FastQCFastQC Report
Wed 25 May 2016
SRR2431346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786746
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267590.7066489276016928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201150.5311948570091578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153400.40509714673231323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47470.1253582891485196No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44900.11857145950639415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42130.11125647191546516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38060.10050845765731317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89450.063.2201231
GTATCAA184450.047.4538151
ATCAACG245500.035.4378783
TCAACGC246650.035.2531244
TATCAAC252200.034.5896072
CAACGCA255600.033.9622045
AACGCAG262550.033.0989886
GTGGTAT29000.033.067571
TGGTATC30100.031.386462
ACGCAGA293100.029.4887077
CGCAGAG296500.029.0712998
GCAGAGT318300.026.8882859
GTACATG200450.024.8582121
TACATGG198750.024.2634722
ACATGGG202550.023.2745573
GAGTACT205250.023.12746412-13
CAGAGTA312950.022.48208810-11
AGAGTAC306350.022.36044710-11
TATAACG2807.6397555E-1121.8221782
AGTACTT212600.021.81944512-13
Produced by FastQC (version 0.11.5)