##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431345_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2984402
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.371046527914135	33.0	31.0	34.0	26.0	34.0
2	30.54326863472146	33.0	31.0	34.0	26.0	34.0
3	30.68897085580294	34.0	31.0	34.0	26.0	34.0
4	33.7219637300873	37.0	35.0	37.0	27.0	37.0
5	34.046026641183055	37.0	35.0	37.0	30.0	37.0
6	33.96838127035165	37.0	35.0	37.0	30.0	37.0
7	33.956479723576116	37.0	35.0	37.0	30.0	37.0
8	33.87803117676506	37.0	35.0	37.0	28.0	37.0
9	35.2460322034364	39.0	37.0	39.0	27.0	39.0
10-11	35.319085196967436	39.0	37.0	39.0	27.5	39.0
12-13	35.26225538650624	39.0	37.0	39.0	27.0	39.0
14-15	36.55880524808655	41.0	38.0	41.0	27.0	41.0
16-17	36.562095019370716	40.0	38.0	41.0	27.0	41.0
18-19	36.52371882206218	40.0	38.0	41.0	27.0	41.0
20-21	36.52574251055991	40.0	38.0	41.0	27.0	41.0
22-23	36.52977196101598	40.0	37.5	41.0	27.5	41.0
24-25	36.53981651935631	40.0	37.0	41.0	27.0	41.0
26-27	36.52807798681277	40.0	37.0	41.0	27.5	41.0
28-29	36.495535789079355	40.0	37.0	41.0	28.0	41.0
30-31	36.404391734089444	40.0	37.0	41.0	28.0	41.0
32-33	36.30318820989933	40.0	36.5	41.0	27.0	41.0
34-35	36.212690515553874	40.0	36.0	41.0	27.0	41.0
36-37	36.088442843826	40.0	36.0	41.0	27.0	41.0
38-39	35.95265567440311	40.0	36.0	41.0	26.0	41.0
40-41	35.83249005998522	40.0	35.0	41.0	26.0	41.0
42-43	35.66832015257998	40.0	35.0	41.0	25.0	41.0
44-45	35.498868114952344	40.0	35.0	41.0	25.0	41.0
46-47	35.32391866109191	40.0	35.0	41.0	25.0	41.0
48-49	35.13347816413472	39.0	35.0	41.0	25.0	41.0
50-51	34.94509888413156	39.0	35.0	41.0	25.0	41.0
52-53	34.723814687163454	39.0	34.5	41.0	25.0	41.0
54-55	34.5111193130148	38.5	34.0	41.0	25.0	41.0
56-57	34.26081389169421	38.0	34.0	40.5	24.0	41.0
58-59	33.98268463832956	37.5	33.5	40.0	23.5	41.0
60-61	33.740590409737024	37.0	33.0	40.0	23.0	41.0
62-63	33.5249312927682	36.5	33.0	40.0	22.5	41.0
64-65	33.23318373329062	36.0	33.0	40.0	22.0	41.0
66-67	32.88853679899692	35.5	33.0	39.0	20.5	41.0
68-69	32.55015544152564	35.0	33.0	39.0	19.5	41.0
70-71	32.28708230325539	35.0	32.5	39.0	18.0	41.0
72-73	32.04145570871484	35.0	32.5	37.5	17.5	40.0
74-75	31.724684878243615	35.0	32.0	37.0	14.0	39.5
76-77	31.418666955725133	35.0	32.0	36.5	9.5	39.0
78-79	31.134818466144978	35.0	32.0	36.0	7.5	39.0
80-81	30.857979085927433	35.0	31.5	36.0	4.5	38.0
82-83	30.59499842849589	35.0	31.0	35.0	2.0	37.0
84-85	30.374867226332107	35.0	31.0	35.0	2.0	37.0
86-87	30.17309832924653	35.0	31.0	35.0	2.0	36.0
88-89	29.973493014680997	35.0	31.0	35.0	2.0	36.0
90-91	29.795547650752145	35.0	31.0	35.0	2.0	36.0
92-93	29.628926163432403	35.0	30.5	35.0	2.0	36.0
94-95	29.42558760515507	35.0	30.0	35.0	2.0	35.0
96-97	29.255689917109024	34.0	30.0	35.0	2.0	35.0
98-99	29.13394308139453	34.5	30.0	35.0	2.0	35.0
100	29.09095557501972	35.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4546018959516154
1101	2	0.49313448562437756
1101	3	0.5134894254922635
1101	4	0.5663621130466154
1101	5	0.48684030029574643
1101	6	0.5014792991054975
1101	7	0.5444794949514744
1101	8	0.6064938791369769
1101	9	0.7502683362371272
1101	10-11	0.5723563579681823
1101	12-13	0.6005402744069812
1101	14-15	0.6752694960627252
1101	16-17	0.6837523999464068
1101	18-19	0.7117502207219886
1101	20-21	0.6075787849203067
1101	22-23	0.6434316897102335
1101	24-25	0.6348529995402359
1101	26-27	0.6375044872755709
1101	28-29	0.6579008743179173
1101	30-31	0.7171654946718604
1101	32-33	0.6692380310588604
1101	34-35	0.6795422135359388
1101	36-37	0.6978867671587778
1101	38-39	0.6087472282428124
1101	40-41	0.6359351076655315
1101	42-43	0.6863270706843281
1101	44-45	0.6353848058831204
1101	46-47	0.6011526348645546
1101	48-49	0.6083622723875735
1101	50-51	0.5445003207730394
1101	52-53	0.6110408791220294
1101	54-55	0.657439697529604
1101	56-57	0.5735879780553859
1101	58-59	0.633319364500565
1101	60-61	0.608036141066421
1101	62-63	0.6041013330919114
1101	64-65	0.5838915395781896
1101	66-67	0.5116903728951598
1101	68-69	0.6088611967380331
1101	70-71	0.5482885783612446
1101	72-73	0.5771846855371052
1101	74-75	0.5140271542587378
1101	76-77	0.4725910934268782
1101	78-79	0.42153967112690793
1101	80-81	0.40237589500415183
1101	82-83	0.47161256526226225
1101	84-85	0.4727713391885935
1101	86-87	0.5043374155287985
1101	88-89	0.49009936182417846
1101	90-91	0.41990075579347774
1101	92-93	0.50860864082739
1101	94-95	0.5363321026736152
1101	96-97	0.5444305111942995
1101	98-99	0.45313290595570166
1101	100	0.46251913691702384
1102	1	0.27827008405442655
1102	2	0.3293968611747893
1102	3	0.32501458193921096
1102	4	0.3743120333655625
1102	5	0.3327039651800021
1102	6	0.38640750286688785
1102	7	0.36477373181798356
1102	8	0.38645756456617164
1102	9	0.4879101379728965
1102	10-11	0.3373409770261162
1102	12-13	0.30352949386581685
1102	14-15	0.40526906572951304
1102	16-17	0.4455452652993088
1102	18-19	0.3040015158861564
1102	20-21	0.37591757376829094
1102	22-23	0.340578788709081
1102	24-25	0.42626250740561744
1102	26-27	0.4243774501336546
1102	28-29	0.43374411919695177
1102	30-31	0.4385747110211895
1102	32-33	0.42879455185644844
1102	34-35	0.501292989523904
1102	36-37	0.48464104133934427
1102	38-39	0.4722601076995403
1102	40-41	0.5614284638759344
1102	42-43	0.536747756107971
1102	44-45	0.517303106427832
1102	46-47	0.4854769839508606
1102	48-49	0.44748601678629285
1102	50-51	0.40897763249531494
1102	52-53	0.39368581812576764
1102	54-55	0.35854586212234807
1102	56-57	0.3056757049520371
1102	58-59	0.2982011894465657
1102	60-61	0.288935037747585
1102	62-63	0.31072339780292424
1102	64-65	0.29130139171013525
1102	66-67	0.27145060788485864
1102	68-69	0.25248664303521906
1102	70-71	0.24784519633085011
1102	72-73	0.26670031079738976
1102	74-75	0.33648763301138374
1102	76-77	0.3423972491353169
1102	78-79	0.26418659521612753
1102	80-81	0.29738708366778255
1102	82-83	0.2528745382428923
1102	84-85	0.3237191828027264
1102	86-87	0.2561121930048813
1102	88-89	0.31463585130461524
1102	90-91	0.2854889518921624
1102	92-93	0.22996545369629473
1102	94-95	0.12337567182955311
1102	96-97	0.18379808794631103
1102	98-99	0.20251794590728167
1102	100	0.23913316411408303
1103	1	0.12516343837166488
1103	2	0.10749748293535433
1103	3	0.17522866639712475
1103	4	0.1295552552483059
1103	5	0.12985658910871933
1103	6	0.15282894426640325
1103	7	0.24208215617564832
1103	8	0.2233942360473833
1103	9	0.2590351725674225
1103	10-11	0.23087010274732478
1103	12-13	0.21402175501902576
1103	14-15	0.22237928813041918
1103	16-17	0.19184657706860975
1103	18-19	0.1208434155810778
1103	20-21	0.12261566121315326
1103	22-23	0.10704647088282115
1103	24-25	0.14637140385519842
1103	26-27	0.14897778297542885
1103	28-29	0.18112876166053127
1103	30-31	0.15402309966216166
1103	32-33	0.10304464812445957
1103	34-35	0.14948543421741078
1103	36-37	0.12234773071561733
1103	38-39	0.09494525782485397
1103	40-41	0.05430681093922374
1103	42-43	0.0159408419762741
1103	44-45	0.007407924968944712
1103	46-47	-0.0330028029789915
1103	48-49	-0.02257451617361994
1103	50-51	-0.07981804432504447
1103	52-53	-0.05760182106126166
1103	54-55	-0.0804603192250184
1103	56-57	-0.13854667668420007
1103	58-59	-0.08060185606491643
1103	60-61	-0.07565269878602265
1103	62-63	-0.09502023416548866
1103	64-65	-0.10192114505643701
1103	66-67	0.009974634499350543
1103	68-69	0.006872746844955202
1103	70-71	-0.06730650553618034
1103	72-73	-0.12760779412029066
1103	74-75	-0.052092754566622546
1103	76-77	-0.05464898659027995
1103	78-79	-0.021831468289093436
1103	80-81	-0.04241817956499361
1103	82-83	-0.015070324395615842
1103	84-85	-0.0023084524280179153
1103	86-87	0.03577685445127088
1103	88-89	0.031578459504579826
1103	90-91	-0.030538915116157028
1103	92-93	-0.01048651487558061
1103	94-95	-0.013749909851846098
1103	96-97	-0.03782573888049967
1103	98-99	-0.017464792400225804
1103	100	-0.06264594680548896
1104	1	0.22547754944188014
1104	2	0.20279675568765398
1104	3	0.1805683994304239
1104	4	0.1838571150779842
1104	5	0.16477720186358624
1104	6	0.10259128846461607
1104	7	0.0751206294065554
1104	8	0.17630051708292171
1104	9	0.33085546849591196
1104	10-11	0.32853158118397374
1104	12-13	0.2564261953539315
1104	14-15	0.35736558913288263
1104	16-17	0.394149699903636
1104	18-19	0.26937158655184135
1104	20-21	0.240420584296281
1104	22-23	0.2788577841346296
1104	24-25	0.2559239291288833
1104	26-27	0.2759623809820866
1104	28-29	0.38832289930306985
1104	30-31	0.4435191698380905
1104	32-33	0.43756601110184334
1104	34-35	0.42199305175996926
1104	36-37	0.3968182376677447
1104	38-39	0.36654033848774503
1104	40-41	0.38802161710569294
1104	42-43	0.39422790039404276
1104	44-45	0.41837097984594607
1104	46-47	0.4854781538018642
1104	48-49	0.47141013824491296
1104	50-51	0.42835713349704463
1104	52-53	0.4085343190786901
1104	54-55	0.3316754709174887
1104	56-57	0.28078495232338696
1104	58-59	0.3156204379743883
1104	60-61	0.3451585782247619
1104	62-63	0.27362909377402644
1104	64-65	0.3230295056362422
1104	66-67	0.36903239216834294
1104	68-69	0.39629375265426603
1104	70-71	0.25155053225523716
1104	72-73	0.18966336623428504
1104	74-75	0.28232035635098285
1104	76-77	0.3188135543180266
1104	78-79	0.30158113314838175
1104	80-81	0.26965200545154744
1104	82-83	0.27106555418829714
1104	84-85	0.259211927386815
1104	86-87	0.33324481466771516
1104	88-89	0.34356183783996386
1104	90-91	0.33447337081380013
1104	92-93	0.3451707926288705
1104	94-95	0.3351792010360555
1104	96-97	0.3769988972158096
1104	98-99	0.34418389395118965
1104	100	0.33815720756034295
1105	1	0.24521426806809288
1105	2	0.2530695579191935
1105	3	0.28466749209462705
1105	4	0.3104186373904625
1105	5	0.21927911397150268
1105	6	0.2556996577691919
1105	7	0.2708133811070965
1105	8	0.33620564823768007
1105	9	0.40969262618431657
1105	10-11	0.3806326164484588
1105	12-13	0.35236525295331234
1105	14-15	0.48534025847173723
1105	16-17	0.47038448052430226
1105	18-19	0.4772613673771815
1105	20-21	0.4860144655509373
1105	22-23	0.45212450209530175
1105	24-25	0.44222753895137856
1105	26-27	0.4805618547312278
1105	28-29	0.44887619508205745
1105	30-31	0.47089345984637276
1105	32-33	0.48863206034688034
1105	34-35	0.4442083401021648
1105	36-37	0.41210410149135157
1105	38-39	0.4364919099305169
1105	40-41	0.45651946857752534
1105	42-43	0.46153660536524654
1105	44-45	0.3729898768132216
1105	46-47	0.37206591347079154
1105	48-49	0.38635239907407737
1105	50-51	0.483553658871287
1105	52-53	0.5459875745632132
1105	54-55	0.5570051017259701
1105	56-57	0.5546278506518121
1105	58-59	0.511688371062732
1105	60-61	0.5334546899888792
1105	62-63	0.5039085681246576
1105	64-65	0.49080450707732126
1105	66-67	0.5223014381304978
1105	68-69	0.5608066280897575
1105	70-71	0.5399032010889471
1105	72-73	0.4999910045965237
1105	74-75	0.3799221249637874
1105	76-77	0.45997298259873176
1105	78-79	0.4098182484537922
1105	80-81	0.4477589615255546
1105	82-83	0.4694671695317183
1105	84-85	0.5290640296751903
1105	86-87	0.4720859600333043
1105	88-89	0.48598390061079044
1105	90-91	0.5555654262859555
1105	92-93	0.5009353113800046
1105	94-95	0.5850584429207011
1105	96-97	0.5721565148905796
1105	98-99	0.5419226415030991
1105	100	0.5778186671337338
1106	1	0.2338947821423858
1106	2	0.2825031735867043
1106	3	0.2522809279493572
1106	4	0.2400278014615509
1106	5	0.2738619695906124
1106	6	0.2509034510538086
1106	7	0.2396133294689875
1106	8	0.2075433768064059
1106	9	0.1952260727052817
1106	10-11	0.1758865135119052
1106	12-13	0.21471289804924965
1106	14-15	0.2472033201353625
1106	16-17	0.30193441208793814
1106	18-19	0.28727680590164084
1106	20-21	0.26199719006427813
1106	22-23	0.25108412120076906
1106	24-25	0.2575514649078343
1106	26-27	0.34857508911268553
1106	28-29	0.3205634067670857
1106	30-31	0.3165469967128516
1106	32-33	0.31927171153569134
1106	34-35	0.35093396556610656
1106	36-37	0.37192253745983805
1106	38-39	0.2892545222473686
1106	40-41	0.30221932630819026
1106	42-43	0.33023351559694447
1106	44-45	0.2729970458333213
1106	46-47	0.3204133882033062
1106	48-49	0.28454030358802385
1106	50-51	0.2845444121580556
1106	52-53	0.20295620599991793
1106	54-55	0.264438793580851
1106	56-57	0.2608865639211757
1106	58-59	0.22550786095652597
1106	60-61	0.22915264303524197
1106	62-63	0.23865897776405376
1106	64-65	0.3230329559712004
1106	66-67	0.3320162275279728
1106	68-69	0.26309129691542665
1106	70-71	0.2576897515839818
1106	72-73	0.22907286210814704
1106	74-75	0.24974070907437707
1106	76-77	0.24706797925759716
1106	78-79	0.26603378887886464
1106	80-81	0.2570119972484406
1106	82-83	0.25783436303758833
1106	84-85	0.24267498309137636
1106	86-87	0.20069056185640477
1106	88-89	0.2706891533597542
1106	90-91	0.21691797964754045
1106	92-93	0.25011904833887044
1106	94-95	0.20587222458144439
1106	96-97	0.2135039573492712
1106	98-99	0.11835936868405383
1106	100	0.19964328062831527
1107	1	0.2073894309113662
1107	2	0.2641748979081484
1107	3	0.2314565134936899
1107	4	0.210822676689979
1107	5	0.2363509264310366
1107	6	0.25693794098098266
1107	7	0.2699672391369674
1107	8	0.3268703851799515
1107	9	0.23333549907843576
1107	10-11	0.2870104961624733
1107	12-13	0.36310847021419335
1107	14-15	0.3451830635182773
1107	16-17	0.4563960875093258
1107	18-19	0.4227165045393946
1107	20-21	0.3515061143900482
1107	22-23	0.3653650878546557
1107	24-25	0.3004714220609017
1107	26-27	0.3971807859732124
1107	28-29	0.3442229609842542
1107	30-31	0.311724183808046
1107	32-33	0.33603389681341156
1107	34-35	0.3391521341454933
1107	36-37	0.39669689762087046
1107	38-39	0.3314834784512257
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2216	32-33	-0.8936053876584182
2216	34-35	-0.8692646337625547
2216	36-37	-0.8389131293876702
2216	38-39	-0.837745380765881
2216	40-41	-0.9303448385001047
2216	42-43	-0.914067473023465
2216	44-45	-0.8968262784106642
2216	46-47	-0.8026883858058298
2216	48-49	-0.9722919803878582
2216	50-51	-0.8435234462628358
2216	52-53	-0.9234871448081563
2216	54-55	-0.9770073261213454
2216	56-57	-0.8802643294295436
2216	58-59	-0.8377003350027081
2216	60-61	-0.7316513746468516
2216	62-63	-0.8044877054319599
2216	64-65	-0.894732450626357
2216	66-67	-1.0178508582128032
2216	68-69	-1.0300893194063931
2216	70-71	-1.0580312086549597
2216	72-73	-0.9931624588988157
2216	74-75	-1.062943705432783
2216	76-77	-0.9509712285115874
2216	78-79	-0.8842160559953598
2216	80-81	-0.8149946463029174
2216	82-83	-0.7538448315596682
2216	84-85	-0.7825671636127822
2216	86-87	-0.8308605156141446
2216	88-89	-0.9246810427680039
2216	90-91	-0.8775517406252291
2216	92-93	-0.9354357056553511
2216	94-95	-0.9655692040292543
2216	96-97	-1.0155506168238517
2216	98-99	-1.0236767020655329
2216	100	-1.0308050599806542
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78383.0
3	18501.0
4	15351.0
5	14410.0
6	8769.0
7	7691.0
8	8193.0
9	8362.0
10	7869.0
11	8760.0
12	10336.0
13	10951.0
14	10847.0
15	10780.0
16	10340.0
17	10233.0
18	9814.0
19	9033.0
20	8221.0
21	8752.0
22	11359.0
23	21173.0
24	37723.0
25	38134.0
26	39289.0
27	29342.0
28	30279.0
29	31941.0
30	36995.0
31	45132.0
32	59568.0
33	80761.0
34	116787.0
35	210624.0
36	358712.0
37	621433.0
38	690981.0
39	245719.0
40	2854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.145271136264256	22.355192108256812	12.195175890510106	22.30436086496883
2	16.124743290194584	23.89176253459237	36.99811117875915	22.985382996453897
3	17.854001523253746	30.88164090549494	27.574545109722187	23.68981246152913
4	12.119852526520397	20.42540529908682	35.934748715068785	31.519993459324
5	12.780521116297711	42.17506293557736	31.644943156097323	13.399472792027614
6	28.341164608911466	40.648324404126654	17.434453345914307	13.576057641047568
7	25.863908414483035	36.5451437172338	21.16162634926528	16.429321519017883
8	24.3769773643095	38.42099690323221	19.11039464522541	18.091631087232887
9	25.736110617805508	20.385792530630926	19.43153100688178	34.44656584468178
10-11	23.73396747489112	30.595777646577105	25.72880597184964	19.941448906682144
12-13	23.835947704096164	29.45838060690215	25.49110341033145	21.21456827867023
14-15	22.098329916680125	29.643258515441286	23.863524417957098	24.394887149921495
16-17	21.75157703285281	32.33011169406802	24.26635218713833	21.651959085940835
18-19	21.60049148874716	31.183315786546185	26.188965159519395	21.027227565187264
20-21	23.83707020702975	29.667920072429922	25.324068272303794	21.17094144823653
22-23	23.149746582397412	30.18934781574332	25.349014643469616	21.311890958389654
24-25	22.350608262559803	30.245958821901336	25.352599951347038	22.050832964191823
26-27	22.22830570412431	30.63027366956596	25.36623752430135	21.775183102008373
28-29	22.07755523552122	30.187873483532044	25.973025751892674	21.76154552905406
30-31	22.502162912369045	30.34023231454744	25.379422745327208	21.778182027756316
32-33	21.75159378662794	30.272798369656634	25.96617345786526	22.009434385850163
34-35	22.721201768394472	29.57466856006664	25.833952664553905	21.870177006984985
36-37	22.698600925746597	30.040892614332787	25.27003734751552	21.990469112405098
38-39	22.26154519397856	29.930837065515973	25.556996007910467	22.250621732595004
40-41	22.296208754718702	29.901853034544274	25.709153793624317	22.092784417112707
42-43	22.069999282938426	30.0841676154888	25.903263032259062	21.942570069313717
44-45	22.071004509446112	29.77457460489572	25.620023709942565	22.534397175715604
46-47	22.494154607857787	29.8002748959423	25.664655766883953	22.040914729315958
48-49	22.278500014408248	29.69353659460086	25.714682539416607	22.313280851574284
50-51	22.27484769143031	30.00889290383802	25.173753401854036	22.54250600287763
52-53	22.067653754420483	30.031125163433074	25.592731810258805	22.308489271887634
54-55	22.25077251657116	29.992557973088076	25.544765752066912	22.211903758273852
56-57	22.200310145885172	30.160313523446234	25.309978347421026	22.329397983247564
58-59	22.57770568442187	29.38226820649497	25.527710408986458	22.512315700096703
60-61	22.831558885163595	29.90783413226502	25.263871958268357	21.996735024303028
62-63	21.905845794232814	29.99158625413064	25.73520591394859	22.36736203768795
64-65	22.558154028847323	29.776082444657252	25.383963018386936	22.281800508108493
66-67	22.343923506283673	29.799587991162046	25.363422890079825	22.493065612474457
68-69	22.505580682495186	29.960776061669975	25.446772921342365	22.08687033449247
70-71	22.655175140614432	29.938258987897743	25.29288949679031	22.113676374697512
72-73	22.359035411449263	29.98610776966374	25.504724229510632	22.150132589376366
74-75	22.408559570728073	29.713088250175414	25.55786720421713	22.32048497487939
76-77	22.454515175904586	30.00368247977317	25.13049515447316	22.41130718984909
78-79	22.458083730006884	29.91512202444577	25.423066329536038	22.203727916011314
80-81	22.317854632184268	30.23523640581932	25.42204434925322	22.02486461274319
82-83	21.959249074813567	30.373892762645472	25.71265197995509	21.95420618258587
84-85	22.28078443619298	29.6718369661024	25.36929294154525	22.678085656159368
86-87	22.623643389942732	30.119298250316902	25.17262443082608	22.084433928914287
88-89	22.043976200752205	30.03100228663645	25.476695341212803	22.44832617139855
90-91	22.2653774168617	29.901657647580965	25.162017672705623	22.670947262851715
92-93	21.99425065315971	30.177620571925644	25.64775650487428	22.180372270040365
94-95	22.231638048920075	29.58580079930651	25.66925250063917	22.51330865113424
96-97	22.332507076516855	30.284710953920335	25.158238372438817	22.22454359712399
98-99	21.540267129530346	30.19731463113875	25.667857334334123	22.594560904996783
100	21.794846448644257	30.181438864783196	25.225085455235728	22.798629231336815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62047.0
1	42196.5
2	17668.5
3	9767.0
4	5088.5
5	3145.0
6	2629.0
7	3073.5
8	4004.0
9	4147.0
10	2853.0
11	1652.0
12	1393.5
13	1338.0
14	1307.5
15	1372.0
16	1547.0
17	1857.0
18	2207.0
19	2626.0
20	3436.5
21	4383.5
22	5488.5
23	6994.0
24	9249.0
25	11534.5
26	14128.0
27	17864.5
28	22114.5
29	27024.0
30	31474.5
31	35192.0
32	39613.0
33	44038.0
34	48466.0
35	55088.0
36	61863.0
37	68175.5
38	77037.0
39	84122.5
40	90170.5
41	95947.5
42	98833.0
43	104301.0
44	107215.0
45	105462.0
46	105768.5
47	104703.0
48	100373.0
49	101716.5
50	105644.0
51	101432.0
52	94674.5
53	89724.0
54	86161.5
55	84951.0
56	77956.0
57	67763.0
58	63364.5
59	61265.5
60	56585.5
61	48372.0
62	40527.5
63	33437.5
64	27296.5
65	23340.0
66	19991.0
67	16612.0
68	13460.5
69	10462.5
70	8458.0
71	7649.5
72	6082.5
73	4115.0
74	3214.0
75	2520.5
76	1676.0
77	1275.0
78	1007.0
79	712.0
80	462.0
81	295.0
82	241.0
83	190.0
84	138.0
85	104.0
86	90.5
87	76.0
88	58.0
89	42.0
90	41.0
91	44.0
92	37.5
93	23.5
94	19.5
95	20.5
96	12.0
97	13.0
98	18.0
99	17.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.696283543570873E-4
2	3.350755025629925E-5
3	3.350755025629925E-5
4	3.350755025629925E-5
5	3.350755025629925E-5
6	3.350755025629925E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.1727642589704737E-4
84-85	5.528745792289377E-4
86-87	0.0011057491584578753
88-89	0.003551800327167721
90-91	0.002764372896144688
92-93	0.0021109756661468527
94-95	0.0021779907666594515
96-97	0.0019936992402498055
98-99	0.0027476191210165386
100	0.003049187073323232
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2984402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.32184582414822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79801101890112	30.386243528224284
2	14.255554752640059	12.06642780757872
3	5.482647823112666	6.961073276336283
4	2.641907097603789	4.472415394660418
5	1.4988460918611695	3.1716966606937755
6	0.9480648199354305	2.407431188436368
7	0.6033177873798659	1.787346566631984
8	0.4483971668443892	1.5181596610538484
9	0.3251262002171678	1.2383946827083907
>10	1.7073403500276727	13.264598582133466
>50	0.158124191942988	4.6933008842901325
>100	0.11755596610426033	9.855085528795632
>500	0.010654222523960333	3.0474876692713204
>1k	0.004293492658887294	2.6517668611050285
>5k	7.950912331272766E-5	0.2067439324234413
>10k+	7.950912331272766E-5	2.2718277756569365
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67514	2.2622287480037877	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6144	0.2058703887747026	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3396	0.11379164067039226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.3507550256299255E-5	0.0
5	0.0	0.0	0.0	3.3507550256299255E-5	0.0
6	0.0	0.0	0.0	3.3507550256299255E-5	0.0
7	0.0	0.0	0.0	3.3507550256299255E-5	0.0
8	0.0	0.0	0.0	3.3507550256299255E-5	0.0
9	0.0	0.0	0.0	3.3507550256299255E-5	0.0
10-11	0.0	0.0	0.0	6.701510051259851E-5	0.0
12-13	0.0	0.0	0.0	1.3403020102519702E-4	0.0
14-15	1.6753775128149628E-5	0.0	0.0	2.010453015377955E-4	0.0
16-17	3.3507550256299255E-5	0.0	0.0	2.6806040205039404E-4	0.0
18-19	3.3507550256299255E-5	0.0	0.0	3.518292776911421E-4	0.0
20-21	3.3507550256299255E-5	0.0	0.0	5.361208041007881E-4	0.0
22-23	3.3507550256299255E-5	0.0	0.0	0.001306794459995671	0.0
24-25	3.3507550256299255E-5	0.0	0.0	0.002194744541787601	0.0
26-27	3.3507550256299255E-5	0.0	0.0	0.0033340012505017756	0.0
28-29	3.3507550256299255E-5	0.0	0.0	0.006198896797415362	0.0
30-31	3.3507550256299255E-5	0.0	0.0	0.013252236126366355	0.0
32-33	3.3507550256299255E-5	0.0	0.0	0.02554950707042818	0.0
34-35	3.3507550256299255E-5	0.0	0.0	0.040929472638069536	0.0
36-37	3.3507550256299255E-5	0.0	0.0	0.06609364288055028	0.0
38-39	3.3507550256299255E-5	0.0	0.0	0.09809335337531605	0.0
40-41	3.3507550256299255E-5	0.0	0.0	0.13255586881391984	0.0
42-43	3.3507550256299255E-5	0.0	0.0	0.16298072444663958	0.0
44-45	3.3507550256299255E-5	0.0	0.0	0.2025698950744571	0.0
46-47	3.3507550256299255E-5	0.0	0.0	0.25088778254404065	0.0
48-49	3.3507550256299255E-5	0.0	0.0	0.3006800022249013	0.0
50-51	3.3507550256299255E-5	0.0	0.0	0.35313607215113785	0.0
52-53	3.3507550256299255E-5	0.0	0.0	0.4041848249666097	0.0
54-55	3.3507550256299255E-5	0.0	0.0	0.4596063130905287	0.0
56-57	3.3507550256299255E-5	0.0	0.0	0.52559943332031	0.0
58-59	3.3507550256299255E-5	0.0	0.0	0.6018291101533908	0.0
60-61	3.3507550256299255E-5	0.0	0.0	0.6879100067618236	0.0
62-63	3.3507550256299255E-5	0.0	0.0	0.7739909033702564	0.0
64-65	3.3507550256299255E-5	0.0	0.0	0.8646623343638022	0.0
66-67	3.3507550256299255E-5	0.0	0.0	0.9669106239708993	0.0
68-69	3.3507550256299255E-5	0.0	0.0	1.090469715541003	0.0
70-71	3.3507550256299255E-5	0.0	0.0	1.2278506715918298	0.0
72-73	3.3507550256299255E-5	0.0	0.0	1.3730556406275025	0.0
74-75	3.3507550256299255E-5	0.0	0.0	1.5228008827229038	0.0
76-77	3.3507550256299255E-5	0.0	0.0	1.6812915954351995	0.0
78-79	3.3507550256299255E-5	0.0	0.0	1.8654825991940764	0.0
80-81	3.3507550256299255E-5	0.0	0.0	2.076379120507224	0.0
82-83	3.3507550256299255E-5	0.0	0.0	2.3016503808803237	0.0
84-85	3.3507550256299255E-5	0.0	0.0	2.5328692314239167	0.0
86-87	3.3507550256299255E-5	1.6753775128149628E-5	0.0	2.778094237974643	0.0
88	3.3507550256299255E-5	3.3507550256299255E-5	0.0	2.9771458402721884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4670	0.0	47.00012	1
GTATCAA	8330	0.0	38.98809	1
ATCAACG	10500	0.0	29.945288	3
TCAACGC	10540	0.0	29.653276	4
TACATGG	19130	0.0	28.425629	2
GTACATG	20140	0.0	28.260746	1
CAACGCA	11655	0.0	26.897085	5
AACGCAG	11900	0.0	26.461805	6
TATCAAC	12525	0.0	25.779272	2
ACGCAGA	12320	0.0	25.368956	7
ACATGGG	21040	0.0	24.951641	3
CGCAGAG	12480	0.0	24.893074	8
CATGGGG	12305	0.0	22.344257	4
GCAGAGT	13350	0.0	22.17946	9
CATGGGA	5885	0.0	20.20532	4
CATGGGT	3040	0.0	19.789192	4
CAGAGTA	13240	0.0	18.760685	10-11
GAGTACA	13845	0.0	18.501308	1
TAGCGTG	235	2.7996011E-6	17.999743	9
GTATAGA	840	0.0	17.904806	1
>>END_MODULE