##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431343_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3957722 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.804235618368345 34.0 31.0 34.0 31.0 34.0 2 32.924541946099296 34.0 31.0 34.0 31.0 34.0 3 33.0017179074225 34.0 31.0 34.0 31.0 34.0 4 36.3859154331709 37.0 37.0 37.0 35.0 37.0 5 36.331362586861836 37.0 37.0 37.0 35.0 37.0 6 36.35987949633653 37.0 37.0 37.0 35.0 37.0 7 36.23402856491689 37.0 37.0 37.0 35.0 37.0 8 36.28927499202824 37.0 37.0 37.0 35.0 37.0 9 38.12925844715723 39.0 39.0 39.0 37.0 39.0 10-11 38.10951691402276 39.0 39.0 39.0 37.0 39.0 12-13 38.09006102500378 39.0 39.0 39.0 36.0 39.0 14-15 39.616832233289756 41.0 40.0 41.0 37.0 41.0 16-17 39.61510131333125 41.0 40.0 41.0 37.0 41.0 18-19 39.66128697265751 41.0 40.0 41.0 37.0 41.0 20-21 39.65163773504051 41.0 40.0 41.0 37.0 41.0 22-23 39.57764112790135 41.0 40.0 41.0 37.0 41.0 24-25 39.55687425746427 41.0 40.0 41.0 37.0 41.0 26-27 39.47687975557657 41.0 40.0 41.0 36.0 41.0 28-29 39.39894616145348 41.0 39.0 41.0 36.0 41.0 30-31 39.27835191557163 41.0 39.0 41.0 36.0 41.0 32-33 39.11671410978336 41.0 39.0 41.0 35.0 41.0 34-35 39.02869049417822 41.0 39.0 41.0 35.0 41.0 36-37 38.93379575422428 41.0 39.0 41.0 35.0 41.0 38-39 38.83089135618924 41.0 39.0 41.0 35.0 41.0 40-41 38.754177908402866 41.0 39.0 41.0 35.0 41.0 42-43 38.643107828190054 41.0 38.5 41.0 35.0 41.0 44-45 38.48672279154523 41.0 38.0 41.0 35.0 41.0 46-47 38.261331644819926 40.5 38.0 41.0 34.5 41.0 48-49 38.05714941574977 40.0 38.0 41.0 34.0 41.0 50-51 37.909691736812235 40.0 38.0 41.0 34.0 41.0 52-53 37.77903602122635 40.0 37.5 41.0 34.0 41.0 54-55 37.615756740872655 40.0 37.0 41.0 33.0 41.0 56-57 37.432261159323474 40.0 36.5 41.0 33.0 41.0 58-59 37.22774780542949 40.0 36.0 41.0 33.0 41.0 60-61 37.00361230526045 39.0 35.5 41.0 33.0 41.0 62-63 36.739569125875946 39.0 35.0 41.0 32.5 41.0 64-65 36.44367353240071 39.0 35.0 41.0 32.0 41.0 66-67 36.12776680625875 38.0 35.0 40.5 32.0 41.0 68-69 35.842576108175365 37.0 35.0 40.0 31.5 41.0 70-71 35.56987289658041 37.0 35.0 39.5 31.0 41.0 72-73 35.30218645978672 36.0 35.0 39.0 31.0 41.0 74-75 35.00255020438525 36.0 35.0 39.0 31.0 41.0 76-77 34.69225920870642 35.0 35.0 37.5 31.0 40.0 78-79 34.40259308258639 35.0 35.0 37.0 31.0 39.0 80-81 34.12173821203207 35.0 35.0 37.0 31.0 39.0 82-83 33.84198890169648 35.0 35.0 36.0 31.0 39.0 84-85 33.62081722768805 35.0 34.5 36.0 31.0 37.0 86-87 33.43505569618078 35.0 34.0 36.0 31.0 37.0 88-89 33.24950021754939 35.0 34.0 35.0 30.0 37.0 90-91 33.07855604815093 35.0 34.0 35.0 30.0 36.0 92-93 32.95322283879464 35.0 34.0 35.0 30.0 36.0 94-95 32.83321011430313 35.0 34.0 35.0 30.0 36.0 96-97 32.70658019941774 35.0 34.0 35.0 30.0 36.0 98-99 32.5930530239365 35.0 34.0 35.0 30.0 36.0 100 32.489760776527504 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24674299208309236 1101 2 0.21762510444465732 1101 3 0.2320371296459669 1101 4 0.17675209691848437 1101 5 0.1988593812484396 1101 6 0.22801547331743421 1101 7 0.26276886679080746 1101 8 0.2595886026140022 1101 9 0.3134646897867128 1101 10-11 0.294724879641322 1101 12-13 0.31102697279499125 1101 14-15 0.37839199606261076 1101 16-17 0.41122020913694257 1101 18-19 0.4018396426264559 1101 20-21 0.40999631041886886 1101 22-23 0.42084946205712015 1101 24-25 0.3989148941128704 1101 26-27 0.38384705553904297 1101 28-29 0.3857693294793023 1101 30-31 0.4110229576113369 1101 32-33 0.4197330193465021 1101 34-35 0.4320869311590698 1101 36-37 0.39037996190180024 1101 38-39 0.3569713287559537 1101 40-41 0.3941995069994988 1101 42-43 0.41258221154397035 1101 44-45 0.4378108078503047 1101 46-47 0.44416412779430203 1101 48-49 0.41710798358216294 1101 50-51 0.42403181148841895 1101 52-53 0.4276474272014852 1101 54-55 0.4475413350613451 1101 56-57 0.44394957466076335 1101 58-59 0.4555682593027015 1101 60-61 0.47264907211323504 1101 62-63 0.47982109350804336 1101 64-65 0.5117925908160288 1101 66-67 0.5271030546197011 1101 68-69 0.5043393860219396 1101 70-71 0.47744013234839144 1101 72-73 0.4899419833104872 1101 74-75 0.47490288955896176 1101 76-77 0.4315624580439916 1101 78-79 0.43853507283806437 1101 80-81 0.4340238888767942 1101 82-83 0.5024924988519288 1101 84-85 0.4191866185688511 1101 86-87 0.3872227280131568 1101 88-89 0.40564291319223145 1101 90-91 0.4193545565290151 1101 92-93 0.47038522482762346 1101 94-95 0.47925490466326437 1101 96-97 0.49052199371325145 1101 98-99 0.504949475215291 1101 100 0.5525540066694248 1102 1 0.1621571950512859 1102 2 0.19957908575874228 1102 3 0.24216348883647498 1102 4 0.14661884874011832 1102 5 0.16529594830890915 1102 6 0.1646580944105409 1102 7 0.21035346947059708 1102 8 0.20488518012942336 1102 9 0.22866929952019888 1102 10-11 0.243352558672143 1102 12-13 0.24272011553436812 1102 14-15 0.3372786771537406 1102 16-17 0.31984121330265936 1102 18-19 0.3483767938508606 1102 20-21 0.2886789901733806 1102 22-23 0.3252937342425568 1102 24-25 0.34351804332327873 1102 26-27 0.35478179607064675 1102 28-29 0.3482703614892131 1102 30-31 0.3571298044540612 1102 32-33 0.3463361142026713 1102 34-35 0.3119403373682559 1102 36-37 0.30688474970718005 1102 38-39 0.3271660589890857 1102 40-41 0.3460991979009407 1102 42-43 0.3396745460111248 1102 44-45 0.36787527938814435 1102 46-47 0.4035065403441962 1102 48-49 0.40390422745727506 1102 50-51 0.4553098443262087 1102 52-53 0.45375923386840356 1102 54-55 0.438861848618302 1102 56-57 0.42644014965653554 1102 58-59 0.43579474352854675 1102 60-61 0.4513111219638901 1102 62-63 0.45500364859781683 1102 64-65 0.42881853075830634 1102 66-67 0.4390127989962451 1102 68-69 0.4781654014013057 1102 70-71 0.43126842072315696 1102 72-73 0.39309086430291273 1102 74-75 0.38780773855403794 1102 76-77 0.42434157992246213 1102 78-79 0.4402215983607718 1102 80-81 0.3889678288631302 1102 82-83 0.4115753081507023 1102 84-85 0.37957360015244035 1102 86-87 0.31897516628455236 1102 88-89 0.3829389525183515 1102 90-91 0.41761887542203624 1102 92-93 0.38138592546198424 1102 94-95 0.42400639564736053 1102 96-97 0.4223370217128988 1102 98-99 0.4336731574184185 1102 100 0.48371323226154317 1103 1 0.16655699643386868 1103 2 0.18333807286141734 1103 3 0.18346271700843175 1103 4 0.16009739344398355 1103 5 0.15897709795867598 1103 6 0.17334533615554903 1103 7 0.17258593520472232 1103 8 0.18512051750762026 1103 9 0.2541077192769805 1103 10-11 0.25688658925371755 1103 12-13 0.25868996767661656 1103 14-15 0.3345852147556698 1103 16-17 0.3283551188599034 1103 18-19 0.3300639636929361 1103 20-21 0.30310253985046387 1103 22-23 0.3368631750811275 1103 24-25 0.36282292709027786 1103 26-27 0.33144084414510644 1103 28-29 0.33733752579728105 1103 30-31 0.35727513509804965 1103 32-33 0.34809465226788205 1103 34-35 0.3666647116519002 1103 36-37 0.4142695761036421 1103 38-39 0.42282017124315985 1103 40-41 0.39268986772104597 1103 42-43 0.38734923198816773 1103 44-45 0.428418910873269 1103 46-47 0.4222502289315031 1103 48-49 0.43008178687426124 1103 50-51 0.44559407230197223 1103 52-53 0.4394845896506254 1103 54-55 0.4580812926047457 1103 56-57 0.4838059227955753 1103 58-59 0.45123891145283324 1103 60-61 0.4634697126991796 1103 62-63 0.4262762462592278 1103 64-65 0.4371835477984831 1103 66-67 0.4482170844103237 1103 68-69 0.4632876129860577 1103 70-71 0.4345938460905998 1103 72-73 0.43318262151066733 1103 74-75 0.39379406330977673 1103 76-77 0.37696899125399597 1103 78-79 0.3061097244642639 1103 80-81 0.29728838604367525 1103 82-83 0.33680441992870414 1103 84-85 0.37745805493753437 1103 86-87 0.35258725727909024 1103 88-89 0.37174666316798266 1103 90-91 0.4329062597394824 1103 92-93 0.45784599016647576 1103 94-95 0.4248090687553372 1103 96-97 0.4809843576758013 1103 98-99 0.4584365978261076 1103 100 0.5126730992499446 1104 1 0.05579081635639227 1104 2 0.11930613987284744 1104 3 0.1459569550590416 1104 4 0.06665950174605939 1104 5 0.08875601666801458 1104 6 0.11027808049033183 1104 7 0.09609241873521768 1104 8 0.12750625952279648 1104 9 0.18081354410942652 1104 10-11 0.18202139464113998 1104 12-13 0.19461772462020122 1104 14-15 0.23537504817606703 1104 16-17 0.2851356852900153 1104 18-19 0.2436247876014619 1104 20-21 0.2610049099393663 1104 22-23 0.21107132371824378 1104 24-25 0.25329105787584894 1104 26-27 0.25224138225443227 1104 28-29 0.22624564557506233 1104 30-31 0.32022528830883346 1104 32-33 0.29485674403616713 1104 34-35 0.30416854509219604 1104 36-37 0.30593762654876144 1104 38-39 0.26210815853074365 1104 40-41 0.25414819719247816 1104 42-43 0.2530843149377944 1104 44-45 0.24587718336750441 1104 46-47 0.2741769265934977 1104 48-49 0.28273047216115543 1104 50-51 0.2269452722801759 1104 52-53 0.2322685801762816 1104 54-55 0.27351303700919516 1104 56-57 0.2947356228828113 1104 58-59 0.2573742989052761 1104 60-61 0.2676128819622434 1104 62-63 0.30125215615616696 1104 64-65 0.3115594084322524 1104 66-67 0.33564269127765556 1104 68-69 0.28666845723855516 1104 70-71 0.2923877643729327 1104 72-73 0.3035761921670499 1104 74-75 0.24802614237071907 1104 76-77 0.22232797720553776 1104 78-79 0.23079121639011646 1104 80-81 0.27160422223838765 1104 82-83 0.2973127001272289 1104 84-85 0.27040853140936605 1104 86-87 0.2711482207036937 1104 88-89 0.30709220874450693 1104 90-91 0.35543767541280147 1104 92-93 0.38254123857373656 1104 94-95 0.34352005262604024 1104 96-97 0.3662327005401309 1104 98-99 0.3580254058650496 1104 100 0.35394626388004014 1105 1 -0.007797338732693504 1105 2 0.04515563641710685 1105 3 0.06697701943981116 1105 4 -0.030682789857927162 1105 5 -0.03379511209429609 1105 6 -0.06385530166107145 1105 7 -0.4238617715728381 1105 8 -0.33800723579634706 1105 9 -0.30060410766384393 1105 10-11 -0.29325114698443144 1105 12-13 -0.20647608574648046 1105 14-15 -0.20418985692021607 1105 16-17 -0.1319373937229571 1105 18-19 -0.15335582859447783 1105 20-21 -0.11290606044408236 1105 22-23 -0.1482742945882194 1105 24-25 -0.13215735899623837 1105 26-27 -0.10169013369423396 1105 28-29 -0.14164274567531265 1105 30-31 -0.17831153007474398 1105 32-33 -0.1639482025243666 1105 34-35 -0.22347011803904593 1105 36-37 -0.23469763214423978 1105 38-39 -0.22179577482171453 1105 40-41 -0.23645345049500577 1105 42-43 -0.21073415181733424 1105 44-45 -0.18929584480626716 1105 46-47 -0.2155043781081929 1105 48-49 -0.18650093091515885 1105 50-51 -0.1900934086525652 1105 52-53 -0.19546153909372066 1105 54-55 -0.22842695641436706 1105 56-57 -0.1838106151332468 1105 58-59 -0.165790884174136 1105 60-61 -0.13468024565113268 1105 62-63 -0.1840682201782755 1105 64-65 -0.13464155681286627 1105 66-67 -0.16799869532644607 1105 68-69 -0.15230531587715035 1105 70-71 -0.18297101537751814 1105 72-73 -0.14666742203340988 1105 74-75 -0.1629989962127354 1105 76-77 -0.1682344819345971 1105 78-79 -0.13885866751149933 1105 80-81 -0.15029041819090594 1105 82-83 -0.1172657407107991 1105 84-85 -0.08616809002285919 1105 86-87 -0.1604091222564321 1105 88-89 -0.09431361358726775 1105 90-91 -0.08418198481966499 1105 92-93 -0.090782418599062 1105 94-95 -0.05225444444293714 1105 96-97 -0.1261279619707807 1105 98-99 -0.13820640104862036 1105 100 -0.14056986590450293 1106 1 0.06696236211209339 1106 2 0.06820654987492958 1106 3 0.0932459618738406 1106 4 0.032622542262615184 1106 5 0.03847634509833142 1106 6 0.0710447322257508 1106 7 0.09313151645916662 1106 8 0.07005978876268415 1106 9 0.09511283633967338 1106 10-11 0.11218920329825721 1106 12-13 0.08905235540044032 1106 14-15 0.12407953733805499 1106 16-17 0.14813036505045574 1106 18-19 0.11268008251504824 1106 20-21 0.10041408972802657 1106 22-23 0.11509157306294782 1106 24-25 0.1236893507126311 1106 26-27 0.10365668599816757 1106 28-29 0.09486859154608851 1106 30-31 0.09444673801159809 1106 32-33 0.09380687009448252 1106 34-35 0.1202211373783868 1106 36-37 0.10117101482051538 1106 38-39 0.10690270383850731 1106 40-41 0.10521452408394083 1106 42-43 0.09014387347293251 1106 44-45 0.12688279223024068 1106 46-47 0.2095720009961255 1106 48-49 0.2261337394612255 1106 50-51 0.24299324945322098 1106 52-53 0.22108786134740654 1106 54-55 0.23453246263478889 1106 56-57 0.22228219016022877 1106 58-59 0.23489981240805236 1106 60-61 0.2504295298041015 1106 62-63 0.20586525464739225 1106 64-65 0.2389275722959212 1106 66-67 0.2478929590042327 1106 68-69 0.20298612562052654 1106 70-71 0.21575666836929486 1106 72-73 0.21033937613789533 1106 74-75 0.26791233625262123 1106 76-77 0.244137758816386 1106 78-79 0.2810698877804896 1106 80-81 0.30654061440584 1106 82-83 0.31647389259816094 1106 84-85 0.27001901871815903 1106 86-87 0.2595220934480196 1106 88-89 0.27442833829345403 1106 90-91 0.31479458868797394 1106 92-93 0.3357425625253825 1106 94-95 0.32048096490388644 1106 96-97 0.24141210770527977 1106 98-99 0.1811949169702558 1106 100 0.19015644156188927 1107 1 -0.027097721055049817 1107 2 -0.016730839533941833 1107 3 -0.008676213331270333 1107 4 -0.09231793128307686 1107 5 -0.09954533795172438 1107 6 -0.06558584829766545 1107 7 -0.08655685503818233 1107 8 -0.044866570936534345 1107 9 0.014364149058081921 1107 10-11 -2.573013031152982E-4 1107 12-13 0.033970299065288145 1107 14-15 -0.005171187303410818 1107 16-17 0.011364108643689974 1107 18-19 -0.00627980541055706 1107 20-21 0.006537029171788333 1107 22-23 0.006486687581130468 1107 24-25 -0.005453533687123979 1107 26-27 -0.04564354165294304 1107 28-29 -0.040318730560635174 1107 30-31 -0.025417782130688238 1107 32-33 -0.08086460399223228 1107 34-35 -0.09200367528643483 1107 36-37 -0.059676077335062416 1107 38-39 -0.04294299784182698 1107 40-41 -0.07600734296885037 1107 42-43 -0.12177594942443193 1107 44-45 -0.07357424809615765 1107 46-47 -0.10462976950750402 1107 48-49 -0.11502677156456542 1107 50-51 -0.03744157463336251 1107 52-53 -0.07940305221051602 1107 54-55 -0.10855733279284863 1107 56-57 -0.05438346588532994 1107 58-59 -0.12207313343921555 1107 60-61 -0.1165390120003238 1107 62-63 -0.11156798555197867 1107 64-65 -0.14086857410126186 1107 66-67 -0.10722592401550202 1107 68-69 -0.13762582723922634 1107 70-71 -0.15655653879321818 1107 72-73 -0.15821607167616492 1107 74-75 -0.16782643213132076 1107 76-77 -0.15714951546008393 1107 78-79 -0.11368901374406448 1107 80-81 -0.15353898507456165 1107 82-83 -0.12665559148847905 1107 84-85 -0.09409348170655818 1107 86-87 -0.1399320535398445 1107 88-89 -0.1829456498875075 1107 90-91 -0.15003216384663887 1107 92-93 -0.16553441387051748 1107 94-95 -0.17944701527400042 1107 96-97 -0.10353422047301564 1107 98-99 -0.07237150827994299 1107 100 -0.0798654087633821 1108 1 -0.14915851581551465 1108 2 -0.14566940452341726 1108 3 -0.09720932862371967 1108 4 -0.07573915342607762 1108 5 -0.261967090716432 1108 6 -0.26070871350325575 1108 7 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2214 80-81 -0.19464186178372245 2214 82-83 -0.1774095839352796 2214 84-85 -0.22117613400531866 2214 86-87 -0.20981888254923575 2214 88-89 -0.19702293313414287 2214 90-91 -0.20942928513139236 2214 92-93 -0.2448129763890563 2214 94-95 -0.2379002489041966 2214 96-97 -0.20525838508599037 2214 98-99 -0.2226466239594913 2214 100 -0.25760416125641683 2215 1 -0.23431257052745735 2215 2 -0.1977557885643506 2215 3 -0.21570119785737063 2215 4 -0.17123879956671573 2215 5 -0.17547199720866757 2215 6 -0.19376141809586755 2215 7 -0.23807083848979715 2215 8 -0.21264580551643775 2215 9 -0.28173467167169974 2215 10-11 -0.2920974716232507 2215 12-13 -0.2785315958867187 2215 14-15 -0.39909949304254866 2215 16-17 -0.3733037515654303 2215 18-19 -0.3774848636197845 2215 20-21 -0.38009765255392836 2215 22-23 -0.36778042817991263 2215 24-25 -0.34400533372512854 2215 26-27 -0.3520682793361303 2215 28-29 -0.36023016512618256 2215 30-31 -0.3311558539162718 2215 32-33 -0.3774569903893976 2215 34-35 -0.3744057367723599 2215 36-37 -0.3555112224273742 2215 38-39 -0.3760697351892688 2215 40-41 -0.38607990946225357 2215 42-43 -0.44085674795904595 2215 44-45 -0.38984076560699066 2215 46-47 -0.4111800824241669 2215 48-49 -0.39680983080464927 2215 50-51 -0.3931683414126539 2215 52-53 -0.3883659625461888 2215 54-55 -0.4665193109970147 2215 56-57 -0.4789042463911031 2215 58-59 -0.4521313426670588 2215 60-61 -0.402029845239376 2215 62-63 -0.4127523490366798 2215 64-65 -0.3488035053320715 2215 66-67 -0.38163868301384696 2215 68-69 -0.38233960444611625 2215 70-71 -0.3930619847928227 2215 72-73 -0.37910840734407714 2215 74-75 -0.4009223048704129 2215 76-77 -0.3266611059108655 2215 78-79 -0.46356245868174994 2215 80-81 -0.4351372171675436 2215 82-83 -0.3637400608686434 2215 84-85 -0.3593188663725684 2215 86-87 -0.3725381042971989 2215 88-89 -0.37892482057517185 2215 90-91 -0.4252939537757783 2215 92-93 -0.45431788796642536 2215 94-95 -0.49447700728008215 2215 96-97 -0.508489344312288 2215 98-99 -0.5845071968260029 2215 100 -0.49755701336805913 2216 1 -0.28114905286738434 2216 2 -0.296291244832922 2216 3 -0.3916711161704498 2216 4 -0.22010531362786168 2216 5 -0.2917762140803575 2216 6 -0.23798847671777423 2216 7 -0.37192515765108425 2216 8 -0.38676424944441123 2216 9 -0.37762568153455334 2216 10-11 -0.3827457602512965 2216 12-13 -0.4173731457760681 2216 14-15 -0.5804486918868932 2216 16-17 -0.6129850170892297 2216 18-19 -0.5758959484872292 2216 20-21 -0.5344458768336153 2216 22-23 -0.5903930658132381 2216 24-25 -0.6184305355375344 2216 26-27 -0.6442129473187137 2216 28-29 -0.5828040151328864 2216 30-31 -0.6707956350665043 2216 32-33 -0.6339842562522193 2216 34-35 -0.6638418376451796 2216 36-37 -0.6968577175202313 2216 38-39 -0.7075626262841226 2216 40-41 -0.7098173765934632 2216 42-43 -0.664742489475735 2216 44-45 -0.7147311504316107 2216 46-47 -0.6311109161182316 2216 48-49 -0.6721692336257945 2216 50-51 -0.670202237303748 2216 52-53 -0.6988680933265101 2216 54-55 -0.7599750532935445 2216 56-57 -0.7695577942594056 2216 58-59 -0.9256737451020172 2216 60-61 -0.8530699665299437 2216 62-63 -0.8645445825455482 2216 64-65 -0.8846550633046562 2216 66-67 -0.8157825755941275 2216 68-69 -0.8131042162616495 2216 70-71 -0.8523039233873106 2216 72-73 -0.8819017585436484 2216 74-75 -0.8017043439753451 2216 76-77 -0.7677953226564682 2216 78-79 -0.7365345108104577 2216 80-81 -0.7284174666131307 2216 82-83 -0.7598774203167622 2216 84-85 -0.7614041580578643 2216 86-87 -0.7400585131847421 2216 88-89 -0.6775795641180409 2216 90-91 -0.6873363006414053 2216 92-93 -0.8655366004483795 2216 94-95 -0.8485712353543562 2216 96-97 -0.9483577243822339 2216 98-99 -0.8806989990421457 2216 100 -0.8948873039302825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59.0 3 251.0 4 124.0 5 167.0 6 266.0 7 513.0 8 1010.0 9 1570.0 10 2226.0 11 3310.0 12 5772.0 13 8566.0 14 7665.0 15 8655.0 16 11364.0 17 17536.0 18 20610.0 19 11892.0 20 7702.0 21 7024.0 22 7456.0 23 7992.0 24 9191.0 25 11484.0 26 14480.0 27 18747.0 28 23859.0 29 29772.0 30 38053.0 31 47955.0 32 61731.0 33 80245.0 34 115208.0 35 189076.0 36 360147.0 37 828565.0 38 1360278.0 39 595614.0 40 41587.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.81644153107867 20.617310436441365 13.430053573147777 25.136194459332188 2 14.375441226038616 21.81153704075223 38.6916261425133 25.12139559069586 3 15.11933316842278 28.19715284201611 31.007926066168213 25.6755879233929 4 11.151163393011682 17.637763243173584 37.937641578682054 33.27343178513267 5 13.548955687135175 35.93405499426185 34.144060649029925 16.372928669573053 6 30.225922892007066 37.77390124925399 17.863508351521407 14.136667507217535 7 25.68073250218181 31.9001941015564 24.57514701638973 17.84392637987206 8 24.825973122922733 33.825847292962976 22.246458947849295 19.101720636265 9 25.360826253081946 15.052396302721615 21.72722086088917 37.85955658330727 10-11 24.408978195032393 25.988864301231867 28.40762943935931 21.194528064376428 12-13 24.449986633725157 24.80986537204988 29.403429548614074 21.336718445610884 14-15 22.70503082328673 24.615005803843726 26.740458273724126 25.939505099145414 16-17 22.23071756934924 28.60343652232269 26.82982786562573 22.336018042702342 18-19 21.376943605437674 27.00964089948713 29.767022544787125 21.84639295028807 20-21 23.716079603367795 26.25371362617182 28.230583148589012 21.79962362187137 22-23 22.116777098528583 26.9288159507009 29.529126558868057 21.425280391902458 24-25 22.57552898525641 25.60999151278805 28.858314599899586 22.956164902055956 26-27 22.646566447722243 26.748240108557958 28.74655908233605 21.858634361383746 28-29 21.799829041558873 26.223726130944662 29.583857945309976 22.39258688218649 30-31 23.219109098470962 26.529782542890306 28.052780763553358 22.19832759508537 32-33 21.55904733869103 26.95821525718596 29.65807084947865 21.824666554644356 34-35 22.440425187296132 25.30241922747753 28.977185866946908 23.279969718279432 36-37 22.965986339552405 26.80800301802349 28.355397029025735 21.87061361339837 38-39 22.104505391379757 26.381703723506135 28.98425667586581 22.5295342092483 40-41 23.069715871677072 26.356018756926584 27.95516601315249 22.619099358243854 42-43 21.85976309995975 27.029719006201326 29.17005141618762 21.940466477651306 44-45 22.55877844233306 25.711138865720272 28.438732486360134 23.291350205586525 46-47 22.962878081333017 26.9234034628729 27.824736878411333 22.28898157738275 48-49 22.13430610567175 26.526942241979306 28.427640982018627 22.911110670330313 50-51 23.401748475399646 26.49625354674643 27.04394864006697 23.058049337786958 52-53 22.13303791082824 27.240648438754473 28.262473749100337 22.363839901316958 54-55 22.8422579567638 26.298253380370728 27.54474692637293 23.31474173649254 56-57 22.75656724546803 27.1112912947589 27.534151546211106 22.597989913561964 58-59 22.102103156800798 26.150168020843456 28.288307318391382 23.459421503964368 60-61 23.571839794547625 26.65390337911702 26.899285457192306 22.874971369143054 62-63 22.173550845663236 27.113753821011176 28.412240677844476 22.300454655481108 64-65 22.742287103540875 25.703839228728043 27.850250725038293 23.703622942692792 66-67 22.872466762109738 26.927226593748564 27.555144652854878 22.64516199128682 68-69 22.509898450864014 26.487193286993172 27.936713082631538 23.066195179511283 70-71 23.650774459420994 26.319340241758233 26.98308319003424 23.04680210878653 72-73 22.249750708646914 26.976817578107188 28.335251736575 22.438179976670902 74-75 22.779248212632037 25.697518846482808 27.75170019468698 23.771532746198172 76-77 22.989652208748783 27.04282242395813 27.3445377238245 22.62298764346858 78-79 22.343099839400697 26.675010789160105 27.896826319284347 23.08506305215485 80-81 23.32159878717054 26.528332517410735 26.95262069074428 23.19744800467445 82-83 22.076997683861276 27.006571678827246 28.375194511813724 22.54123612549775 84-85 22.736265610491007 25.809673608873894 27.400013897048144 24.054046883586956 86-87 22.92778683042454 27.003511876682317 27.358944420679315 22.709756872213827 88-89 21.966258584463517 26.40328871225246 28.006686276746716 23.62376642653731 90-91 23.054085126274433 26.416939345318564 26.90848104304322 23.62049448536379 92-93 22.07382835828344 27.077216145094052 28.13991869058976 22.70903680603275 94-95 22.584331752522253 25.68245279486203 27.604364989224727 24.12885046339099 96-97 22.612792969120132 26.919856267404686 27.436377910298265 23.030972853176916 98-99 21.73662729041692 26.3425771629543 28.039362436770887 23.88143310985789 100 22.90464594877118 25.918434436019815 27.078538206584486 24.09838140862452 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 874.0 1 596.0 2 1935.5 3 2838.0 4 1351.5 5 651.0 6 1143.0 7 2678.5 8 4601.5 9 4952.0 10 3252.0 11 1818.5 12 1674.5 13 1776.5 14 1946.0 15 2155.5 16 2426.0 17 2811.5 18 3181.0 19 3651.0 20 4480.5 21 5350.5 22 6396.5 23 8049.5 24 10710.5 25 13734.5 26 17377.0 27 22543.0 28 29204.5 29 37176.0 30 44350.0 31 51226.0 32 58393.0 33 64277.0 34 70628.5 35 79052.5 36 88381.5 37 99016.0 38 114278.0 39 130912.0 40 145383.5 41 157046.0 42 162431.0 43 164308.0 44 163137.0 45 156845.5 46 147329.5 47 137696.0 48 129605.5 49 126782.0 50 127141.0 51 124382.5 52 120241.5 53 117339.0 54 113047.5 55 106478.5 56 99971.5 57 91983.0 58 85162.0 59 79874.5 60 72417.5 61 63024.5 62 53903.5 63 44960.0 64 36266.0 65 29419.0 66 23477.5 67 18330.0 68 14297.5 69 11003.5 70 8313.0 71 6228.0 72 4866.0 73 3899.5 74 3123.0 75 2321.0 76 1525.0 77 1056.0 78 738.0 79 431.0 80 218.5 81 109.5 82 57.0 83 27.5 84 17.5 85 15.0 86 15.0 87 11.0 88 6.5 89 8.0 90 6.0 91 2.0 92 1.5 93 2.0 94 1.5 95 4.5 96 6.5 97 7.0 98 5.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008312862803400543 2 0.0 3 5.306082640468431E-4 4 0.0029562460425466975 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.316765048176704E-5 24-25 7.580118057812044E-5 26-27 5.179747339504897E-4 28-29 8.843471067447385E-4 30-31 0.0017181600931040633 32-33 0.004472269654109106 34-35 0.005533486182202793 36-37 0.004169064931796624 38-39 0.002728842500812336 40-41 0.0036384566677497817 42-43 4.8007414366142947E-4 44-45 5.306082640468431E-4 46-47 0.0035626554871716607 48-49 0.0024888054289816212 50-51 0.00114965123876816 52-53 1.2633530096353407E-5 54-55 5.053412038541363E-4 56-57 7.959123960702647E-4 58-59 7.201112154921443E-4 60-61 4.1690649317966246E-4 62-63 0.0 64-65 0.0 66-67 0.0010106824077082727 68-69 8.08545926166618E-4 70-71 0.0022866689474399667 72-73 8.843471067447385E-5 74-75 0.002198234236765493 76-77 4.4217355337236927E-4 78-79 0.0011622847688645135 80-81 8.717135766483852E-4 82-83 0.0014528559610806418 84-85 0.0011875518290572202 86-87 2.2740354173436135E-4 88-89 0.0016044583222368828 90-91 0.0018192283338748908 92-93 0.0031204819337992914 94-95 0.001238085949442634 96-97 0.0024003707183071475 98-99 0.00229930247753632 100 0.003663723727942488 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3957722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.22205535021702 #Duplication Level Percentage of deduplicated Percentage of total 1 79.31101701875983 48.55583473804517 2 12.923698652405877 15.824307884542351 3 3.4419241679191415 6.32165015758786 4 1.4233091198382877 3.4855163886083327 5 0.7266658846959838 2.2243989506985966 6 0.4690519413691755 1.7229794349978245 7 0.32273285632306736 1.3830858155201127 8 0.23021520180267563 1.1275398261779832 9 0.16056320867612844 0.884700868390054 >10 0.8871905077921353 9.880316556321038 >50 0.06540740066661428 2.7556819840044344 >100 0.03558074609134518 3.874681792118271 >500 0.002230279046725894 0.9478308921420221 >1k 2.891102290249687E-4 0.3516974589559321 >5k 8.260292257856222E-5 0.36868938450186467 >10k+ 4.130146128928111E-5 0.2910878673880712 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11512 0.29087439693844086 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8801 0.22237539675601267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5780 0.14604360791384538 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4805 0.12140822422595625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 2.5267060192706814E-5 10-11 0.0 0.0 0.0 0.0 2.5267060192706814E-5 12-13 0.0 0.0 0.0 0.0 2.5267060192706814E-5 14-15 0.0 1.2633530096353407E-5 0.0 0.0 2.5267060192706814E-5 16-17 0.0 2.5267060192706814E-5 0.0 1.2633530096353407E-5 2.5267060192706814E-5 18-19 0.0 2.5267060192706814E-5 0.0 2.5267060192706814E-5 2.5267060192706814E-5 20-21 0.0 2.5267060192706814E-5 0.0 8.843471067447386E-5 2.5267060192706814E-5 22-23 0.0 2.5267060192706814E-5 0.0 3.41105312601542E-4 2.5267060192706814E-5 24-25 0.0 2.5267060192706814E-5 0.0 5.811423844322567E-4 2.5267060192706814E-5 26-27 0.0 2.5267060192706814E-5 0.0 0.0012001853591535737 2.5267060192706814E-5 28-29 0.0 2.5267060192706814E-5 0.0 0.0027793766211977496 2.5267060192706814E-5 30-31 2.5267060192706814E-5 2.5267060192706814E-5 0.0 0.005419784411335612 2.5267060192706814E-5 32-33 2.5267060192706814E-5 2.5267060192706814E-5 0.0 0.0113070094362363 2.5267060192706814E-5 34-35 2.5267060192706814E-5 2.5267060192706814E-5 0.0 0.020580020526959703 2.5267060192706814E-5 36-37 2.5267060192706814E-5 3.790059028906022E-5 0.0 0.03469167364458646 2.5267060192706814E-5 38-39 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.05220174635813228 2.5267060192706814E-5 40-41 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.071531047405553 2.5267060192706814E-5 42-43 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.09113828611509348 2.5267060192706814E-5 44-45 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.11730232694464139 2.5267060192706814E-5 46-47 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.14877245041465773 5.053412038541363E-5 48-49 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.17952246266918193 5.053412038541363E-5 50-51 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.21345612450798718 5.053412038541363E-5 52-53 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.24493888150809984 5.053412038541363E-5 54-55 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.28285210532725646 5.053412038541363E-5 56-57 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.3302910108390635 5.053412038541363E-5 58-59 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.38150734184968016 5.053412038541363E-5 60-61 2.5267060192706814E-5 5.053412038541363E-5 0.0 0.4364379307086248 5.053412038541363E-5 62-63 5.053412038541363E-5 5.053412038541363E-5 0.0 0.4925939719869157 5.053412038541363E-5 64-65 5.053412038541363E-5 5.053412038541363E-5 0.0 0.5568860066472583 5.053412038541363E-5 66-67 5.053412038541363E-5 5.053412038541363E-5 0.0 0.6346453843903135 5.053412038541363E-5 68-69 5.053412038541363E-5 5.053412038541363E-5 0.0 0.7238507404006649 5.053412038541363E-5 70-71 5.053412038541363E-5 5.053412038541363E-5 0.0 0.8223543745619324 5.053412038541363E-5 72-73 5.053412038541363E-5 7.580118057812044E-5 0.0 0.9245596330414314 5.053412038541363E-5 74-75 5.053412038541363E-5 7.580118057812044E-5 0.0 1.036833815007724 5.053412038541363E-5 76-77 5.053412038541363E-5 8.843471067447385E-5 0.0 1.1605792423015058 5.053412038541363E-5 78-79 5.053412038541363E-5 1.0106824077082725E-4 0.0 1.3034013000407811 5.053412038541363E-5 80-81 5.053412038541363E-5 1.0106824077082725E-4 0.0 1.465198919984779 5.053412038541363E-5 82-83 5.053412038541363E-5 1.0106824077082725E-4 0.0 1.6283988617694725 5.053412038541363E-5 84-85 5.053412038541363E-5 1.0106824077082725E-4 0.0 1.8032721853631963 5.053412038541363E-5 86-87 5.053412038541363E-5 1.0106824077082725E-4 0.0 1.997158971751932 5.053412038541363E-5 88 5.053412038541363E-5 1.0106824077082725E-4 0.0 2.156619388628105 5.053412038541363E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5610 0.0 51.27499 1 GTATCAA 10395 0.0 42.683945 1 TATAACG 325 0.0 37.59834 2 CAACGCA 13815 0.0 32.283676 5 TACCTGG 3105 0.0 31.483309 2 TCAACGC 14270 0.0 31.353905 4 ATCAACG 14270 0.0 31.320969 3 AACGCAG 14460 0.0 31.20115 6 TATCAAC 14890 0.0 29.764297 2 GTACCTG 3355 0.0 29.42008 1 TAACGCA 475 0.0 27.704039 4 ACGCAGA 16665 0.0 27.072826 7 CGCAGAG 16985 0.0 26.5351 8 TACATGG 13810 0.0 24.570963 2 GTACATG 14345 0.0 24.475813 1 GTATAAC 905 0.0 23.89056 1 ACATGGG 14025 0.0 23.457073 3 GCAGAGT 19280 0.0 23.449617 9 ATAACGC 610 0.0 23.113731 3 ACCTGGG 4230 0.0 22.665665 3 >>END_MODULE