483512484592
82-83	21.0270717537939	29.475929720589235	28.23361872731325	21.263379798303617
84-85	21.348420613078183	28.818862388942307	27.86922057745789	21.96349642052162
86-87	21.50971891706504	29.41709362110514	27.710042028562576	21.363145433267245
88-89	21.02436392133304	29.170096408230222	28.007281642193956	21.798258028242778
90-91	21.395777643022587	29.0684438306518	27.617131217072444	21.918647309253178
92-93	21.017746212802685	29.390875057597842	28.149770156176345	21.441608573423125
94-95	21.298475121718052	28.744522416844347	27.98068733003457	21.97631513140303
96-97	21.207326207275663	29.34227893288201	27.758411048951455	21.69198381089087
98-99	20.883430131318093	29.159146380647492	28.028726750492734	21.928696737541678
100	21.27384530812522	28.993685215818704	27.614165892271036	22.118303583785035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20402.0
1	13291.0
2	5010.5
3	3128.5
4	2045.0
5	1514.5
6	1318.0
7	1337.5
8	1572.5
9	1659.5
10	1328.5
11	1068.0
12	1027.0
13	1088.0
14	1210.5
15	1287.5
16	1505.5
17	1933.5
18	2489.5
19	3334.5
20	4763.5
21	6541.0
22	9142.0
23	13440.0
24	19687.0
25	26950.0
26	35591.0
27	46166.5
28	58334.0
29	71136.0
30	82938.0
31	93583.0
32	103354.0
33	110983.5
34	116855.5
35	123142.5
36	129783.0
37	135685.0
38	143308.0
39	150365.5
40	155403.0
41	157382.5
42	154052.0
43	152050.5
44	149236.0
45	142543.0
46	136177.5
47	129168.5
48	121258.5
49	118380.0
50	117874.5
51	113943.0
52	108153.0
53	101513.5
54	95468.5
55	91238.0
56	83092.5
57	72901.0
58	66268.5
59	61008.0
60	54295.5
61	45914.0
62	38525.0
63	31442.5
64	25263.0
65	20704.0
66	16645.5
67	12932.5
68	10012.5
69	7649.5
70	5608.5
71	4330.0
72	3430.5
73	2736.0
74	2283.0
75	1751.5
76	1186.5
77	880.5
78	689.0
79	529.0
80	404.5
81	261.5
82	193.0
83	173.5
84	146.5
85	111.0
86	92.0
87	84.0
88	65.5
89	58.0
90	50.5
91	35.0
92	31.0
93	29.0
94	31.0
95	27.5
96	20.0
97	15.0
98	16.5
99	21.5
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.3694644212234863E-4
2	7.220280902621756E-5
3	2.406760300873919E-5
4	7.220280902621756E-5
5	4.813520601747838E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.2033801504369595E-5
64-65	3.610140451310878E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.406760300873919E-5
82-83	1.2033801504369593E-4
84-85	6.738928842446973E-4
86-87	0.001215413951941329
88-89	0.0031528559941448337
90-91	0.002490996911404506
92-93	0.0018411716301685477
94-95	0.0017810026226466999
96-97	0.0017087998136204824
98-99	0.002166084270786527
100	0.0026474363309613104
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4154963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.764798213297375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.40177086428967	42.97803280687681
2	15.419930404443186	17.814583365514995
3	4.816458522744052	8.346652640070795
4	2.0116330731722876	4.648063142039699
5	1.014803782893521	2.9309967872467544
6	0.571828209208286	1.9818924670552702
7	0.3581084451924269	1.4480243452512742
8	0.24280931174114295	1.1220664717629394
9	0.17897276964944953	0.9304493332027864
>10	0.8737452123408677	9.133616045510072
>50	0.06719434961171557	2.692932779387189
>100	0.03987017883937211	4.210338018349261
>500	0.0023748975595099604	0.884774095047398
>1k	4.5831345508206075E-4	0.30633269340904595
>5k	0.0	0.0
>10k+	4.166485955289826E-5	0.5712450092757052
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23735	0.5712445574124246	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.4067603008739186E-5	0.0
10-11	0.0	0.0	0.0	4.813520601747837E-5	0.0
12-13	0.0	0.0	0.0	4.813520601747837E-5	0.0
14-15	0.0	0.0	0.0	7.220280902621756E-5	0.0
16-17	0.0	0.0	0.0	9.627041203495674E-5	0.0
18-19	0.0	0.0	0.0	1.2033801504369594E-4	0.0
20-21	0.0	0.0	0.0	1.8050702256554391E-4	0.0
22-23	0.0	0.0	0.0	5.054196631835229E-4	0.0
24-25	0.0	0.0	0.0	8.423661053058715E-4	0.0
26-27	0.0	0.0	0.0	0.0013237181654806554	0.0
28-29	0.0	0.0	0.0	0.002394726499369549	0.0
30-31	0.0	0.0	0.0	0.00533097406643573	0.0
32-33	0.0	0.0	0.0	0.011456179032159853	0.0
34-35	0.0	0.0	0.0	0.02158863989883905	0.0
36-37	0.0	0.0	0.0	0.03697987202292776	0.0
38-39	0.0	0.0	0.0	0.05578870377425743	0.0
40-41	0.0	0.0	0.0	0.07577684807301532	0.0
42-43	0.0	0.0	0.0	0.09481432205292803	0.0
44-45	0.0	0.0	0.0	0.12068699528732266	0.0
46-47	0.0	0.0	0.0	0.152444197457354	0.0
48-49	0.0	0.0	0.0	0.18422546723039412	0.0
50-51	0.0	0.0	0.0	0.21729435376440176	0.0
52-53	0.0	0.0	0.0	0.25057984872548805	0.0
54-55	0.0	0.0	0.0	0.28882326990637464	0.0
56-57	0.0	0.0	0.0	0.33676593509978303	0.0
58-59	0.0	0.0	0.0	0.3916641375627172	0.0
60-61	0.0	0.0	0.0	0.44989570304236165	0.0
62-63	0.0	0.0	0.0	0.5096796289160698	0.0
64-65	0.0	0.0	0.0	0.5743974134065695	0.0
66-67	0.0	0.0	0.0	0.6521117035217883	0.0
68-69	0.0	0.0	0.0	0.7422809781940296	0.0
70-71	0.0	0.0	0.0	0.8441952431345358	0.0
72-73	0.0	0.0	0.0	0.9517533609805912	0.0
74-75	0.0	0.0	0.0	1.0619468813561035	0.0
76-77	0.0	0.0	0.0	1.1844389468690817	0.0
78-79	0.0	0.0	0.0	1.3258722159499374	0.0
80-81	0.0	0.0	0.0	1.4881841306408745	0.0
82-83	0.0	0.0	0.0	1.6608571484270738	0.0
84-85	0.0	0.0	0.0	1.8380548755789161	0.0
86-87	0.0	0.0	0.0	2.031678741784223	0.0
88	0.0	0.0	0.0	2.1885634119966895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4750	0.0	47.09872	1
GTATCAA	8040	0.0	36.945095	1
TCAACGC	9470	0.0	30.522554	4
ATCAACG	9660	0.0	29.922215	3
CAACGCA	9860	0.0	29.219938	5
AACGCAG	10485	0.0	27.522995	6
TATCAAC	11055	0.0	26.31647	2
ACGCAGA	11120	0.0	25.697718	7
CGCAGAG	11350	0.0	25.052742	8
GTACATG	17820	0.0	23.790009	1
TACATGG	17155	0.0	23.506733	2
ACATGGG	17875	0.0	21.74479	3
GCAGAGT	13000	0.0	21.222206	9
CATGGGG	11020	0.0	19.704079	4
CAGAGTA	12810	0.0	18.344954	10-11
AGAGTAC	11805	0.0	17.856329	10-11
GTGGTAT	2475	0.0	17.850418	1
CATGGGT	3155	0.0	16.08867	4
GAGTACA	13360	0.0	15.936299	1
CCCCTAT	1535	0.0	15.61556	1
>>END_MODULE