FastQCFastQC Report
Wed 25 May 2016
SRR2431344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936241
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224500.5703410944604256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.046.8343961
GTATCAA78700.038.1611251
TCAACGC89150.032.5808334
ATCAACG90100.032.4981233
CAACGCA98200.029.6739445
AACGCAG103050.028.1861346
TATCAAC108750.027.0977572
GTACATG186550.025.0177961
ACGCAGA115150.024.8569837
TACATGG182100.024.5193372
CGCAGAG116700.024.4462878
ACATGGG188150.022.9315553
GCAGAGT131750.020.4408539
GTGGTAT23450.019.4412381
CATGGGT35650.019.1162854
CATGGGG108600.018.9989574
TGGTATC22350.018.2951852
CATGGGA58000.017.6654034
CAGAGTA127600.017.66171810-11
AGAGTAC120500.017.37623610-11
Produced by FastQC (version 0.11.5)