##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431346_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3786746
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.062351158488053	33.0	31.0	34.0	28.0	34.0
2	31.17879176475	34.0	31.0	34.0	27.0	34.0
3	31.240517320147696	34.0	31.0	34.0	27.0	34.0
4	34.441144454896104	37.0	35.0	37.0	31.0	37.0
5	34.57710736341968	37.0	35.0	37.0	32.0	37.0
6	34.62020769283179	37.0	35.0	37.0	32.0	37.0
7	34.538621285927285	37.0	35.0	37.0	31.0	37.0
8	34.511672026589586	37.0	35.0	37.0	31.0	37.0
9	35.98945427023624	39.0	37.0	39.0	31.0	39.0
10-11	36.01069995188481	39.0	37.0	39.0	31.0	39.0
12-13	35.94921550059074	39.0	37.0	39.0	31.0	39.0
14-15	37.31722988550064	41.0	38.0	41.0	31.0	41.0
16-17	37.325189885986546	41.0	38.0	41.0	31.0	41.0
18-19	37.348301285589265	41.0	38.0	41.0	31.0	41.0
20-21	37.39152084665832	41.0	38.0	41.0	31.0	41.0
22-23	37.400038317859185	41.0	38.0	41.0	31.0	41.0
24-25	37.42017090663066	41.0	38.0	41.0	30.5	41.0
26-27	37.41874554036632	41.0	38.0	41.0	30.0	41.0
28-29	37.38872702314863	40.5	38.0	41.0	30.0	41.0
30-31	37.31655701227386	40.0	38.0	41.0	30.0	41.0
32-33	37.23969418598448	40.0	38.0	41.0	30.0	41.0
34-35	37.16565555228685	40.0	38.0	41.0	30.0	41.0
36-37	37.06195992548748	40.0	38.0	41.0	30.0	41.0
38-39	36.95461208647213	40.0	38.0	41.0	30.0	41.0
40-41	36.85586015011305	40.0	37.0	41.0	30.0	41.0
42-43	36.7294355100659	40.0	37.0	41.0	29.5	41.0
44-45	36.58472947485784	40.0	37.0	41.0	29.0	41.0
46-47	36.44828884746957	40.0	36.5	41.0	29.0	41.0
48-49	36.30291099535063	40.0	36.0	41.0	29.0	41.0
50-51	36.15575655193139	40.0	36.0	41.0	28.5	41.0
52-53	35.975980300764824	40.0	35.0	41.0	28.0	41.0
54-55	35.80103695890878	39.5	35.0	41.0	28.0	41.0
56-57	35.58724773195773	39.0	35.0	41.0	28.0	41.0
58-59	35.35081584558352	39.0	35.0	41.0	28.0	41.0
60-61	35.15876018618624	39.0	35.0	41.0	27.5	41.0
62-63	34.97694590553473	38.0	35.0	41.0	27.0	41.0
64-65	34.71365071752898	38.0	34.0	40.0	27.0	41.0
66-67	34.395585154113846	37.0	34.0	40.0	26.0	41.0
68-69	34.07534120323888	37.0	34.0	40.0	26.0	41.0
70-71	33.82248650952559	36.0	34.0	39.0	26.0	41.0
72-73	33.57637850545032	36.0	34.0	39.0	26.0	41.0
74-75	33.241434598465275	35.0	34.0	39.0	25.5	41.0
76-77	32.9042688630291	35.0	33.0	37.5	25.0	40.0
78-79	32.5785854134394	35.0	33.0	37.0	24.5	39.0
80-81	32.25384366947242	35.0	33.0	37.0	24.0	39.0
82-83	31.93581468099524	35.0	33.0	36.0	23.5	39.0
84-85	31.662661556914564	35.0	33.0	36.0	23.0	37.0
86-87	31.4125796924325	35.0	33.0	35.5	22.5	37.0
88-89	31.17635207114499	35.0	32.0	35.0	20.5	36.5
90-91	30.974988684215948	35.0	32.0	35.0	20.0	36.0
92-93	30.780901861387058	35.0	32.0	35.0	19.0	36.0
94-95	30.5736913698463	35.0	32.0	35.0	17.0	36.0
96-97	30.393906007955117	35.0	32.0	35.0	10.0	35.5
98-99	30.275009335191747	35.0	32.0	35.0	2.0	35.0
100	30.237055244793286	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33284783674478646
1101	2	0.3581026294902401
1101	3	0.36420405128567523
1101	4	0.38982615627993056
1101	5	0.3545987032407538
1101	6	0.3085341364680261
1101	7	0.30247791474868535
1101	8	0.3227647305026622
1101	9	0.4559683399572023
1101	10-11	0.39397047486239956
1101	12-13	0.4758823724899983
1101	14-15	0.5877791823409169
1101	16-17	0.5596460976120525
1101	18-19	0.5272359614860491
1101	20-21	0.5171532309258922
1101	22-23	0.48677540686897913
1101	24-25	0.48520142765757157
1101	26-27	0.4606131238921378
1101	28-29	0.48560428459542493
1101	30-31	0.5559015050066947
1101	32-33	0.5436873352768998
1101	34-35	0.5828901620371951
1101	36-37	0.5308568140205594
1101	38-39	0.4936540525082904
1101	40-41	0.509411560344617
1101	42-43	0.5134489025237201
1101	44-45	0.5108548193900191
1101	46-47	0.5385464761239191
1101	48-49	0.48479169909231246
1101	50-51	0.5424348833071448
1101	52-53	0.519113471725376
1101	54-55	0.6038479145358622
1101	56-57	0.605836982633889
1101	58-59	0.6251969339274694
1101	60-61	0.6347947736190633
1101	62-63	0.622670162927264
1101	64-65	0.6791217255671
1101	66-67	0.5640772950019368
1101	68-69	0.6579764210880654
1101	70-71	0.63943509289372
1101	72-73	0.6258319636406426
1101	74-75	0.672031911098216
1101	76-77	0.6378378938691469
1101	78-79	0.5717359849893313
1101	80-81	0.5637659127593508
1101	82-83	0.5900029102486464
1101	84-85	0.5988344996650135
1101	86-87	0.5978318488725414
1101	88-89	0.5888021246279038
1101	90-91	0.5646210450262004
1101	92-93	0.5935096726858049
1101	94-95	0.5714047806711271
1101	96-97	0.5519800022152026
1101	98-99	0.5903557923939609
1101	100	0.6468294916656845
1102	1	0.06760341939975945
1102	2	0.16859845843408294
1102	3	0.11568429071048314
1102	4	0.07070996378678274
1102	5	0.05180997057133396
1102	6	0.12719443670016517
1102	7	0.14100934567392187
1102	8	0.1242671931381949
1102	9	0.11060112897557417
1102	10-11	0.12445218510573142
1102	12-13	0.1374315707727547
1102	14-15	0.1814407392965265
1102	16-17	0.15803156055824275
1102	18-19	0.16980914166909145
1102	20-21	0.14314979455569699
1102	22-23	0.13838421181717564
1102	24-25	0.2060815716798814
1102	26-27	0.2626545852789022
1102	28-29	0.2545797963892795
1102	30-31	0.26144727198231266
1102	32-33	0.31581380541733495
1102	34-35	0.3353995585314635
1102	36-37	0.3329510035460359
1102	38-39	0.2817324662665044
1102	40-41	0.3208941613246097
1102	42-43	0.3413692114281588
1102	44-45	0.2999294441721716
1102	46-47	0.25898004029656363
1102	48-49	0.21244339465638262
1102	50-51	0.2256331957173856
1102	52-53	0.26934351540872115
1102	54-55	0.283213274607931
1102	56-57	0.2997812933291968
1102	58-59	0.3679387501261502
1102	60-61	0.36546745158285887
1102	62-63	0.3197583075224202
1102	64-65	0.3584961087328651
1102	66-67	0.36964539368239
1102	68-69	0.3287989004895522
1102	70-71	0.311003324660291
1102	72-73	0.3141309278473514
1102	74-75	0.3164931419928507
1102	76-77	0.2919159705723189
1102	78-79	0.2932058306781542
1102	80-81	0.243477242474043
1102	82-83	0.25125758318048597
1102	84-85	0.28853748618244524
1102	86-87	0.23208058319830016
1102	88-89	0.23880810988644896
1102	90-91	0.19649625365133616
1102	92-93	0.2258028367972571
1102	94-95	0.19065134692287344
1102	96-97	0.19951503406003468
1102	98-99	0.18557494507664174
1102	100	0.21189489372497405
1103	1	0.04342940670500539
1103	2	0.04910860355925095
1103	3	0.10168147109665782
1103	4	0.053439098063208235
1103	5	-0.02991264986509634
1103	6	0.04810970357461031
1103	7	0.06564244604594194
1103	8	0.09791838193128655
1103	9	0.07378717420507996
1103	10-11	0.11897949254569795
1103	12-13	0.08637884790262262
1103	14-15	0.10619754635595768
1103	16-17	0.06243032644405844
1103	18-19	0.052176723627354704
1103	20-21	0.04605848147536307
1103	22-23	0.081422114276684
1103	24-25	0.11719118464876743
1103	26-27	0.1944827147888546
1103	28-29	0.15261462169397078
1103	30-31	0.07739356323870794
1103	32-33	0.05935534636545725
1103	34-35	0.0912454751302576
1103	36-37	0.09706895346839417
1103	38-39	0.0485052422937855
1103	40-41	0.059928271348603346
1103	42-43	0.02787264422633484
1103	44-45	0.013509967169383685
1103	46-47	-0.041342246113984515
1103	48-49	-0.06319480739511363
1103	50-51	-0.11280187652996432
1103	52-53	-0.1491098314794499
1103	54-55	-0.1722862311715474
1103	56-57	-0.2012344102523258
1103	58-59	-0.19409223695478772
1103	60-61	-0.16867397158537045
1103	62-63	-0.17135704537970042
1103	64-65	-0.1333917244986793
1103	66-67	-0.16247580192687394
1103	68-69	-0.179339013245837
1103	70-71	-0.10726348152973486
1103	72-73	-0.10259972585672728
1103	74-75	-0.12540050035353545
1103	76-77	-0.20494537787408262
1103	78-79	-0.25332833515470554
1103	80-81	-0.21588803488105412
1103	82-83	-0.24695483913909655
1103	84-85	-0.23307347301046022
1103	86-87	-0.23327576730535782
1103	88-89	-0.19484491614174004
1103	90-91	-0.22064604840851487
1103	92-93	-0.1647023379508319
1103	94-95	-0.15554757699655042
1103	96-97	-0.16705302148551482
1103	98-99	-0.20641797286706165
1103	100	-0.2674269753850069
1104	1	0.16347331987985214
1104	2	0.21712577074468697
1104	3	0.09976468029246277
1104	4	0.15563908214678435
1104	5	0.09325090263126867
1104	6	0.17906701294372596
1104	7	0.16247963334647153
1104	8	0.15977810398130998
1104	9	0.19360204959993155
1104	10-11	0.2240091997757716
1104	12-13	0.2403792300498253
1104	14-15	0.21475176294254084
1104	16-17	0.2255257911793649
1104	18-19	0.20725071601858502
1104	20-21	0.20466857082271872
1104	22-23	0.18276336327446785
1104	24-25	0.2936595048995372
1104	26-27	0.24062528464772726
1104	28-29	0.2941235714443877
1104	30-31	0.3596847845323836
1104	32-33	0.34245082710765473
1104	34-35	0.34946010023296026
1104	36-37	0.3710553455899088
1104	38-39	0.28360288452347504
1104	40-41	0.2886651913235596
1104	42-43	0.25707776586185105
1104	44-45	0.2661614135297441
1104	46-47	0.28354474782152295
1104	48-49	0.23903862065844095
1104	50-51	0.23883881448488609
1104	52-53	0.28012335789738074
1104	54-55	0.2245250632374436
1104	56-57	0.15296806241617134
1104	58-59	0.1790365266445022
1104	60-61	0.17657963799540966
1104	62-63	0.17413210913161947
1104	64-65	0.2311814932458489
1104	66-67	0.26667232472538416
1104	68-69	0.22419648171187134
1104	70-71	0.22396471320888622
1104	72-73	0.16664361467495326
1104	74-75	0.14771052343107272
1104	76-77	0.18519666139103919
1104	78-79	0.09321722218917472
1104	80-81	0.1385145413659572
1104	82-83	0.1671851015525796
1104	84-85	0.15051264688250043
1104	86-87	0.123811948074799
1104	88-89	0.11245883489648989
1104	90-91	0.10557856513546682
1104	92-93	0.1112251162984208
1104	94-95	0.19654537837967112
1104	96-97	0.20756229543516014
1104	98-99	0.236218850499327
1104	100	0.23580650427338057
1105	1	0.1336925573679011
1105	2	0.2331718676957948
1105	3	0.22413679442499657
1105	4	0.24639271785548544
1105	5	0.18971085606993654
1105	6	0.19993517228319746
1105	7	0.18959380237667744
1105	8	0.21714787179795536
1105	9	0.18740524319499485
1105	10-11	0.15865361935107103
1105	12-13	0.22377768345227622
1105	14-15	0.26714440081140367
1105	16-17	0.2965886719959485
1105	18-19	0.24741305597805763
1105	20-21	0.25737950341379445
1105	22-23	0.21461417329673083
1105	24-25	0.2168995889985581
1105	26-27	0.22816553518159566
1105	28-29	0.2490178805404213
1105	30-31	0.23648414378066462
1105	32-33	0.23093386102857494
1105	34-35	0.27742261182135763
1105	36-37	0.251998344460695
1105	38-39	0.34416323273954674
1105	40-41	0.2466119817251453
1105	42-43	0.30474981873243223
1105	44-45	0.2938948701356736
1105	46-47	0.25225651174458363
1105	48-49	0.15169912093443116
1105	50-51	0.23025601721702316
1105	52-53	0.2360758885887222
1105	54-55	0.21225923157135895
1105	56-57	0.19974866545613423
1105	58-59	0.21537067900314355
1105	60-61	0.16571527524043006
1105	62-63	0.25112626706616936
1105	64-65	0.27515422760650665
1105	66-67	0.2766909138811755
1105	68-69	0.29425029157633276
1105	70-71	0.3114565255294508
1105	72-73	0.3267541599287753
1105	74-75	0.32637325182275845
1105	76-77	0.2394953650634477
1105	78-79	0.21773089227981046
1105	80-81	0.24705756890239172
1105	82-83	0.2715539041144588
1105	84-85	0.24126451029024665
1105	86-87	0.26156726820639875
1105	88-89	0.25875668070504076
1105	90-91	0.34202670978555005
1105	92-93	0.3425634716424426
1105	94-95	0.2846191897658592
1105	96-97	0.3012893257129363
1105	98-99	0.29592707290884945
1105	100	0.3257145243106976
1106	1	0.19522818398034758
1106	2	0.26449109114772895
1106	3	0.19728340367110064
1106	4	0.2132085484952171
1106	5	0.21320439352088272
1106	6	0.25753631440664293
1106	7	0.21914269018980548
1106	8	0.2219864461602583
1106	9	0.279586324840821
1106	10-11	0.22655298346584374
1106	12-13	0.2624930850792211
1106	14-15	0.28940990999117844
1106	16-17	0.2528092271957121
1106	18-19	0.2257279588183536
1106	20-21	0.25184973671066757
1106	22-23	0.2802469945209012
1106	24-25	0.2515673157779048
1106	26-27	0.3099882253101285
1106	28-29	0.3201324631192577
1106	30-31	0.3484346681788466
1106	32-33	0.3094203259954895
1106	34-35	0.2992646057016799
1106	36-37	0.3726796869117024
1106	38-39	0.2824687878898118
1106	40-41	0.2540692739004129
1106	42-43	0.2329461607379102
1106	44-45	0.23165473083808763
1106	46-47	0.23334383744673914
1106	48-49	0.23172620259484233
1106	50-51	0.2268634818467845
1106	52-53	0.2160838430163352
1106	54-55	0.24747454174714534
1106	56-57	0.24257360499674974
1106	58-59	0.26671580254559046
1106	60-61	0.2830817571896347
1106	62-63	0.28908286502026215
1106	64-65	0.2875122991749919
1106	66-67	0.2589640433400717
1106	68-69	0.2698145433155972
1106	70-71	0.28800684099688567
1106	72-73	0.11726510488768582
1106	74-75	0.173257310633943
1106	76-77	0.22830832762787168
1106	78-79	0.21945248978837384
1106	80-81	0.18100121609473518
1106	82-83	0.1914518018840461
1106	84-85	0.18271777027679192
1106	86-87	0.23202754830751005
1106	88-89	0.22063934299698928
1106	90-91	0.16398154880283755
1106	92-93	0.19982499058117043
1106	94-95	0.2151923176093824
1106	96-97	0.1809361717941158
1106	98-99	0.23296932855836872
1106	100	0.24601359567752112
1107	1	0.09549006904845214
1107	2	0.14718363367245502
1107	3	0.13709342254337997
1107	4	0.06905474475878748
1107	5	0.12086516370943912
1107	6	0.04173940381800634
1107	7	0.0956628070748593
1107	8	0.10235494872885909
1107	9	0.1336899012068642
1107	10-11	0.1286688662537827
1107	12-13	0.10082346752269444
1107	14-15	0.15924161656391078
1107	16-17	0.17826613768652066
1107	18-19	0.22585827306726003
1107	20-21	0.19168841651631396
1107	22-23	0.23202328259406357
1107	24-25	0.2266769866856535
1107	26-27	0.292931132877321
1107	28-29	0.27555854604487706
1107	30-31	0.31954922172629807
1107	32-33	0.30288485698471845
1107	34-35	0.28249088598205674
1107	36-37	0.30236810522038127
1107	38-39	0.2803808161937553
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2216	54-55	-0.9944978402833087
2216	56-57	-0.8943544186800096
2216	58-59	-0.9007646037234949
2216	60-61	-0.8416840660194254
2216	62-63	-0.9267026089397419
2216	64-65	-0.9532468878207965
2216	66-67	-0.9685458176445465
2216	68-69	-1.0263411896030945
2216	70-71	-1.028382805679172
2216	72-73	-0.9380692259052452
2216	74-75	-0.9750181651504022
2216	76-77	-0.8709082736788645
2216	78-79	-0.8591190785680425
2216	80-81	-0.8179117844564985
2216	82-83	-0.7501414183968116
2216	84-85	-0.7467757042462999
2216	86-87	-0.771101328001496
2216	88-89	-0.7431969211052518
2216	90-91	-0.7319356280556377
2216	92-93	-0.8248739999096202
2216	94-95	-0.837971734079968
2216	96-97	-0.8948153640268472
2216	98-99	-0.9422052760477087
2216	100	-0.9843895336502086
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74929.0
3	16294.0
4	11821.0
5	10569.0
6	7159.0
7	6284.0
8	7096.0
9	7553.0
10	7546.0
11	7974.0
12	9205.0
13	10122.0
14	10861.0
15	11211.0
16	11031.0
17	10890.0
18	10409.0
19	9862.0
20	9610.0
21	10025.0
22	12437.0
23	20321.0
24	33254.0
25	41455.0
26	38741.0
27	29542.0
28	31402.0
29	37642.0
30	44220.0
31	53184.0
32	68806.0
33	92396.0
34	133743.0
35	221333.0
36	388224.0
37	733490.0
38	1050399.0
39	493699.0
40	2007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30734290923457	21.502943966400544	13.799750074470623	22.389963049894263
2	15.381960371728617	22.442015442830655	39.284281655386515	22.891742530054206
3	17.5481845225913	28.948899384563788	29.888730294751824	23.614185798093086
4	12.047199450503454	19.41588791833818	38.61876934217013	29.91814328898823
5	12.390034185032265	40.604397708322054	34.204785376358856	12.80078273028683
6	27.965778533865222	40.24310054067529	18.634099039122244	13.157021886337242
7	25.122228953301857	35.32323002387802	22.952133573257886	16.602407449562236
8	24.098817295905246	37.26730020973152	20.73431912253951	17.899563371823724
9	25.2184857394713	18.7412094711396	21.023353560022244	35.016951229366846
10-11	22.93372726874208	29.244422520021146	28.427124502145112	19.39472570909166
12-13	23.560545122382116	27.660648482892704	27.950567584939684	20.8282388097855
14-15	21.544526619952855	28.724279368090706	25.744742319659146	23.98645169229729
16-17	21.035725659972968	31.576464331117005	26.14676294633968	21.241047062570345
18-19	21.096384600393055	30.523726703613075	27.94664601216982	20.433242683824055
20-21	22.86358789314097	29.269047884384115	27.292562004422795	20.57480221805212
22-23	22.453116739279583	29.39884270030258	27.143713890501235	21.004326669916598
24-25	21.868472297851508	29.620325736133346	27.140029988808333	21.371171977206814
26-27	21.682705943308584	29.624498183928893	27.25453463210894	21.438261240653585
28-29	21.649854518893	29.453229765080625	27.548441326669387	21.348474389356983
30-31	21.78837714491545	29.626518388083067	27.313846769759575	21.271257697241907
32-33	21.422957864087003	29.471081503750185	27.573040283134915	21.532920349027897
34-35	21.93644622586252	29.235047716429886	27.42736111690618	21.401144940801416
36-37	22.04209101957195	29.36703438783589	27.15660094445204	21.434273648140117
38-39	21.77192502481022	29.32806953516291	27.325466244633255	21.57453919539362
40-41	21.66563323761351	29.278832010385695	27.50823530281672	21.547299449184077
42-43	21.68505624618076	29.324794955880325	27.481206819786696	21.50894197815222
44-45	21.613160745399878	29.319830271161575	27.413417747057768	21.653591236380787
46-47	21.842368091231894	29.226372722120786	27.395090666234285	21.53616852041304
48-49	21.727797428187685	29.07720771342995	27.425948294393127	21.769046563989242
50-51	21.728484033521127	29.237807341712386	27.19766522497152	21.836043399794967
52-53	21.640968261404385	29.215598299965194	27.322006810068594	21.82142662856183
54-55	21.735772613214618	29.336744529472004	27.300708840783088	21.626774016530288
56-57	21.700967004388463	29.271120904333163	27.247180032671853	21.78073205860652
58-59	21.875655245955233	28.855579434163264	27.317873974119205	21.9508913457623
60-61	21.965111998533835	29.154054694980864	27.22012514174439	21.660708164740917
62-63	21.586317117651937	29.149948266928916	27.417722234340513	21.846012381078637
64-65	21.868765672263955	29.079931566565538	27.313005323436705	21.7382974377338
66-67	21.759222826141496	29.000704034545755	27.277681154215255	21.962391985097497
68-69	21.9660098670468	29.099363939382254	27.212202772512335	21.722423421058608
70-71	21.96454422873887	29.020127043113003	27.315682118631667	21.699646609516453
72-73	21.856153013695664	29.03275001808941	27.314506967195584	21.796590001019347
74-75	21.813543871175938	28.991461270441693	27.397282521721817	21.797712336660553
76-77	21.812434739483454	29.0075040681366	27.239904656927084	21.940156535452868
78-79	21.892226201598948	29.12931049507942	27.249437907902983	21.729025395418653
80-81	21.72843121772625	29.17160274282986	27.366213630383445	21.733752409060443
82-83	21.690413553392336	29.200391787980273	27.443947858079582	21.665246800547806
84-85	21.797720822548925	28.880914523055196	27.25708047366624	22.06428418072964
86-87	21.91848705741985	29.050196121306037	27.20383279632037	21.82748402495374
88-89	21.709542240353922	29.042223980461713	27.318692996596432	21.929540782587935
90-91	21.65994631908625	28.97839757177515	27.275645431319024	22.08601067781958
92-93	21.712530231079267	28.992100174665524	27.379203763944325	21.916165830310884
94-95	21.733675358538214	28.877951957932325	27.4360746098046	21.952298073724855
96-97	21.778635171955287	29.09645486433652	27.110196498636334	22.01471346507186
98-99	21.451259261997933	29.058922251044294	27.418880974372417	22.070937512585356
100	21.540527514607337	29.05442181362031	27.278909318984585	22.126141352787773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24951.0
1	16228.0
2	5998.5
3	3700.5
4	2439.5
5	1865.5
6	1729.0
7	1885.0
8	2243.0
9	2348.0
10	1832.5
11	1296.0
12	1181.5
13	1168.0
14	1214.5
15	1374.0
16	1603.0
17	1910.5
18	2406.5
19	3133.0
20	4296.0
21	5810.5
22	7997.0
23	11581.5
24	16411.0
25	22245.0
26	29555.5
27	38493.0
28	49053.0
29	60517.5
30	70177.0
31	78239.5
32	85107.5
33	91962.5
34	99186.5
35	105885.5
36	111676.5
37	116978.0
38	124103.5
39	128455.0
40	130812.0
41	131638.0
42	128642.5
43	127662.0
44	126784.0
45	123209.0
46	120632.5
47	116871.0
48	111767.0
49	111075.0
50	111351.0
51	108037.5
52	103495.0
53	98951.5
54	95083.0
55	92132.5
56	84940.5
57	75105.5
58	69010.5
59	64981.5
60	58917.0
61	50090.0
62	42289.5
63	35784.5
64	29479.5
65	24496.5
66	20187.5
67	15853.5
68	12256.5
69	9369.5
70	7026.0
71	5472.0
72	4229.5
73	3123.5
74	2441.5
75	1920.0
76	1405.0
77	1034.5
78	815.0
79	631.5
80	427.5
81	283.5
82	210.0
83	165.0
84	132.0
85	111.5
86	101.0
87	89.0
88	68.5
89	52.0
90	49.5
91	47.0
92	36.0
93	23.0
94	24.5
95	23.5
96	17.0
97	15.5
98	14.0
99	16.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.753421539231837E-4
2	1.3203948720088435E-4
3	2.640789744017687E-5
4	1.848552820812381E-4
5	2.640789744017687E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.3203948720088435E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0563158976070748E-4
84-85	7.658290257651292E-4
86-87	0.0015580659489704352
88-89	0.003433026667222993
90-91	0.0029444805645797213
92-93	0.0021126317952141495
94-95	0.0022578752311351222
96-97	0.0020730199490538843
98-99	0.002733217385058306
100	0.003195355590261401
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3786746.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.40999259948496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0449730319732	39.7437644209924
2	15.089511826201948	16.420404535869775
3	5.319907217073363	8.683683369327246
4	2.325036030780616	5.060207729132368
5	1.2488503976317247	3.3974970446502994
6	0.7370580149839694	2.4061992684401305
7	0.49294672489033187	1.8774859357256313
8	0.33108075883405547	1.4411281310394257
9	0.23430581353866428	1.1473719822589523
>10	1.0555679103387865	10.067809106951426
>50	0.07085403030044339	2.6787865446540606
>100	0.04621241120897835	4.8012523733588655
>500	0.0030150210630232447	1.0712022299438952
>1k	6.321818108830118E-4	0.4451501974859671
>5k	0.0	0.0
>10k+	4.8629370067923765E-5	0.758057130169574
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28650	0.7565862616610672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.640789744017687E-5	0.0
18-19	0.0	0.0	0.0	7.922369232053061E-5	0.0
20-21	0.0	0.0	0.0	1.4524343592097277E-4	0.0
22-23	0.0	0.0	0.0	3.5650661544238773E-4	0.0
24-25	0.0	0.0	0.0	8.318487693655714E-4	0.0
26-27	0.0	0.0	0.0	0.001333598820728932	0.0
28-29	0.0	0.0	0.0	0.0027464213337783944	0.0
30-31	0.0	0.0	0.0	0.0062850795907620945	0.0
32-33	0.0	0.0	0.0	0.013613271130411176	0.0
34-35	0.0	0.0	0.0	0.025140318363048378	0.0
36-37	0.0	0.0	0.0	0.04308448467364856	0.0
38-39	0.0	0.0	0.0	0.06543876985675828	0.0
40-41	0.0	0.0	0.0	0.08944354862987905	0.0
42-43	0.0	0.0	0.0	0.11284094576187575	0.0
44-45	0.0	0.0	0.0	0.14334206730528004	0.0
46-47	0.0	0.0	0.0	0.17886068936231792	0.0
48-49	0.0	0.0	0.0	0.2171521406505744	0.0
50-51	0.0	0.0	0.0	0.2573053487083633	0.0
52-53	0.0	0.0	0.0	0.29873933979200085	0.0
54-55	0.0	0.0	0.0	0.3447947129276693	0.0
56-57	0.0	0.0	0.0	0.3991949816544337	0.0
58-59	0.0	0.0	0.0	0.4643960804342303	0.0
60-61	0.0	0.0	0.0	0.5344430283942995	2.640789744017687E-5
62-63	0.0	0.0	0.0	0.6059424107135783	2.640789744017687E-5
64-65	0.0	0.0	0.0	0.6810596749821615	2.640789744017687E-5
66-67	0.0	0.0	0.0	0.7690507892528308	2.640789744017687E-5
68-69	0.0	0.0	0.0	0.8734280038851299	2.640789744017687E-5
70-71	0.0	0.0	0.0	0.9896227526219081	2.640789744017687E-5
72-73	0.0	0.0	0.0	1.1104916992055975	2.640789744017687E-5
74-75	0.0	0.0	0.0	1.2348861000975506	2.640789744017687E-5
76-77	0.0	0.0	0.0	1.3735539695559194	2.640789744017687E-5
78-79	0.0	0.0	0.0	1.5343384531204363	2.640789744017687E-5
80-81	0.0	0.0	0.0	1.7183222745861486	2.640789744017687E-5
82-83	0.0	0.0	0.0	1.916936071233719	2.640789744017687E-5
84-85	0.0	0.0	0.0	2.1181378418304266	2.640789744017687E-5
86-87	0.0	0.0	0.0	2.332041811095859	2.640789744017687E-5
88	0.0	0.0	0.0	2.509384046355367	2.640789744017687E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4895	0.0	51.369205	1
GTATCAA	9170	0.0	39.876038	1
TCAACGC	10630	0.0	33.20494	4
ATCAACG	10750	0.0	32.965443	3
CAACGCA	11675	0.0	30.353619	5
TATCAAC	12045	0.0	29.850435	2
AACGCAG	12110	0.0	29.146862	6
ACGCAGA	13660	0.0	25.495499	7
GTACATG	21820	0.0	25.417278	1
TACATGG	21170	0.0	25.331493	2
CGCAGAG	13880	0.0	25.02367	8
GTGGTAT	2195	0.0	23.339617	1
ACATGGG	22195	0.0	23.102854	3
GCAGAGT	15395	0.0	21.88948	9
TGGTATC	2310	0.0	20.549696	2
CATGGGG	13325	0.0	19.611176	4
CAGAGTA	14950	0.0	18.847078	10-11
TAGACCG	180	1.3385688E-4	18.2777	5
AGAGTAC	14280	0.0	17.921143	10-11
CATGGGA	6040	0.0	17.741648	4
>>END_MODULE