FastQCFastQC Report
Wed 25 May 2016
SRR2431347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834968
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159520.4159617498764005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122920.32052418690325446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87950.22933698534120753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69950.058.2576451
GTATCAA142750.048.1385271
ATCAACG187850.036.1775363
TCAACGC188500.036.027854
TATCAAC193950.035.11242
CAACGCA197500.034.361835
AACGCAG203250.033.389736
ACGCAGA228100.029.6285117
CGCAGAG229850.029.321148
GTGGTAT28000.027.0264741
GCAGAGT254150.026.406719
TGGTATC27900.025.436342
GTACATG146400.025.2670881
TACATGG144800.024.6351452
ACATGGG148250.023.33273
AGAGTAC241350.022.13086310-11
GAGTACT166700.021.59580412-13
AGTACTT175850.021.3941612-13
CAGAGTA250700.020.9024310-11
CATGGGT37750.019.4218034
Produced by FastQC (version 0.11.5)