FastQCFastQC Report
Wed 25 May 2016
SRR2431346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786746
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA286500.7565862616610672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.051.3692051
GTATCAA91700.039.8760381
TCAACGC106300.033.204944
ATCAACG107500.032.9654433
CAACGCA116750.030.3536195
TATCAAC120450.029.8504352
AACGCAG121100.029.1468626
ACGCAGA136600.025.4954997
GTACATG218200.025.4172781
TACATGG211700.025.3314932
CGCAGAG138800.025.023678
GTGGTAT21950.023.3396171
ACATGGG221950.023.1028543
GCAGAGT153950.021.889489
TGGTATC23100.020.5496962
CATGGGG133250.019.6111764
CAGAGTA149500.018.84707810-11
TAGACCG1801.3385688E-418.27775
AGAGTAC142800.017.92114310-11
CATGGGA60400.017.7416484
Produced by FastQC (version 0.11.5)