FastQCFastQC Report
Wed 25 May 2016
SRR2431343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957722
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95240.2406434812753397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3250.041.9381682
GGTATCA39250.038.198461
GTATCAA64250.032.8449061
TACCTGG32750.029.4196412
GTACCTG34300.029.049361
TAACGCA4900.028.775314
CAACGCA71350.027.4686245
TATCACG5400.026.9812952
AACGCAG75850.026.1487966
TCAACGC78900.025.3762474
ATCAACG81500.024.7397043
GTACATG148500.024.2436031
TACATGG142500.023.9450952
TATCAAC86400.023.3366432
ACGCAGA87650.022.6284777
ATAACGC6350.022.2045693
GTATAAC9950.022.200851
ACATGGG148450.022.162193
ACCTGGG43000.021.9696143
CGCAGAG91500.021.6249858
Produced by FastQC (version 0.11.5)