FastQCFastQC Report
Wed 25 May 2016
SRR2431345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984402
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395341.3246874918325346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271670.9102996178128817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245650.8231129720459911No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170590.571605299822209No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110610.37062701338492604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82460.27630325941344364No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76120.2550594725509499No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46850.156982872950762No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.11811411465345488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91200.064.836411
GTATCAA197250.049.946771
ATCAACG276500.035.4733543
TCAACGC279350.035.1114464
TATCAAC284050.034.596662
CAACGCA289150.033.937125
AACGCAG297800.032.9987146
TATAACG2450.032.6105232
GTGGTAT27100.030.5264661
ACGCAGA327200.029.9474897
CGCAGAG330950.029.6081568
TGGTATC28850.028.5080282
GCAGAGT348300.028.07939
GTACATG197900.027.2902431
TACATGG189550.027.0753352
ACATGGG194850.025.4705563
AGTACTT255500.023.15807712-13
AGAGTAC345450.023.12764410-11
GTATAAC5900.022.3068981
CAGAGTA347000.022.23880210-11
Produced by FastQC (version 0.11.5)