FastQCFastQC Report
Wed 25 May 2016
SRR2431345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984402
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA675142.2622287480037877No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61440.2058703887747026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.11379164067039226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.047.000121
GTATCAA83300.038.988091
ATCAACG105000.029.9452883
TCAACGC105400.029.6532764
TACATGG191300.028.4256292
GTACATG201400.028.2607461
CAACGCA116550.026.8970855
AACGCAG119000.026.4618056
TATCAAC125250.025.7792722
ACGCAGA123200.025.3689567
ACATGGG210400.024.9516413
CGCAGAG124800.024.8930748
CATGGGG123050.022.3442574
GCAGAGT133500.022.179469
CATGGGA58850.020.205324
CATGGGT30400.019.7891924
CAGAGTA132400.018.76068510-11
GAGTACA138450.018.5013081
TAGCGTG2352.7996011E-617.9997439
GTATAGA8400.017.9048061
Produced by FastQC (version 0.11.5)