##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431345_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2984402 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69891723702102 34.0 31.0 34.0 31.0 34.0 2 32.80787206281191 34.0 31.0 34.0 31.0 34.0 3 32.884005908051265 34.0 31.0 34.0 31.0 34.0 4 36.291701654133725 37.0 37.0 37.0 35.0 37.0 5 36.253660867403255 37.0 37.0 37.0 35.0 37.0 6 36.29565252938445 37.0 37.0 37.0 35.0 37.0 7 36.1574851511291 37.0 37.0 37.0 35.0 37.0 8 36.21497405510384 37.0 37.0 37.0 35.0 37.0 9 38.01479224313615 39.0 39.0 39.0 35.0 39.0 10-11 38.00730900193741 39.0 39.0 39.0 35.0 39.0 12-13 37.977888032510364 39.0 39.0 39.0 35.0 39.0 14-15 39.39812749756903 41.0 39.0 41.0 36.0 41.0 16-17 39.41374754473426 41.0 39.0 41.0 36.0 41.0 18-19 39.4952680637528 41.0 39.5 41.0 36.0 41.0 20-21 39.474130495824625 41.0 39.5 41.0 36.0 41.0 22-23 39.358217157072005 41.0 39.0 41.0 35.5 41.0 24-25 39.29677419462928 41.0 39.0 41.0 35.0 41.0 26-27 39.166677612466415 41.0 39.0 41.0 35.0 41.0 28-29 39.04797208955094 41.0 39.0 41.0 35.0 41.0 30-31 38.7874698180741 41.0 38.5 41.0 35.0 41.0 32-33 38.484782210975595 40.5 38.0 41.0 35.0 41.0 34-35 38.32956200270607 40.0 38.0 41.0 35.0 41.0 36-37 38.177424321522366 40.0 37.5 41.0 34.5 41.0 38-39 38.023638906554815 40.0 37.0 41.0 34.0 41.0 40-41 37.876958097468105 40.0 37.0 41.0 34.0 41.0 42-43 37.667445605518296 40.0 37.0 41.0 33.0 41.0 44-45 37.35986974945064 40.0 36.5 41.0 33.0 41.0 46-47 36.90579084185039 40.0 36.0 41.0 31.5 41.0 48-49 36.507580915707734 40.0 35.5 41.0 31.0 41.0 50-51 36.28909979285632 40.0 35.0 41.0 30.0 41.0 52-53 36.13239855086547 40.0 35.0 41.0 30.0 41.0 54-55 35.93751143445152 39.0 35.0 41.0 30.0 41.0 56-57 35.716798206139785 39.0 35.0 41.0 29.5 41.0 58-59 35.47871081040691 39.0 35.0 41.0 29.0 41.0 60-61 35.23027226224885 38.0 35.0 41.0 29.0 41.0 62-63 34.96327388200383 37.0 35.0 40.5 28.0 41.0 64-65 34.669688600932446 37.0 35.0 40.0 28.0 41.0 66-67 34.35731211813958 36.0 35.0 40.0 28.0 41.0 68-69 34.07994650184526 36.0 35.0 39.0 27.5 41.0 70-71 33.83166158580513 35.0 35.0 39.0 27.0 41.0 72-73 33.58715464605639 35.0 35.0 38.5 27.0 41.0 74-75 33.32443836319638 35.0 34.0 37.0 27.0 40.0 76-77 33.05685427097288 35.0 34.0 37.0 27.0 39.0 78-79 32.79484080897949 35.0 34.0 36.0 26.5 39.0 80-81 32.54852261860165 35.0 34.0 36.0 26.0 38.5 82-83 32.302037895699044 35.0 34.0 36.0 26.0 37.0 84-85 32.108616399533304 35.0 34.0 35.0 25.5 37.0 86-87 31.94570905662173 35.0 34.0 35.0 25.0 36.5 88-89 31.771928681189735 35.0 34.0 35.0 25.0 36.0 90-91 31.610118878086798 35.0 34.0 35.0 24.0 36.0 92-93 31.489298693674648 35.0 34.0 35.0 24.0 36.0 94-95 31.367075715671014 35.0 34.0 35.0 23.5 36.0 96-97 31.240275438764616 35.0 34.0 35.0 21.5 35.0 98-99 31.12605674436621 35.0 33.0 35.0 20.0 35.0 100 31.02017757661334 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17414610926066132 1101 2 0.18814913266247402 1101 3 0.19648305353386064 1101 4 0.1465691221704546 1101 5 0.14347041849049447 1101 6 0.1446677730872068 1101 7 0.16749481003390798 1101 8 0.1664937058609084 1101 9 0.2407914573446135 1101 10-11 0.2597142260689935 1101 12-13 0.25686431135683563 1101 14-15 0.29334568667167815 1101 16-17 0.3167859353665463 1101 18-19 0.2931138624816114 1101 20-21 0.2439219305199103 1101 22-23 0.24544813807325028 1101 24-25 0.2362782048375749 1101 26-27 0.222787122795701 1101 28-29 0.2648638585871126 1101 30-31 0.3334419808177529 1101 32-33 0.32868640829251916 1101 34-35 0.34375214022836786 1101 36-37 0.353053462319572 1101 38-39 0.3587117063009657 1101 40-41 0.40426197114806683 1101 42-43 0.46591483245943266 1101 44-45 0.4817853260815994 1101 46-47 0.4938729303266456 1101 48-49 0.5272411283102016 1101 50-51 0.5113834751767641 1101 52-53 0.5281810435489476 1101 54-55 0.5180472801357325 1101 56-57 0.45387152086116345 1101 58-59 0.5284530904355833 1101 60-61 0.5012688577617013 1101 62-63 0.41479919736272564 1101 64-65 0.43423284123483086 1101 66-67 0.4164809500328204 1101 68-69 0.32233564902549006 1101 70-71 0.32760666977300446 1101 72-73 0.32596555627148405 1101 74-75 0.3291594541256444 1101 76-77 0.33704631652051376 1101 78-79 0.2982206821479352 1101 80-81 0.31138377789334726 1101 82-83 0.4173834051290939 1101 84-85 0.33745882651689385 1101 86-87 0.36790865788339033 1101 88-89 0.37890161048841975 1101 90-91 0.36514583503949183 1101 92-93 0.45497642859324117 1101 94-95 0.4389206843242661 1101 96-97 0.446386045930371 1101 98-99 0.4655591616584971 1101 100 0.4898741494386556 1102 1 0.10795669601306201 1102 2 0.15882747662920593 1102 3 0.1988623797708584 1102 4 0.09388037246890946 1102 5 0.12284010526344247 1102 6 0.12271106183246872 1102 7 0.13697932636713261 1102 8 0.15766493405967452 1102 9 0.21719739760881396 1102 10-11 0.232709775590628 1102 12-13 0.22275346307289112 1102 14-15 0.2843191004079131 1102 16-17 0.2858002085476272 1102 18-19 0.2505535603043967 1102 20-21 0.2820116258439995 1102 22-23 0.31797941358090753 1102 24-25 0.30852212881018914 1102 26-27 0.3320591378101696 1102 28-29 0.30338978685750817 1102 30-31 0.3357630038447468 1102 32-33 0.3709180608275986 1102 34-35 0.3445909429580709 1102 36-37 0.3686072317609117 1102 38-39 0.3931685563728706 1102 40-41 0.4073187251584969 1102 42-43 0.4679533625522154 1102 44-45 0.5120259517583392 1102 46-47 0.42451848567860395 1102 48-49 0.4316984781239057 1102 50-51 0.42517454423552437 1102 52-53 0.3950616274694809 1102 54-55 0.43593553786924133 1102 56-57 0.46228071777609614 1102 58-59 0.4278377139356593 1102 60-61 0.3950723370751632 1102 62-63 0.3727906934230987 1102 64-65 0.42760432152557115 1102 66-67 0.413446059726148 1102 68-69 0.38303345515899423 1102 70-71 0.34145680553619684 1102 72-73 0.34642234804018557 1102 74-75 0.35338513196987265 1102 76-77 0.3458160910259025 1102 78-79 0.29066243446479234 1102 80-81 0.31313842688026483 1102 82-83 0.38320106600431103 1102 84-85 0.37718621736495805 1102 86-87 0.33824134710506826 1102 88-89 0.3525811815989002 1102 90-91 0.4072994029183121 1102 92-93 0.36044228512292165 1102 94-95 0.3264097177681009 1102 96-97 0.37756361807365124 1102 98-99 0.4552745448216413 1102 100 0.48281737877016795 1103 1 0.15143484214124925 1103 2 0.16682322936245697 1103 3 0.16062653759440337 1103 4 0.1476946511610251 1103 5 0.0904612365858597 1103 6 0.14171062719213978 1103 7 0.13918098387514277 1103 8 0.16661430236250396 1103 9 0.24408910423989738 1103 10-11 0.23719507331925627 1103 12-13 0.18051303215936798 1103 14-15 0.28299475545495056 1103 16-17 0.2822494793202921 1103 18-19 0.26097265088262134 1103 20-21 0.28364932949272514 1103 22-23 0.3168306803027363 1103 24-25 0.27022082667916436 1103 26-27 0.2308754887315203 1103 28-29 0.27189815586393706 1103 30-31 0.2729546650841428 1103 32-33 0.28622320633572684 1103 34-35 0.2953518044913892 1103 36-37 0.3347591571441413 1103 38-39 0.327481872843812 1103 40-41 0.3103330614048829 1103 42-43 0.289206896012864 1103 44-45 0.30035410337116275 1103 46-47 0.27648312192779656 1103 48-49 0.2396446511089252 1103 50-51 0.19741796301446612 1103 52-53 0.21414786846400347 1103 54-55 0.17004098886481245 1103 56-57 0.17316480925158828 1103 58-59 0.16765275313360206 1103 60-61 0.1061247541481265 1103 62-63 0.1497610552669073 1103 64-65 0.1206789451853254 1103 66-67 0.19937496918280573 1103 68-69 0.19909453979543912 1103 70-71 0.22868261380887134 1103 72-73 0.1873923509729778 1103 74-75 0.17960886021089095 1103 76-77 0.12906315031919746 1103 78-79 0.09118002133433833 1103 80-81 0.09392112828694366 1103 82-83 0.10394976036220527 1103 84-85 0.1426914488832196 1103 86-87 0.15196704316068121 1103 88-89 0.16594291801225936 1103 90-91 0.2373574436955579 1103 92-93 0.22600354485471286 1103 94-95 0.1905162289739728 1103 96-97 0.19918099643517095 1103 98-99 0.25694602833124236 1103 100 0.33887340560679746 1104 1 0.06567510552375211 1104 2 0.10666833277296206 1104 3 0.13049478317292085 1104 4 0.049260751193131114 1104 5 0.07829151065558193 1104 6 0.06929833906776395 1104 7 0.06638583755726302 1104 8 0.08806414738501189 1104 9 0.10949824050317147 1104 10-11 0.13650231555106984 1104 12-13 0.12806639153082244 1104 14-15 0.2494408284182228 1104 16-17 0.21992314117022715 1104 18-19 0.23366024336235114 1104 20-21 0.20707381178078776 1104 22-23 0.24090002013861778 1104 24-25 0.2431302153550945 1104 26-27 0.2053584251369429 1104 28-29 0.1957304882466886 1104 30-31 0.22863632168256487 1104 32-33 0.12700395997842406 1104 34-35 0.214264611350103 1104 36-37 0.24548517686081794 1104 38-39 0.23216630825348972 1104 40-41 0.20118270624835333 1104 42-43 0.2111820142755434 1104 44-45 0.18390572516822346 1104 46-47 0.19519100627968555 1104 48-49 0.18494824064415383 1104 50-51 0.21489650073444722 1104 52-53 0.19575294256249975 1104 54-55 0.19275125692317374 1104 56-57 0.21949101086053702 1104 58-59 0.16887824292222575 1104 60-61 0.14069575735851458 1104 62-63 0.16805013963568882 1104 64-65 0.2786505359879996 1104 66-67 0.26705065476717493 1104 68-69 0.18672443534038052 1104 70-71 0.23102897097051311 1104 72-73 0.19305847491086325 1104 74-75 0.13671067743797494 1104 76-77 0.11969066093853797 1104 78-79 0.10336770153509178 1104 80-81 0.07253182905431999 1104 82-83 0.13471878055665343 1104 84-85 0.06446632715714173 1104 86-87 0.011852165753435173 1104 88-89 0.024389426976149764 1104 90-91 0.10009452457278911 1104 92-93 0.13225958848935093 1104 94-95 0.1539653241286132 1104 96-97 0.12601030662655788 1104 98-99 0.15877229904196355 1104 100 0.09400469731792072 1105 1 0.004759048077808359 1105 2 0.03326238027365491 1105 3 0.0934377891853444 1105 4 -0.07356187820657567 1105 5 -0.031634825919404364 1105 6 -0.06610775099525767 1105 7 -0.4287609755173065 1105 8 -0.3728003932816364 1105 9 -0.32163738702550404 1105 10-11 -0.3525867926043773 1105 12-13 -0.2026292548557791 1105 14-15 -0.1492238845093965 1105 16-17 -0.10452897788198356 1105 18-19 -0.1318636140581333 1105 20-21 -0.09812746149122376 1105 22-23 -0.13548897665703663 1105 24-25 -0.16317741556086673 1105 26-27 -0.15582206654477204 1105 28-29 -0.22983430969753726 1105 30-31 -0.2974075266152809 1105 32-33 -0.27947518004223326 1105 34-35 -0.329288954822367 1105 36-37 -0.32360806934512 1105 38-39 -0.3274684015803331 1105 40-41 -0.3237298614231108 1105 42-43 -0.3344883345877392 1105 44-45 -0.31616414272325954 1105 46-47 -0.24819979471712372 1105 48-49 -0.18366152754983744 1105 50-51 -0.2114989147734363 1105 52-53 -0.23148322678346034 1105 54-55 -0.25864085176442586 1105 56-57 -0.20619837414886888 1105 58-59 -0.14921820929819063 1105 60-61 -0.14146083527158737 1105 62-63 -0.21625331034555728 1105 64-65 -0.1492568552217861 1105 66-67 -0.16261638254182742 1105 68-69 -0.21360485434348675 1105 70-71 -0.12125816517831822 1105 72-73 -0.06485343028267465 1105 74-75 -0.16322981372022127 1105 76-77 -0.09798318661022165 1105 78-79 -0.03673616186134154 1105 80-81 -0.06294630095899834 1105 82-83 -0.08167338521710121 1105 84-85 -0.10012574488391834 1105 86-87 -0.1271383782721074 1105 88-89 -0.06726240425042462 1105 90-91 -0.07026118904181544 1105 92-93 -0.11623850246589029 1105 94-95 -0.08372724768090478 1105 96-97 -0.14335976568907327 1105 98-99 -0.1267422964970173 1105 100 -0.138677213302973 1106 1 0.07952827705631194 1106 2 0.07552949425895861 1106 3 0.04418212393996157 1106 4 0.07570754309212901 1106 5 0.10143308612862256 1106 6 0.068071808885108 1106 7 0.09900540648723677 1106 8 0.08579515600021637 1106 9 0.04832732869771661 1106 10-11 0.1035080913606734 1106 12-13 0.08912332982728088 1106 14-15 0.10929859896614147 1106 16-17 0.1565192492537406 1106 18-19 0.1062043588339705 1106 20-21 0.09482393678514 1106 22-23 0.10329317348816858 1106 24-25 0.08119791941224719 1106 26-27 0.07689588621588683 1106 28-29 0.1194965641239989 1106 30-31 0.10932690801357126 1106 32-33 0.04355904025673141 1106 34-35 0.07884489369280345 1106 36-37 0.09917208723052084 1106 38-39 0.10000069094869701 1106 40-41 0.11805169033377894 1106 42-43 0.0672358544267837 1106 44-45 0.0306482358200455 1106 46-47 0.047591964031326484 1106 48-49 0.048284102178477895 1106 50-51 0.08303839772758437 1106 52-53 -0.037989258019230476 1106 54-55 -0.014061794932345606 1106 56-57 -0.06131255732764629 1106 58-59 -0.06506272251114353 1106 60-61 -0.0014873580005527742 1106 62-63 0.007600056474949213 1106 64-65 -0.015525610410072943 1106 66-67 -0.006943306788826931 1106 68-69 0.002588083827816945 1106 70-71 0.00865886994776588 1106 72-73 0.02152313003735884 1106 74-75 0.013859558829153684 1106 76-77 -0.06440267639820263 1106 78-79 -0.09234053670956399 1106 80-81 -0.09044055119324668 1106 82-83 -0.0052038286858007154 1106 84-85 -0.010455191779470852 1106 86-87 -0.03519453153993979 1106 88-89 -0.04859821755326976 1106 90-91 -0.03020824410888423 1106 92-93 -0.02704771817509055 1106 94-95 0.0071165380484607965 1106 96-97 -0.01127818911838574 1106 98-99 -0.08691450112148402 1106 100 -0.12898099457749268 1107 1 -0.059195121748871316 1107 2 -0.05272011942759036 1107 3 0.0271515479484421 1107 4 -0.05781067206374502 1107 5 -0.10064189524030098 1107 6 -0.044577785723525665 1107 7 0.001252643463139691 1107 8 0.009437758973405153 1107 9 0.09632243190532108 1107 10-11 0.08256613649179201 1107 12-13 0.051011537830845555 1107 14-15 0.053497177890839964 1107 16-17 0.09390810677621175 1107 18-19 0.06879549148388264 1107 20-21 0.08740166232485791 1107 22-23 0.08624164141973267 1107 24-25 0.050013078593849514 1107 26-27 0.04887292791364928 1107 28-29 0.04647634468783224 1107 30-31 0.08215187033803772 1107 32-33 0.09790393035273581 1107 34-35 0.12364774360456465 1107 36-37 0.1380069475139365 1107 38-39 0.09703764026384931 1107 40-41 0.12812520721463017 1107 42-43 0.07067688048983456 1107 44-45 0.18844118672146948 1107 46-47 0.27007864222694167 1107 48-49 0.24365524170828934 1107 50-51 0.3374504763037507 1107 52-53 0.29757406952477794 1107 54-55 0.27044729618181407 1107 56-57 0.2929482981830489 1107 58-59 0.35111891969784637 1107 60-61 0.25826550939770954 1107 62-63 0.29685939081819157 1107 64-65 0.3310569098418128 1107 66-67 0.3149571842954515 1107 68-69 0.3545459453387849 1107 70-71 0.3343194594954966 1107 72-73 0.331189931759603 1107 74-75 0.3271525981905441 1107 76-77 0.3977518673332838 1107 78-79 0.33209082241317134 1107 80-81 0.37524589198844893 1107 82-83 0.3962494429903032 1107 84-85 0.38164679497131715 1107 86-87 0.3112340559325446 1107 88-89 0.33276006407327685 1107 90-91 0.33381862115161454 1107 92-93 0.34757312498274473 1107 94-95 0.3213032095247499 1107 96-97 0.3484561929650418 1107 98-99 0.35404608912398317 1107 100 0.3408385747949616 1108 1 -0.12460081382011623 1108 2 -0.10991159356566271 1108 3 -0.09598845833014025 1108 4 -0.0702229190322754 1108 5 -0.24573396080319299 1108 6 -0.25061921386314623 1108 7 -0.24943526431348317 1108 8 -0.16884495160800128 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66-67 -0.09799595850602572 2214 68-69 -0.047723950010421845 2214 70-71 -0.12973751868793926 2214 72-73 -0.10604343077510237 2214 74-75 -0.0921886373930576 2214 76-77 -0.09987976364007523 2214 78-79 -0.1153116541849144 2214 80-81 -0.08035039521318765 2214 82-83 -0.05594522059401896 2214 84-85 -0.07775383315627238 2214 86-87 -0.0742487397404723 2214 88-89 -0.12062532648025126 2214 90-91 -0.06386838102255155 2214 92-93 -0.09598186934669428 2214 94-95 -0.11507885642475912 2214 96-97 -0.11609956650785236 2214 98-99 -0.07159196874345497 2214 100 -0.09393080816983357 2215 1 -0.08407515405531285 2215 2 -0.09337833532001838 2215 3 -0.09256773986767541 2215 4 -0.027983685192239705 2215 5 -0.023235644551817813 2215 6 -0.12646969565833643 2215 7 -0.1558815616511282 2215 8 -0.12367004667197534 2215 9 -0.12304199708073327 2215 10-11 -0.10870968943864057 2215 12-13 -0.06016569587182374 2215 14-15 -0.11735369621980851 2215 16-17 -0.13394120192599246 2215 18-19 -0.15357609089888058 2215 20-21 -0.17970533848093595 2215 22-23 -0.15460210497704452 2215 24-25 -0.13163556216368733 2215 26-27 -0.13920635961535766 2215 28-29 -0.12748814835181577 2215 30-31 -0.13698249186060707 2215 32-33 -0.23148918250566197 2215 34-35 -0.18620875737127562 2215 36-37 -0.19071227088798537 2215 38-39 -0.27231153875868586 2215 40-41 -0.24742720720664835 2215 42-43 -0.2313689464077484 2215 44-45 -0.2993731982988095 2215 46-47 -0.3144428750753434 2215 48-49 -0.31726121575110966 2215 50-51 -0.3650280670120978 2215 52-53 -0.35030952141074323 2215 54-55 -0.39456658230152186 2215 56-57 -0.4715224567185672 2215 58-59 -0.42810436487003756 2215 60-61 -0.3750222835738839 2215 62-63 -0.38987949316994275 2215 64-65 -0.3267078317861305 2215 66-67 -0.35448517903689947 2215 68-69 -0.43329872385608326 2215 70-71 -0.40772011556015286 2215 72-73 -0.3816864271125482 2215 74-75 -0.37273453008926083 2215 76-77 -0.33721486550714985 2215 78-79 -0.46845529680823006 2215 80-81 -0.414553100173805 2215 82-83 -0.38537000065301896 2215 84-85 -0.3760364918113517 2215 86-87 -0.4327303835838947 2215 88-89 -0.4442224861595996 2215 90-91 -0.44995658355699675 2215 92-93 -0.5534922024919098 2215 94-95 -0.566712559655322 2215 96-97 -0.5622842208738668 2215 98-99 -0.6229640566761248 2215 100 -0.5698035264645505 2216 1 -0.2197786959481789 2216 2 -0.2638114298167267 2216 3 -0.37706712571787193 2216 4 -0.2310966522494553 2216 5 -0.27746370716651825 2216 6 -0.19826068990541756 2216 7 -0.32407097069805957 2216 8 -0.3399922005986795 2216 9 -0.3339560068665577 2216 10-11 -0.35305663073395976 2216 12-13 -0.36476569188478436 2216 14-15 -0.48467837724485463 2216 16-17 -0.5498272526610819 2216 18-19 -0.5367814723730362 2216 20-21 -0.47859380683002684 2216 22-23 -0.532964946500833 2216 24-25 -0.5671651294693163 2216 26-27 -0.6417966451602268 2216 28-29 -0.6689942046383095 2216 30-31 -0.6207179057363916 2216 32-33 -0.6507447095086576 2216 34-35 -0.7336822946124784 2216 36-37 -0.7282033111064479 2216 38-39 -0.7335536563374347 2216 40-41 -0.7667906393156088 2216 42-43 -0.7558518161269419 2216 44-45 -0.7187070143971965 2216 46-47 -0.7953518259904939 2216 48-49 -0.7683405518525035 2216 50-51 -0.6848571864552397 2216 52-53 -0.7514301283647526 2216 54-55 -0.7137611564104347 2216 56-57 -0.6721636164367837 2216 58-59 -0.7434714130783107 2216 60-61 -0.723021543952612 2216 62-63 -0.7260976256396958 2216 64-65 -0.6507977936404643 2216 66-67 -0.6001184905477146 2216 68-69 -0.6527271773426406 2216 70-71 -0.6755763055382857 2216 72-73 -0.687062958180455 2216 74-75 -0.6316874688533431 2216 76-77 -0.5986268644033856 2216 78-79 -0.5567696277740808 2216 80-81 -0.6128827663835921 2216 82-83 -0.6593472754283738 2216 84-85 -0.6835866695091859 2216 86-87 -0.6721830702188498 2216 88-89 -0.6000442010335476 2216 90-91 -0.5591809546160071 2216 92-93 -0.73399045610687 2216 94-95 -0.6818544496999692 2216 96-97 -0.8327384155387989 2216 98-99 -0.775815318265412 2216 100 -0.803772524053592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 538.0 4 337.0 5 531.0 6 802.0 7 1326.0 8 2321.0 9 3273.0 10 3781.0 11 4588.0 12 7146.0 13 10673.0 14 10891.0 15 13741.0 16 18338.0 17 28625.0 18 34455.0 19 17994.0 20 10213.0 21 9160.0 22 9107.0 23 9187.0 24 10294.0 25 12340.0 26 15122.0 27 18813.0 28 23232.0 29 28727.0 30 35866.0 31 44200.0 32 55356.0 33 70900.0 34 102785.0 35 199533.0 36 322922.0 37 661337.0 38 848550.0 39 329698.0 40 7662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99694179456942 20.42268648233657 14.057457132009182 23.522914591084827 2 14.516217665192332 21.725371934057097 39.43734083902962 24.321069561720947 3 15.973051770395882 29.24571830280838 30.587198920784118 24.194031006011617 4 10.673716801704899 18.00907403311955 37.94705026907122 33.37015889610433 5 12.117067338783448 38.57724261007733 34.06786351168509 15.237826539454137 6 28.393963011685425 38.76331003665056 18.07836209733139 14.764364854332626 7 25.318103928358177 34.094166938636285 23.048570534398515 17.539158598607024 8 26.35874791666806 33.118091999670284 20.43310519159282 20.090054892068828 9 27.200658624407836 14.61666357280286 21.67218089252051 36.51049691026879 10-11 24.681158905536186 26.276939232717307 27.624093536996693 21.417808324749814 12-13 25.91887755067849 25.34236339474374 28.2331937855557 20.505565269022068 14-15 23.15636432357303 25.11029010166861 28.28432630724681 23.44901926751155 16-17 20.851178896140667 28.22042740890805 28.297679066023946 22.630714628927336 18-19 20.485410477542903 25.43197933790421 33.35108675037746 20.731523434175422 20-21 22.297364765202545 24.2993068628154 33.17197214048242 20.231356231499646 22-23 21.594514008922726 24.765015699127613 33.31899209942094 20.32147819252872 24-25 21.053401371599442 24.817174245132772 33.157178379968634 20.972246003299155 26-27 20.865418413119226 25.224158561417887 33.20326177973806 20.70716124572483 28-29 20.776189839540834 24.81306793755338 33.783813216377446 20.62692900652834 30-31 21.236108986601263 24.952682302351754 33.17921817776978 20.631990533277193 32-33 20.488063306720967 24.967424971881684 33.60323791674632 20.94127380465103 34-35 21.366213633485618 24.481417831837714 33.20415378431573 20.94821475036094 36-37 21.460008889947773 24.966528659693772 32.494803167073215 21.07865928328524 38-39 21.14749146245617 24.830325583546127 32.668245698305995 21.35393725569171 40-41 21.06850330891488 24.918435277578073 32.655158946703004 21.357902466804042 42-43 21.133606521809423 25.121980681791715 32.636355914356265 21.108056882042593 44-45 21.26459177867742 24.838861326919506 31.997486250103037 21.899060644300032 46-47 21.76343461241972 25.069040975900876 31.465042555512163 21.702481856167243 48-49 21.75717475243096 25.194424590668756 30.81294258422106 22.23545807267923 50-51 22.015252825978056 25.546607067165038 29.933872029728263 22.504268077128636 52-53 21.863419470677613 25.747030266212594 30.165170525872785 22.224379737237008 54-55 22.06838433076189 25.793040938954707 30.120167237276053 22.01840749300735 56-57 22.057471636035196 25.73225931781731 30.00067183212306 22.209597214024438 58-59 22.321237995384624 24.879262746312207 30.295157475288782 22.50434178301439 60-61 22.524750028523414 25.447423854217472 29.996526929032914 22.031299188226207 62-63 21.749734117588716 25.388352507470508 30.608409993023727 22.253503381917046 64-65 22.14570959274253 25.24256115630535 30.359515909719935 22.25221334123218 66-67 22.17197723813425 25.26381129733085 30.16984278966049 22.39436867487441 68-69 22.382141827376962 25.357465190023458 30.09469466286398 22.165698319735597 70-71 22.480657299963475 25.38072197109569 29.967697942251693 22.17092278668914 72-73 22.09450070550171 25.414563927568718 30.119544927105974 22.371390439823603 74-75 22.09367354891847 25.242624591074147 30.077474338573012 22.586227521434367 76-77 22.206760302681317 25.523297367337346 29.746635245425118 22.523307084556222 78-79 22.15446166940036 25.45640362355141 29.952619736526863 22.43651497052137 80-81 21.820987949066044 25.69613833652579 30.03771812150251 22.44515559290565 82-83 21.668274211050893 25.806909374873822 30.159191292085367 22.36562512198992 84-85 21.8578083251183 25.210563985784585 29.82395590889321 23.107671780203905 86-87 22.02438922641349 25.596859527310677 29.697829524042003 22.68092172223383 88-89 21.496319975338054 25.521427578153403 29.936066587252526 23.04618585925602 90-91 21.740017672195847 25.44119337249095 29.63199009231159 23.186798863001613 92-93 21.458566568363093 25.697761580381012 30.123516692228275 22.720155159027616 94-95 21.64521159532177 25.09618426096444 30.022792034247697 23.2358121094661 96-97 21.696454540458742 25.718722229891927 29.55535323912551 23.02946999052382 98-99 20.96132262155258 25.509249943580027 30.13268871873634 23.39673871613105 100 21.45554134031749 25.462038054095498 29.68543815287986 23.396982452707153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9389.0 1 5868.0 2 8760.5 3 11888.0 4 5321.5 5 2802.5 6 6399.5 7 16439.0 8 26597.0 9 25232.0 10 13771.0 11 5111.0 12 3357.5 13 3209.5 14 3438.5 15 3687.5 16 3934.0 17 4315.0 18 4703.5 19 5171.5 20 6010.5 21 6886.5 22 7900.0 23 9349.5 24 11413.5 25 13690.5 26 16192.0 27 19463.0 28 23617.0 29 28629.5 30 32855.0 31 36371.5 32 40846.5 33 44907.5 34 48837.5 35 55054.5 36 61740.0 37 68087.5 38 76549.0 39 83080.0 40 88482.0 41 94877.0 42 98356.0 43 103842.0 44 107895.5 45 106290.0 46 105605.5 47 105220.0 48 99879.0 49 99922.5 50 105849.5 51 102370.5 52 94275.5 53 88780.0 54 84549.5 55 81414.0 56 74076.5 57 64397.5 58 61161.5 59 60754.5 60 55723.5 61 46453.0 62 38587.0 63 32002.0 64 25483.0 65 20961.5 66 17519.5 67 14308.5 68 11390.5 69 8547.5 70 6868.0 71 6302.5 72 4852.5 73 3069.0 74 2453.0 75 1923.0 76 1184.0 77 883.0 78 718.0 79 454.5 80 253.5 81 116.5 82 54.5 83 32.5 84 16.5 85 12.0 86 8.0 87 4.0 88 3.0 89 2.5 90 0.5 91 0.5 92 1.0 93 1.0 94 4.0 95 6.0 96 4.5 97 8.0 98 7.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010018757526633476 2 6.70151005125985E-5 3 4.355981533318903E-4 4 0.0020774681158905535 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.6753775128149624E-5 24-25 1.0052265076889775E-4 26-27 6.366434548696858E-4 28-29 9.717189574326783E-4 30-31 0.00179265393871201 32-33 0.004607288160241147 34-35 0.006148635472030913 36-37 0.004439750408959651 38-39 0.0027978804464009873 40-41 0.0038701220546025635 42-43 5.193670289726384E-4 44-45 5.36120804100788E-4 46-47 0.004138182456652957 48-49 0.0027811266712728377 50-51 0.0012397793594830723 52-53 5.0261325384448876E-5 54-55 6.533972299978355E-4 56-57 8.54442531535631E-4 58-59 9.717189574326783E-4 60-61 3.853368279474414E-4 62-63 0.0 64-65 0.0 66-67 8.209349812793316E-4 68-69 6.869047802541346E-4 70-71 0.0024125436184535463 72-73 3.350755025629925E-5 74-75 0.001976945465121656 76-77 3.015679523066933E-4 78-79 0.0012397793594830723 80-81 9.214576320482294E-4 82-83 0.0012230255843549227 84-85 0.0012062718092267731 86-87 3.518292776911421E-4 88-89 0.0015748548620460648 90-91 0.0017759001635838604 92-93 0.0036523229779366184 94-95 8.711963066637806E-4 96-97 0.002613588919991342 98-99 0.0028984030971698853 100 0.004154936231781107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2984402.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.94853686025865 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34471765083289 25.935820369766017 2 15.843964070137325 12.025105090564397 3 6.087524987912278 6.93037999174604 4 3.0281571595448726 4.596565343505789 5 1.7050027208109646 3.2351179298768087 6 1.0716108218438138 2.439963766355522 7 0.7027035498279172 1.8666600093735517 8 0.5059992273952795 1.5361544265657727 9 0.3756913204183671 1.283124232887808 >10 1.9845972305352713 13.8157725367595 >50 0.1882161865267315 4.968098999145757 >100 0.14271642980822782 10.694124167138966 >500 0.013262947504293963 3.3824408428486405 >1k 0.00521675935168215 2.752700056200404 >5k 1.7683930005702204E-4 0.5320987529099442 >10k+ 4.420982501425551E-4 4.005873484355316 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39534 1.3246874918325346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27167 0.9102996178128817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24565 0.8231129720459911 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17059 0.571605299822209 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11061 0.37062701338492604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8246 0.27630325941344364 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7612 0.2550594725509499 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4685 0.156982872950762 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3525 0.11811411465345488 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.3507550256299255E-5 0.0 10-11 0.0 0.0 0.0 3.3507550256299255E-5 0.0 12-13 0.0 0.0 0.0 3.3507550256299255E-5 0.0 14-15 0.0 0.0 0.0 8.376887564074813E-5 0.0 16-17 0.0 0.0 0.0 1.6753775128149626E-4 0.0 18-19 0.0 0.0 0.0 2.1779907666594515E-4 0.0 20-21 0.0 0.0 0.0 4.0209060307559106E-4 0.0 22-23 0.0 0.0 0.0 0.001122502933586025 0.0 24-25 0.0 0.0 0.0 0.001976945465121656 0.0 26-27 0.0 0.0 0.0 0.0030659408484513817 0.0 28-29 0.0 0.0 0.0 0.0059643439456212675 0.0 30-31 0.0 0.0 0.0 0.01298417572431596 0.0 32-33 0.0 0.0 0.0 0.02546573819478743 0.0 34-35 0.0 0.0 0.0 0.04096298018832584 0.0 36-37 0.0 0.0 0.0 0.06637845705772882 0.0 38-39 0.0 0.0 0.0 0.09856245907890426 0.0 40-41 0.0 0.0 0.0 0.13339355757032734 0.0 42-43 0.0 0.0 0.0 0.1638686745284315 0.0 44-45 0.0 0.0 0.0 0.20387668953445282 0.0 46-47 0.0 0.0 0.0 0.25234536098018967 0.0 48-49 0.0 0.0 0.0 0.30213758066105034 0.0 50-51 0.0 0.0 0.0 0.3554480931188225 0.0 52-53 0.0 0.0 0.0 0.4072005044896767 0.0 54-55 0.0 0.0 0.0 0.46330889739384973 0.0 56-57 0.0 0.0 0.0 0.5303575054567046 0.0 58-59 0.0 0.0 0.0 0.607441624821321 0.0 60-61 0.0 0.0 0.0 0.6945780092628272 0.0 62-63 0.0 0.0 0.0 0.7818651776804868 0.0 64-65 0.0 0.0 0.0 0.8733575436553118 0.0 66-67 0.0 0.0 0.0 0.9765272908944573 0.0 68-69 0.0 0.0 0.0 1.1017952675276321 1.6753775128149628E-5 70-71 0.0 0.0 0.0 1.2414379832207592 3.3507550256299255E-5 72-73 0.0 0.0 0.0 1.389223033626167 3.3507550256299255E-5 74-75 0.0 0.0 0.0 1.541816417493354 3.3507550256299255E-5 76-77 0.0 0.0 0.0 1.7021165379194896 3.3507550256299255E-5 78-79 0.0 0.0 0.0 1.8893734825268178 3.3507550256299255E-5 80-81 0.0 0.0 0.0 2.1033191909132887 3.3507550256299255E-5 82-83 0.0 0.0 0.0 2.332024975187659 3.3507550256299255E-5 84-85 0.0 0.0 0.0 2.5669631638097012 3.3507550256299255E-5 86-87 0.0 0.0 0.0 2.8167619509704123 3.3507550256299255E-5 88 0.0 0.0 0.0 3.0209067009069153 3.3507550256299255E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9120 0.0 64.83641 1 GTATCAA 19725 0.0 49.94677 1 ATCAACG 27650 0.0 35.473354 3 TCAACGC 27935 0.0 35.111446 4 TATCAAC 28405 0.0 34.59666 2 CAACGCA 28915 0.0 33.93712 5 AACGCAG 29780 0.0 32.998714 6 TATAACG 245 0.0 32.610523 2 GTGGTAT 2710 0.0 30.526466 1 ACGCAGA 32720 0.0 29.947489 7 CGCAGAG 33095 0.0 29.608156 8 TGGTATC 2885 0.0 28.508028 2 GCAGAGT 34830 0.0 28.0793 9 GTACATG 19790 0.0 27.290243 1 TACATGG 18955 0.0 27.075335 2 ACATGGG 19485 0.0 25.470556 3 AGTACTT 25550 0.0 23.158077 12-13 AGAGTAC 34545 0.0 23.127644 10-11 GTATAAC 590 0.0 22.306898 1 CAGAGTA 34700 0.0 22.238802 10-11 >>END_MODULE