##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431347_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3834968
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77677962371524	34.0	31.0	34.0	31.0	34.0
2	32.902219001566635	34.0	31.0	34.0	31.0	34.0
3	32.97449235560766	34.0	31.0	34.0	31.0	34.0
4	36.364863279172084	37.0	37.0	37.0	35.0	37.0
5	36.31833903177289	37.0	37.0	37.0	35.0	37.0
6	36.34996850038905	37.0	37.0	37.0	35.0	37.0
7	36.21862789989382	37.0	37.0	37.0	35.0	37.0
8	36.27019130276967	37.0	37.0	37.0	35.0	37.0
9	38.08743175953489	39.0	39.0	39.0	35.0	39.0
10-11	38.080749695955745	39.0	39.0	39.0	36.0	39.0
12-13	38.067242151694614	39.0	39.0	39.0	35.0	39.0
14-15	39.5671579267415	41.0	40.0	41.0	37.0	41.0
16-17	39.576562698828255	41.0	40.0	41.0	37.0	41.0
18-19	39.64090795542492	41.0	40.0	41.0	37.0	41.0
20-21	39.648027832305246	41.0	40.0	41.0	37.0	41.0
22-23	39.58379000815652	41.0	40.0	41.0	37.0	41.0
24-25	39.56173310963742	41.0	40.0	41.0	37.0	41.0
26-27	39.48203218384091	41.0	40.0	41.0	36.0	41.0
28-29	39.393424795200374	41.0	39.0	41.0	36.0	41.0
30-31	39.24487479426165	41.0	39.0	41.0	35.5	41.0
32-33	39.062475358334154	41.0	39.0	41.0	35.0	41.0
34-35	38.968122550175124	41.0	39.0	41.0	35.0	41.0
36-37	38.859779012497626	41.0	39.0	41.0	35.0	41.0
38-39	38.732593075092154	41.0	38.0	41.0	35.0	41.0
40-41	38.631624696738015	41.0	38.0	41.0	35.0	41.0
42-43	38.497046389956836	41.0	38.0	41.0	35.0	41.0
44-45	38.29986821272042	41.0	38.0	41.0	34.5	41.0
46-47	38.01064154902987	40.5	38.0	41.0	34.0	41.0
48-49	37.759424328964414	40.0	38.0	41.0	33.0	41.0
50-51	37.60964615610874	40.0	38.0	41.0	33.0	41.0
52-53	37.48707016590491	40.0	37.5	41.0	33.0	41.0
54-55	37.338596045651485	40.0	37.0	41.0	33.0	41.0
56-57	37.17400458100302	40.0	37.0	41.0	33.0	41.0
58-59	36.99051973836548	40.0	36.0	41.0	32.5	41.0
60-61	36.798870290443105	40.0	36.0	41.0	32.0	41.0
62-63	36.57558798404576	39.0	35.0	41.0	32.0	41.0
64-65	36.30966764259832	39.0	35.0	41.0	31.0	41.0
66-67	36.03534058172063	38.5	35.0	41.0	31.0	41.0
68-69	35.775657058937654	37.5	35.0	40.5	31.0	41.0
70-71	35.52876542907268	37.0	35.0	40.0	31.0	41.0
72-73	35.27877077461924	37.0	35.0	39.5	31.0	41.0
74-75	34.97802836946749	36.0	35.0	39.0	31.0	41.0
76-77	34.6563752292066	36.0	35.0	39.0	31.0	41.0
78-79	34.335816752577855	35.5	35.0	37.5	31.0	39.5
80-81	34.02207215288367	35.0	35.0	37.0	30.0	39.0
82-83	33.700644047095054	35.0	35.0	37.0	30.0	39.0
84-85	33.43169564909016	35.0	34.5	36.0	30.0	38.0
86-87	33.20400626550209	35.0	34.0	36.0	30.0	37.0
88-89	32.97751897799408	35.0	34.0	36.0	30.0	37.0
90-91	32.779961658089455	35.0	34.0	35.0	29.0	36.5
92-93	32.634314810449524	35.0	34.0	35.0	29.0	36.0
94-95	32.50442311383041	35.0	34.0	35.0	29.0	36.0
96-97	32.378294160472784	35.0	34.0	35.0	29.0	36.0
98-99	32.26484145369662	35.0	34.0	35.0	29.0	36.0
100	32.16357841838576	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2621303808412776
1101	2	0.25692843766064044
1101	3	0.2692199429931321
1101	4	0.18616787742059415
1101	5	0.1753561906850294
1101	6	0.21532001590500727
1101	7	0.2534561721884998
1101	8	0.27671788503002404
1101	9	0.35976251704764906
1101	10-11	0.3399174044794506
1101	12-13	0.3231994937407876
1101	14-15	0.44553933689690695
1101	16-17	0.47892030939712527
1101	18-19	0.4189808163128319
1101	20-21	0.4393670757824779
1101	22-23	0.4255421544678839
1101	24-25	0.41417580076828386
1101	26-27	0.4012474519447906
1101	28-29	0.4381552912564928
1101	30-31	0.4576414302152756
1101	32-33	0.47724024510020513
1101	34-35	0.4103636967774662
1101	36-37	0.4254556933305693
1101	38-39	0.4428608831553049
1101	40-41	0.46350846295278814
1101	42-43	0.4034961629901943
1101	44-45	0.42789910315187285
1101	46-47	0.4626477146751071
1101	48-49	0.37530585101727354
1101	50-51	0.39270146324287936
1101	52-53	0.37956244746865053
1101	54-55	0.43282151168608607
1101	56-57	0.4318290423678377
1101	58-59	0.42891244611472246
1101	60-61	0.43141617400100074
1101	62-63	0.436955001550821
1101	64-65	0.48296706464979167
1101	66-67	0.4514264008272164
1101	68-69	0.4182296164280359
1101	70-71	0.3865255537199417
1101	72-73	0.3807117361272958
1101	74-75	0.3731257519235811
1101	76-77	0.40697559931095384
1101	78-79	0.37731146174261454
1101	80-81	0.39300176860849234
1101	82-83	0.38332494473932144
1101	84-85	0.37200930114931907
1101	86-87	0.40287319721801396
1101	88-89	0.44423852204152325
1101	90-91	0.43346605345851685
1101	92-93	0.47306409368470526
1101	94-95	0.4698680745176276
1101	96-97	0.5327229226562906
1101	98-99	0.476968203026054
1101	100	0.5437570242998717
1102	1	0.13744159730617156
1102	2	0.16572528721189883
1102	3	0.19666486286849505
1102	4	0.1324993385246671
1102	5	0.1693127217138084
1102	6	0.1790488951004292
1102	7	0.21944491942858235
1102	8	0.22969340447636455
1102	9	0.30603672922209313
1102	10-11	0.30048849708991554
1102	12-13	0.28574865802214333
1102	14-15	0.3888296791652195
1102	16-17	0.3650361673433906
1102	18-19	0.3597596556329137
1102	20-21	0.37465195267666473
1102	22-23	0.3739011245686825
1102	24-25	0.34642317530995115
1102	26-27	0.41141803350277684
1102	28-29	0.4039816222344328
1102	30-31	0.36231225212473817
1102	32-33	0.3922531422027191
1102	34-35	0.3959953365502713
1102	36-37	0.4095922608511131
1102	38-39	0.398110284518836
1102	40-41	0.4335979224520088
1102	42-43	0.4145407038870488
1102	44-45	0.46590969814581484
1102	46-47	0.46565948574940563
1102	48-49	0.42482228742727557
1102	50-51	0.41585903500873656
1102	52-53	0.4159587890052734
1102	54-55	0.3965276793667982
1102	56-57	0.36294266749736437
1102	58-59	0.40449022632598997
1102	60-61	0.39139042030058135
1102	62-63	0.3945697276101683
1102	64-65	0.44833554885001803
1102	66-67	0.42615463296146316
1102	68-69	0.3773167936370143
1102	70-71	0.35215200166941685
1102	72-73	0.35422454057551533
1102	74-75	0.3597855217156223
1102	76-77	0.3591362165896754
1102	78-79	0.37641149988927936
1102	80-81	0.3424199885667605
1102	82-83	0.3337170293858591
1102	84-85	0.38470261483598023
1102	86-87	0.40705101289974976
1102	88-89	0.3608579333272317
1102	90-91	0.3791882094669461
1102	92-93	0.37872131139684484
1102	94-95	0.39077757520714584
1102	96-97	0.4057167509106563
1102	98-99	0.46290870854964794
1102	100	0.47888483732710796
1103	1	0.17849758273438
1103	2	0.20618334333597232
1103	3	0.21181081569635296
1103	4	0.15050767949966826
1103	5	0.17757077948910904
1103	6	0.21279506845252882
1103	7	0.1960675989205427
1103	8	0.21025262389001398
1103	9	0.2620167909381337
1103	10-11	0.30555538033306817
1103	12-13	0.2898566907016402
1103	14-15	0.34398053300626685
1103	16-17	0.37754641989860005
1103	18-19	0.33871109930371546
1103	20-21	0.3636949213099001
1103	22-23	0.388945939729453
1103	24-25	0.3516507685124708
1103	26-27	0.3648432125541703
1103	28-29	0.4032506694962592
1103	30-31	0.4197451731542259
1103	32-33	0.3930068704887262
1103	34-35	0.38882602113484666
1103	36-37	0.3193310922206649
1103	38-39	0.39275991687878786
1103	40-41	0.4217486234121992
1103	42-43	0.41297947567008464
1103	44-45	0.43176897401435355
1103	46-47	0.39724536992036974
1103	48-49	0.36569849549215405
1103	50-51	0.3292580036749868
1103	52-53	0.33446976496881575
1103	54-55	0.3395606962244031
1103	56-57	0.3354909438296616
1103	58-59	0.3346893643284474
1103	60-61	0.2879110710321413
1103	62-63	0.3212586910636688
1103	64-65	0.358172991224329
1103	66-67	0.33871610202984925
1103	68-69	0.3262516310865067
1103	70-71	0.35263754787971635
1103	72-73	0.34672078047491084
1103	74-75	0.30211228306973226
1103	76-77	0.29750985457633305
1103	78-79	0.26967218681688365
1103	80-81	0.2799661268445135
1103	82-83	0.3432947878107413
1103	84-85	0.3552334046720489
1103	86-87	0.3232303421541758
1103	88-89	0.29691401697498776
1103	90-91	0.3705651735376705
1103	92-93	0.36368485913254744
1103	94-95	0.3728033263802999
1103	96-97	0.40219120286692345
1103	98-99	0.4445342150564855
1103	100	0.5097264877685674
1104	1	0.18221438700320647
1104	2	0.20691913385513772
1104	3	0.19500988214352333
1104	4	0.09555499950312907
1104	5	0.1383401419728969
1104	6	0.16233033176791167
1104	7	0.12303971336640984
1104	8	0.1522293579989551
1104	9	0.20853210638802722
1104	10-11	0.24649848301069
1104	12-13	0.25204757124249966
1104	14-15	0.3019401952326888
1104	16-17	0.29781670790484327
1104	18-19	0.26314652587176823
1104	20-21	0.30066165330875805
1104	22-23	0.33767364994813676
1104	24-25	0.32341646483001796
1104	26-27	0.3300669287955671
1104	28-29	0.3460470496179795
1104	30-31	0.358358808104839
1104	32-33	0.37140613169442815
1104	34-35	0.36565362607920804
1104	36-37	0.36471830942316785
1104	38-39	0.37317644561855445
1104	40-41	0.3768642347343132
1104	42-43	0.44175297119123513
1104	44-45	0.3800362231976422
1104	46-47	0.4545792888552782
1104	48-49	0.41133546946637267
1104	50-51	0.4234160529127422
1104	52-53	0.38580300981244875
1104	54-55	0.35634184136909397
1104	56-57	0.3473676419541505
1104	58-59	0.3829286323643615
1104	60-61	0.4206097343031914
1104	62-63	0.37992085192671965
1104	64-65	0.4162771358911854
1104	66-67	0.38982785200774117
1104	68-69	0.35410193817215685
1104	70-71	0.3831699960995465
1104	72-73	0.3783805252578958
1104	74-75	0.3935636670777569
1104	76-77	0.3847810473018285
1104	78-79	0.33965389217986797
1104	80-81	0.3458926292464213
1104	82-83	0.32513525319080117
1104	84-85	0.34273968030353785
1104	86-87	0.3746735225985489
1104	88-89	0.3512271254727679
1104	90-91	0.36256302881248814
1104	92-93	0.4157171760046978
1104	94-95	0.4010341797762109
1104	96-97	0.44422379440894133
1104	98-99	0.4358366684605315
1104	100	0.3828422338548094
1105	1	-0.01347191746027221
1105	2	0.07282584246360813
1105	3	0.11803988291399747
1105	4	-0.061270203653521094
1105	5	-0.008078155463451253
1105	6	0.0021806366048124914
1105	7	-0.36603579802189756
1105	8	-0.315149141152105
1105	9	-0.2893698216136542
1105	10-11	-0.293172449225942
1105	12-13	-0.17188899419238624
1105	14-15	-0.11963437303082713
1105	16-17	-0.033545906478678944
1105	18-19	-0.03678647773813992
1105	20-21	-0.10588409036531488
1105	22-23	-0.07516288168901752
1105	24-25	-0.10941650897598265
1105	26-27	-0.11568308547402495
1105	28-29	-0.04823773895648742
1105	30-31	-0.07529551215554164
1105	32-33	-0.11101992973490837
1105	34-35	-0.17610727402212234
1105	36-37	-0.17239106759194556
1105	38-39	-0.14229807303834718
1105	40-41	-0.15626855873392742
1105	42-43	-0.08743522260039072
1105	44-45	-0.04321205667087469
1105	46-47	-0.0378338941571954
1105	48-49	-0.08657978792141563
1105	50-51	-0.01827525481319725
1105	52-53	-0.07832425071248394
1105	54-55	-0.04831097970977538
1105	56-57	-0.08674759882597982
1105	58-59	-0.07562795986575566
1105	60-61	-0.08094828954625655
1105	62-63	0.0017796140178134578
1105	64-65	0.023959305416020982
1105	66-67	-0.00404219463179345
1105	68-69	0.001353313919565835
1105	70-71	-0.08707671735809441
1105	72-73	-0.06163770321843742
1105	74-75	-0.059814849712687135
1105	76-77	-0.066094823164498
1105	78-79	-0.09814310476436106
1105	80-81	-0.10193265894538683
1105	82-83	-0.08928957215013611
1105	84-85	-0.04179130918787877
1105	86-87	-0.04141042025050723
1105	88-89	-0.055640969684738195
1105	90-91	0.006917251673499436
1105	92-93	0.006157094004848318
1105	94-95	-0.027060122385499596
1105	96-97	-0.032165452953343276
1105	98-99	0.013044300191388913
1105	100	0.019163030409607984
1106	1	0.0846566007353644
1106	2	0.09018643365061507
1106	3	0.06295379266875045
1106	4	0.04078065770824679
1106	5	0.057617111800759346
1106	6	0.10617815505491279
1106	7	0.07966354799906838
1106	8	0.09205568255955399
1106	9	0.11283858632081234
1106	10-11	0.09936610528348666
1106	12-13	0.10880423216238455
1106	14-15	0.11581976070646505
1106	16-17	0.1569018258764885
1106	18-19	0.1447857635662544
1106	20-21	0.14634190950423687
1106	22-23	0.12147356788184283
1106	24-25	0.12353076635632476
1106	26-27	0.0988990364042408
1106	28-29	0.12580872151248457
1106	30-31	0.1389718687222512
1106	32-33	0.10953760140465363
1106	34-35	0.13868948783697022
1106	36-37	0.12729509473584244
1106	38-39	0.06484720756144213
1106	40-41	0.1449174806493545
1106	42-43	0.13060969333036354
1106	44-45	0.13153579991489295
1106	46-47	0.16457924776853616
1106	48-49	0.145811603697112
1106	50-51	0.19053158328986797
1106	52-53	0.1958143033070101
1106	54-55	0.1473483289654567
1106	56-57	0.16932687033086324
1106	58-59	0.12203434908414579
1106	60-61	0.10633283325095988
1106	62-63	0.11096177735732482
1106	64-65	0.191723806820157
1106	66-67	0.17825480683895023
1106	68-69	0.1934863659044197
1106	70-71	0.18287253702863637
1106	72-73	0.18780619871870385
1106	74-75	0.16876792623648385
1106	76-77	0.19505158919842103
1106	78-79	0.15074670480485963
1106	80-81	0.17133199388106846
1106	82-83	0.20328868287075608
1106	84-85	0.16834611613060702
1106	86-87	0.1527341141082772
1106	88-89	0.17766119116831902
1106	90-91	0.17193390506583484
1106	92-93	0.16593824053357054
1106	94-95	0.16540426734276537
1106	96-97	0.2170149221024218
1106	98-99	0.21078927133235226
1106	100	0.22244486790789608
1107	1	-0.09028321082760016
1107	2	-0.039061675116471406
1107	3	-0.02811280617995493
1107	4	-0.11854132419081509
1107	5	-0.10973290152062276
1107	6	-0.12780800685238347
1107	7	-0.07636393442074052
1107	8	-0.04897870275947014
1107	9	-0.03316411025033972
1107	10-11	-0.034834490545712526
1107	12-13	0.008384164505898184
1107	14-15	-0.04270891256437892
1107	16-17	-0.021838213361050407
1107	18-19	-0.011190936121302286
1107	20-21	-0.03080658760138988
1107	22-23	-0.04082287732690304
1107	24-25	0.017657489053540587
1107	26-27	-0.07188240408145674
1107	28-29	-0.0900514766128282
1107	30-31	-0.02800393479943608
1107	32-33	-0.03995053448961272
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2216	40-41	-0.8539230167424137
2216	42-43	-0.798285917137953
2216	44-45	-0.8839838958243433
2216	46-47	-0.8542266077915954
2216	48-49	-0.9827087472206557
2216	50-51	-0.9044492907497741
2216	52-53	-0.9269269852831528
2216	54-55	-0.9479688250933975
2216	56-57	-0.9524514445372176
2216	58-59	-0.9653971399619081
2216	60-61	-0.9484710910431033
2216	62-63	-1.0135102183250453
2216	64-65	-0.9163411728097586
2216	66-67	-0.8505365310527253
2216	68-69	-0.9038347056274887
2216	70-71	-0.8678645264851852
2216	72-73	-0.9525014440384183
2216	74-75	-0.9285751326242462
2216	76-77	-0.8631958331710905
2216	78-79	-0.8777328900492734
2216	80-81	-0.8385942295835633
2216	82-83	-0.8602939566143064
2216	84-85	-0.8826491888344705
2216	86-87	-0.8302991525009205
2216	88-89	-0.8160558610798745
2216	90-91	-0.8571536174979073
2216	92-93	-1.0661884188723434
2216	94-95	-1.0173821681970452
2216	96-97	-1.208587660931677
2216	98-99	-1.1594242283721385
2216	100	-1.180553671881249
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66.0
3	374.0
4	190.0
5	253.0
6	333.0
7	577.0
8	1004.0
9	1721.0
10	2415.0
11	3564.0
12	5587.0
13	8252.0
14	9131.0
15	12307.0
16	16092.0
17	24694.0
18	29038.0
19	14874.0
20	8910.0
21	8284.0
22	8025.0
23	8397.0
24	9518.0
25	11506.0
26	14383.0
27	18199.0
28	23004.0
29	29096.0
30	36650.0
31	46927.0
32	58728.0
33	77268.0
34	107694.0
35	175403.0
36	322116.0
37	719166.0
38	1269166.0
39	746543.0
40	5513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.84145555937456	21.752496073928537	15.137372079959901	24.268676286736998
2	13.549161296782659	22.09898491982202	40.28369989006427	24.06815389333105
3	15.300596747549841	28.213733929081307	31.895576349657297	24.590092973711556
4	10.67028991648463	18.807738372922316	40.0887854403143	30.433186270278757
5	11.89050338881576	38.53664489508126	35.93693611002752	13.63591560607546
6	28.057157191402897	39.98434928270588	18.516269236144865	13.442224289746354
7	24.537466805459655	33.95738895344107	24.165938281623212	17.339205959476065
8	24.430842708465885	35.37101222226626	21.46901877668862	18.729126292579235
9	25.38941654793469	15.941254268614497	22.208633813893623	36.460695369557186
10-11	22.938952815251653	27.235168585500585	29.867485204570155	19.95839339467761
12-13	24.31022892498712	25.43315876429738	29.683546251233388	20.573066059482112
14-15	21.79332395993917	26.628227406330378	27.9371692280092	23.641279405721246
16-17	20.377236524529017	29.75629783612275	28.288241257814928	21.5782243815333
18-19	20.15114598087911	28.00514893474991	31.59427927429903	20.249425810071948
20-21	21.667847032882673	27.100604229292134	31.14847372911586	20.08307500870933
22-23	21.24422729637925	27.09387422289086	31.184053367871662	20.477845112858223
24-25	20.92800883241783	27.288382275346297	31.13251273447583	20.65109615776004
26-27	20.765334320394928	27.261661588158287	31.194566606644177	20.778437484802613
28-29	20.64333016248511	27.20419584577161	31.41795586679934	20.73451812494394
30-31	21.027864658675956	27.37862399171402	31.077744558120834	20.51576679148919
32-33	20.611387714198617	27.282723008685945	31.337297190452635	20.768592086662803
34-35	20.946780064285537	27.138109600429335	31.12620170305046	20.788908632234666
36-37	21.123631163447833	27.43805064427956	30.65519791510749	20.783120277165118
38-39	21.074259965618154	27.411784876811417	30.687447887772016	20.82650726979841
40-41	20.991346585239636	27.305696489127524	30.786020137192004	20.916936788440836
42-43	20.976085610492234	27.438033424420993	30.60394130250182	20.981939662584953
44-45	21.21319072056545	27.406277546781205	30.285869176770987	21.09466255588236
46-47	21.37893807686641	27.5792569954118	29.85022823667772	21.191576691044073
48-49	21.4005914135514	27.64897467456609	29.47097431992657	21.47945959195594
50-51	21.467444342240704	27.747163737562524	29.161373766031083	21.624018154165686
52-53	21.502670623764413	27.8662350417494	29.077419340539045	21.55367499394714
54-55	21.606302674341876	28.058522896100037	29.002096119538656	21.33307831001943
56-57	21.49562405262558	27.790805647335905	29.101164789758172	21.61240551028034
58-59	21.4368179732692	27.434291988178693	29.364870032499862	21.764020006052245
60-61	21.51824087773582	27.82405961160461	29.104360464964675	21.553339045694894
62-63	21.389813422171972	27.661860542252242	29.31628112672648	21.632044908849306
64-65	21.440204976938528	27.567205254385435	29.360505746071414	21.632084022604623
66-67	21.460147626782263	27.597543221655823	29.173708278693205	21.76860087286871
68-69	21.74880236206808	27.652394969470873	29.02019833663825	21.578604331822795
70-71	21.701773243276083	27.591140719926027	29.029824553168876	21.677261483629014
72-73	21.57532639159064	27.72385868550526	29.003636018486745	21.697178904417356
74-75	21.711300199197005	27.667457561441676	28.871518583158817	21.749723656202498
76-77	21.72299396146367	27.666652976796975	28.74943040363603	21.860922658103327
78-79	21.757991483764744	27.790387806964468	28.801754304305145	21.649866404965646
80-81	21.56799191015326	27.738351451219263	28.929833278595268	21.76382336003221
82-83	21.505837863956277	27.793137356266616	28.987633775554468	21.713391004222643
84-85	21.523784689400706	27.639015104597874	28.831333076918064	22.00586712908336
86-87	21.603897408537797	27.77820151117672	28.703596140610127	21.914304939675358
88-89	21.364228294394547	27.67806946085799	28.86736787427495	22.09033437047251
90-91	21.34790280770385	27.629075288018267	28.829363273690955	22.193658630586928
92-93	21.358737339317567	27.79054589945981	28.79003623214388	22.060680529078745
94-95	21.205753901278733	27.56254777631447	28.938244872101265	22.293453450305527
96-97	21.230687270388447	27.709584162293748	28.75725887698773	22.302469690330074
98-99	21.07151574131732	27.65384069784786	28.8415974858118	22.433046075023025
100	21.230555882963444	27.615105313499715	28.740890092822745	22.413448710714096
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1063.0
1	690.5
2	897.0
3	1289.0
4	726.0
5	510.0
6	1240.5
7	3501.5
8	6157.0
9	6549.5
10	4309.5
11	2391.5
12	2241.5
13	2577.0
14	2898.5
15	3165.5
16	3601.0
17	4197.0
18	4882.5
19	5640.0
20	6867.5
21	8420.5
22	10523.5
23	13655.5
24	18296.5
25	24430.0
26	32049.0
27	41300.5
28	52565.0
29	64907.5
30	75563.0
31	84614.0
32	93421.0
33	102065.0
34	110074.5
35	117920.0
36	124634.5
37	130034.5
38	134124.0
39	135766.0
40	137187.0
41	136875.0
42	133447.0
43	130585.0
44	128336.5
45	125605.0
46	122167.0
47	117214.0
48	112811.5
49	112051.5
50	110886.5
51	105567.0
52	99581.0
53	94934.5
54	90679.0
55	86479.0
56	79439.5
57	70208.0
58	64559.5
59	60968.5
60	53812.5
61	44275.5
62	36723.0
63	30537.0
64	24606.5
65	19871.0
66	16021.0
67	12368.5
68	9550.0
69	7332.5
70	5393.0
71	4082.5
72	3092.0
73	2316.5
74	1849.5
75	1387.5
76	904.0
77	679.0
78	521.5
79	353.0
80	207.0
81	91.0
82	51.0
83	35.5
84	19.5
85	11.5
86	7.5
87	4.0
88	3.0
89	3.5
90	3.0
91	3.5
92	2.5
93	0.0
94	5.0
95	11.0
96	9.0
97	7.5
98	5.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009022239559756432
2	0.0
3	3.3898587941281387E-4
4	0.00273796287218042
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.911375531686314E-5
24-25	1.1734126595058942E-4
26-27	4.3025130848549457E-4
28-29	8.344267800930803E-4
30-31	0.001473284783601845
32-33	0.003937451368564222
34-35	0.005267319049337569
36-37	0.003702768836663044
38-39	0.0024902424218402864
40-41	0.0032203658544217315
42-43	4.5632714536340325E-4
44-45	3.7809963472967703E-4
46-47	0.0034680863047618646
48-49	0.0022946736452559707
50-51	9.25692209165761E-4
52-53	6.51895921947719E-5
54-55	6.388580035087645E-4
56-57	7.040475957035365E-4
58-59	7.431613510203996E-4
60-61	2.607583687790876E-4
62-63	0.0
64-65	0.0
66-67	7.040475957035365E-4
68-69	6.127821666308559E-4
70-71	0.0019296119289652484
72-73	1.0430334751163504E-4
74-75	0.001851384418331522
76-77	4.041754716075857E-4
78-79	0.0014993606204797537
80-81	9.778438829215785E-4
82-83	0.0012125264148227573
84-85	0.0011212609857500766
86-87	2.4772045034013323E-4
88-89	0.0015645502126745256
90-91	0.0014341710282849817
92-93	0.002959607485642644
94-95	0.001029995556677396
96-97	0.0024641665849623774
98-99	0.0023989769927676057
100	0.0034420104678839565
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3834968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.408116636480464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43840583159071	39.02859406004524
2	16.811617610552716	18.630001388268276
3	5.978809223420334	9.938236763956173
4	2.6303761683585383	5.829767581369138
5	1.3548966540079528	3.7536135917824875
6	0.7668126309673216	2.5492586216978284
7	0.4975514782574465	1.9297873237959304
8	0.3113727275067999	1.3802061122492655
9	0.2075544632434575	1.035018171703403
>10	0.9146949605285176	8.64060276124428
>50	0.05378146679690314	2.0574525203024874
>100	0.03196131368749556	3.298133690784814
>500	0.0015534901550846053	0.5814040002691532
>1k	4.707545591013575E-4	0.3818120810541829
>5k	4.707545591013483E-5	0.22940546883942428
>10k+	9.415091182026966E-5	0.736705862637942
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15952	0.4159617498764005	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12292	0.32052418690325446	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8795	0.22933698534120753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.607583687790876E-5	0.0
8	0.0	0.0	0.0	2.607583687790876E-5	0.0
9	2.607583687790876E-5	0.0	0.0	2.607583687790876E-5	0.0
10-11	3.9113755316863135E-5	0.0	0.0	2.607583687790876E-5	0.0
12-13	5.215167375581752E-5	0.0	0.0	2.607583687790876E-5	0.0
14-15	5.215167375581752E-5	0.0	0.0	2.607583687790876E-5	0.0
16-17	5.215167375581752E-5	0.0	0.0	2.607583687790876E-5	0.0
18-19	7.822751063372627E-5	0.0	0.0	3.9113755316863135E-5	0.0
20-21	1.0430334751163504E-4	0.0	0.0	9.126542907268067E-5	0.0
22-23	1.0430334751163504E-4	0.0	0.0	2.8683420565699634E-4	0.0
24-25	1.303791843895438E-4	0.0	0.0	4.302513084854945E-4	0.0
26-27	1.303791843895438E-4	0.0	0.0	8.213888616541259E-4	0.0
28-29	1.303791843895438E-4	0.0	0.0	0.0021382186239885183	0.0
30-31	1.303791843895438E-4	0.0	0.0	0.00535858447841025	0.0
32-33	1.303791843895438E-4	0.0	0.0	0.012177415821983392	0.0
34-35	1.825308581453613E-4	0.0	0.0	0.022985850207876572	0.0
36-37	1.825308581453613E-4	0.0	0.0	0.039426665359398046	0.0
38-39	1.825308581453613E-4	0.0	0.0	0.06020910735109133	0.0
40-41	1.825308581453613E-4	0.0	0.0	0.08117408020092998	0.0
42-43	1.825308581453613E-4	0.0	0.0	0.10148715712882089	0.0
44-45	2.0860669502327007E-4	0.0	0.0	0.12932311299598848	0.0
46-47	2.3468253190117884E-4	0.0	0.0	0.16276537379190648	0.0
48-49	2.3468253190117884E-4	0.0	0.0	0.1973158576551356	0.0
50-51	2.3468253190117884E-4	0.0	0.0	0.23352215716011188	0.0
52-53	2.3468253190117884E-4	0.0	0.0	0.26965022915445447	0.0
54-55	2.3468253190117884E-4	0.0	0.0	0.310941316850623	0.0
56-57	2.8683420565699634E-4	0.0	0.0	0.360381103571138	0.0
58-59	3.3898587941281387E-4	0.0	0.0	0.42002958042935434	0.0
60-61	4.041754716075858E-4	0.0	0.0	0.4831070298370156	0.0
62-63	4.1721339004654014E-4	0.0	0.0	0.5479576361523746	0.0
64-65	4.1721339004654014E-4	0.0	0.0	0.6172932864107341	0.0
66-67	4.1721339004654014E-4	0.0	0.0	0.6955859866366552	0.0
68-69	4.1721339004654014E-4	0.0	0.0	0.7874641978759667	0.0
70-71	4.1721339004654014E-4	0.0	0.0	0.8938927260931513	0.0
72-73	4.1721339004654014E-4	0.0	0.0	1.004858449927092	0.0
74-75	4.693650638023577E-4	0.0	0.0	1.1221345262854867	0.0
76-77	4.693650638023577E-4	0.0	0.0	1.2511056154836233	0.0
78-79	4.824029822413121E-4	0.0	0.0	1.4005592745493574	0.0
80-81	4.954409006802665E-4	0.0	0.0	1.5679661473055315	0.0
82-83	4.954409006802665E-4	0.0	0.0	1.7509402946778172	0.0
84-85	4.954409006802665E-4	0.0	0.0	1.9349705134436586	0.0
86-87	5.084788191192208E-4	0.0	0.0	2.1372798938609137	0.0
88	5.215167375581752E-4	0.0	0.0	2.3044520840851868	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6995	0.0	58.257645	1
GTATCAA	14275	0.0	48.138527	1
ATCAACG	18785	0.0	36.177536	3
TCAACGC	18850	0.0	36.02785	4
TATCAAC	19395	0.0	35.1124	2
CAACGCA	19750	0.0	34.36183	5
AACGCAG	20325	0.0	33.38973	6
ACGCAGA	22810	0.0	29.628511	7
CGCAGAG	22985	0.0	29.32114	8
GTGGTAT	2800	0.0	27.026474	1
GCAGAGT	25415	0.0	26.40671	9
TGGTATC	2790	0.0	25.43634	2
GTACATG	14640	0.0	25.267088	1
TACATGG	14480	0.0	24.635145	2
ACATGGG	14825	0.0	23.3327	3
AGAGTAC	24135	0.0	22.130863	10-11
GAGTACT	16670	0.0	21.595804	12-13
AGTACTT	17585	0.0	21.39416	12-13
CAGAGTA	25070	0.0	20.90243	10-11
CATGGGT	3775	0.0	19.421803	4
>>END_MODULE