##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431348_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4154963
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78996948949967	34.0	31.0	34.0	31.0	34.0
2	32.907913740748114	34.0	31.0	34.0	31.0	34.0
3	32.983276626049374	34.0	31.0	34.0	31.0	34.0
4	36.37152268263279	37.0	37.0	37.0	35.0	37.0
5	36.32861399728469	37.0	37.0	37.0	35.0	37.0
6	36.36163426726063	37.0	37.0	37.0	35.0	37.0
7	36.232037445339465	37.0	37.0	37.0	35.0	37.0
8	36.28304993329664	37.0	37.0	37.0	35.0	37.0
9	38.10837737905247	39.0	39.0	39.0	37.0	39.0
10-11	38.10083399057946	39.0	39.0	39.0	36.0	39.0
12-13	38.081016966938094	39.0	39.0	39.0	35.0	39.0
14-15	39.571327951656855	41.0	40.0	41.0	37.0	41.0
16-17	39.58215680861659	41.0	40.0	41.0	37.0	41.0
18-19	39.65876771947187	41.0	40.0	41.0	37.0	41.0
20-21	39.664304230868	41.0	40.0	41.0	37.0	41.0
22-23	39.593910342883916	41.0	40.0	41.0	37.0	41.0
24-25	39.56827485587718	41.0	40.0	41.0	37.0	41.0
26-27	39.48168984898301	41.0	40.0	41.0	36.0	41.0
28-29	39.39838116488643	41.0	39.5	41.0	36.0	41.0
30-31	39.23798334666277	41.0	39.0	41.0	35.5	41.0
32-33	39.04264418239103	41.0	39.0	41.0	35.0	41.0
34-35	38.94465426045912	41.0	39.0	41.0	35.0	41.0
36-37	38.83527482675538	41.0	39.0	41.0	35.0	41.0
38-39	38.71440046517863	41.0	38.0	41.0	35.0	41.0
40-41	38.61481064933671	41.0	38.0	41.0	35.0	41.0
42-43	38.480070700990595	41.0	38.0	41.0	35.0	41.0
44-45	38.27810440189239	41.0	38.0	41.0	35.0	41.0
46-47	37.980044948655376	40.5	38.0	41.0	34.0	41.0
48-49	37.71449529153449	40.0	38.0	41.0	33.0	41.0
50-51	37.564091304784185	40.0	38.0	41.0	33.0	41.0
52-53	37.448425653850585	40.0	37.5	41.0	33.0	41.0
54-55	37.306338467995985	40.0	37.0	41.0	33.0	41.0
56-57	37.143081298196876	40.0	37.0	41.0	33.0	41.0
58-59	36.963628075629074	40.0	36.0	41.0	32.5	41.0
60-61	36.77458162684	40.0	36.0	41.0	32.0	41.0
62-63	36.55272020472866	39.0	35.0	41.0	32.0	41.0
64-65	36.291134241147276	39.0	35.0	41.0	31.0	41.0
66-67	36.012058230121426	39.0	35.0	41.0	31.0	41.0
68-69	35.749611609056444	38.0	35.0	40.5	31.0	41.0
70-71	35.497257496637154	37.0	35.0	40.0	31.0	41.0
72-73	35.23961260304846	37.0	35.0	39.5	31.0	41.0
74-75	34.9405921304233	36.0	35.0	39.0	31.0	41.0
76-77	34.62053236093799	36.0	35.0	39.0	31.0	41.0
78-79	34.305438941333534	35.5	35.0	38.0	30.5	40.0
80-81	33.99532498845357	35.0	35.0	37.0	30.0	39.0
82-83	33.674884349150645	35.0	35.0	37.0	30.0	39.0
84-85	33.408968142435924	35.0	34.5	36.0	30.0	38.5
86-87	33.18073446141398	35.0	34.0	36.0	30.0	37.0
88-89	32.95607469910081	35.0	34.0	36.0	29.0	37.0
90-91	32.75577363745477	35.0	34.0	35.0	29.0	36.5
92-93	32.60813249119186	35.0	34.0	35.0	29.0	36.0
94-95	32.47364380862116	35.0	34.0	35.0	29.0	36.0
96-97	32.33642826662957	35.0	34.0	35.0	29.0	36.0
98-99	32.21742997470736	35.0	34.0	35.0	29.0	36.0
100	32.11024117422947	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25098568646754416
1101	2	0.24624955793310477
1101	3	0.24929318950763957
1101	4	0.15126480797948716
1101	5	0.19248811291309664
1101	6	0.19209897281478305
1101	7	0.2299221664086204
1101	8	0.2558594837181545
1101	9	0.30678477777330926
1101	10-11	0.32973330566651526
1101	12-13	0.3085062050031766
1101	14-15	0.35551577869911455
1101	16-17	0.40314967635291055
1101	18-19	0.3668208719289012
1101	20-21	0.35237362608920364
1101	22-23	0.38295720303307945
1101	24-25	0.37156569347754953
1101	26-27	0.3095226760185099
1101	28-29	0.3887155964879909
1101	30-31	0.42462281990372475
1101	32-33	0.3969445791534625
1101	34-35	0.3928755237092503
1101	36-37	0.4058656964092009
1101	38-39	0.3811299419153542
1101	40-41	0.4260582027415296
1101	42-43	0.4265054697621409
1101	44-45	0.5085585122965242
1101	46-47	0.5666536269231841
1101	48-49	0.6196239024122718
1101	50-51	0.5623047859938737
1101	52-53	0.548701509378958
1101	54-55	0.5027139042243292
1101	56-57	0.44362471366501666
1101	58-59	0.499057680879595
1101	60-61	0.5064450154417415
1101	62-63	0.5022065673909566
1101	64-65	0.49590613382954274
1101	66-67	0.5090949089961754
1101	68-69	0.5144123992886733
1101	70-71	0.43210463727017867
1101	72-73	0.4477095526412924
1101	74-75	0.4004864918374551
1101	76-77	0.45019966645426734
1101	78-79	0.44737118612096083
1101	80-81	0.4393396853113032
1101	82-83	0.4714032919916633
1101	84-85	0.4394018604380392
1101	86-87	0.38634972313366944
1101	88-89	0.4195555206248187
1101	90-91	0.4400086994443839
1101	92-93	0.4532330110528946
1101	94-95	0.4656129473773234
1101	96-97	0.49343613854970414
1101	98-99	0.5364557700789376
1101	100	0.5599353337030166
1102	1	0.16265748513203704
1102	2	0.14853157839946363
1102	3	0.22129594869723235
1102	4	0.138496681834269
1102	5	0.1510443266602124
1102	6	0.16286720800013654
1102	7	0.2086873105430911
1102	8	0.22440851503874626
1102	9	0.25100505781683324
1102	10-11	0.278710580279359
1102	12-13	0.26318201207798353
1102	14-15	0.36842660519965875
1102	16-17	0.35729782397429943
1102	18-19	0.35052624547434164
1102	20-21	0.3739270687945364
1102	22-23	0.353566855929877
1102	24-25	0.3508250738925156
1102	26-27	0.3099389248955191
1102	28-29	0.2737005323557966
1102	30-31	0.25624777871737336
1102	32-33	0.3308954115326941
1102	34-35	0.30750562874334975
1102	36-37	0.22357357787782917
1102	38-39	0.3329448676139748
1102	40-41	0.3323905902701796
1102	42-43	0.3172517546935083
1102	44-45	0.2780955420905613
1102	46-47	0.23335276858765752
1102	48-49	0.3074135402804856
1102	50-51	0.24639582976874408
1102	52-53	0.27323933326965033
1102	54-55	0.31659068412680824
1102	56-57	0.330572372028044
1102	58-59	0.34224847978824613
1102	60-61	0.28852189822121943
1102	62-63	0.304623465767925
1102	64-65	0.37191208836929235
1102	66-67	0.38815256515468377
1102	68-69	0.4168108082508297
1102	70-71	0.4016923944330486
1102	72-73	0.37793524202386664
1102	74-75	0.3493841197403569
1102	76-77	0.38023548376936844
1102	78-79	0.3311889184179364
1102	80-81	0.3118682076040855
1102	82-83	0.3369717348273724
1102	84-85	0.3312873777742382
1102	86-87	0.297866258122518
1102	88-89	0.2969785228791082
1102	90-91	0.3142958495381265
1102	92-93	0.3307745351969942
1102	94-95	0.29767691127081974
1102	96-97	0.32273143109728153
1102	98-99	0.31563929648443434
1102	100	0.3715229818257484
1103	1	0.12812984461805144
1103	2	0.15560765769679108
1103	3	0.14844635240986293
1103	4	0.10762152979685169
1103	5	0.13968741238014104
1103	6	0.10467244751236393
1103	7	0.0970328293104501
1103	8	0.12955422410534112
1103	9	0.145750734568459
1103	10-11	0.20499162171881125
1103	12-13	0.20290966874945582
1103	14-15	0.23368038986530593
1103	16-17	0.2397431690487295
1103	18-19	0.231398031369757
1103	20-21	0.24365198767051055
1103	22-23	0.2445218079735696
1103	24-25	0.25085559357995635
1103	26-27	0.2602250469652887
1103	28-29	0.29407646309405067
1103	30-31	0.2720031185406242
1103	32-33	0.21647037102678723
1103	34-35	0.2420310251626745
1103	36-37	0.2373648860503721
1103	38-39	0.23345382828397732
1103	40-41	0.18254206666006212
1103	42-43	0.26017455334884687
1103	44-45	0.2520626975210476
1103	46-47	0.24879573043355663
1103	48-49	0.23141906972974624
1103	50-51	0.2454721604046739
1103	52-53	0.24310563010951114
1103	54-55	0.2515640018412384
1103	56-57	0.2528589249639879
1103	58-59	0.2538298035347992
1103	60-61	0.20641900199264285
1103	62-63	0.23099112926144016
1103	64-65	0.2293766204531167
1103	66-67	0.2471116642853275
1103	68-69	0.19098756212301282
1103	70-71	0.21873225404248586
1103	72-73	0.2628396660320931
1103	74-75	0.24099614270193115
1103	76-77	0.19270681546002066
1103	78-79	0.1371276905391028
1103	80-81	0.11845327898873137
1103	82-83	0.13304681325848122
1103	84-85	0.1419261817615407
1103	86-87	0.12203695293910499
1103	88-89	0.11848951669887242
1103	90-91	0.18037007521326842
1103	92-93	0.19325192594325102
1103	94-95	0.1812846973816704
1103	96-97	0.20612782349070358
1103	98-99	0.21301909155371135
1103	100	0.28171700625047436
1104	1	0.08255132430075207
1104	2	0.10751767432033432
1104	3	0.12153892750423978
1104	4	0.04943628143629297
1104	5	0.11717570151441947
1104	6	0.11889559340004041
1104	7	0.10371665365981642
1104	8	0.1541757077517545
1104	9	0.18296720961637902
1104	10-11	0.21339787995767523
1104	12-13	0.25565366142365775
1104	14-15	0.31017875120726757
1104	16-17	0.29826511046280046
1104	18-19	0.27694012352806396
1104	20-21	0.28135534021697595
1104	22-23	0.2570931329569319
1104	24-25	0.2781992352899323
1104	26-27	0.28084828760183456
1104	28-29	0.250579978455967
1104	30-31	0.2968018106211545
1104	32-33	0.2728389358075347
1104	34-35	0.3300142104474304
1104	36-37	0.3689191356461947
1104	38-39	0.3462744217105751
1104	40-41	0.3543206845965088
1104	42-43	0.3624540676824495
1104	44-45	0.37598521809530183
1104	46-47	0.4455093934860983
1104	48-49	0.42251339212780437
1104	50-51	0.3703009280011358
1104	52-53	0.3832835629469855
1104	54-55	0.3520322552206565
1104	56-57	0.3558635706239244
1104	58-59	0.3014291358595429
1104	60-61	0.33101597834254193
1104	62-63	0.35702917208520546
1104	64-65	0.43351391741180834
1104	66-67	0.4054877950104725
1104	68-69	0.3931445013470807
1104	70-71	0.36700973249958935
1104	72-73	0.411824804561455
1104	74-75	0.43922004413220606
1104	76-77	0.3809820856456909
1104	78-79	0.3730454512068704
1104	80-81	0.3376083519084929
1104	82-83	0.34421409121600277
1104	84-85	0.3324005866268962
1104	86-87	0.3555550012051114
1104	88-89	0.3358425545562582
1104	90-91	0.34825227664951086
1104	92-93	0.358928723253193
1104	94-95	0.3574118153738084
1104	96-97	0.37034669218956395
1104	98-99	0.41953085970612136
1104	100	0.42152174359290484
1105	1	-0.032413366099937946
1105	2	0.017981904291659134
1105	3	0.043621089734614316
1105	4	-0.058030786222033726
1105	5	-0.07010791546242956
1105	6	-0.08886506745505329
1105	7	-0.4286016419854093
1105	8	-0.34927927006801696
1105	9	-0.32812344464398535
1105	10-11	-0.3362382080902435
1105	12-13	-0.24551136757663272
1105	14-15	-0.21424649324091405
1105	16-17	-0.16953785280950484
1105	18-19	-0.213416697962046
1105	20-21	-0.1981318030629282
1105	22-23	-0.21060808519045082
1105	24-25	-0.1888571714381868
1105	26-27	-0.24915147204352195
1105	28-29	-0.22098222157017489
1105	30-31	-0.16265991515523126
1105	32-33	-0.19094758119830146
1105	34-35	-0.2242695252045266
1105	36-37	-0.26571219788642964
1105	38-39	-0.23324841423387
1105	40-41	-0.20425291575079996
1105	42-43	-0.2696099778166854
1105	44-45	-0.2632253971675169
1105	46-47	-0.2185304422915877
1105	48-49	-0.21879859871467744
1105	50-51	-0.21204059191149582
1105	52-53	-0.2657177334658769
1105	54-55	-0.25501905899346156
1105	56-57	-0.2306440313427771
1105	58-59	-0.24168444823379076
1105	60-61	-0.23420996253322102
1105	62-63	-0.25165267798456625
1105	64-65	-0.20258279829221237
1105	66-67	-0.2000307325769768
1105	68-69	-0.22590923744449753
1105	70-71	-0.24215485484904775
1105	72-73	-0.1872212879480486
1105	74-75	-0.22623656209027843
1105	76-77	-0.2505174719515466
1105	78-79	-0.1897101997561279
1105	80-81	-0.1783426638152079
1105	82-83	-0.18527539552167838
1105	84-85	-0.22597810112689132
1105	86-87	-0.23635844708784504
1105	88-89	-0.20160085319187715
1105	90-91	-0.1968344838656222
1105	92-93	-0.18976152420693637
1105	94-95	-0.19769768869944926
1105	96-97	-0.1476064326461355
1105	98-99	-0.1616178101562511
1105	100	-0.19312057114249015
1106	1	0.07877168112342048
1106	2	0.06281400515236868
1106	3	0.040742118178833664
1106	4	0.03358913464991531
1106	5	0.057498846789741265
1106	6	0.07379426315251436
1106	7	0.09623106500937695
1106	8	0.058666706854808126
1106	9	0.07698494314957571
1106	10-11	0.11329162472696197
1106	12-13	0.10510077273789165
1106	14-15	0.08921464254413536
1106	16-17	0.12429627584521086
1106	18-19	0.11917499359285699
1106	20-21	0.09030282813203172
1106	22-23	0.14646825464137692
1106	24-25	0.11304187417614742
1106	26-27	0.09681349340683454
1106	28-29	0.15191857081237714
1106	30-31	0.14037273894057734
1106	32-33	0.13693610167234738
1106	34-35	0.10932000079862547
1106	36-37	0.1558059200121562
1106	38-39	0.14526991856681093
1106	40-41	0.1130579979687667
1106	42-43	0.17415394949130558
1106	44-45	0.17246923248456625
1106	46-47	0.21122539588163391
1106	48-49	0.24791204940795808
1106	50-51	0.23120239070898663
1106	52-53	0.22339846794697138
1106	54-55	0.19109542871303375
1106	56-57	0.18026384729816414
1106	58-59	0.19108043235779348
1106	60-61	0.19054634401633308
1106	62-63	0.1634000281285637
1106	64-65	0.2375287854810253
1106	66-67	0.21617875287029165
1106	68-69	0.13364426215323988
1106	70-71	0.16359217784913227
1106	72-73	0.14620576657095796
1106	74-75	0.18047127577437294
1106	76-77	0.15976473856432705
1106	78-79	0.11691536918335999
1106	80-81	0.13959873955048607
1106	82-83	0.15060636425085505
1106	84-85	0.18916147502007874
1106	86-87	0.15962561158593047
1106	88-89	0.2203472066280483
1106	90-91	0.21359937037566112
1106	92-93	0.15929563423652127
1106	94-95	0.18579430482042625
1106	96-97	0.16514946614049109
1106	98-99	0.16876942686420904
1106	100	0.13318366408161353
1107	1	-0.1277777077324984
1107	2	-0.09633852083467787
1107	3	-0.06487377947712503
1107	4	-0.12296057900825019
1107	5	-0.1551454561598078
1107	6	-0.1016621413172274
1107	7	-0.11533637520886231
1107	8	-0.05586286295035592
1107	9	-0.06167915286655301
1107	10-11	-0.08159796066106395
1107	12-13	-0.07356286282328739
1107	14-15	-0.042316127500839684
1107	16-17	-0.027945557002389876
1107	18-19	-0.072591427166671
1107	20-21	-0.06323291176938284
1107	22-23	-0.072213930949097
1107	24-25	-0.10248440385380775
1107	26-27	-0.13297850514722143
1107	28-29	-0.09833463164221001
1107	30-31	-0.06472583471902027
1107	32-33	-0.1053936217069591
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2216	30-31	-0.6009544270441154
2216	32-33	-0.6109846419675762
2216	34-35	-0.5307974160311204
2216	36-37	-0.6028083538418443
2216	38-39	-0.6309427956596068
2216	40-41	-0.5914978343267947
2216	42-43	-0.5748483039630017
2216	44-45	-0.6072600564912989
2216	46-47	-0.5720175027722192
2216	48-49	-0.6601492386190841
2216	50-51	-0.5734157080871967
2216	52-53	-0.5367821049048302
2216	54-55	-0.6179547106453285
2216	56-57	-0.6307691835978773
2216	58-59	-0.7554867561614529
2216	60-61	-0.7586567930292745
2216	62-63	-0.8583243091779593
2216	64-65	-0.74136435334362
2216	66-67	-0.6665474820819668
2216	68-69	-0.6716380126495807
2216	70-71	-0.6440013701553795
2216	72-73	-0.7149510560584673
2216	74-75	-0.6566491950789768
2216	76-77	-0.6384614074357913
2216	78-79	-0.6350645572828668
2216	80-81	-0.670786423231938
2216	82-83	-0.6552778200579894
2216	84-85	-0.610776258479298
2216	86-87	-0.6281516225954107
2216	88-89	-0.5176546317162618
2216	90-91	-0.5183426031759026
2216	92-93	-0.7125324805710527
2216	94-95	-0.642397594604347
2216	96-97	-0.7573873007455845
2216	98-99	-0.7334714258903432
2216	100	-0.7140736448195284
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77.0
3	515.0
4	250.0
5	351.0
6	414.0
7	803.0
8	1454.0
9	2301.0
10	2883.0
11	3953.0
12	6219.0
13	9244.0
14	10174.0
15	13579.0
16	17723.0
17	27508.0
18	33442.0
19	17715.0
20	10495.0
21	9181.0
22	8864.0
23	9118.0
24	10224.0
25	12324.0
26	15510.0
27	19606.0
28	24204.0
29	30997.0
30	38838.0
31	49783.0
32	62381.0
33	81091.0
34	114701.0
35	192188.0
36	340413.0
37	764227.0
38	1381920.0
39	809735.0
40	20558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.37643710876282	21.985486480195853	14.955246089343186	23.68283032169814
2	13.514010477111462	22.42463348641937	40.45416059160108	23.607195444868086
3	15.048183607946203	28.990334397127278	32.04166606497326	23.919815929953263
4	10.414264358910856	18.849400184218045	40.29633737270025	30.439998084170846
5	11.942681559378507	38.33786245509286	35.7456612730366	13.973794712492024
6	27.731197606332476	40.34144227036438	18.486398073821597	13.440962049481547
7	23.968396349137162	34.20762110276313	24.741255216953796	17.082727331145907
8	24.150058616647126	35.36120538257501	21.841734812078954	18.647001188698916
9	25.123954172395756	15.73578392876182	22.73952860711395	36.40073329172847
10-11	22.890937897641926	27.366404947529016	29.700914304170702	20.041742850658355
12-13	23.908287510622838	26.02594776415578	30.00668116659523	20.059083558626153
14-15	21.654224117037867	26.56627748550348	28.359482382875612	23.42001601458304
16-17	20.032609195316542	30.062000552110813	28.66380518911961	21.24158506345303
18-19	19.661859804768415	27.972475326495083	32.33409058034933	20.031574288387166
20-21	21.44755561000182	27.073056968257	31.704590389854253	19.774797031886926
22-23	20.64418189344783	27.382237133450143	32.0661330072973	19.907447965804725
24-25	20.5522534764491	27.07550834448868	31.800092804788882	20.572145374273337
26-27	20.429500528587337	27.507913767759725	31.83750436979573	20.225081333857204
28-29	20.177644602489817	27.21051523192356	32.26520334302063	20.34663682256599
30-31	20.823704117317185	27.451569114946484	31.50379432427814	20.22093244345819
32-33	19.934088124790904	27.528094079363818	32.321344761107056	20.216473034738218
34-35	20.63682226931972	26.88223338243791	31.806887485217178	20.674056863025196
36-37	20.92338311139972	27.506911570051905	31.2619582827945	20.307747035753874
38-39	20.54466335809149	27.345795044839054	31.567790491135682	20.541751105933773
40-41	20.804493377099305	27.427562550152523	31.220549308202077	20.547394764546095
42-43	20.388684126035937	27.67714928391299	31.526384724503977	20.4077818655471
44-45	20.77904808550299	27.199883030872268	30.979381784465126	21.041687099159613
46-47	21.03657667040008	27.79595412124302	30.433023520510694	20.7344456878462
48-49	20.893878386530666	27.692915321705748	30.241821596657697	21.17138469510589
50-51	21.404109918737383	27.897886940892302	29.41085083626078	21.287152304109533
52-53	20.837134009889862	28.206322945913826	29.93834841329475	21.018194630901558
54-55	21.262687155207153	27.921097787080225	29.58534544598172	21.230869611730903
56-57	21.159669164499565	28.06881455425897	29.63172709079685	21.139789190444617
58-59	20.932258449988886	27.49406337986559	30.050471349527662	21.52320682061786
60-61	21.496110540038178	27.900945547560536	29.403792635622068	21.199151276779222
62-63	20.735046256729603	27.96171710795018	30.166189205535645	21.137047429784573
64-65	21.081872449887037	27.400183828351782	29.970772302906184	21.547171418854994
66-67	21.173700242218395	27.8180151376871	29.721561962084543	21.286722658009957
68-69	21.216110627014697	27.730364943177804	29.721407160570163	21.332117269237337
70-71	21.62864041129561	27.653653705933795	29.390446705860025	21.32725917691057
72-73	21.051533968389815	27.879508303151525	29.821862240019964	21.247095488438696
74-75	21.332423720110295	27.482818695463973	29.588066272439427	21.596691311986305
76-77	21.382698411628738	27.995765530639616	29.362244726047116	21.259291331684533
78-79	21.18318722919108	27.887864318699336	29.572628837234376	21.35631961487521
80-81	21.410979585932758	27.865289635514827	29.307023056230324	21.41670772232209
82-83	20.928692773655687	28.022735961778285	29.8762874960995	21.17228376846653
84-85	21.234298281831933	27.45072437624844	29.427585781144494	21.887391560775125
86-87	21.269728266415594	28.017688290983866	29.33471914958344	21.377864293017105
88-89	20.789889141722924	27.760888079242353	29.598826638285715	21.850396140749012
90-91	21.177428636549724	27.697175354455577	29.215048377900466	21.91034763109423
92-93	20.78497796540412	28.015682952130412	29.7106740829348	21.488664999530666
94-95	20.970402990387523	27.391127082564665	29.528331880836014	22.110138046211798
96-97	20.93267519730783	27.93769136449784	29.33963807011322	21.789995368081108
98-99	20.522980065282553	27.690281891432257	29.629985303964368	22.156752739320826
100	20.98844233925898	27.618958222016836	29.12013035462427	22.272469084099914
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2164.0
1	1440.5
2	2359.0
3	3346.5
4	1722.0
5	991.0
6	2328.5
7	6436.5
8	11197.5
9	11660.5
10	7287.0
11	3553.0
12	2980.0
13	3225.5
14	3604.0
15	3997.0
16	4517.5
17	5156.0
18	5924.0
19	6862.0
20	8120.0
21	9935.5
22	12635.0
23	16700.5
24	22489.5
25	29575.5
26	37994.5
27	48498.0
28	60562.5
29	73107.0
30	84537.5
31	95110.5
32	104641.0
33	111566.5
34	117359.5
35	123742.0
36	129870.5
37	135215.0
38	142218.0
39	148598.5
40	154177.5
41	156980.5
42	153559.5
43	151789.0
44	149525.0
45	142803.5
46	135593.5
47	127722.5
48	119150.5
49	115660.5
50	116065.0
51	112557.0
52	105934.0
53	99076.0
54	92121.5
55	86700.0
56	79136.0
57	69427.5
58	63039.0
59	58565.5
60	51938.5
61	43510.0
62	36437.5
63	29956.5
64	23775.5
65	18679.5
66	14750.0
67	11378.0
68	8653.5
69	6399.0
70	4442.5
71	3235.0
72	2574.5
73	2088.0
74	1711.5
75	1246.0
76	767.0
77	541.0
78	432.5
79	290.0
80	168.5
81	82.0
82	36.0
83	25.0
84	18.5
85	14.0
86	9.0
87	5.0
88	5.5
89	6.5
90	5.0
91	2.0
92	1.0
93	1.5
94	7.5
95	13.0
96	10.5
97	8.5
98	6.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008519931465093672
2	2.406760300873919E-5
3	5.535548692010014E-4
4	0.0025030307129088754
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.220280902621756E-5
24-25	1.2033801504369593E-4
26-27	4.933858616791534E-4
28-29	9.145689143320891E-4
30-31	0.0017569350196379606
32-33	0.004163695320511879
34-35	0.005403176875461948
36-37	0.003718444664850205
38-39	0.002478963109900136
40-41	0.0032972616121972683
42-43	5.05419663183523E-4
44-45	4.933858616791534E-4
46-47	0.003429633428745334
48-49	0.002466929308395767
50-51	9.386365173408282E-4
52-53	2.406760300873919E-5
54-55	5.415210676966317E-4
56-57	6.738928842446973E-4
58-59	7.581294947752844E-4
60-61	3.8508164813982703E-4
62-63	0.0
64-65	0.0
66-67	8.423661053058715E-4
68-69	6.859266857490668E-4
70-71	0.0022262532783083747
72-73	1.0830421353932636E-4
74-75	0.002009644851229722
76-77	3.6101404513108783E-4
78-79	0.0012033801504369593
80-81	8.182985022971323E-4
82-83	0.0011432111429151113
84-85	0.0010469407308801547
86-87	2.6474363309613103E-4
88-89	0.0015282927910549382
90-91	0.001684732210611743
92-93	0.003200991200162312
94-95	0.0012033801504369593
96-97	0.002346591293352071
98-99	0.002430827903882658
100	0.0034416672302497037
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4154963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.369684054506315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36252644787675	39.34327700356916
2	16.420301650093887	17.855332255905868
3	5.280282036767649	8.612617981732265
4	2.215864595010216	4.819034317530883
5	1.131307859767243	3.0754425451962373
6	0.6276774965499435	2.047597630532634
7	0.38472410033977544	1.4642129448539443
8	0.27152782874910353	1.1810305808875887
9	0.1960438783886125	0.9592959355928028
>10	0.9819233859110656	9.69429330439662
>50	0.07657951408801543	2.911666252272644
>100	0.04725699569059988	4.794693561605831
>500	0.0030545616742243104	1.1027657154622768
>1k	7.968420796952472E-4	0.8151661094496246
>5k	0.0	0.0
>10k+	1.328070132825342E-4	1.3235738610116632
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23747	0.5715333686485295	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17899	0.4307860262534227	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13345	0.32118216215162443	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4736	0.11398416784938879	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.4067603008739186E-5	0.0
5	0.0	0.0	0.0	2.4067603008739186E-5	0.0
6	0.0	0.0	0.0	2.4067603008739186E-5	0.0
7	0.0	0.0	0.0	2.4067603008739186E-5	0.0
8	0.0	0.0	0.0	2.4067603008739186E-5	0.0
9	0.0	0.0	0.0	4.813520601747837E-5	0.0
10-11	0.0	2.4067603008739186E-5	0.0	6.0169007521847965E-5	0.0
12-13	0.0	2.4067603008739186E-5	0.0	7.220280902621756E-5	0.0
14-15	0.0	2.4067603008739186E-5	0.0	1.4440561805243511E-4	0.0
16-17	0.0	2.4067603008739186E-5	0.0	1.6847322106117431E-4	0.0
18-19	0.0	2.4067603008739186E-5	0.0	1.925408240699135E-4	0.0
20-21	0.0	2.4067603008739186E-5	0.0	2.5270983159176143E-4	0.0
22-23	0.0	2.4067603008739186E-5	0.0	5.776224722097406E-4	0.0
24-25	0.0	2.4067603008739186E-5	0.0	9.266027158364588E-4	0.0
26-27	0.0	2.4067603008739186E-5	0.0	0.0013838871730025033	0.0
28-29	0.0	2.4067603008739186E-5	0.0	0.002490996911404506	0.0
30-31	0.0	2.4067603008739186E-5	0.0	0.005475379684488165	0.0
32-33	0.0	2.4067603008739186E-5	0.0	0.011600584650212287	0.0
34-35	0.0	2.4067603008739186E-5	0.0	0.021769146921404595	0.0
36-37	0.0	2.4067603008739186E-5	0.0	0.03734088606805885	0.0
38-39	0.0	2.4067603008739186E-5	0.0	0.05613768401788416	0.0
40-41	0.0	2.4067603008739186E-5	0.0	0.07621006492717264	0.0
42-43	0.0	2.4067603008739186E-5	0.0	0.09534380931912029	0.0
44-45	0.0	2.4067603008739186E-5	0.0	0.12151732759112416	0.0
46-47	0.0	2.4067603008739186E-5	0.0	0.15358740860026912	0.0
48-49	0.0	2.4067603008739186E-5	0.0	0.18564545580790973	0.0
50-51	0.0	2.4067603008739186E-5	0.0	0.21924382960810962	0.0
52-53	0.0	2.4067603008739186E-5	0.0	0.2530588118353882	0.0
54-55	0.0	2.4067603008739186E-5	0.0	0.29161511185538835	0.0
56-57	0.0	2.4067603008739186E-5	0.0	0.3400631967119804	0.0
58-59	0.0	2.4067603008739186E-5	0.0	0.395599190654646	0.0
60-61	0.0	2.4067603008739186E-5	0.0	0.454564818026057	0.0
62-63	0.0	2.4067603008739186E-5	0.0	0.515094839593036	0.0
64-65	0.0	2.4067603008739186E-5	0.0	0.5804263479602586	0.0
66-67	0.0	2.4067603008739186E-5	0.0	0.6592236802108706	0.0
68-69	0.0	2.4067603008739186E-5	0.0	0.7503797266064705	0.0
70-71	0.0	2.4067603008739186E-5	0.0	0.8537019463229878	0.0
72-73	0.0	2.4067603008739186E-5	0.0	0.9622588696939058	0.0
74-75	0.0	2.4067603008739186E-5	0.0	1.0741491560815344	0.0
76-77	0.0	2.4067603008739186E-5	0.0	1.1986267988427333	0.0
78-79	0.0	2.4067603008739186E-5	0.0	1.342358524010924	0.0
80-81	0.0	2.4067603008739186E-5	0.0	1.5073299088343266	0.0
82-83	0.0	2.4067603008739186E-5	0.0	1.6821690108913123	0.0
84-85	0.0	2.4067603008739186E-5	0.0	1.8617373969395155	0.0
86-87	0.0	2.4067603008739186E-5	0.0	2.058189206498349	0.0
88	0.0	2.4067603008739186E-5	0.0	2.2179740228733684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8565	0.0	63.386425	1
GTATCAA	17410	0.0	45.27683	1
ATCAACG	23060	0.0	34.03622	3
TCAACGC	23230	0.0	33.78714	4
CAACGCA	23750	0.0	33.04698	5
TATCAAC	24030	0.0	32.740543	2
AACGCAG	24835	0.0	31.67891	6
GTGGTAT	3025	0.0	30.766739	1
TGGTATC	2935	0.0	29.62428	2
ACGCAGA	27500	0.0	28.455126	7
CGCAGAG	27760	0.0	28.188614	8
GCAGAGT	29835	0.0	25.944572	9
GAGTACT	20345	0.0	22.546082	12-13
GTACATG	16980	0.0	22.533485	1
TACATGG	16695	0.0	22.408398	2
AGAGTAC	28710	0.0	22.32853	10-11
CAGAGTA	29595	0.0	21.740227	10-11
AGTACTT	21340	0.0	21.461813	12-13
ACATGGG	16845	0.0	21.42764	3
GTACTTT	22840	0.0	20.01117	14-15
>>END_MODULE