FastQCFastQC Report
Wed 25 May 2016
SRR2431343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957722
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115120.29087439693844086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88010.22237539675601267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57800.14604360791384538No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.12140822422595625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.051.274991
GTATCAA103950.042.6839451
TATAACG3250.037.598342
CAACGCA138150.032.2836765
TACCTGG31050.031.4833092
TCAACGC142700.031.3539054
ATCAACG142700.031.3209693
AACGCAG144600.031.201156
TATCAAC148900.029.7642972
GTACCTG33550.029.420081
TAACGCA4750.027.7040394
ACGCAGA166650.027.0728267
CGCAGAG169850.026.53518
TACATGG138100.024.5709632
GTACATG143450.024.4758131
GTATAAC9050.023.890561
ACATGGG140250.023.4570733
GCAGAGT192800.023.4496179
ATAACGC6100.023.1137313
ACCTGGG42300.022.6656653
Produced by FastQC (version 0.11.5)