##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431343_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3957722
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.387133810813392	33.0	31.0	34.0	29.0	34.0
2	31.493300438989905	34.0	31.0	34.0	28.0	34.0
3	31.56056160589349	34.0	31.0	34.0	28.0	34.0
4	34.83990285320697	37.0	35.0	37.0	32.0	37.0
5	34.90331609951381	37.0	35.0	37.0	32.0	37.0
6	35.00205471733487	37.0	35.0	37.0	32.0	37.0
7	34.964463395862566	37.0	35.0	37.0	32.0	37.0
8	34.968535687953825	37.0	35.0	37.0	32.0	37.0
9	36.586553072701925	39.0	38.0	39.0	33.0	39.0
10-11	36.56862533548339	39.0	38.0	39.0	33.0	39.0
12-13	36.52892686752632	39.0	38.0	39.0	33.0	39.0
14-15	37.93198524302616	41.0	39.0	41.0	33.0	41.0
16-17	37.92479234266581	41.0	39.0	41.0	33.0	41.0
18-19	37.93303521571247	41.0	38.5	41.0	33.0	41.0
20-21	37.95139312463078	41.0	39.0	41.0	33.0	41.0
22-23	37.93145867749175	41.0	39.0	41.0	33.0	41.0
24-25	37.92384369089087	41.0	38.5	41.0	32.5	41.0
26-27	37.89803793697486	41.0	38.0	41.0	32.0	41.0
28-29	37.851992257162074	41.0	38.0	41.0	32.0	41.0
30-31	37.78204115903037	40.0	38.0	41.0	32.0	41.0
32-33	37.7086054806275	40.0	38.0	41.0	32.0	41.0
34-35	37.647941164134316	40.0	38.0	41.0	32.0	41.0
36-37	37.55027715438325	40.0	38.0	41.0	31.0	41.0
38-39	37.451922974883026	40.0	38.0	41.0	31.0	41.0
40-41	37.36127625942398	40.0	38.0	41.0	31.0	41.0
42-43	37.2251674069073	40.0	38.0	41.0	30.5	41.0
44-45	37.07700389264329	40.0	37.0	41.0	30.0	41.0
46-47	36.9357531933774	40.0	37.0	41.0	30.0	41.0
48-49	36.77872005663864	40.0	37.0	41.0	30.0	41.0
50-51	36.61587966512049	40.0	36.0	41.0	30.0	41.0
52-53	36.42303577664121	40.0	36.0	41.0	30.0	41.0
54-55	36.23076393440469	39.5	35.0	41.0	29.5	41.0
56-57	35.999102261351354	39.0	35.0	41.0	29.0	41.0
58-59	35.747035794833494	39.0	35.0	41.0	28.5	41.0
60-61	35.53485654121235	39.0	35.0	41.0	28.0	41.0
62-63	35.33631530966551	38.0	35.0	40.5	28.0	41.0
64-65	35.05545576470505	38.0	34.5	40.0	28.0	41.0
66-67	34.722061832539026	37.0	34.0	40.0	28.0	41.0
68-69	34.38338064169237	36.5	34.0	39.5	27.0	41.0
70-71	34.11163618869642	36.0	34.0	39.0	27.0	41.0
72-73	33.85437317224404	36.0	34.0	39.0	27.0	41.0
74-75	33.50683511878803	35.0	34.0	38.5	26.0	40.5
76-77	33.16070418791416	35.0	33.5	37.0	26.0	40.0
78-79	32.84135242445022	35.0	33.0	37.0	26.0	39.0
80-81	32.51675761966101	35.0	33.0	36.5	26.0	39.0
82-83	32.2149653260133	35.0	33.0	36.0	25.0	38.5
84-85	31.94970920140424	35.0	33.0	36.0	25.0	37.0
86-87	31.707242449065397	35.0	33.0	35.0	24.5	37.0
88-89	31.481362384725355	35.0	33.0	35.0	24.0	36.5
90-91	31.286132148746173	35.0	32.5	35.0	23.5	36.0
92-93	31.08539268296257	35.0	32.0	35.0	23.0	36.0
94-95	30.86641469512007	35.0	32.0	35.0	20.0	36.0
96-97	30.66650992666994	35.0	32.0	35.0	19.0	35.5
98-99	30.54041693681365	35.0	32.0	35.0	17.5	35.0
100	30.496491668692244	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48580868558023127
1101	2	0.523680330438804
1101	3	0.5506109217292305
1101	4	0.581580348081495
1101	5	0.4795929608763174
1101	6	0.48567488024329464
1101	7	0.5034751705132763
1101	8	0.4685966315239227
1101	9	0.5993211215692256
1101	10-11	0.5298076151480942
1101	12-13	0.5500238061761209
1101	14-15	0.6840061789899536
1101	16-17	0.6652274099130437
1101	18-19	0.650815270410618
1101	20-21	0.5966332007998574
1101	22-23	0.6399921809528593
1101	24-25	0.6284119603459715
1101	26-27	0.6900010726037493
1101	28-29	0.6809414270137708
1101	30-31	0.7314290148823872
1101	32-33	0.656290671353851
1101	34-35	0.6509997659575006
1101	36-37	0.6262985965098267
1101	38-39	0.6167974488855705
1101	40-41	0.6426651464035942
1101	42-43	0.6398855571606816
1101	44-45	0.5771647704432326
1101	46-47	0.5679999889505325
1101	48-49	0.5890823707280646
1101	50-51	0.5704569343602444
1101	52-53	0.5803720854964567
1101	54-55	0.6450054002819883
1101	56-57	0.5950234642969221
1101	58-59	0.67924142664409
1101	60-61	0.6880791211807349
1101	62-63	0.6558206670142752
1101	64-65	0.6532684093889003
1101	66-67	0.6381276376883775
1101	68-69	0.6364816910035813
1101	70-71	0.6366972451810895
1101	72-73	0.6726214091601364
1101	74-75	0.7232103153960452
1101	76-77	0.6640807945110794
1101	78-79	0.6390936174930886
1101	80-81	0.6502974810302717
1101	82-83	0.6486498992474807
1101	84-85	0.663206353359147
1101	86-87	0.6348584147319478
1101	88-89	0.7047369888454469
1101	90-91	0.6578514269650562
1101	92-93	0.6103685698646473
1101	94-95	0.6641483667807542
1101	96-97	0.6975123398300234
1101	98-99	0.732934269534713
1101	100	0.748724018473073
1102	1	0.2660597527453916
1102	2	0.3343144899771353
1102	3	0.3488239026329829
1102	4	0.30923020467071893
1102	5	0.3238710552743953
1102	6	0.3714973185373367
1102	7	0.37077207868364326
1102	8	0.4081148800327341
1102	9	0.4429969957202218
1102	10-11	0.45564676531135007
1102	12-13	0.4889886811912305
1102	14-15	0.5540913154324798
1102	16-17	0.5637121208277662
1102	18-19	0.5142594616823573
1102	20-21	0.5407672873511515
1102	22-23	0.541596083916545
1102	24-25	0.6167361450787325
1102	26-27	0.571334242050817
1102	28-29	0.5633383644818934
1102	30-31	0.586536213174675
1102	32-33	0.5817396136800923
1102	34-35	0.6034778456552274
1102	36-37	0.643072386319929
1102	38-39	0.6144991808298244
1102	40-41	0.6655937815542785
1102	42-43	0.6882764761323088
1102	44-45	0.6248118702019951
1102	46-47	0.6148828866572984
1102	48-49	0.5443398255455918
1102	50-51	0.551597146263525
1102	52-53	0.538326406112752
1102	54-55	0.5320198228907103
1102	56-57	0.5540546267755317
1102	58-59	0.5841015911027299
1102	60-61	0.541410541821385
1102	62-63	0.5687920690674204
1102	64-65	0.6189890549607711
1102	66-67	0.6537037812840367
1102	68-69	0.5725435041250648
1102	70-71	0.5430820563011451
1102	72-73	0.6365963880927268
1102	74-75	0.5879450595100906
1102	76-77	0.6136467532755674
1102	78-79	0.5434219236771014
1102	80-81	0.5550876438191565
1102	82-83	0.5851673923559204
1102	84-85	0.6181094761954036
1102	86-87	0.5703835312281065
1102	88-89	0.578221787262251
1102	90-91	0.6213821526538403
1102	92-93	0.5429875361582255
1102	94-95	0.5376238487988729
1102	96-97	0.5792002530328908
1102	98-99	0.6609849062719597
1102	100	0.6866794797048108
1103	1	0.18494912437553523
1103	2	0.17069007586565021
1103	3	0.21119500343281317
1103	4	0.30117406184020723
1103	5	0.1610010457928297
1103	6	0.2598604441444152
1103	7	0.33074600658974873
1103	8	0.3119678329352382
1103	9	0.401326576344573
1103	10-11	0.4687981212303356
1103	12-13	0.47734228864072037
1103	14-15	0.49970773456266926
1103	16-17	0.44520280797639344
1103	18-19	0.501702541642949
1103	20-21	0.48427154971698627
1103	22-23	0.5254135556756765
1103	24-25	0.5526250003767785
1103	26-27	0.5332383330423767
1103	28-29	0.5431794217543668
1103	30-31	0.506998876278594
1103	32-33	0.5049915815058057
1103	34-35	0.5392707400337642
1103	36-37	0.5682125173865558
1103	38-39	0.602625812919392
1103	40-41	0.5412198909753414
1103	42-43	0.485205025298761
1103	44-45	0.49707570485590225
1103	46-47	0.491847976196766
1103	48-49	0.517696269467038
1103	50-51	0.455083468816774
1103	52-53	0.4159905330001905
1103	54-55	0.4466111991349777
1103	56-57	0.3481692541269581
1103	58-59	0.4344548743452705
1103	60-61	0.414551188881255
1103	62-63	0.4213364938565931
1103	64-65	0.4712588627753078
1103	66-67	0.42094372634505817
1103	68-69	0.4547022242991545
1103	70-71	0.4705908328963275
1103	72-73	0.4030109235425883
1103	74-75	0.4491329980899508
1103	76-77	0.4317833264013373
1103	78-79	0.36366423245345203
1103	80-81	0.3940346570592581
1103	82-83	0.4122068651618065
1103	84-85	0.44311226997287534
1103	86-87	0.4877713109980668
1103	88-89	0.47647525786578626
1103	90-91	0.4639001861570762
1103	92-93	0.48674566123908036
1103	94-95	0.4017825605696075
1103	96-97	0.500582236654882
1103	98-99	0.5410902084719389
1103	100	0.508301141987836
1104	1	0.24347596066904842
1104	2	0.29814481915572344
1104	3	0.2696959528251597
1104	4	0.24533097522660796
1104	5	0.24529263350607522
1104	6	0.25287673708683656
1104	7	0.30609770908603195
1104	8	0.27664122122034485
1104	9	0.35922784071156144
1104	10-11	0.3456114645099646
1104	12-13	0.39420346479972324
1104	14-15	0.4096986865053296
1104	16-17	0.44985713296537
1104	18-19	0.40553306153191926
1104	20-21	0.37048941556832915
1104	22-23	0.3741537904438488
1104	24-25	0.4406518272716582
1104	26-27	0.43552796440835095
1104	28-29	0.47796817130711844
1104	30-31	0.5018810099003943
1104	32-33	0.5162611967685606
1104	34-35	0.510373478445139
1104	36-37	0.46863115345776407
1104	38-39	0.4858376436755094
1104	40-41	0.4718190602139458
1104	42-43	0.4352173053161934
1104	44-45	0.4449648036801932
1104	46-47	0.4472854970225484
1104	48-49	0.4212586843142532
1104	50-51	0.43285670229432327
1104	52-53	0.46904723292372097
1104	54-55	0.48597592352305696
1104	56-57	0.45612680299124975
1104	58-59	0.42484819979155475
1104	60-61	0.4411574922376289
1104	62-63	0.4299914670587768
1104	64-65	0.4319162203041813
1104	66-67	0.5076419828362475
1104	68-69	0.4551736683479035
1104	70-71	0.35952844943209783
1104	72-73	0.3383938197547849
1104	74-75	0.35101720350940724
1104	76-77	0.4146313681450735
1104	78-79	0.40696080638712573
1104	80-81	0.38788697332876865
1104	82-83	0.39720077165845424
1104	84-85	0.4151455384133449
1104	86-87	0.3390495027603002
1104	88-89	0.3003917105590048
1104	90-91	0.37437655001789594
1104	92-93	0.34669453395884986
1104	94-95	0.3485611406028326
1104	96-97	0.3535558253163167
1104	98-99	0.33593960622098606
1104	100	0.34281060569905364
1105	1	0.16287377660472657
1105	2	0.19743200153706653
1105	3	0.17271085710521206
1105	4	0.24616420556003504
1105	5	0.18652487379668514
1105	6	0.20618153750412205
1105	7	0.23267811625047585
1105	8	0.20406321531398675
1105	9	0.2620828036354794
1105	10-11	0.23343772155573816
1105	12-13	0.29174960875548805
1105	14-15	0.3739725391447237
1105	16-17	0.3944161728974578
1105	18-19	0.3570628141222727
1105	20-21	0.3745017961564656
1105	22-23	0.352603053081161
1105	24-25	0.37531097339081754
1105	26-27	0.3370827965029619
1105	28-29	0.3989229376372663
1105	30-31	0.3988968015343204
1105	32-33	0.44918445445721744
1105	34-35	0.4346366307551719
1105	36-37	0.43624570100834603
1105	38-39	0.47140254760575573
1105	40-41	0.4337115745697986
1105	42-43	0.4413515810403297
1105	44-45	0.4332082880619055
1105	46-47	0.3981101375528908
1105	48-49	0.4677218911947989
1105	50-51	0.45212025883038365
1105	52-53	0.5225146760153052
1105	54-55	0.49323134345559794
1105	56-57	0.4672437475244422
1105	58-59	0.43763073046415
1105	60-61	0.4429982753789119
1105	62-63	0.4256039627735859
1105	64-65	0.4842572720225675
1105	66-67	0.4844053022781978
1105	68-69	0.49819245164310644
1105	70-71	0.49492798993593823
1105	72-73	0.47233586307071107
1105	74-75	0.43766052612297557
1105	76-77	0.38220146054959514
1105	78-79	0.3803527252869472
1105	80-81	0.3872965215362143
1105	82-83	0.381216110092808
1105	84-85	0.39160170437611086
1105	86-87	0.4256626055915085
1105	88-89	0.4099058211568014
1105	90-91	0.40512741346801207
1105	92-93	0.36381826754591273
1105	94-95	0.42123213792950054
1105	96-97	0.48435574004016857
1105	98-99	0.43137965483694174
1105	100	0.44050484602231066
1106	1	0.12638008133639644
1106	2	0.15008513131168755
1106	3	0.1470171582698505
1106	4	0.07870384421954668
1106	5	0.06751784674073491
1106	6	0.13025006616226165
1106	7	0.12027765134710933
1106	8	0.1604374446861243
1106	9	0.1526739799836676
1106	10-11	0.14210066364494622
1106	12-13	0.16013521399190012
1106	14-15	0.2301272919195796
1106	16-17	0.1700242629293811
1106	18-19	0.2256081035573132
1106	20-21	0.1788708325223709
1106	22-23	0.2301044501692715
1106	24-25	0.2239197560217434
1106	26-27	0.22809393159246838
1106	28-29	0.2338891399950711
1106	30-31	0.19852896606968073
1106	32-33	0.21559236257044034
1106	34-35	0.20504605188509117
1106	36-37	0.27417897667464786
1106	38-39	0.1998959069784334
1106	40-41	0.19596574651269805
1106	42-43	0.1916293191843934
1106	44-45	0.18283709364074952
1106	46-47	0.16954304462572622
1106	48-49	0.1412255355140033
1106	50-51	0.19152047701393116
1106	52-53	0.10356730759425403
1106	54-55	0.10147997460650515
1106	56-57	0.14055911192663473
1106	58-59	0.1414239287258141
1106	60-61	0.12707099530138777
1106	62-63	0.18411624228632917
1106	64-65	0.2118491082719558
1106	66-67	0.10838031520869151
1106	68-69	0.13387509267469255
1106	70-71	0.18660507531288317
1106	72-73	0.16639348255147013
1106	74-75	0.22353477630281304
1106	76-77	0.2311695474900901
1106	78-79	0.23086896420684866
1106	80-81	0.19952738886041033
1106	82-83	0.20659488446010243
1106	84-85	0.1963361090085023
1106	86-87	0.2338824478097301
1106	88-89	0.2922165305803084
1106	90-91	0.1993815616306911
1106	92-93	0.28363471394826334
1106	94-95	0.33811683951742566
1106	96-97	0.31331272458897175
1106	98-99	0.32262043256914197
1106	100	0.3165432391926295
1107	1	0.004295910537948089
1107	2	0.02658910540493764
1107	3	0.0011954841062902233
1107	4	-0.062008539112525796
1107	5	0.03509457771252755
1107	6	-0.04380222825257363
1107	7	-0.010859114438837025
1107	8	0.006245998199823077
1107	9	0.02140362925458561
1107	10-11	-0.009336321951089133
1107	12-13	-0.0011619399771021222
1107	14-15	0.04131731133919203
1107	16-17	0.03534232107320179
1107	18-19	-0.04177764616236601
1107	20-21	0.019472226134027437
1107	22-23	0.047604221472688835
1107	24-25	0.01175368570865487
1107	26-27	0.009119916752013069
1107	28-29	0.06628684727912315
1107	30-31	0.013712302321621905
1107	32-33	0.05529774909380336
1107	34-35	0.06006932666338827
1107	36-37	0.013107199924327517
1107	38-39	0.04675876482324526
1107	40-41	0.0010833880366902804
1107	42-43	0.009983148087989946
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2216	86-87	-1.176540681102896
2216	88-89	-1.2548010572916581
2216	90-91	-1.2240553711949573
2216	92-93	-1.3271520777353878
2216	94-95	-1.3485158496406342
2216	96-97	-1.4774994952107399
2216	98-99	-1.5335715227495577
2216	100	-1.5160210119130433
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62277.0
3	11850.0
4	8135.0
5	7273.0
6	5511.0
7	5416.0
8	5832.0
9	6482.0
10	7089.0
11	7699.0
12	8570.0
13	9236.0
14	9801.0
15	9832.0
16	9339.0
17	9620.0
18	9390.0
19	9801.0
20	9722.0
21	10290.0
22	12332.0
23	17905.0
24	26818.0
25	34198.0
26	33760.0
27	28671.0
28	32095.0
29	39336.0
30	47158.0
31	57517.0
32	75078.0
33	102848.0
34	150627.0
35	241989.0
36	438824.0
37	837290.0
38	1103518.0
39	439261.0
40	15332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23945772667975	20.129928703702067	12.584578603429408	23.04603496618877
2	16.760129569550145	21.835930788433743	38.04728480033959	23.356654841676523
3	17.57258342046258	28.057705922750507	29.715225071392076	24.65448558539483
4	12.855030890267855	17.784511735168667	37.52426587132639	31.836191503237092
5	14.269799647372908	36.776130309304186	33.27899230921222	15.675077734110682
6	30.557780460578083	37.836108751448435	17.836523131235595	13.769587656737892
7	26.16386396012656	32.459505745982156	23.845964926288403	17.53066536760288
8	24.56402950990494	35.05352321360621	21.83839592573708	18.54405135075177
9	25.238786352351177	16.715373136364807	21.04872449353441	36.997116017749605
10-11	24.498650991656312	26.99079167258337	27.762447690868637	20.74810964489168
12-13	24.100012582995976	25.937888007293086	28.388464374203142	21.5736350355078
14-15	22.641850539274866	25.828203698996543	25.394519877848925	26.135425883879666
16-17	22.81551104397934	29.61808585848122	25.4763598858131	22.090043211726343
18-19	21.978716544517276	28.559926644670846	27.43420331190518	22.027153498906692
20-21	24.542880980523645	27.6427070926154	25.67162372698234	22.142788199878616
22-23	22.8887476179479	28.45331228418772	26.93130037935964	21.726639718504735
24-25	23.306588992354694	26.978233943667597	26.392619289581226	23.322557774396483
26-27	23.356087163272203	28.15006966128495	26.199705285009912	22.29413789043293
28-29	22.46379356609686	27.675503736745533	27.068715286217675	22.791987410939928
30-31	23.925606700015816	27.936942008559466	25.50656665627348	22.63088463515123
32-33	22.160765713205727	28.435751677353792	27.15515389913693	22.248328710303554
34-35	23.168251837799623	26.586278672428232	26.64617423861504	23.599295251157105
36-37	23.58687649107239	28.12371611750396	26.05745173612497	22.23195565529868
38-39	22.649582259693833	27.663122877251105	26.823005759373704	22.864289103681358
40-41	23.7028901979472	27.48154114917622	25.87003331714557	22.94553533573101
42-43	22.33384507552577	28.27629126047762	27.13303258793821	22.2568310760584
44-45	23.01135602753301	26.854058976350537	26.642598949597772	23.49198604651868
46-47	23.384083065965726	27.92723945744547	26.198037659037194	22.49063981755161
48-49	22.437035749352784	27.465787137146062	27.04078255117464	23.05639456232651
50-51	23.75248943710549	27.38325228502659	25.833054974553544	23.03120330331438
52-53	22.38202935931326	28.13399981100239	27.02371465201447	22.460256177669883
54-55	23.119764349289817	27.08939637498541	26.40938398401909	23.381455291705684
56-57	23.131349296388176	27.96377562648412	26.286510270301953	22.618364806825745
58-59	22.42131963791292	27.110153264933718	26.99304044094052	23.47548665621284
60-61	23.948915563043588	27.466204043639248	25.62409638675986	22.960784006557308
62-63	22.443579917942696	28.124650998731088	27.04794576273927	22.38382332058694
64-65	23.11181785885921	26.61739505705555	26.543552073642363	23.727235010442875
66-67	23.150779665676367	27.785655485655635	26.261180042458772	22.80238480620923
68-69	22.768868050863603	27.391855719022207	26.742365936768675	23.096910293345516
70-71	23.953501534468565	27.15682152510965	25.799828790400138	23.089848150021655
72-73	22.45589760978664	27.83368816708197	27.223071756934925	22.487342466196463
74-75	23.0366862553762	26.494875082181114	26.72509590112696	23.743342761315727
76-77	23.267778787898695	27.85204468631197	26.266296622147788	22.61387990364154
78-79	22.53211569685794	27.46905922144102	26.841324378013415	23.157500703687624
80-81	23.597693774056612	27.286002817530235	25.928403501863386	23.187899906549767
82-83	22.353487540042014	27.884174668331603	27.229125470788972	22.53321232083741
84-85	23.03604749734378	26.570150049460604	26.41739699496803	23.97640545822758
86-87	23.324321285404398	27.811765270683598	26.26699076349312	22.59692268041888
88-89	22.31556900427521	27.245495326758025	27.01718687244707	23.421748796519694
90-91	23.44686783618638	27.193664354032077	25.866524156324246	23.492943653457296
92-93	22.419437366002676	27.94036084681887	27.080339647289353	22.559862139889102
94-95	23.000485643449778	26.461592223236295	26.620462316392917	23.91745981692101
96-97	23.041600002425685	27.68282089040854	26.435778458092745	22.839800649073034
98-99	22.11283257395361	27.241955763267583	27.074102186771025	23.571109476007777
100	23.306547712298073	26.730545512983134	26.125708637263635	23.83719813745516
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7196.0
1	4909.0
2	2280.0
3	1598.5
4	1067.0
5	817.0
6	723.0
7	724.5
8	808.0
9	838.0
10	737.0
11	655.0
12	650.0
13	655.5
14	691.5
15	744.5
16	875.5
17	1094.5
18	1359.0
19	1771.5
20	2494.5
21	3363.0
22	4448.5
23	6153.0
24	8752.0
25	11791.0
26	15568.0
27	20871.5
28	27624.0
29	35277.0
30	42435.0
31	49439.0
32	56489.5
33	62836.5
34	69539.5
35	78237.0
36	87946.0
37	98987.5
38	114832.0
39	131670.0
40	146885.0
41	158292.5
42	163504.5
43	165899.5
44	163998.5
45	156865.0
46	147465.5
47	138103.0
48	129752.0
49	126767.5
50	126973.0
51	124572.0
52	120838.0
53	117298.0
54	113405.5
55	108444.0
56	102136.0
57	93927.5
58	86660.0
59	80993.5
60	74113.5
61	65056.0
62	55928.5
63	47166.5
64	38405.5
65	31251.0
66	25367.5
67	20271.5
68	16002.0
69	12593.0
70	9765.0
71	7480.0
72	6056.0
73	4992.5
74	3969.5
75	3015.0
76	2145.5
77	1522.0
78	1137.0
79	825.0
80	570.0
81	382.5
82	267.0
83	205.5
84	174.5
85	139.5
86	114.5
87	100.0
88	86.0
89	73.5
90	66.0
91	63.0
92	43.5
93	29.5
94	35.5
95	27.5
96	16.0
97	20.0
98	21.0
99	20.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.284717825051886E-4
2	5.053412038541363E-5
3	0.0
4	7.580118057812044E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.2633530096353407E-5
82-83	1.0106824077082725E-4
84-85	6.82210625203084E-4
86-87	0.0014402224309842885
88-89	0.0034742207764971874
90-91	0.0028930783920649303
92-93	0.002198234236765493
94-95	0.002172967176572786
96-97	0.0019708306950311314
98-99	0.0026783083804269225
100	0.0031836495842810586
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3957722.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.871400922902666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62705759601367	51.49763120948953
2	12.20494473545917	15.590938368807656
3	3.2026530746437	6.13673815642604
4	1.3184547291972093	3.368462024290081
5	0.7260099880700014	2.3185637511025416
6	0.4074351011348537	1.561407041678858
7	0.28111790075103293	1.2568775901831788
8	0.19906784781031445	1.0171793854681572
9	0.14081397782463162	0.8094587429867194
>10	0.8000279962479508	9.268242767128744
>50	0.059044676421981905	2.5960559845571085
>100	0.031630256462360506	3.565620174685803
>500	0.001583745434430142	0.6857367791070815
>1k	1.1878089670039726E-4	0.08623551586067772
>5k	3.959363223346389E-5	0.24085250822787635
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9524	0.2406434812753397	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	2.5267060192706814E-5	0.0	0.0	0.0
8	0.0	2.5267060192706814E-5	0.0	0.0	0.0
9	0.0	2.5267060192706814E-5	0.0	0.0	0.0
10-11	0.0	2.5267060192706814E-5	0.0	0.0	0.0
12-13	0.0	2.5267060192706814E-5	0.0	0.0	0.0
14-15	0.0	2.5267060192706814E-5	0.0	0.0	0.0
16-17	0.0	2.5267060192706814E-5	0.0	1.2633530096353407E-5	0.0
18-19	0.0	2.5267060192706814E-5	0.0	2.5267060192706814E-5	0.0
20-21	0.0	2.5267060192706814E-5	0.0	8.843471067447386E-5	0.0
22-23	0.0	2.5267060192706814E-5	0.0	3.5373884269789544E-4	0.0
24-25	2.5267060192706814E-5	2.5267060192706814E-5	0.0	6.190429747213169E-4	0.0
26-27	2.5267060192706814E-5	2.5267060192706814E-5	0.0	0.0012128188892499272	0.0
28-29	2.5267060192706814E-5	2.5267060192706814E-5	0.0	0.002766743091101396	0.0
30-31	2.5267060192706814E-5	2.5267060192706814E-5	0.0	0.005293449110372078	0.0
32-33	2.5267060192706814E-5	2.5267060192706814E-5	0.0	0.011130140014887352	0.0
34-35	2.5267060192706814E-5	2.5267060192706814E-5	0.0	0.02046631875609252	0.0
36-37	2.5267060192706814E-5	2.5267060192706814E-5	0.0	0.03459060540381563	0.0
38-39	2.5267060192706814E-5	2.5267060192706814E-5	0.0	0.05203751046687968	0.0
40-41	2.5267060192706814E-5	3.790059028906022E-5	0.0	0.07129101033372229	0.0
42-43	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.09077191374229923	0.0
44-45	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.11677171868059455	1.2633530096353407E-5
46-47	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.1479386374282984	2.5267060192706814E-5
48-49	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.1784738796711846	2.5267060192706814E-5
50-51	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.2121548709080628	2.5267060192706814E-5
52-53	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.24342285789653745	2.5267060192706814E-5
54-55	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.28113394523415236	2.5267060192706814E-5
56-57	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.3280296089518162	3.790059028906022E-5
58-59	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.37877849934886787	5.053412038541363E-5
60-61	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.43335534936511455	5.053412038541363E-5
62-63	2.5267060192706814E-5	5.053412038541363E-5	0.0	0.48935978828224924	5.053412038541363E-5
64-65	2.5267060192706814E-5	1.2633530096353409E-4	0.0	0.553159115268834	5.053412038541363E-5
66-67	2.5267060192706814E-5	1.2633530096353409E-4	0.0	0.6300973135556263	5.053412038541363E-5
68-69	2.5267060192706814E-5	1.2633530096353409E-4	0.0	0.7188604960126053	5.053412038541363E-5
70-71	2.5267060192706814E-5	1.2633530096353409E-4	0.0	0.8163155471758754	5.053412038541363E-5
72-73	2.5267060192706814E-5	1.2633530096353409E-4	0.0	0.9174216885369917	5.053412038541363E-5
74-75	2.5267060192706814E-5	1.6423589125259428E-4	0.0	1.0282303810121074	5.053412038541363E-5
76-77	2.5267060192706814E-5	2.021364815416545E-4	0.0	1.1505861199952903	5.053412038541363E-5
78-79	2.5267060192706814E-5	2.021364815416545E-4	0.0	1.2919679553035812	5.053412038541363E-5
80-81	2.5267060192706814E-5	2.021364815416545E-4	0.0	1.451289403348694	5.053412038541363E-5
82-83	2.5267060192706814E-5	2.021364815416545E-4	0.0	1.6116089002714187	6.316765048176703E-5
84-85	2.5267060192706814E-5	2.1477001163800792E-4	0.0	1.784890399073002	8.843471067447385E-5
86-87	2.5267060192706814E-5	2.2740354173436133E-4	0.0	1.976389448273527	1.0106824077082725E-4
88	2.5267060192706814E-5	2.2740354173436133E-4	0.0	2.132817817926575	1.0106824077082725E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	325	0.0	41.938168	2
GGTATCA	3925	0.0	38.19846	1
GTATCAA	6425	0.0	32.844906	1
TACCTGG	3275	0.0	29.419641	2
GTACCTG	3430	0.0	29.04936	1
TAACGCA	490	0.0	28.77531	4
CAACGCA	7135	0.0	27.468624	5
TATCACG	540	0.0	26.981295	2
AACGCAG	7585	0.0	26.148796	6
TCAACGC	7890	0.0	25.376247	4
ATCAACG	8150	0.0	24.739704	3
GTACATG	14850	0.0	24.243603	1
TACATGG	14250	0.0	23.945095	2
TATCAAC	8640	0.0	23.336643	2
ACGCAGA	8765	0.0	22.628477	7
ATAACGC	635	0.0	22.204569	3
GTATAAC	995	0.0	22.20085	1
ACATGGG	14845	0.0	22.16219	3
ACCTGGG	4300	0.0	21.969614	3
CGCAGAG	9150	0.0	21.624985	8
>>END_MODULE