##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431346_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3786746
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73697628517994	34.0	31.0	34.0	31.0	34.0
2	32.8668548141333	34.0	31.0	34.0	31.0	34.0
3	32.939116856530646	34.0	31.0	34.0	31.0	34.0
4	36.33148882972346	37.0	37.0	37.0	35.0	37.0
5	36.284252231335294	37.0	37.0	37.0	35.0	37.0
6	36.317001985345726	37.0	37.0	37.0	35.0	37.0
7	36.185566182680326	37.0	37.0	37.0	35.0	37.0
8	36.23893522301206	37.0	37.0	37.0	35.0	37.0
9	38.05295522857884	39.0	39.0	39.0	35.0	39.0
10-11	38.04666790431679	39.0	39.0	39.0	35.0	39.0
12-13	38.02218197365231	39.0	39.0	39.0	35.0	39.0
14-15	39.4950017772515	41.0	39.0	41.0	36.0	41.0
16-17	39.50604199489483	41.0	39.0	41.0	36.5	41.0
18-19	39.579635787560086	41.0	40.0	41.0	37.0	41.0
20-21	39.5827223426129	41.0	40.0	41.0	37.0	41.0
22-23	39.50745415721044	41.0	40.0	41.0	36.0	41.0
24-25	39.468690532715954	41.0	40.0	41.0	36.0	41.0
26-27	39.375343632765436	41.0	39.0	41.0	36.0	41.0
28-29	39.28460372045022	41.0	39.0	41.0	36.0	41.0
30-31	39.10632255239723	41.0	39.0	41.0	35.0	41.0
32-33	38.898299357812746	41.0	38.5	41.0	35.0	41.0
34-35	38.789839218157226	41.0	38.0	41.0	35.0	41.0
36-37	38.67636870812038	41.0	38.0	41.0	35.0	41.0
38-39	38.5513782809832	41.0	38.0	41.0	35.0	41.0
40-41	38.44252281510299	41.0	38.0	41.0	35.0	41.0
42-43	38.29309716046442	41.0	38.0	41.0	34.5	41.0
44-45	38.06498693073156	40.0	38.0	41.0	34.0	41.0
46-47	37.73348780192809	40.0	38.0	41.0	33.0	41.0
48-49	37.43716016336982	40.0	37.5	41.0	33.0	41.0
50-51	37.266219202449804	40.0	37.0	41.0	33.0	41.0
52-53	37.13722230115249	40.0	37.0	41.0	32.5	41.0
54-55	36.977265837212215	40.0	36.5	41.0	32.0	41.0
56-57	36.7941878066287	40.0	36.0	41.0	32.0	41.0
58-59	36.59077635521369	40.0	35.0	41.0	31.5	41.0
60-61	36.38167492617673	39.0	35.0	41.0	31.0	41.0
62-63	36.14317820630167	39.0	35.0	41.0	31.0	41.0
64-65	35.86709182501282	38.5	35.0	41.0	31.0	41.0
66-67	35.57701440762069	37.5	35.0	41.0	30.0	41.0
68-69	35.30604059527626	37.0	35.0	40.0	30.0	41.0
70-71	35.05243684155209	37.0	35.0	40.0	30.0	41.0
72-73	34.79541075635915	36.0	35.0	39.0	30.0	41.0
74-75	34.50191655315672	36.0	35.0	39.0	30.0	41.0
76-77	34.19168753330696	35.5	35.0	38.5	30.0	40.5
78-79	33.88600687767281	35.0	35.0	37.0	30.0	39.5
80-81	33.590432524389016	35.0	35.0	37.0	29.0	39.0
82-83	33.28473589197691	35.0	34.0	36.5	29.0	39.0
84-85	33.03441648845737	35.0	34.0	36.0	29.0	37.0
86-87	32.82384189486171	35.0	34.0	36.0	29.0	37.0
88-89	32.61198493376635	35.0	34.0	35.5	29.0	37.0
90-91	32.42446575503084	35.0	34.0	35.0	29.0	36.0
92-93	32.28590945893915	35.0	34.0	35.0	29.0	36.0
94-95	32.15902202048936	35.0	34.0	35.0	29.0	36.0
96-97	32.0274124538588	35.0	34.0	35.0	27.5	36.0
98-99	31.910427976949073	35.0	34.0	35.0	27.0	36.0
100	31.809742454339425	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22101586153288366
1101	2	0.2088888073934072
1101	3	0.23503247667238014
1101	4	0.14523994979073507
1101	5	0.16167811341955485
1101	6	0.1700235580148899
1101	7	0.20786660356279185
1101	8	0.21081587224439602
1101	9	0.2508450781045184
1101	10-11	0.2233573828979516
1101	12-13	0.21263628378839172
1101	14-15	0.301583615153568
1101	16-17	0.30425321827775775
1101	18-19	0.2922485988829777
1101	20-21	0.31260116576072505
1101	22-23	0.33187160393548254
1101	24-25	0.3336801513513947
1101	26-27	0.30773162303156454
1101	28-29	0.2999835875477359
1101	30-31	0.3516067421771041
1101	32-33	0.3281965675286074
1101	34-35	0.32745983906836074
1101	36-37	0.31345032238897375
1101	38-39	0.36659738982844914
1101	40-41	0.36503198851513474
1101	42-43	0.4011390139882707
1101	44-45	0.3924865890516358
1101	46-47	0.37013490148204653
1101	48-49	0.3315822847221881
1101	50-51	0.3431708356309926
1101	52-53	0.3075936318595254
1101	54-55	0.2536547331383616
1101	56-57	0.2806989242190028
1101	58-59	0.2861644961437406
1101	60-61	0.23382760741466058
1101	62-63	0.2767872414557644
1101	64-65	0.312441081119168
1101	66-67	0.29376468921132215
1101	68-69	0.27867179546696974
1101	70-71	0.2581620392918822
1101	72-73	0.23632859230990277
1101	74-75	0.24912970311844873
1101	76-77	0.22747732375205487
1101	78-79	0.23944699966657623
1101	80-81	0.2581629336971858
1101	82-83	0.29971710802344376
1101	84-85	0.26667626730255023
1101	86-87	0.26839447778350944
1101	88-89	0.2535965515908032
1101	90-91	0.24122805650725354
1101	92-93	0.2860731644616621
1101	94-95	0.2949589733083897
1101	96-97	0.2585215814582469
1101	98-99	0.26229958468133674
1101	100	0.2727530779845182
1102	1	0.08770791582189474
1102	2	0.12066555399491818
1102	3	0.12769943193338662
1102	4	0.08904491788081259
1102	5	0.08949712498755957
1102	6	0.09046710364588506
1102	7	0.16112488066904973
1102	8	0.19402640832466034
1102	9	0.17401551476077515
1102	10-11	0.18419091784951291
1102	12-13	0.18030143985536284
1102	14-15	0.24622427722803764
1102	16-17	0.24034581004659827
1102	18-19	0.23299088666939127
1102	20-21	0.21546351168552036
1102	22-23	0.21409195041503892
1102	24-25	0.21710344438640305
1102	26-27	0.17971518834338696
1102	28-29	0.18489115237206732
1102	30-31	0.21526551806696403
1102	32-33	0.23918893786095907
1102	34-35	0.23758850585292635
1102	36-37	0.16279637412547743
1102	38-39	0.20378204716968895
1102	40-41	0.23851704001548057
1102	42-43	0.2616828334945751
1102	44-45	0.30078546646305426
1102	46-47	0.2612723790734961
1102	48-49	0.23221062837511397
1102	50-51	0.22909462864303265
1102	52-53	0.17788463476825456
1102	54-55	0.21825059076081033
1102	56-57	0.17559127455227497
1102	58-59	0.21294838346608458
1102	60-61	0.19667519090721441
1102	62-63	0.17970730624845288
1102	64-65	0.3092362373633435
1102	66-67	0.2925853582077167
1102	68-69	0.27611252784563334
1102	70-71	0.27465093835510146
1102	72-73	0.24389388677919754
1102	74-75	0.22482535832705253
1102	76-77	0.2183307637568035
1102	78-79	0.169906034640384
1102	80-81	0.1804238822901425
1102	82-83	0.22963407024615634
1102	84-85	0.20865942033675822
1102	86-87	0.20114734953225621
1102	88-89	0.2328466909153235
1102	90-91	0.24569468679222695
1102	92-93	0.21929189184741205
1102	94-95	0.2355313412321749
1102	96-97	0.2056356561463204
1102	98-99	0.20284361755938818
1102	100	0.2554039291027479
1103	1	0.12399951384484353
1103	2	0.13970622058032944
1103	3	0.15459078379821278
1103	4	0.06840198402177577
1103	5	0.07306007585813745
1103	6	0.13036359798594077
1103	7	0.10243406329984595
1103	8	0.11849386512128035
1103	9	0.21160533761950262
1103	10-11	0.15920931694589768
1103	12-13	0.15988300742343142
1103	14-15	0.24734134611914982
1103	16-17	0.24266025554288007
1103	18-19	0.2287476652716549
1103	20-21	0.24746087579520548
1103	22-23	0.2874296181777538
1103	24-25	0.26087482159023523
1103	26-27	0.24797549716863188
1103	28-29	0.2857566427280389
1103	30-31	0.27184487018340775
1103	32-33	0.30981055023588056
1103	34-35	0.3099731614023753
1103	36-37	0.24569982463984275
1103	38-39	0.24293020341939808
1103	40-41	0.2185258752904531
1103	42-43	0.20012163940873506
1103	44-45	0.23166760890168803
1103	46-47	0.28348206222960215
1103	48-49	0.2255491712494191
1103	50-51	0.22744507538856595
1103	52-53	0.19563162324680405
1103	54-55	0.26373658401853106
1103	56-57	0.2080033538309678
1103	58-59	0.16715007450690678
1103	60-61	0.04778788067430639
1103	62-63	0.16023309568718958
1103	64-65	0.13907160868917146
1103	66-67	0.1364727251410187
1103	68-69	0.09864496100033904
1103	70-71	0.10566300312705579
1103	72-73	0.13002589283379962
1103	74-75	0.10704143614537287
1103	76-77	0.07531260301598763
1103	78-79	0.03323018631526509
1103	80-81	0.013950145515856605
1103	82-83	0.07680492433723884
1103	84-85	0.1120134548971663
1103	86-87	0.07631298419249077
1103	88-89	0.13246761140291596
1103	90-91	0.18366567887564145
1103	92-93	0.2018297782239813
1103	94-95	0.17811259163470083
1103	96-97	0.18729201202047108
1103	98-99	0.19414781126958758
1103	100	0.21513824037388218
1104	1	0.0824739064849851
1104	2	0.11483175029979265
1104	3	0.10740788759634512
1104	4	0.06054886244944413
1104	5	0.05387901432474962
1104	6	0.0878271900436971
1104	7	0.10228004913784616
1104	8	0.13209527125039955
1104	9	0.13055999555095354
1104	10-11	0.09982831468673936
1104	12-13	0.1522886929748779
1104	14-15	0.1891764154639759
1104	16-17	0.20727828563214956
1104	18-19	0.21058206719857964
1104	20-21	0.1937838433537209
1104	22-23	0.20619127124035685
1104	24-25	0.22638544482666845
1104	26-27	0.18614081198926868
1104	28-29	0.13888620110443384
1104	30-31	0.15463816163830302
1104	32-33	0.22853453490029807
1104	34-35	0.22432488026618813
1104	36-37	0.25648446972715533
1104	38-39	0.191279365425423
1104	40-41	0.2534198785058166
1104	42-43	0.2564552421566546
1104	44-45	0.20673192964895293
1104	46-47	0.1800267374544191
1104	48-49	0.21753126565899095
1104	50-51	0.21032479711357865
1104	52-53	0.20292488628945904
1104	54-55	0.1881177357333712
1104	56-57	0.17788972262766123
1104	58-59	0.17603955549623151
1104	60-61	0.2247181216194747
1104	62-63	0.24039861532648388
1104	64-65	0.2025217722848467
1104	66-67	0.18177148808297972
1104	68-69	0.18266488860950147
1104	70-71	0.16190754142680674
1104	72-73	0.13642080372707
1104	74-75	0.12054112889999402
1104	76-77	0.11180992288770852
1104	78-79	0.11629926960077341
1104	80-81	0.15985961671523086
1104	82-83	0.15460234574486265
1104	84-85	0.05683193097331696
1104	86-87	0.061489190695631635
1104	88-89	0.07913893769973157
1104	90-91	0.1153235473760077
1104	92-93	0.1105992840601786
1104	94-95	0.09985293635430281
1104	96-97	0.117452070176995
1104	98-99	0.10304443272570296
1104	100	0.08166621310035893
1105	1	-0.05403120448001175
1105	2	0.005532968014435369
1105	3	0.030907868223870594
1105	4	-0.06317223816559192
1105	5	-0.0803655530990568
1105	6	-0.11898204636061394
1105	7	-0.4307457437720501
1105	8	-0.3772119057753045
1105	9	-0.3399870649703516
1105	10-11	-0.3224735554031142
1105	12-13	-0.18568748393121837
1105	14-15	-0.18506124946178915
1105	16-17	-0.17515411284342974
1105	18-19	-0.16081265152708824
1105	20-21	-0.1152259521009924
1105	22-23	-0.09937052148873704
1105	24-25	-0.17504222957195026
1105	26-27	-0.15322477867196227
1105	28-29	-0.17367582942883786
1105	30-31	-0.17114909444395465
1105	32-33	-0.13656209305322875
1105	34-35	-0.22809540198800704
1105	36-37	-0.20876953504128437
1105	38-39	-0.24946485778595928
1105	40-41	-0.30284781642762937
1105	42-43	-0.25075024915464894
1105	44-45	-0.28160476836556114
1105	46-47	-0.22177741928970107
1105	48-49	-0.2479545595832917
1105	50-51	-0.22724307674857158
1105	52-53	-0.24184925097549836
1105	54-55	-0.21329514609394806
1105	56-57	-0.19308599607722243
1105	58-59	-0.1967345924376218
1105	60-61	-0.21740247752914144
1105	62-63	-0.23450386600715234
1105	64-65	-0.19827971835712077
1105	66-67	-0.1529817683182273
1105	68-69	-0.1804888763613448
1105	70-71	-0.2570224471406277
1105	72-73	-0.21199831435351513
1105	74-75	-0.27519809115844396
1105	76-77	-0.2822982363226174
1105	78-79	-0.32472896045104704
1105	80-81	-0.28679888005350307
1105	82-83	-0.29042419525150365
1105	84-85	-0.2768910570544918
1105	86-87	-0.25047427589976223
1105	88-89	-0.24066362657056573
1105	90-91	-0.29222805851816247
1105	92-93	-0.2736214362646905
1105	94-95	-0.3299339397185541
1105	96-97	-0.3165101890076656
1105	98-99	-0.31762041035441513
1105	100	-0.2826190519684779
1106	1	0.12051184583064156
1106	2	0.091310741941939
1106	3	0.07016705906603704
1106	4	0.05379891615655907
1106	5	0.08873120249496935
1106	6	0.0737777635356025
1106	7	0.08042921090621036
1106	8	0.11037558511215195
1106	9	0.12012651555383513
1106	10-11	0.11694483472286521
1106	12-13	0.1048799666447735
1106	14-15	0.12908562058949258
1106	16-17	0.16453736713866363
1106	18-19	0.1118314452871303
1106	20-21	0.09827952871588508
1106	22-23	0.1044201130520861
1106	24-25	0.11232993420431825
1106	26-27	0.148859982228835
1106	28-29	0.12366977117083877
1106	30-31	0.13026230513927572
1106	32-33	0.1219664120927888
1106	34-35	0.13011484137631157
1106	36-37	0.15836796074440684
1106	38-39	0.11131324833595357
1106	40-41	0.1536624481189648
1106	42-43	0.20579115047548413
1106	44-45	0.1539666965518478
1106	46-47	0.1880305782059395
1106	48-49	0.2097173594894599
1106	50-51	0.2558969824054813
1106	52-53	0.24292668650316784
1106	54-55	0.267118850858715
1106	56-57	0.23925349409945795
1106	58-59	0.25070028378023324
1106	60-61	0.24386236830398644
1106	62-63	0.24917407125577995
1106	64-65	0.30701000531966116
1106	66-67	0.27087373727645314
1106	68-69	0.2761976303448037
1106	70-71	0.2509449588561736
1106	72-73	0.27046128364175104
1106	74-75	0.2630086296424494
1106	76-77	0.1870136607445403
1106	78-79	0.16462474621321377
1106	80-81	0.17381209645736106
1106	82-83	0.21601212021488436
1106	84-85	0.19580801793389924
1106	86-87	0.18895394978608238
1106	88-89	0.2537634051694937
1106	90-91	0.2385614939807965
1106	92-93	0.2001561632281792
1106	94-95	0.2770117177059035
1106	96-97	0.29097126216861824
1106	98-99	0.2523005032766079
1106	100	0.19582231272261907
1107	1	-0.05088662816578449
1107	2	-0.008526697696183305
1107	3	0.031126562089845322
1107	4	-0.030582210813598465
1107	5	-0.04890554142499326
1107	6	-0.06938894418195929
1107	7	-0.013110761166295504
1107	8	-0.017971853003153626
1107	9	-0.008472383075478263
1107	10-11	0.021256434984486816
1107	12-13	0.021185618320146204
1107	14-15	0.01992896935930588
1107	16-17	0.046080762940526654
1107	18-19	0.005601533752901844
1107	20-21	0.011222302424648944
1107	22-23	0.024506781839981784
1107	24-25	0.007586070829425751
1107	26-27	0.0515692063833697
1107	28-29	0.05169311666536913
1107	30-31	0.0858532799651357
1107	32-33	0.058573172575748345
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2216	38-39	-0.6936483338843189
2216	40-41	-0.6849586542746238
2216	42-43	-0.714668180273307
2216	44-45	-0.6626798554866582
2216	46-47	-0.6773321607164249
2216	48-49	-0.6878168559318567
2216	50-51	-0.6874403925154979
2216	52-53	-0.7152013331183795
2216	54-55	-0.7766445880002522
2216	56-57	-0.7395781925126172
2216	58-59	-0.8160232364693485
2216	60-61	-0.7413658381546142
2216	62-63	-0.8505553698465604
2216	64-65	-0.7265094866780331
2216	66-67	-0.6869063492114051
2216	68-69	-0.7112163900856601
2216	70-71	-0.6703025520972901
2216	72-73	-0.7621791012557964
2216	74-75	-0.6729702993262308
2216	76-77	-0.599437848500699
2216	78-79	-0.6291765462777406
2216	80-81	-0.6227592320164845
2216	82-83	-0.6368589201527186
2216	84-85	-0.6017785116607541
2216	86-87	-0.5476044549887362
2216	88-89	-0.5240148055334544
2216	90-91	-0.5089144934252658
2216	92-93	-0.6063531432927718
2216	94-95	-0.6240711054860633
2216	96-97	-0.7245251346245993
2216	98-99	-0.6637958622350411
2216	100	-0.6973116890841702
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46.0
3	453.0
4	297.0
5	450.0
6	613.0
7	908.0
8	1619.0
9	2513.0
10	3049.0
11	4033.0
12	6050.0
13	9039.0
14	9765.0
15	12896.0
16	17172.0
17	26861.0
18	32964.0
19	17949.0
20	11059.0
21	9943.0
22	9763.0
23	10048.0
24	10927.0
25	12886.0
26	16334.0
27	20462.0
28	25398.0
29	31756.0
30	39929.0
31	49632.0
32	62285.0
33	80617.0
34	112106.0
35	192278.0
36	335075.0
37	736815.0
38	1214007.0
39	653398.0
40	5351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.0962552467508	21.2994114976504	14.923973778724914	23.68035947687389
2	13.738534672144374	22.132819681715922	40.01166701833211	24.11697862780759
3	15.486796124042677	28.722150436432077	31.698043350870897	24.093010088654353
4	10.619610183658136	18.79970580895276	39.37483082034143	31.205853187047673
5	11.851389028997456	38.89339290250785	35.28335409874335	13.971863969751338
6	27.8579022728221	39.758304359468525	18.518009921975224	13.865783445734149
7	24.745097769958694	34.48108217451078	23.572956834179003	17.200863221351526
8	25.259787691067743	34.657645376795806	21.035844495511448	19.046722436625007
9	26.081469419918847	15.774704720094773	21.82126818117719	36.32255767880919
10-11	23.443848623593976	27.216771338769487	29.066235232043553	20.27314480559298
12-13	24.808978473866482	25.762475222790226	29.135067416721377	20.29347888662192
14-15	22.187426883133963	26.4931685410112	27.968524955199005	23.350879620655835
16-17	20.315661520471668	29.772461633286202	28.120568424710818	21.791308421531312
18-19	20.27401626620851	27.647933080275255	31.91533047106936	20.16272018244688
20-21	21.837139327538736	26.561498975637658	31.69671269211085	19.904649004712756
22-23	21.386481916845415	26.689139808159652	31.6128968367931	20.31148143820183
24-25	20.952842965383297	26.94261015704399	31.55966680059888	20.544880076973833
26-27	20.71438314119483	26.97692449788029	31.660751919569474	20.647940441355406
28-29	20.742843025809883	26.808163342913772	31.959999033461383	20.488994597814962
30-31	20.912241422838555	26.967499796655858	31.700704362972985	20.419554417532595
32-33	20.579530027778326	26.890373926589007	31.86514605308661	20.664949992546056
34-35	21.017815152148973	26.780749346635147	31.58740458337823	20.614030917837653
36-37	21.23419426398352	26.871850736808746	31.192006021232764	20.701948977974965
38-39	21.05019086816958	26.88251737355892	31.241706048494116	20.82558570977739
40-41	20.874118440876614	26.935323501332988	31.32787463209225	20.86268342569815
42-43	21.026919175134076	27.005054104147263	31.123323072486524	20.844703648232134
44-45	21.052992257421522	27.035896208938443	30.82579581342496	21.08531572021508
46-47	21.36000941210398	27.15219020900866	30.33936524020738	21.148435138679982
48-49	21.442427406643617	27.205080679971537	29.87191741742753	21.48057449595732
50-51	21.500990040441394	27.486733880615226	29.43343489896517	21.57884117997821
52-53	21.398404170336267	27.547022574797086	29.494579117421427	21.55999413744522
54-55	21.52430237283946	27.73261566909066	29.385744266357587	21.357337691712296
56-57	21.495801707995987	27.54585533622317	29.387053724748753	21.571289231032093
58-59	21.519644367825837	27.14369144918632	29.61531498949685	21.72134919349099
60-61	21.619368375797638	27.35719570146217	29.5131722506189	21.510263672121287
62-63	21.292067120424765	27.252646467441966	29.837002534629995	21.61828387750327
64-65	21.44172067521825	27.25100917780068	29.784622470057403	21.522647676923672
66-67	21.496636527054743	27.18621780220355	29.603830342479263	21.713315328262443
68-69	21.68750136992234	27.300472626508864	29.409624869708832	21.602401133859964
70-71	21.67566734628961	27.241154701433995	29.46327216990917	21.619905782367223
72-73	21.556910105357	27.282224517949988	29.388664773747582	21.772200602945432
74-75	21.618633548081952	27.272009444696277	29.314949221289226	21.794407785932542
76-77	21.593042438473944	27.347111451598465	29.189743971644127	21.87010213828346
78-79	21.634665471257023	27.46801806054468	29.165125640150446	21.732190828047848
80-81	21.417268749968642	27.493752497218566	29.289346723662447	21.79963202915035
82-83	21.405229383855094	27.467423139180475	29.37199779702556	21.75534967993887
84-85	21.50265303387314	27.250622408282666	29.124482316738643	22.122242241105553
86-87	21.52833408706043	27.39919257672337	29.092814064877746	21.979659271338452
88-89	21.277038505731795	27.35254046879551	29.22452183084774	22.145899194624953
90-91	21.313456256527	27.282798477344294	29.144538075437453	22.25920719069125
92-93	21.30384221437972	27.33993260236704	29.26297422920609	22.093250954047154
94-95	21.26475559194021	27.187868064541686	29.279055642115825	22.268320701402278
96-97	21.273625066549485	27.39081659384956	28.984676717399203	22.35088162220175
98-99	20.95581744831104	27.29073304487366	29.272714812732946	22.48073469408235
100	21.16121218651396	27.281749490045453	29.067435480582642	22.48960284285794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3967.0
1	2433.0
2	2361.0
3	3342.5
4	1809.0
5	1140.5
6	2892.5
7	8029.0
8	13866.0
9	14269.5
10	8743.5
11	4157.5
12	3413.0
13	3576.0
14	3889.0
15	4280.5
16	4808.0
17	5368.0
18	6036.5
19	6811.5
20	7917.5
21	9388.0
22	11538.0
23	14713.0
24	19198.5
25	24773.0
26	31537.0
27	40182.0
28	50068.5
29	61382.5
30	71037.0
31	78688.0
32	86213.5
33	92715.0
34	98555.5
35	105414.0
36	110857.0
37	115379.0
38	122758.0
39	126859.5
40	128535.5
41	129900.0
42	127904.5
43	127026.5
44	126456.0
45	122818.0
46	119812.0
47	116153.0
48	109920.5
49	108615.5
50	110381.0
51	107234.0
52	101520.5
53	96850.0
54	92616.0
55	88535.0
56	81225.5
57	72397.0
58	67175.0
59	63871.0
60	57961.5
61	48390.0
62	40622.5
63	34208.5
64	27530.0
65	22722.5
66	18612.5
67	14202.0
68	10740.0
69	8091.5
70	5932.5
71	4395.5
72	3277.5
73	2411.0
74	1854.0
75	1410.0
76	966.0
77	712.0
78	552.0
79	366.0
80	200.5
81	96.0
82	53.0
83	39.0
84	18.5
85	5.0
86	3.0
87	3.0
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	1.0
94	2.5
95	6.0
96	6.0
97	5.5
98	4.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00937480359126279
2	7.922369232053061E-5
3	4.4893425648300677E-4
4	0.002403118667056095
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.242764104061905E-5
24-25	1.1883553848079592E-4
26-27	6.469934872843333E-4
28-29	9.902961540066326E-4
30-31	0.001637289641290966
32-33	0.0042384675391483875
34-35	0.005123132103394313
36-37	0.003855553026265823
38-39	0.002667197641457864
40-41	0.00345943456466317
42-43	5.677697949638027E-4
44-45	6.073816411240679E-4
46-47	0.0034990464108234347
48-49	0.002310691026015476
50-51	8.450527180856598E-4
52-53	2.640789744017687E-5
54-55	4.4893425648300677E-4
56-57	7.26217179604864E-4
58-59	6.469934872843333E-4
60-61	4.3573030776291834E-4
62-63	0.0
64-65	0.0
66-67	9.242764104061905E-4
68-69	9.242764104061905E-4
70-71	0.002310691026015476
72-73	5.281579488035374E-5
74-75	0.0018617567695324694
76-77	4.4893425648300677E-4
78-79	0.0010167040514468095
80-81	8.714606155258367E-4
82-83	0.0012543751284084014
84-85	0.0011487435386476938
86-87	2.2446712824150339E-4
88-89	0.0016636975387311428
90-91	0.0019145725644128231
92-93	0.0030237042569002514
94-95	0.0012147632822481359
96-97	0.002376710769615918
98-99	0.002152243641374415
100	0.0035386582569837005
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3786746.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.38999953640241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.41079744059164	35.48000050389137
2	16.307101703002708	16.434296945087468
3	5.819312256932222	8.797054257869865
4	2.6655377413883334	5.372657822112849
5	1.4508194169604833	3.6553394874021183
6	0.828274215610903	2.5042042404388476
7	0.5328431065191245	1.879497472333173
8	0.3558836611094473	1.434638201465459
9	0.2509014745118043	1.1378632665899249
>10	1.2360175945055045	10.972534827256215
>50	0.0800585817624541	2.7928360229886007
>100	0.05741807576958326	5.558284425088494
>500	0.00382849363707207	1.2890079747836793
>1k	0.0010489023467886143	1.0476493026730314
>5k	0.0	0.0
>10k+	1.5733535201829183E-4	1.6441352500189164
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26759	0.7066489276016928	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20115	0.5311948570091578	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15340	0.40509714673231323	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4747	0.1253582891485196	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4490	0.11857145950639415	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4213	0.11125647191546516	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3806	0.10050845765731317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.640789744017687E-5	0.0
18-19	0.0	0.0	0.0	5.281579488035374E-5	0.0
20-21	0.0	0.0	0.0	1.4524343592097277E-4	0.0
22-23	0.0	0.0	0.0	3.5650661544238773E-4	0.0
24-25	0.0	0.0	0.0	8.318487693655714E-4	0.0
26-27	0.0	0.0	0.0	0.001307190923288755	0.0
28-29	0.0	0.0	0.0	0.002733217385058306	0.0
30-31	0.0	0.0	0.0	0.006271875642042006	0.0
32-33	0.0	0.0	0.0	0.013679290874011618	0.0
34-35	0.0	0.0	0.0	0.025325173645129616	0.0
36-37	0.0	0.0	0.0	0.043454195237811036	0.0
38-39	0.0	0.0	0.0	0.0658745001645212	0.0
40-41	0.0	0.0	0.0	0.09003772632228303	0.0
42-43	0.0	0.0	0.0	0.11361997873636098	0.0
44-45	0.0	0.0	0.0	0.14393624499768404	0.0
46-47	0.0	0.0	0.0	0.17956049864448262	0.0
48-49	0.0	0.0	0.0	0.21812923285586094	0.0
50-51	0.0	0.0	0.0	0.2587049672726927	0.0
52-53	0.0	0.0	0.0	0.3004690570743324	0.0
54-55	0.0	0.0	0.0	0.3466696736459218	0.0
56-57	0.0	0.0	0.0	0.40133402134708795	0.0
58-59	0.0	0.0	0.0	0.46678599515256636	0.0
60-61	0.0	0.0	0.0	0.5375987721384006	0.0
62-63	0.0	0.0	0.0	0.6096659242526433	0.0
64-65	0.0	0.0	0.0	0.6853905701623504	0.0
66-67	0.0	0.0	0.0	0.774147513458785	0.0
68-69	0.0	0.0	0.0	0.8795018202963706	0.0
70-71	0.0	0.0	0.0	0.9972942468282795	0.0
72-73	0.0	0.0	0.0	1.119325140899337	0.0
74-75	0.0	0.0	0.0	1.245079548509459	0.0
76-77	0.0	0.0	0.0	1.3850678128398366	0.0
78-79	0.0	0.0	0.0	1.547872500558527	0.0
80-81	0.0	0.0	0.0	1.733889730127133	0.0
82-83	0.0	0.0	0.0	1.934853829646879	0.0
84-85	0.0	0.0	0.0	2.1382078438849605	0.0
86-87	0.0	0.0	0.0	2.355940958279219	0.0
88	2.640789744017687E-5	0.0	0.0	2.535844759590424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8945	0.0	63.220123	1
GTATCAA	18445	0.0	47.453815	1
ATCAACG	24550	0.0	35.437878	3
TCAACGC	24665	0.0	35.253124	4
TATCAAC	25220	0.0	34.589607	2
CAACGCA	25560	0.0	33.962204	5
AACGCAG	26255	0.0	33.098988	6
GTGGTAT	2900	0.0	33.06757	1
TGGTATC	3010	0.0	31.38646	2
ACGCAGA	29310	0.0	29.488707	7
CGCAGAG	29650	0.0	29.071299	8
GCAGAGT	31830	0.0	26.888285	9
GTACATG	20045	0.0	24.858212	1
TACATGG	19875	0.0	24.263472	2
ACATGGG	20255	0.0	23.274557	3
GAGTACT	20525	0.0	23.127464	12-13
CAGAGTA	31295	0.0	22.482088	10-11
AGAGTAC	30635	0.0	22.360447	10-11
TATAACG	280	7.6397555E-11	21.822178	2
AGTACTT	21260	0.0	21.819445	12-13
>>END_MODULE