FastQCFastQC Report
Wed 25 May 2016
SRR2431347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834968
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126670.33030262573247027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.043.4997371
GTATCAA65500.036.0211521
TCAACGC73150.031.0333044
ATCAACG73700.030.737943
TATCAAC84100.027.7750972
CAACGCA82650.027.5231255
AACGCAG84300.026.8171566
GTACATG149400.025.7019841
TACATGG147550.024.7819562
ACGCAGA91850.024.1011057
CGCAGAG93350.023.6131388
ACATGGG157250.022.5658423
CATGGGG95700.019.4481584
CATGGGT42000.019.2475014
GCAGAGT110300.019.1322489
GTACACG3801.2732926E-1118.5525191
ATAGGAC11050.017.013473
AGAGTAC98100.016.98409510-11
GAGTACA109250.016.3047071
CAGAGTA107150.016.11982710-11
Produced by FastQC (version 0.11.5)