FastQCFastQC Report
Wed 25 May 2016
SRR2431344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936241
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232800.5914272017389179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166590.4232210375330169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138820.3526714954699166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80950.058.644541
GTATCAA167300.046.3284531
ATCAACG216950.035.2457583
TCAACGC217400.035.1728024
CAACGCA224900.033.9998555
TATCAAC229650.033.521732
AACGCAG233300.032.8159756
ACGCAGA261250.029.1792077
CGCAGAG264100.028.8109388
GTGGTAT29200.028.0084761
GCAGAGT285850.026.4050089
TGGTATC31650.025.3922622
GTACATG176850.025.0628071
TACATGG172500.024.820372
ACATGGG173900.023.8097743
AGAGTAC277500.021.85616710-11
GAGTACT189250.021.74198512-13
AGTACTT192000.021.3938612-13
CAGAGTA282800.021.29698610-11
GTACTTT200550.020.35289214-15
Produced by FastQC (version 0.11.5)