FastQCFastQC Report
Wed 25 May 2016
SRR2431348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4154963
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237470.5715333686485295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178990.4307860262534227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133450.32118216215162443No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47360.11398416784938879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85650.063.3864251
GTATCAA174100.045.276831
ATCAACG230600.034.036223
TCAACGC232300.033.787144
CAACGCA237500.033.046985
TATCAAC240300.032.7405432
AACGCAG248350.031.678916
GTGGTAT30250.030.7667391
TGGTATC29350.029.624282
ACGCAGA275000.028.4551267
CGCAGAG277600.028.1886148
GCAGAGT298350.025.9445729
GAGTACT203450.022.54608212-13
GTACATG169800.022.5334851
TACATGG166950.022.4083982
AGAGTAC287100.022.3285310-11
CAGAGTA295950.021.74022710-11
AGTACTT213400.021.46181312-13
ACATGGG168450.021.427643
GTACTTT228400.020.0111714-15
Produced by FastQC (version 0.11.5)