##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431344_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3936241
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.744894431006635	34.0	31.0	34.0	31.0	34.0
2	32.88758538920762	34.0	31.0	34.0	31.0	34.0
3	32.95769034467148	34.0	31.0	34.0	31.0	34.0
4	36.34821089460732	37.0	37.0	37.0	35.0	37.0
5	36.30349488255419	37.0	37.0	37.0	35.0	37.0
6	36.33729743681853	37.0	37.0	37.0	35.0	37.0
7	36.207993108145565	37.0	37.0	37.0	35.0	37.0
8	36.26419164883451	37.0	37.0	37.0	35.0	37.0
9	38.0800680141282	39.0	39.0	39.0	35.0	39.0
10-11	38.06892705502534	39.0	39.0	39.0	35.0	39.0
12-13	38.0501342524505	39.0	39.0	39.0	35.0	39.0
14-15	39.53521913419427	41.0	39.5	41.0	37.0	41.0
16-17	39.545428112760376	41.0	39.5	41.0	37.0	41.0
18-19	39.61726022364992	41.0	40.0	41.0	37.0	41.0
20-21	39.621259470647246	41.0	40.0	41.0	37.0	41.0
22-23	39.55603734629054	41.0	40.0	41.0	37.0	41.0
24-25	39.52865881433581	41.0	40.0	41.0	36.0	41.0
26-27	39.441823048944414	41.0	39.5	41.0	36.0	41.0
28-29	39.35970079067822	41.0	39.0	41.0	36.0	41.0
30-31	39.201247840261814	41.0	39.0	41.0	35.5	41.0
32-33	39.017420554280086	41.0	39.0	41.0	35.0	41.0
34-35	38.927379192483386	41.0	39.0	41.0	35.0	41.0
36-37	38.829663376810515	41.0	38.5	41.0	35.0	41.0
38-39	38.71754676606437	41.0	38.0	41.0	35.0	41.0
40-41	38.626158941995676	41.0	38.0	41.0	35.0	41.0
42-43	38.49868427771572	41.0	38.0	41.0	35.0	41.0
44-45	38.307721376816104	41.0	38.0	41.0	34.5	41.0
46-47	38.02725379365745	40.0	38.0	41.0	34.0	41.0
48-49	37.77762540454205	40.0	38.0	41.0	33.0	41.0
50-51	37.62520917799495	40.0	38.0	41.0	33.0	41.0
52-53	37.51166277674563	40.0	37.0	41.0	33.0	41.0
54-55	37.36027316416856	40.0	37.0	41.0	33.0	41.0
56-57	37.18987328265723	40.0	36.5	41.0	33.0	41.0
58-59	37.00055522515009	40.0	36.0	41.0	32.5	41.0
60-61	36.8002157388229	39.5	35.5	41.0	32.0	41.0
62-63	36.57048806208766	39.0	35.0	41.0	32.0	41.0
64-65	36.3000146586553	39.0	35.0	41.0	31.5	41.0
66-67	36.009380142120364	38.5	35.0	41.0	31.0	41.0
68-69	35.735668623948584	37.5	35.0	40.0	31.0	41.0
70-71	35.476041609240895	37.0	35.0	40.0	31.0	41.0
72-73	35.21037024917936	36.5	35.0	39.0	31.0	41.0
74-75	34.90641071011658	36.0	35.0	39.0	31.0	41.0
76-77	34.58212962570127	36.0	35.0	39.0	31.0	40.5
78-79	34.26234000916102	35.0	35.0	37.5	30.5	39.5
80-81	33.9528583234614	35.0	35.0	37.0	30.0	39.0
82-83	33.63675407577941	35.0	35.0	36.5	30.0	39.0
84-85	33.37690552992055	35.0	34.0	36.0	30.0	37.5
86-87	33.15720328099829	35.0	34.0	36.0	30.0	37.0
88-89	32.93638461161296	35.0	34.0	35.5	29.5	37.0
90-91	32.74508331679894	35.0	34.0	35.0	29.0	36.0
92-93	32.60436111508416	35.0	34.0	35.0	29.0	36.0
94-95	32.47327170770286	35.0	34.0	35.0	29.0	36.0
96-97	32.34211472315846	35.0	34.0	35.0	29.0	36.0
98-99	32.228224719980304	35.0	34.0	35.0	29.0	36.0
100	32.12516103561748	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27143164261663344
1101	2	0.2530674261759458
1101	3	0.2459250708431071
1101	4	0.179550735651965
1101	5	0.20890292816432776
1101	6	0.22862095238836133
1101	7	0.238098509658478
1101	8	0.23192582122454297
1101	9	0.31771263527555504
1101	10-11	0.3255269192415966
1101	12-13	0.3170642919794915
1101	14-15	0.39626258279598403
1101	16-17	0.4199050828608861
1101	18-19	0.40132803867078337
1101	20-21	0.4059715222863147
1101	22-23	0.39804958083612973
1101	24-25	0.3952691532461756
1101	26-27	0.4473670536217327
1101	28-29	0.4457734182661781
1101	30-31	0.48701902188371804
1101	32-33	0.4471351288958161
1101	34-35	0.4768666492660074
1101	36-37	0.4583977996484876
1101	38-39	0.45973739417792814
1101	40-41	0.4777910052058445
1101	42-43	0.5317700887703012
1101	44-45	0.5721573528214492
1101	46-47	0.6122732249035394
1101	48-49	0.5587763707565685
1101	50-51	0.614131387632888
1101	52-53	0.6156652511477958
1101	54-55	0.5739253627141281
1101	56-57	0.5623117678665821
1101	58-59	0.607201769947693
1101	60-61	0.6257921068650703
1101	62-63	0.6162697865074733
1101	64-65	0.6383092968272308
1101	66-67	0.6408166541659881
1101	68-69	0.6231024340138447
1101	70-71	0.6108090664439132
1101	72-73	0.6248390067831053
1101	74-75	0.5929811278490291
1101	76-77	0.6074970332273324
1101	78-79	0.5776596830687595
1101	80-81	0.6247663357687827
1101	82-83	0.6898629403358925
1101	84-85	0.6173376868728297
1101	86-87	0.6189767466562728
1101	88-89	0.6526913306264106
1101	90-91	0.6200813706846446
1101	92-93	0.669921835770964
1101	94-95	0.6649468245339065
1101	96-97	0.6889145181333802
1101	98-99	0.7156802595247029
1101	100	0.7513409602535717
1102	1	0.12084764330100484
1102	2	0.16201315886992518
1102	3	0.14402489396538698
1102	4	0.1239660031745018
1102	5	0.1419812682859387
1102	6	0.12374076060927308
1102	7	0.16631530088932323
1102	8	0.16678626875481228
1102	9	0.24009939865659646
1102	10-11	0.2503913554828543
1102	12-13	0.2655405351417741
1102	14-15	0.31269785806557593
1102	16-17	0.3570595730662163
1102	18-19	0.3375021594945551
1102	20-21	0.3121670406938506
1102	22-23	0.30865782108336504
1102	24-25	0.33446394289214254
1102	26-27	0.3067100202572419
1102	28-29	0.29857129777837343
1102	30-31	0.27424887310169055
1102	32-33	0.31527204172410705
1102	34-35	0.34130894088789177
1102	36-37	0.25128168428544484
1102	38-39	0.2506809721655756
1102	40-41	0.30925005743789313
1102	42-43	0.3016158025302005
1102	44-45	0.33155486333258466
1102	46-47	0.29061620021015244
1102	48-49	0.2598298104244705
1102	50-51	0.30663985199598187
1102	52-53	0.27948422902572645
1102	54-55	0.27512211837270684
1102	56-57	0.24649701734933416
1102	58-59	0.2653107242673798
1102	60-61	0.21858737469045053
1102	62-63	0.2463338284849641
1102	64-65	0.26553220727393523
1102	66-67	0.28606222584908636
1102	68-69	0.26257888592988365
1102	70-71	0.2787420334508539
1102	72-73	0.26718325066030246
1102	74-75	0.2059746159742062
1102	76-77	0.19363413358456683
1102	78-79	0.21032653044827754
1102	80-81	0.12599444655243985
1102	82-83	0.1843202886299551
1102	84-85	0.17305266396492414
1102	86-87	0.1440315008386932
1102	88-89	0.17082056887450392
1102	90-91	0.1847048788167882
1102	92-93	0.21479263427803374
1102	94-95	0.178734311396191
1102	96-97	0.17263811508617266
1102	98-99	0.19077220241909743
1102	100	0.18112939032565833
1103	1	0.18518468914681563
1103	2	0.2009931287490545
1103	3	0.1710728692734591
1103	4	0.10637659627851548
1103	5	0.1605599943322744
1103	6	0.1604437057323338
1103	7	0.15450389241802043
1103	8	0.17244021743644566
1103	9	0.20814703273166657
1103	10-11	0.23904317723093982
1103	12-13	0.2628816757836532
1103	14-15	0.3032847660070672
1103	16-17	0.3305151730077185
1103	18-19	0.2930205129555077
1103	20-21	0.27822898089853965
1103	22-23	0.34021289429451684
1103	24-25	0.3105502440546388
1103	26-27	0.30476948203273935
1103	28-29	0.31871969565093394
1103	30-31	0.3386711630573842
1103	32-33	0.322670576218151
1103	34-35	0.322452930953645
1103	36-37	0.3455075343533025
1103	38-39	0.3425986246877457
1103	40-41	0.27342694185227145
1103	42-43	0.33136920395619995
1103	44-45	0.3085676249338576
1103	46-47	0.24512849357817146
1103	48-49	0.23830961738408263
1103	50-51	0.2686670967575395
1103	52-53	0.254563892821416
1103	54-55	0.2563314686701972
1103	56-57	0.2587043715620183
1103	58-59	0.2640106393439723
1103	60-61	0.21925861593192053
1103	62-63	0.230852240956132
1103	64-65	0.24377732032982635
1103	66-67	0.2566673756452431
1103	68-69	0.20382407852127926
1103	70-71	0.2557488940302335
1103	72-73	0.21914687984008197
1103	74-75	0.19890602557439507
1103	76-77	0.1935134192017216
1103	78-79	0.14265457338719045
1103	80-81	0.19628671390465513
1103	82-83	0.24062649265650293
1103	84-85	0.21141090987332944
1103	86-87	0.22405218007197192
1103	88-89	0.26507579576879436
1103	90-91	0.26160244220787376
1103	92-93	0.29153305089017323
1103	94-95	0.29242019230331096
1103	96-97	0.29903312949236494
1103	98-99	0.3294005483626563
1103	100	0.36959609958231
1104	1	0.10035655598461801
1104	2	0.13581932830574317
1104	3	0.1659281895429885
1104	4	0.04041940937374733
1104	5	0.1001720516824065
1104	6	0.12625808205861944
1104	7	0.06269786803985511
1104	8	0.1312748917098574
1104	9	0.16493337423340648
1104	10-11	0.13333431438127263
1104	12-13	0.17655794479667009
1104	14-15	0.25278367060419527
1104	16-17	0.25719349655333446
1104	18-19	0.2287528982696827
1104	20-21	0.23599748657751007
1104	22-23	0.22507320817701526
1104	24-25	0.23709171129905826
1104	26-27	0.26489804531473027
1104	28-29	0.25270635745595627
1104	30-31	0.24671695697431772
1104	32-33	0.2575836652542378
1104	34-35	0.2684978943409746
1104	36-37	0.2961557832740098
1104	38-39	0.3116711723388548
1104	40-41	0.31110481054020056
1104	42-43	0.2839432779279605
1104	44-45	0.28101038560690483
1104	46-47	0.3301707505316287
1104	48-49	0.2667499159327704
1104	50-51	0.1819905441490448
1104	52-53	0.23089766628019248
1104	54-55	0.29515568919698865
1104	56-57	0.2655097728895086
1104	58-59	0.21411130959916136
1104	60-61	0.268646737210112
1104	62-63	0.24368358574500348
1104	64-65	0.2835887999308966
1104	66-67	0.28756803374634643
1104	68-69	0.24585530655721044
1104	70-71	0.2605931896791276
1104	72-73	0.2428379056392913
1104	74-75	0.2752207698995761
1104	76-77	0.2547762416150974
1104	78-79	0.23977270317938348
1104	80-81	0.23869688258257327
1104	82-83	0.2493390582072621
1104	84-85	0.24611114884241658
1104	86-87	0.26795334275202976
1104	88-89	0.2709022249027697
1104	90-91	0.27605850670067866
1104	92-93	0.3001541538310377
1104	94-95	0.24717130658185482
1104	96-97	0.2557146419524585
1104	98-99	0.2630001361056813
1104	100	0.24878430446592148
1105	1	-0.027422115339497566
1105	2	0.025339480508733914
1105	3	0.03411301532172217
1105	4	-0.07338638148318921
1105	5	-0.10454275804205793
1105	6	-0.11878182898378498
1105	7	-0.444110543625591
1105	8	-0.37116576992976746
1105	9	-0.3273476502467858
1105	10-11	-0.37870172437976635
1105	12-13	-0.2414573850449102
1105	14-15	-0.20741745016808721
1105	16-17	-0.18184525158854825
1105	18-19	-0.18504555321399607
1105	20-21	-0.14664223178805713
1105	22-23	-0.09673897025107436
1105	24-25	-0.0836466723216347
1105	26-27	-0.08480372102065559
1105	28-29	-0.15614194888460275
1105	30-31	-0.14431465502910612
1105	32-33	-0.09953260538344466
1105	34-35	-0.1404224036924404
1105	36-37	-0.14007326198478154
1105	38-39	-0.14193594217162087
1105	40-41	-0.13121571640972718
1105	42-43	-0.13445474780501598
1105	44-45	-0.09098846069726108
1105	46-47	-0.09902244783220482
1105	48-49	-0.11258825791229299
1105	50-51	-0.06869323215721579
1105	52-53	-0.10153953506438285
1105	54-55	-0.07875742902139393
1105	56-57	-0.07393651467840812
1105	58-59	-0.07757972131791746
1105	60-61	-0.11002117020077407
1105	62-63	-0.08435212518256208
1105	64-65	-0.09144500522842947
1105	66-67	-0.013207635597332512
1105	68-69	-0.10306876966852485
1105	70-71	-0.11528151929476138
1105	72-73	-0.06603148006400517
1105	74-75	-0.07024836037037119
1105	76-77	-0.04110778726557385
1105	78-79	-0.07381221460362752
1105	80-81	-0.08086301043691435
1105	82-83	-0.08920140501020768
1105	84-85	-0.14401241383444585
1105	86-87	-0.11068413125270382
1105	88-89	-0.0339593997180998
1105	90-91	-0.016381624906969705
1105	92-93	-0.031960730768439305
1105	94-95	-0.07385475425856924
1105	96-97	-0.029373132288419868
1105	98-99	-0.062073750626758795
1105	100	-0.05454358011426308
1106	1	0.1042078528979502
1106	2	0.07425049781769388
1106	3	0.07909726343170576
1106	4	0.05326580614316612
1106	5	0.07844101931489433
1106	6	0.07228503671184683
1106	7	0.08919334836409831
1106	8	0.10094554028457026
1106	9	0.10264497313715992
1106	10-11	0.12991676627496673
1106	12-13	0.10327380117818308
1106	14-15	0.13325454078906773
1106	16-17	0.17388702343301787
1106	18-19	0.18859907177431978
1106	20-21	0.14583583908075326
1106	22-23	0.11007995683129934
1106	24-25	0.13344116579004606
1106	26-27	0.1776684281622991
1106	28-29	0.17198161187625516
1106	30-31	0.16397742743442478
1106	32-33	0.1354052897472755
1106	34-35	0.16717421699654977
1106	36-37	0.18312054457102533
1106	38-39	0.13737608148976932
1106	40-41	0.1112218383375847
1106	42-43	0.17156205689580162
1106	44-45	0.15341997608135216
1106	46-47	0.1526299647171001
1106	48-49	0.1604810184432992
1106	50-51	0.1696126378691858
1106	52-53	0.17805931891674476
1106	54-55	0.2619966909459137
1106	56-57	0.25617133780605883
1106	58-59	0.18177932280836728
1106	60-61	0.16526781723721484
1106	62-63	0.19874931472706692
1106	64-65	0.21420074086157115
1106	66-67	0.2066901114286921
1106	68-69	0.1986389824960355
1106	70-71	0.23184957342893853
1106	72-73	0.20466552318251274
1106	74-75	0.1926576973231846
1106	76-77	0.20575329509109963
1106	78-79	0.20868998771340586
1106	80-81	0.19817512132989634
1106	82-83	0.1928907092081502
1106	84-85	0.2119993654449317
1106	86-87	0.1666322902736468
1106	88-89	0.20999769166564874
1106	90-91	0.19699537004181877
1106	92-93	0.19746976516825043
1106	94-95	0.23853371073267482
1106	96-97	0.280671978259285
1106	98-99	0.25351920240427717
1106	100	0.2583993949335124
1107	1	-0.029182545613124944
1107	2	0.01279246857083649
1107	3	-8.706897388179868E-4
1107	4	-0.07837130347640198
1107	5	-0.10703588026219535
1107	6	-0.07583223743000644
1107	7	-0.03983203346068365
1107	8	-0.0335227785826504
1107	9	-0.04025975605380694
1107	10-11	0.0032555676144383483
1107	12-13	0.048247111345439464
1107	14-15	0.03165960840014037
1107	16-17	0.011907072365964666
1107	18-19	-0.009276652244885497
1107	20-21	0.020386672824820096
1107	22-23	1.2073851512894862E-4
1107	24-25	-0.001195544829997175
1107	26-27	0.006460728296211471
1107	28-29	0.03500449313488474
1107	30-31	0.04866407960008701
1107	32-33	0.010377236084693209
1107	34-35	0.058879822590917286
1107	36-37	0.08070778369099685
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2216	54-55	-0.4565020063444365
2216	56-57	-0.46767839037931225
2216	58-59	-0.571204148941689
2216	60-61	-0.5629220408545095
2216	62-63	-0.5790893707007427
2216	64-65	-0.5026998594802308
2216	66-67	-0.43667493083682984
2216	68-69	-0.4174118378828382
2216	70-71	-0.4115273934391581
2216	72-73	-0.4696124558954011
2216	74-75	-0.4075495555229196
2216	76-77	-0.3747287881872765
2216	78-79	-0.3810044704345259
2216	80-81	-0.4293414484246654
2216	82-83	-0.4002366705977991
2216	84-85	-0.39338825758837714
2216	86-87	-0.3535358789054541
2216	88-89	-0.32915871164698274
2216	90-91	-0.3627072736638439
2216	92-93	-0.5301201965736695
2216	94-95	-0.5128287783415502
2216	96-97	-0.5865706955113907
2216	98-99	-0.5603113495686856
2216	100	-0.5838303801209364
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57.0
3	377.0
4	199.0
5	325.0
6	406.0
7	701.0
8	1327.0
9	1957.0
10	2538.0
11	3486.0
12	5337.0
13	7701.0
14	8614.0
15	11488.0
16	15014.0
17	23388.0
18	28697.0
19	15656.0
20	9945.0
21	9064.0
22	9118.0
23	9238.0
24	10366.0
25	12429.0
26	15348.0
27	19664.0
28	24735.0
29	30554.0
30	38891.0
31	49501.0
32	62024.0
33	80606.0
34	112769.0
35	189238.0
36	335291.0
37	750186.0
38	1320444.0
39	714622.0
40	4940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.598769840695155	22.072057995184345	15.260881020490036	24.06829114363046
2	13.249471576936367	22.33159055342154	40.26969900209337	24.14923886754873
3	15.15837759315649	28.694566826193412	31.883992433361296	24.2630631472888
4	10.512595511772375	19.007515946998144	39.80982499712918	30.670063544100294
5	11.759849053957824	39.05492575276768	35.77501987302099	13.41020532025351
6	27.81219442610348	40.228177085701816	18.499146774803677	13.460481713391026
7	24.51839203951181	34.33118043331188	24.08848950051585	17.06193802666046
8	24.663454295608425	35.46012553601266	21.104906940403293	18.771513227975625
9	25.54714002521695	16.060957649696753	22.092956198566092	36.2989461265202
10-11	23.014977995503834	27.498951410749495	29.705968206723117	19.78010238702356
12-13	24.256251078122503	25.82914765635539	29.46040397424853	20.45419729127358
14-15	21.696270629770893	26.83710169169012	27.94819981804976	23.518427860489233
16-17	20.21414593262963	30.03907789182624	28.225482128761932	21.521294046782195
18-19	20.185260506152954	28.26456764207273	31.59948793785746	19.950683913916855
20-21	21.377997434608297	27.237940969569696	31.47388587233353	19.910175723488475
22-23	21.082078603458783	27.296386220450536	31.368255332056822	20.253279844033862
24-25	20.680827820983872	27.547512059422232	31.287898659570214	20.48376146002369
26-27	20.50133776532536	27.56468533267314	31.379809378444367	20.554167523557133
28-29	20.610966188778644	27.36570323519489	31.658489592013428	20.364840984013043
30-31	20.7866557982654	27.464282325395818	31.35905953523739	20.390002341101397
32-33	20.436236764012264	27.544586368753915	31.419694613686428	20.59948225354739
34-35	20.920103779011274	27.319530006686897	31.277883241498593	20.482482972803233
36-37	20.954915484396704	27.365398429793903	30.910283827359898	20.769402258449496
38-39	20.81773171366535	27.4798651933682	30.999847185954117	20.702555907012336
40-41	20.713510659838636	27.434581031261935	31.055511646086803	20.796396662812626
42-43	20.82959085591935	27.57940054054267	30.844072989138464	20.74693561439952
44-45	20.885612146557115	27.59249245438493	30.58170051952041	20.940194879537547
46-47	21.164673378005016	27.51813014068405	30.23814030296294	21.079056178347994
48-49	21.237495112623698	27.68671047754398	29.858496641778626	21.217297768053694
50-51	21.244941990481955	27.83801412908237	29.55077952572368	21.36626435471199
52-53	21.18854541389753	27.990582383188016	29.485702091335895	21.335170111578563
54-55	21.196880107605622	28.250960215566916	29.42950041861169	21.12265925821577
56-57	21.261534857925916	27.918694097714535	29.47201135302108	21.34775969133847
58-59	21.329834544266973	27.59225287007659	29.607587890816035	21.470324694840404
60-61	21.365304836386144	27.807718264752772	29.573708070492245	21.25326882836884
62-63	21.07531017536782	27.677713331068905	29.793157990072256	21.45381850349102
64-65	21.163719396246318	27.754156821190573	29.747987991588932	21.33413579097418
66-67	21.173202148203917	27.649392430831774	29.64272689638545	21.534678524578858
68-69	21.42586306480114	27.844344046362252	29.384336925316283	21.345455963520326
70-71	21.377923124655837	27.69506710507847	29.498136199396498	21.4288735708692
72-73	21.260747096688146	27.72525793984738	29.452465711228044	21.56152925223643
74-75	21.282665473967654	27.853366616105678	29.396922719684014	21.467045190242647
76-77	21.343396444994436	27.835112047089794	29.224920987103253	21.59657052081252
78-79	21.28636756262385	27.88693409887709	29.28554189319648	21.541156445302576
80-81	21.150013401198866	27.93312865277938	29.38125839162749	21.53559955439426
82-83	21.152613025137924	27.850830393953157	29.371750041410454	21.62480653949846
84-85	21.171747021498863	27.752928707019546	29.2648406791511	21.81048359233049
86-87	21.294000320864946	27.810785203273536	29.12779227767241	21.767422198189106
88-89	21.03741715654406	27.80732835745438	29.279196197133828	21.876058288867732
90-91	20.995356041674317	27.824867171664376	29.22347869684628	21.956298089815036
92-93	21.133397854983293	27.820941115885805	29.247717278815717	21.797943750315188
94-95	21.052549346388208	27.588235368838767	29.390466197771715	21.968749087001314
96-97	21.110939200580464	27.7869195224986	29.1369168295159	21.96522444740503
98-99	20.79052771162115	27.772441916992353	29.270489948885402	22.166540422501093
100	20.801404828794112	27.72949017539527	29.22867541737612	22.240429578434494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2103.0
1	1402.5
2	1733.0
3	2440.5
4	1350.5
5	912.5
6	2586.5
7	7252.5
8	12154.5
9	12178.5
10	7451.0
11	3704.0
12	3209.5
13	3425.0
14	3760.0
15	4175.5
16	4570.0
17	5131.5
18	5891.5
19	6697.0
20	7932.5
21	9800.5
22	12157.0
23	15601.0
24	20861.0
25	27091.0
26	34244.0
27	43252.5
28	54323.5
29	65952.0
30	75628.5
31	84470.0
32	92900.0
33	99088.5
34	104944.5
35	113275.5
36	120496.5
37	125174.0
38	130469.5
39	133941.0
40	138015.0
41	142248.0
42	143738.0
43	146501.5
44	146108.5
45	140953.0
46	136082.5
47	130143.0
48	121854.0
49	117583.5
50	116931.5
51	111926.0
52	102173.5
53	93779.0
54	87811.0
55	82107.0
56	74217.5
57	64983.5
58	59438.0
59	56273.0
60	50167.0
61	41028.5
62	34205.5
63	28945.0
64	23275.5
65	18787.0
66	15278.5
67	11943.0
68	9278.0
69	7182.5
70	5243.0
71	3930.0
72	3100.5
73	2344.0
74	1805.5
75	1358.0
76	905.5
77	647.5
78	486.5
79	342.5
80	200.5
81	81.0
82	41.5
83	27.5
84	11.0
85	6.5
86	6.0
87	4.0
88	3.0
89	4.0
90	5.5
91	5.0
92	3.5
93	3.5
94	4.5
95	7.5
96	10.0
97	10.5
98	5.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008332823117283724
2	2.5404948528304037E-5
3	3.8107422792456054E-4
4	0.001956181036679411
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.0809897056608074E-5
24-25	8.891731984906412E-5
26-27	3.1756185660380043E-4
28-29	8.383633014340331E-4
30-31	0.00151159443743409
32-33	0.0036710150623399333
34-35	0.004699915477736246
36-37	0.0033915606285285886
38-39	0.0021467181506416907
40-41	0.002845354235170052
42-43	2.9215690807549643E-4
44-45	3.683717536604085E-4
46-47	0.002946974029283268
48-49	0.002032395882264323
50-51	8.510657756981851E-4
52-53	2.5404948528304037E-5
54-55	3.937767021887125E-4
56-57	5.080989705660808E-4
58-59	5.589088676226888E-4
60-61	1.6513216543397621E-4
62-63	0.0
64-65	0.0
66-67	5.716113418868408E-4
68-69	5.462063933585368E-4
70-71	0.0016132142315473063
72-73	8.891731984906412E-5
74-75	0.0017910488712454345
76-77	4.1918165071701654E-4
78-79	0.0010416028896604655
80-81	6.605286617359049E-4
82-83	9.399830955472492E-4
84-85	8.383633014340331E-4
86-87	1.397272169056722E-4
88-89	0.0013210573234718097
90-91	0.0013083548492076578
92-93	0.002400767635924731
94-95	9.145781470189452E-4
96-97	0.002159420624905843
98-99	0.002172123099169995
100	0.0026167096984153156
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3936241.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.35896371582654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.57254567873184	35.539602671687675
2	16.34563268350355	16.462982464415674
3	5.7939273537804015	8.753285321434864
4	2.5460766604171163	5.128711286386335
5	1.387075810913741	3.492585021645288
6	0.790423617141278	2.388294855344999
7	0.50061072233511	1.764716604127926
8	0.36574659380216423	1.473489555717627
9	0.2546138111478647	1.1539878909429266
>10	1.2797672118863606	11.643940913841085
>50	0.09959056977323456	3.465567020608125
>100	0.059438986072628285	5.438064127904188
>500	0.0036402404236762676	1.2159378863050732
>1k	7.583833924351366E-4	0.708501424189145
>5k	0.0	0.0
>10k+	1.5167667848702682E-4	1.3703329554491646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23280	0.5914272017389179	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16659	0.4232210375330169	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13882	0.3526714954699166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.5404948528304034E-5	0.0
18-19	0.0	0.0	0.0	2.5404948528304034E-5	0.0
20-21	0.0	0.0	0.0	3.8107422792456054E-5	0.0
22-23	0.0	0.0	0.0	1.7783463969812824E-4	0.0
24-25	0.0	0.0	0.0	5.335039190943846E-4	0.0
26-27	0.0	0.0	0.0	0.0010670078381887695	0.0
28-29	0.0	0.0	0.0	0.002400767635924731	0.0
30-31	0.0	0.0	0.0	0.00585584063577408	0.0
32-33	0.0	0.0	0.0	0.012550044572982193	0.0
34-35	0.0	0.0	0.0	0.022953370995322693	0.0
36-37	0.0	0.0	0.0	0.0390474058880033	0.0
38-39	0.0	0.0	0.0	0.059460282030495595	0.0
40-41	0.0	0.0	2.5404948528304034E-5	0.07974613343034637	0.0
42-43	0.0	0.0	2.5404948528304034E-5	0.0998287452419707	0.0
44-45	0.0	0.0	2.5404948528304034E-5	0.1275201391378221	0.0
46-47	0.0	0.0	2.5404948528304034E-5	0.1627568027465798	0.0
48-49	0.0	0.0	2.5404948528304034E-5	0.1987556148111866	0.0
50-51	0.0	0.0	2.5404948528304034E-5	0.2342590303794915	0.0
52-53	0.0	0.0	2.5404948528304034E-5	0.2697497434735322	0.0
54-55	0.0	0.0	2.5404948528304034E-5	0.31024523142764887	0.0
56-57	0.0	0.0	2.5404948528304034E-5	0.3603818973482569	0.0
58-59	0.0	0.0	2.5404948528304034E-5	0.42015974123535627	0.0
60-61	0.0	0.0	2.5404948528304034E-5	0.48283374925468237	0.0
62-63	0.0	0.0	2.5404948528304034E-5	0.5469939467629141	0.0
64-65	0.0	0.0	2.5404948528304034E-5	0.6174037616091088	0.0
66-67	0.0	0.0	2.5404948528304034E-5	0.701316306598097	0.0
68-69	0.0	0.0	2.5404948528304034E-5	0.7973470120350863	0.0
70-71	0.0	0.0	2.5404948528304034E-5	0.9049115641039256	0.0
72-73	0.0	0.0	2.5404948528304034E-5	1.0184081716541238	0.0
74-75	0.0	0.0	2.5404948528304034E-5	1.1374430579834924	0.0
76-77	0.0	0.0	2.5404948528304034E-5	1.2656364282573147	0.0
78-79	0.0	0.0	2.5404948528304034E-5	1.4154240047801951	0.0
80-81	0.0	0.0	2.5404948528304034E-5	1.591226248596059	0.0
82-83	0.0	0.0	2.5404948528304034E-5	1.7760853565622634	0.0
84-85	1.2702474264152017E-5	0.0	2.5404948528304034E-5	1.9636373890724679	0.0
86-87	2.5404948528304034E-5	0.0	2.5404948528304034E-5	2.1663180684312775	0.0
88	2.5404948528304034E-5	0.0	2.5404948528304034E-5	2.3340288361408765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8095	0.0	58.64454	1
GTATCAA	16730	0.0	46.328453	1
ATCAACG	21695	0.0	35.245758	3
TCAACGC	21740	0.0	35.172802	4
CAACGCA	22490	0.0	33.999855	5
TATCAAC	22965	0.0	33.52173	2
AACGCAG	23330	0.0	32.815975	6
ACGCAGA	26125	0.0	29.179207	7
CGCAGAG	26410	0.0	28.810938	8
GTGGTAT	2920	0.0	28.008476	1
GCAGAGT	28585	0.0	26.405008	9
TGGTATC	3165	0.0	25.392262	2
GTACATG	17685	0.0	25.062807	1
TACATGG	17250	0.0	24.82037	2
ACATGGG	17390	0.0	23.809774	3
AGAGTAC	27750	0.0	21.856167	10-11
GAGTACT	18925	0.0	21.741985	12-13
AGTACTT	19200	0.0	21.39386	12-13
CAGAGTA	28280	0.0	21.296986	10-11
GTACTTT	20055	0.0	20.352892	14-15
>>END_MODULE