FastQCFastQC Report
Fri 27 May 2016
SRR2140412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31441964
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26150.014.02521742
TCGTATG28250.013.62083843
CGTATGC31250.012.45721244
TCTCGTA30950.012.35894141
ATCTCGT38400.09.90242540
GATCTCG43400.09.07265539
GATCGGA42950.08.8041121
ACACGTC47350.08.3623113
CGTCTGA50300.08.22972916
GTATGCC51300.07.93936945
ACGTCTG53800.07.694337415
CCGGAAT38800.07.48337461
CCCGGAT46700.07.18139551
ATTACGG21850.07.10532239
CACGTCT58000.07.059550314
AATTACG22650.07.05355438
CGTCGAT10900.07.0208861
CTCGTTT41450.06.95064161
CCCCCGT32250.06.83968071
TTACGGC22750.06.82423240
Produced by FastQC (version 0.11.5)