##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431344_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3936241 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.077109607872078 33.0 31.0 34.0 28.0 34.0 2 31.23801083317815 34.0 31.0 34.0 28.0 34.0 3 31.30404439159086 34.0 31.0 34.0 28.0 34.0 4 34.56827643429353 37.0 35.0 37.0 32.0 37.0 5 34.66127150243087 37.0 35.0 37.0 32.0 37.0 6 34.72821379585244 37.0 35.0 37.0 32.0 37.0 7 34.65112883078043 37.0 35.0 37.0 32.0 37.0 8 34.64380001122899 37.0 35.0 37.0 32.0 37.0 9 36.142700103982456 39.0 37.0 39.0 32.0 39.0 10-11 36.14987560466953 39.0 37.0 39.0 32.0 39.0 12-13 36.08413534638758 39.0 37.0 39.0 32.0 39.0 14-15 37.4522765501401 41.0 38.0 41.0 31.5 41.0 16-17 37.447941449723224 41.0 38.0 41.0 31.5 41.0 18-19 37.458996032001096 41.0 38.0 41.0 31.0 41.0 20-21 37.49046539579258 41.0 38.0 41.0 31.0 41.0 22-23 37.48301094877067 41.0 38.0 41.0 31.0 41.0 24-25 37.49519135134256 41.0 38.0 41.0 31.0 41.0 26-27 37.486715244315576 41.0 38.0 41.0 31.0 41.0 28-29 37.453166485487046 40.0 38.0 41.0 31.0 41.0 30-31 37.38043694479073 40.0 38.0 41.0 30.0 41.0 32-33 37.30853065145148 40.0 38.0 41.0 30.0 41.0 34-35 37.24312154667359 40.0 38.0 41.0 30.0 41.0 36-37 37.1480834125756 40.0 38.0 41.0 30.0 41.0 38-39 37.046003153770314 40.0 38.0 41.0 30.0 41.0 40-41 36.95671606489542 40.0 38.0 41.0 30.0 41.0 42-43 36.8293806197334 40.0 37.0 41.0 30.0 41.0 44-45 36.692416699079146 40.0 37.0 41.0 30.0 41.0 46-47 36.555834868850766 40.0 37.0 41.0 29.0 41.0 48-49 36.412176871284046 40.0 36.0 41.0 29.0 41.0 50-51 36.2628168600449 40.0 36.0 41.0 29.0 41.0 52-53 36.09825998967035 40.0 35.5 41.0 29.0 41.0 54-55 35.92343482017488 39.5 35.0 41.0 28.0 41.0 56-57 35.72044521664197 39.0 35.0 41.0 28.0 41.0 58-59 35.491816049881095 39.0 35.0 41.0 28.0 41.0 60-61 35.30666707145218 39.0 35.0 41.0 28.0 41.0 62-63 35.14948386036322 38.5 35.0 41.0 28.0 41.0 64-65 34.89905953421043 38.0 34.5 40.0 27.0 41.0 66-67 34.59499164304218 37.5 34.0 40.0 26.5 41.0 68-69 34.28489223601909 37.0 34.0 40.0 26.0 41.0 70-71 34.040921274891446 36.5 34.0 39.5 26.0 41.0 72-73 33.79472331597583 36.0 34.0 39.0 26.0 41.0 74-75 33.45382866547043 35.5 34.0 39.0 26.0 41.0 76-77 33.110143408393945 35.0 33.5 38.5 26.0 40.0 78-79 32.77073113663518 35.0 33.0 37.0 25.5 39.0 80-81 32.43091645049172 35.0 33.0 37.0 25.0 39.0 82-83 32.10254072349737 35.0 33.0 36.0 24.5 39.0 84-85 31.81165761954108 35.0 33.0 36.0 24.0 37.0 86-87 31.550881030912485 35.0 33.0 36.0 23.5 37.0 88-89 31.30318011524193 35.0 32.0 35.0 23.0 37.0 90-91 31.09666887774402 35.0 32.0 35.0 21.5 36.0 92-93 30.896896937966957 35.0 32.0 35.0 20.0 36.0 94-95 30.681671803123844 35.0 32.0 35.0 18.5 36.0 96-97 30.49598792350367 35.0 32.0 35.0 17.5 36.0 98-99 30.37569523817266 35.0 32.0 35.0 6.0 35.0 100 30.331225400070778 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4097429918465174 1101 2 0.4763962221068958 1101 3 0.4891537767544136 1101 4 0.5036709064016733 1101 5 0.44646184858913784 1101 6 0.4722919309725171 1101 7 0.5674844959132272 1101 8 0.4989319247558086 1101 9 0.5885004037654369 1101 10-11 0.5898922779539504 1101 12-13 0.5834434788519545 1101 14-15 0.7068158376666105 1101 16-17 0.617967349080061 1101 18-19 0.6595863612642674 1101 20-21 0.6163783265508513 1101 22-23 0.6197662502820194 1101 24-25 0.6768306050835378 1101 26-27 0.6811658351277288 1101 28-29 0.6591211689442673 1101 30-31 0.7146536961896999 1101 32-33 0.6643837335806069 1101 34-35 0.6867788330293507 1101 36-37 0.7089300075597365 1101 38-39 0.6282576463172234 1101 40-41 0.6344913581449916 1101 42-43 0.6194958612375459 1101 44-45 0.6492751302384221 1101 46-47 0.6590337050035942 1101 48-49 0.6128908429729734 1101 50-51 0.5829178371760548 1101 52-53 0.5851337945128421 1101 54-55 0.5968477007489525 1101 56-57 0.4738807818213502 1101 58-59 0.5665370746540717 1101 60-61 0.5612540979857883 1101 62-63 0.5430299324587509 1101 64-65 0.5631888026408376 1101 66-67 0.4759830466294517 1101 68-69 0.5344728254903401 1101 70-71 0.5127390332843049 1101 72-73 0.4794258458249345 1101 74-75 0.5143136797827808 1101 76-77 0.5030352609637205 1101 78-79 0.5346042023328366 1101 80-81 0.5395362184035051 1101 82-83 0.507542807624084 1101 84-85 0.49544088235687056 1101 86-87 0.48977425180114054 1101 88-89 0.44155294906621734 1101 90-91 0.5016520763799157 1101 92-93 0.5251049412995314 1101 94-95 0.5448027356950043 1101 96-97 0.5369318432204935 1101 98-99 0.5599342875402868 1101 100 0.6085862535809596 1102 1 0.05886409711030893 1102 2 0.14283990964820248 1102 3 0.16463221176644538 1102 4 0.16913812332581557 1102 5 0.11613736915021633 1102 6 0.11347521664277593 1102 7 0.0923010533864499 1102 8 0.15369899089321848 1102 9 0.20237936437619908 1102 10-11 0.24183610481541962 1102 12-13 0.2133469758591886 1102 14-15 0.20196629244048836 1102 16-17 0.19728068963587475 1102 18-19 0.10242093057601664 1102 20-21 0.14664521104789685 1102 22-23 0.09220946000629482 1102 24-25 0.1711047276285882 1102 26-27 0.19118245648245136 1102 28-29 0.20750498065329737 1102 30-31 0.1977493332361533 1102 32-33 0.17594934535271278 1102 34-35 0.16061987111920928 1102 36-37 0.22012991199633092 1102 38-39 0.1645437862454031 1102 40-41 0.20083053291666886 1102 42-43 0.16644188629032186 1102 44-45 0.14799976513612023 1102 46-47 0.15542870684588195 1102 48-49 0.08965726473036995 1102 50-51 0.09063724507637261 1102 52-53 0.04549968368284851 1102 54-55 0.06187321193358741 1102 56-57 0.13050582758734208 1102 58-59 0.08478796729316684 1102 60-61 0.1577103702855922 1102 62-63 0.12420151148227632 1102 64-65 0.1025025353379263 1102 66-67 0.11879768209271901 1102 68-69 0.0976534348934166 1102 70-71 0.09507050280333829 1102 72-73 0.10000375724423805 1102 74-75 0.09112584164989812 1102 76-77 0.10942624821110059 1102 78-79 0.09081380482452772 1102 80-81 0.10590444786750908 1102 82-83 0.050693523048970235 1102 84-85 0.058032001819281476 1102 86-87 0.07600950072091095 1102 88-89 0.13786557211995643 1102 90-91 0.10454876772991284 1102 92-93 0.18879954697558787 1102 94-95 0.13328482150354048 1102 96-97 0.1072686589323375 1102 98-99 0.06531011742706738 1102 100 0.11782347796096815 1103 1 0.10782872433249224 1103 2 0.129437406075521 1103 3 0.20368594441936239 1103 4 0.18886523954772372 1103 5 0.10167845437380407 1103 6 0.1003610934448318 1103 7 0.1657900710715552 1103 8 0.18730543891419416 1103 9 0.2491349568772847 1103 10-11 0.2232540434088719 1103 12-13 0.17446289758598255 1103 14-15 0.1906667976192722 1103 16-17 0.155860967907536 1103 18-19 0.20549568369521864 1103 20-21 0.13727355442579636 1103 22-23 0.16186717507930126 1103 24-25 0.19886948467325283 1103 26-27 0.21941858972239459 1103 28-29 0.2411892323652367 1103 30-31 0.19770841093208702 1103 32-33 0.146917856986704 1103 34-35 0.19303822534985215 1103 36-37 0.21279930339571962 1103 38-39 0.17787118007190372 1103 40-41 0.1712453106149141 1103 42-43 0.13456341142227046 1103 44-45 0.10827119931535378 1103 46-47 0.004768497949712014 1103 48-49 0.0322326887701081 1103 50-51 0.0038719543740697304 1103 52-53 -0.03501584755024112 1103 54-55 0.042516962109971246 1103 56-57 -0.060998432693907034 1103 58-59 0.03410864421734772 1103 60-61 -0.021078214059251366 1103 62-63 -0.0226942944305506 1103 64-65 -0.026503168128023447 1103 66-67 0.07253200269267523 1103 68-69 0.05010229484164341 1103 70-71 0.009163746646393633 1103 72-73 0.056498344980887794 1103 74-75 0.0640545829619299 1103 76-77 0.1516004027614386 1103 78-79 0.11921202374217899 1103 80-81 0.04432908027232685 1103 82-83 0.10944082589711002 1103 84-85 0.04746942917898522 1103 86-87 0.1083800053609103 1103 88-89 0.08152261498959845 1103 90-91 0.02402316627467016 1103 92-93 0.12110898886719923 1103 94-95 0.06484598816122755 1103 96-97 0.044528096077474544 1103 98-99 0.07921985231724449 1103 100 0.05970636576714483 1104 1 0.04749918884591864 1104 2 0.12043192998194385 1104 3 0.1538453287593704 1104 4 0.15994552960082586 1104 5 0.0852645068782536 1104 6 0.13983629449376878 1104 7 0.15148823463739092 1104 8 0.17498861734424054 1104 9 0.18048032889991816 1104 10-11 0.17548620681879612 1104 12-13 0.18709595573348992 1104 14-15 0.28221005114687614 1104 16-17 0.2583813459611761 1104 18-19 0.18152399352143078 1104 20-21 0.21843093702371874 1104 22-23 0.13285848858673432 1104 24-25 0.1808182183457987 1104 26-27 0.21860659817623684 1104 28-29 0.2656915437911209 1104 30-31 0.26630377236593006 1104 32-33 0.2939259247255137 1104 34-35 0.2716834400445194 1104 36-37 0.2709756003385948 1104 38-39 0.17694785274809988 1104 40-41 0.14981517607893835 1104 42-43 0.1269307185113533 1104 44-45 0.11343765512694404 1104 46-47 0.13993323193163576 1104 48-49 0.1045188120506566 1104 50-51 0.03370820867940694 1104 52-53 0.010753638436028723 1104 54-55 -0.041441217378945794 1104 56-57 0.008878341389149114 1104 58-59 -0.05560537245830943 1104 60-61 -0.005947459357678042 1104 62-63 0.0064015424247116925 1104 64-65 0.006741449542921885 1104 66-67 0.01738445324608051 1104 68-69 0.052561718283449466 1104 70-71 0.01795508360262943 1104 72-73 -0.019220204311622524 1104 74-75 0.014849156712401168 1104 76-77 0.041696812946071304 1104 78-79 0.04681060539649451 1104 80-81 0.05660722973205168 1104 82-83 0.1295426962154025 1104 84-85 0.09647454310517745 1104 86-87 0.09589772240505923 1104 88-89 0.06496822021119186 1104 90-91 0.0411292371207459 1104 92-93 0.07273818962516998 1104 94-95 0.06813156757685945 1104 96-97 0.15342762286589462 1104 98-99 0.07071137422438056 1104 100 0.07067638506023144 1105 1 0.04823272994325478 1105 2 0.05199556608179634 1105 3 0.056038274359831775 1105 4 0.06902252081500393 1105 5 -0.02685731233170685 1105 6 0.056981201488397915 1105 7 0.05270036200075623 1105 8 0.08548749021096569 1105 9 0.13844934144424315 1105 10-11 0.1352629819756075 1105 12-13 0.19051524111322493 1105 14-15 0.19883154013076165 1105 16-17 0.189834601910448 1105 18-19 0.12296794084119256 1105 20-21 0.09758419401842389 1105 22-23 0.12074230496695293 1105 24-25 0.11904369199977793 1105 26-27 0.07362848051151616 1105 28-29 0.11101979954609931 1105 30-31 0.1211360288260579 1105 32-33 0.18964988943422156 1105 34-35 0.1560544031796809 1105 36-37 0.13515146110946574 1105 38-39 0.17295559030064567 1105 40-41 0.07794727419086911 1105 42-43 0.03466440325680509 1105 44-45 0.02050259739445437 1105 46-47 0.061544184129658674 1105 48-49 0.031235017658083564 1105 50-51 0.049074244223980656 1105 52-53 0.10819047814580074 1105 54-55 0.10939599388963472 1105 56-57 0.1148870996875857 1105 58-59 -0.016519887626408547 1105 60-61 0.017212713228538234 1105 62-63 0.012260696986821529 1105 64-65 0.01777675195877748 1105 66-67 0.0289916050020409 1105 68-69 0.038343419903114295 1105 70-71 -0.006979446475256168 1105 72-73 -0.006608491080328349 1105 74-75 0.002014930630267031 1105 76-77 -0.012588017096689441 1105 78-79 -0.027441754624994985 1105 80-81 -0.09939660466377376 1105 82-83 -0.049426792991944524 1105 84-85 -0.005032738007233917 1105 86-87 0.006625493499271329 1105 88-89 0.006174095086887377 1105 90-91 0.04164430385128881 1105 92-93 -0.05342298502323217 1105 94-95 0.00957618543736416 1105 96-97 0.029938675008022386 1105 98-99 0.029358303463222768 1105 100 -0.017674429422751103 1106 1 0.0869064539639055 1106 2 0.16172489337023066 1106 3 0.14648806285942229 1106 4 0.1591134608070206 1106 5 0.10684348941365585 1106 6 0.09142191835255176 1106 7 0.07532572612959854 1106 8 0.15155408814118232 1106 9 0.19294547418322594 1106 10-11 0.1422196990018776 1106 12-13 0.15764243003128087 1106 14-15 0.16586473267517476 1106 16-17 0.16899422634652694 1106 18-19 0.1769168039006388 1106 20-21 0.21959581409519302 1106 22-23 0.1719779117186846 1106 24-25 0.15493993885396407 1106 26-27 0.23536573571286823 1106 28-29 0.20803511050143442 1106 30-31 0.22855953167247378 1106 32-33 0.22363908953364842 1106 34-35 0.21232485670918777 1106 36-37 0.25651620276982356 1106 38-39 0.255211628368059 1106 40-41 0.23618806993658126 1106 42-43 0.1968554458736591 1106 44-45 0.18832656763504474 1106 46-47 0.2038216194390401 1106 48-49 0.19009124544908218 1106 50-51 0.17536796892496653 1106 52-53 0.1640479731313036 1106 54-55 0.11236797585844016 1106 56-57 0.12527584109842138 1106 58-59 0.1237839237337468 1106 60-61 0.07510351172044949 1106 62-63 0.14938482609585435 1106 64-65 0.1284358464763713 1106 66-67 0.13232845056086262 1106 68-69 0.1342674903693819 1106 70-71 0.14696399272781946 1106 72-73 0.09480412789427817 1106 74-75 0.1607685935541241 1106 76-77 0.1189384437393386 1106 78-79 0.12121411008465799 1106 80-81 0.15037412387153637 1106 82-83 0.15119308610146476 1106 84-85 0.1574517755118343 1106 86-87 0.1352109046280141 1106 88-89 0.09808763933026299 1106 90-91 0.04131707308470922 1106 92-93 0.08396406830870262 1106 94-95 0.021282889675159566 1106 96-97 -0.005627042209585653 1106 98-99 0.008310955938920728 1106 100 0.05332162281523978 1107 1 -0.07831121644980499 1107 2 0.012074293887724963 1107 3 -0.04091943617479643 1107 4 -0.042066943432146786 1107 5 -0.05195139248184688 1107 6 0.002236226259661578 1107 7 -0.012533841263575596 1107 8 -0.03349810848121848 1107 9 -0.06541715611802346 1107 10-11 -0.022373692576501014 1107 12-13 0.038203033871702985 1107 14-15 0.020073416401771738 1107 16-17 0.04116307736111935 1107 18-19 0.046130432303314706 1107 20-21 -0.048709275273722596 1107 22-23 0.026822761802065997 1107 24-25 -0.0060484888912810675 1107 26-27 0.0016178615429822685 1107 28-29 -0.020215133877840685 1107 30-31 -0.03206704347169875 1107 32-33 0.017467816611002718 1107 34-35 -0.029848212662813012 1107 36-37 0.020818343728926436 1107 38-39 0.05055418119108879 1107 40-41 0.022204906574508243 1107 42-43 0.07025518186157598 1107 44-45 0.06510723144591424 1107 46-47 -0.01608151692555282 1107 48-49 0.03985038041304989 1107 50-51 0.02596901190590728 1107 52-53 0.054954260259982846 1107 54-55 0.016454301846749786 1107 56-57 0.003907215654940899 1107 58-59 -0.05186837704719238 1107 60-61 -0.08206430214912785 1107 62-63 -0.009214971042382558 1107 64-65 -0.0043196924171979845 1107 66-67 0.036697996430902435 1107 68-69 0.024445162508499152 1107 70-71 0.03158430567284398 1107 72-73 0.015582170614507618 1107 74-75 0.0694256144549783 1107 76-77 0.06448506990977876 1107 78-79 0.030674686838786158 1107 80-81 0.01899212020885699 1107 82-83 0.05888654536386895 1107 84-85 -0.0027553997555251897 1107 86-87 0.053422435270100266 1107 88-89 0.09016117449771954 1107 90-91 0.06793443888861006 1107 92-93 0.08350730583774535 1107 94-95 0.15912404219383802 1107 96-97 0.09460176403333165 1107 98-99 0.11358623706231086 1107 100 0.13324441101627116 1108 1 -0.046889599014246386 1108 2 -0.03674463720883736 1108 3 0.01927836828868834 1108 4 -0.024526713720490534 1108 5 -0.019666520864916492 1108 6 -0.030136320985107545 1108 7 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2214 82-83 -0.09548776084205457 2214 84-85 -0.11950166635795867 2214 86-87 -0.10428160377087181 2214 88-89 -0.06591198842532364 2214 90-91 -0.051005458721601116 2214 92-93 -0.05056569551169332 2214 94-95 -0.0022129494851235165 2214 96-97 -0.06379662262427743 2214 98-99 -0.006412027517463059 2214 100 -0.021039593495821407 2215 1 -0.074990011924843 2215 2 -0.09359541922400538 2215 3 -0.12708186299064295 2215 4 -0.10089461339291006 2215 5 -0.004700593567569911 2215 6 -0.030790230200167912 2215 7 -0.06678885551527003 2215 8 -0.09362671376901943 2215 9 -0.08654741998500981 2215 10-11 -0.08870790703608122 2215 12-13 -0.1284681285347702 2215 14-15 -0.12481925846845598 2215 16-17 -0.05115107951035469 2215 18-19 -0.10672334124045335 2215 20-21 -0.10097677334220378 2215 22-23 -0.04890781238723463 2215 24-25 -0.10600429499390174 2215 26-27 -0.0831645436816899 2215 28-29 -0.07131890582290623 2215 30-31 -0.1228579700611121 2215 32-33 -0.1580189063260491 2215 34-35 -0.17418374910242562 2215 36-37 -0.1359572341722597 2215 38-39 -0.11992386516192255 2215 40-41 -0.19953920017916005 2215 42-43 -0.1154935361258893 2215 44-45 -0.058510087306487435 2215 46-47 -0.04304198018827776 2215 48-49 -0.017230124915862177 2215 50-51 -0.006343191644134549 2215 52-53 0.004437403270436846 2215 54-55 0.01902748890577044 2215 56-57 0.023809224029804454 2215 58-59 0.046564408057804485 2215 60-61 0.033315313662775736 2215 62-63 -0.047500559425245115 2215 64-65 0.1614921015748294 2215 66-67 0.10450838705448717 2215 68-69 0.017487652481733562 2215 70-71 -0.008931999038324534 2215 72-73 -0.01706120230653596 2215 74-75 -0.008589520298045272 2215 76-77 0.031946315954449744 2215 78-79 0.02501264748982379 2215 80-81 0.013982060022136977 2215 82-83 0.06477005415701598 2215 84-85 0.10600444460661507 2215 86-87 0.06002735915160429 2215 88-89 -0.06648853044028513 2215 90-91 -0.04596805897930878 2215 92-93 0.029204818899131624 2215 94-95 0.005209068647971549 2215 96-97 0.03955327108476325 2215 98-99 0.08775157293186098 2215 100 0.05500930669088433 2216 1 0.034213749821152106 2216 2 -0.12590277714326703 2216 3 -0.18609791196107395 2216 4 -0.10510520131863643 2216 5 -0.040212736626557444 2216 6 -0.10633481489993812 2216 7 0.017634498860758185 2216 8 -0.10043876570600929 2216 9 -0.10927996196755885 2216 10-11 -0.08610090428641115 2216 12-13 -0.06461664626171881 2216 14-15 -0.04883823723655922 2216 16-17 -0.050357136335243524 2216 18-19 -0.20933109624942148 2216 20-21 -0.1751830818758009 2216 22-23 -0.1495902308283874 2216 24-25 -0.20222988673126707 2216 26-27 -0.19734552125961358 2216 28-29 -0.10823945946648905 2216 30-31 -0.15880403829878276 2216 32-33 -0.1968002123247885 2216 34-35 -0.18251894263386248 2216 36-37 -0.20123183966214953 2216 38-39 -0.16362083016491624 2216 40-41 -0.15709339375295173 2216 42-43 -0.13547299256672574 2216 44-45 -0.1127605507085434 2216 46-47 -0.04155843541313686 2216 48-49 -0.11739217716361594 2216 50-51 -0.08858823932968818 2216 52-53 -0.08815952356228252 2216 54-55 -0.17345939248338027 2216 56-57 -0.011737229392146276 2216 58-59 0.00523790841915428 2216 60-61 0.03397241674387885 2216 62-63 0.055373399048257 2216 64-65 -0.039908272678758294 2216 66-67 -0.08820264696329616 2216 68-69 -0.17380350358415342 2216 70-71 -0.21049530164653163 2216 72-73 -0.17897711453319687 2216 74-75 -0.13265711152960336 2216 76-77 -0.12024825031612352 2216 78-79 -0.05895425979814206 2216 80-81 -0.07797585923506745 2216 82-83 -0.067985501226282 2216 84-85 0.016140592759679606 2216 86-87 -0.049791959403762576 2216 88-89 -0.023509697847178046 2216 90-91 -0.01971822316333771 2216 92-93 -0.03919467858248993 2216 94-95 -0.07937560865125093 2216 96-97 -0.11074200100914311 2216 98-99 -0.18861676092411628 2216 100 -0.18487950846792955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74507.0 3 15902.0 4 11610.0 5 10620.0 6 7509.0 7 6455.0 8 6906.0 9 7368.0 10 7347.0 11 8006.0 12 8794.0 13 9837.0 14 10210.0 15 10242.0 16 10331.0 17 10352.0 18 10131.0 19 10154.0 20 9900.0 21 10478.0 22 12420.0 23 19221.0 24 30715.0 25 39790.0 26 39561.0 27 31366.0 28 32183.0 29 38336.0 30 46378.0 31 56019.0 32 72391.0 33 98329.0 34 143153.0 35 230349.0 36 404782.0 37 759117.0 38 1118656.0 39 505238.0 40 1578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.58617521916877 21.79201145459774 14.095877381335875 22.52593594489761 2 15.087262187452444 22.404979776390725 39.769287500435055 22.73847053572177 3 17.391313184329924 28.816096372147946 30.252568376783838 23.540022066738292 4 12.042021817261697 19.356487572788353 39.292106352227925 29.309384257722026 5 12.358338831387611 40.28574469906695 34.93309987879299 12.422816590752445 6 27.999149442323272 40.44805691521429 18.702284743235996 12.850508899226446 7 24.96488400989675 34.90406202262514 23.690393957077323 16.440660010400787 8 23.814116056410164 37.5503430811274 20.973766596100187 17.661774266362247 9 24.89717982206882 18.60635565759312 21.483669318011778 35.01279520232628 10-11 22.691090306716486 29.155925666136806 29.14049215990586 19.01249186724085 12-13 23.31230481060484 27.383066738037638 28.546905537542035 20.757722913815492 14-15 21.29449136879576 28.64466377947895 26.101603026847187 23.9592418248781 16-17 20.89988900577988 31.519525862364627 26.62794275045659 20.952642381398903 18-19 20.96389677359694 30.694131787154294 28.245272583665482 20.096698855583284 20-21 22.368129898550418 29.52422120495163 27.761041562241743 20.346607334256213 22-23 22.025340928058014 29.565936130435105 27.59507103350633 20.81365190800055 24-25 21.548401126861897 29.805441282685692 27.61028859767479 21.035868992777626 26-27 21.34753690132286 29.743135646420022 27.71199730910785 21.197330143149262 28-29 21.45243393379623 29.55675224154212 28.021569309399503 20.96924451526215 30-31 21.651824672320622 29.640906133542128 27.69027607811615 21.016993116021094 32-33 21.249854874231534 29.691220634102432 27.846211652182884 21.212712839483153 34-35 21.740945231757912 29.39045144847584 27.79143858315586 21.077164736610385 36-37 21.759440034286516 29.451436027418037 27.52317502917123 21.265948909124212 38-39 21.59460002576062 29.52444984948838 27.699523479380456 21.181426645370546 40-41 21.47324058664091 29.425738921981658 27.85759306912356 21.243427422253873 42-43 21.459344079795926 29.51574865461744 27.809996898055783 21.21491036753085 44-45 21.407136910570262 29.455145149903167 27.774785639395557 21.362932300131014 46-47 21.601599089080164 29.304239755645046 27.757129200168386 21.337031955106408 48-49 21.540042898796084 29.25001289301138 27.793877458214578 21.41606674997796 50-51 21.4884454483351 29.320829187034025 27.705900121461056 21.484825243169816 52-53 21.462583210733285 29.391226299405954 27.68962825192868 21.45656223793208 54-55 21.45103666162717 29.56761285703797 27.659625007716755 21.321725473618105 56-57 21.526527466179026 29.355913420951612 27.60356898878905 21.51399012408031 58-59 21.6873026829404 28.99018378193815 27.706776592185285 21.615736942936167 60-61 21.708299872899044 29.21859967415613 27.69039040038453 21.3827100525603 62-63 21.374011572717674 29.196691614752705 27.80168030891405 21.627616503615567 64-65 21.575005589090097 29.17083307928378 27.711229498201327 21.54293183342479 66-67 21.492522942573892 29.064709198445925 27.698240529479772 21.744527329500404 68-69 21.693336358215873 29.250863958787075 27.563861054239315 21.491938628757744 70-71 21.69794735637376 29.063210306482755 27.712949994677665 21.52589234246582 72-73 21.547689788303103 29.054648838828722 27.750498508602494 21.647162864265677 74-75 21.52586693751729 29.16089233357409 27.790536707483106 21.52270402142552 76-77 21.608623557348242 29.091054130069782 27.61938356924792 21.680938743334057 78-79 21.56713727640152 29.164957125338614 27.66619218691132 21.601713411348545 80-81 21.462392673619323 29.226437100777115 27.804763478658955 21.50640674694461 82-83 21.461429141951854 29.17831908452644 27.81230596945915 21.547945804062564 84-85 21.502244413862346 28.99828693387585 27.737634891572288 21.761833760689512 86-87 21.697212879089182 29.07466843927555 27.59661647817454 21.631502203460727 88-89 21.445326473122826 29.069392560404538 27.763591529543756 21.72168943692888 90-91 21.42046525618662 29.053779399891365 27.70510249344919 21.820652850472822 92-93 21.57567121291436 29.086513269920694 27.694578736497206 21.64323678066774 94-95 21.504164261093738 28.84944701991592 27.860338946019258 21.78604977297108 96-97 21.549051865192496 29.11923007498014 27.605171568188464 21.726546491638903 98-99 21.251706770669852 29.139226121692413 27.700032125206242 21.90903498243149 100 21.257091320198672 29.01650089554514 27.69866493909023 22.027742845165964 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18090.0 1 12402.5 2 5346.5 3 3261.0 4 2133.5 5 1625.5 6 1493.0 7 1563.5 8 1782.0 9 1843.5 10 1510.5 11 1237.0 12 1187.5 13 1158.5 14 1235.0 15 1415.5 16 1685.5 17 2124.0 18 2730.0 19 3632.0 20 5150.5 21 7017.0 22 9436.5 23 13402.0 24 18737.0 25 24909.5 26 32615.0 27 41956.5 28 53276.5 29 65203.5 30 74994.5 31 84040.0 32 92298.0 33 98639.0 34 104736.5 35 112478.0 36 119741.0 37 125149.5 38 130902.5 39 134481.0 40 139005.5 41 143405.0 42 144372.5 43 147000.0 44 146381.5 45 141892.0 46 137736.0 47 131034.0 48 123013.5 49 119242.5 50 118162.0 51 113097.0 52 103651.0 53 95030.0 54 89380.5 55 85097.5 56 77501.0 57 68034.0 58 61889.5 59 57404.5 60 51496.5 61 43498.0 62 36603.5 63 30973.5 64 25373.0 65 20803.5 66 17013.0 67 13430.0 68 10535.0 69 8272.0 70 6339.0 71 4920.0 72 3884.5 73 3093.0 74 2500.0 75 1927.5 76 1369.0 77 1016.0 78 800.5 79 584.5 80 419.0 81 277.0 82 193.5 83 156.5 84 127.0 85 108.0 86 91.5 87 73.5 88 54.0 89 46.5 90 48.5 91 36.0 92 28.5 93 23.0 94 21.0 95 23.0 96 21.5 97 19.0 98 17.0 99 29.0 100 55.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.270247426415202E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.2702474264152018E-5 64-65 2.5404948528304037E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.270247426415202E-4 84-85 6.097187646792967E-4 86-87 0.0011813301065661376 88-89 0.002870759183698356 90-91 0.0022102305219624512 92-93 0.0016386191800756103 94-95 0.0017402389741888263 96-97 0.0016640241286039143 98-99 0.0020196934080001705 100 0.0023118503160756672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3936241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.197843609016175 #Duplication Level Percentage of deduplicated Percentage of total 1 73.37274094344107 39.76644338817477 2 15.016505913966997 16.277244781580993 3 5.1615789324932155 8.392393432765799 4 2.279837686984404 4.942491456524876 5 1.201738324645867 3.256581288905891 6 0.6916885815930639 2.249281774279386 7 0.4568901107379777 1.7333721137798095 8 0.31827253953539786 1.3799748258227127 9 0.23573042059317906 1.149847242227705 >10 1.1262069050310286 10.974132765877478 >50 0.08608588198161488 3.2090928766081097 >100 0.049720511654552574 4.908461003842458 >500 0.0024870642280915727 0.8955630382802658 >1k 4.6925737584360515E-4 0.29415504618532323 >5k 0.0 0.0 >10k+ 4.6925737584358094E-5 0.5709649651444063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22450 0.5703410944604256 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 1.2702474264152017E-5 0.0 2.5404948528304034E-5 0.0 18-19 0.0 2.5404948528304034E-5 0.0 2.5404948528304034E-5 0.0 20-21 0.0 2.5404948528304034E-5 0.0 3.8107422792456054E-5 0.0 22-23 0.0 2.5404948528304034E-5 0.0 1.7783463969812824E-4 0.0 24-25 0.0 2.5404948528304034E-5 0.0 5.335039190943846E-4 0.0 26-27 0.0 2.5404948528304034E-5 0.0 0.0010924127867170735 0.0 28-29 0.0 2.5404948528304034E-5 0.0 0.0024388750587171874 0.0 30-31 0.0 2.5404948528304034E-5 0.0 0.00585584063577408 0.0 32-33 0.0 2.5404948528304034E-5 0.0 0.012524639624453889 0.0 34-35 0.0 2.5404948528304034E-5 0.0 0.02280094130415287 0.0 36-37 0.0 2.5404948528304034E-5 0.0 0.038577414340229675 0.0 38-39 0.0 2.5404948528304034E-5 0.0 0.05883786079155215 0.0 40-41 0.0 2.5404948528304034E-5 0.0 0.07922533198551612 0.0 42-43 0.0 2.5404948528304034E-5 0.0 0.09909200173464988 0.0 44-45 0.0 2.5404948528304034E-5 0.0 0.12637691645404842 0.0 46-47 0.0 2.5404948528304034E-5 0.0 0.16113088604076836 0.0 48-49 0.0 2.5404948528304034E-5 0.0 0.19678673130024305 0.0 50-51 0.0 2.5404948528304034E-5 0.0 0.23194718006341583 0.0 52-53 0.0 2.5404948528304034E-5 0.0 0.2670441164552679 0.0 54-55 0.0 2.5404948528304034E-5 0.0 0.3071331252329316 0.0 56-57 0.0 2.5404948528304034E-5 0.0 0.3568506095028226 0.0 58-59 0.0 2.5404948528304034E-5 0.0 0.41603143709950685 0.0 60-61 0.0 2.5404948528304034E-5 0.0 0.4779941065600404 0.0 62-63 0.0 2.5404948528304034E-5 0.0 0.5416716100462344 0.0 64-65 0.0 2.5404948528304034E-5 0.0 0.6113319789108441 0.0 66-67 0.0 2.5404948528304034E-5 0.0 0.6945458878153039 0.0 68-69 0.0 2.5404948528304034E-5 0.0 0.7895349903626327 0.0 70-71 0.0 2.5404948528304034E-5 0.0 0.8957022702624153 0.0 72-73 0.0 2.5404948528304034E-5 0.0 1.00722999430167 0.0 74-75 0.0 2.5404948528304034E-5 0.0 1.1245881540281704 0.0 76-77 0.0 2.5404948528304034E-5 0.0 1.2511937150189736 0.0 78-79 0.0 2.5404948528304034E-5 0.0 1.399126730299288 0.0 80-81 0.0 2.5404948528304034E-5 0.0 1.5722233470968876 0.0 82-83 0.0 2.5404948528304034E-5 0.0 1.7540211587654313 0.0 84-85 2.5404948528304034E-5 2.5404948528304034E-5 0.0 1.9385372999265034 0.0 86-87 2.5404948528304034E-5 2.5404948528304034E-5 0.0 2.138359922575879 0.0 88 2.5404948528304034E-5 2.5404948528304034E-5 0.0 2.3037461374951382 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4265 0.0 46.834396 1 GTATCAA 7870 0.0 38.161125 1 TCAACGC 8915 0.0 32.580833 4 ATCAACG 9010 0.0 32.498123 3 CAACGCA 9820 0.0 29.673944 5 AACGCAG 10305 0.0 28.186134 6 TATCAAC 10875 0.0 27.097757 2 GTACATG 18655 0.0 25.017796 1 ACGCAGA 11515 0.0 24.856983 7 TACATGG 18210 0.0 24.519337 2 CGCAGAG 11670 0.0 24.446287 8 ACATGGG 18815 0.0 22.931555 3 GCAGAGT 13175 0.0 20.440853 9 GTGGTAT 2345 0.0 19.441238 1 CATGGGT 3565 0.0 19.116285 4 CATGGGG 10860 0.0 18.998957 4 TGGTATC 2235 0.0 18.295185 2 CATGGGA 5800 0.0 17.665403 4 CAGAGTA 12760 0.0 17.661718 10-11 AGAGTAC 12050 0.0 17.376236 10-11 >>END_MODULE