##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31441964
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.785299957725286	34.0	31.0	34.0	31.0	34.0
2	33.004061387513836	34.0	31.0	34.0	31.0	34.0
3	33.08321512612889	34.0	33.0	34.0	31.0	34.0
4	36.42154249651835	37.0	37.0	37.0	35.0	37.0
5	36.30590356887375	37.0	37.0	37.0	35.0	37.0
6	36.26844630316351	37.0	37.0	37.0	35.0	37.0
7	36.28607414600437	37.0	37.0	37.0	35.0	37.0
8	36.26630690118467	37.0	37.0	37.0	35.0	37.0
9	38.062879564393626	39.0	39.0	39.0	37.0	39.0
10	38.010296939465995	39.0	38.0	39.0	35.0	39.0
11	38.06494110864067	39.0	39.0	39.0	37.0	39.0
12	38.0413169800716	39.0	38.0	39.0	35.0	39.0
13	38.00628799778538	39.0	38.0	39.0	35.0	39.0
14	39.537137215728634	41.0	40.0	41.0	37.0	41.0
15	39.526797117381086	41.0	40.0	41.0	37.0	41.0
16	39.507203398617214	41.0	40.0	41.0	37.0	41.0
17	39.487463919238635	41.0	40.0	41.0	37.0	41.0
18	39.477128718803954	41.0	40.0	41.0	37.0	41.0
19	39.48613658485201	41.0	40.0	41.0	37.0	41.0
20	39.47523230419067	41.0	40.0	41.0	37.0	41.0
21	39.437225995170024	41.0	39.0	41.0	37.0	41.0
22	39.40727179129141	41.0	39.0	41.0	36.0	41.0
23	39.36590592750504	41.0	39.0	41.0	36.0	41.0
24	39.35594096475653	41.0	39.0	41.0	36.0	41.0
25	39.31673495332544	41.0	39.0	41.0	36.0	41.0
26	39.26156762980837	41.0	39.0	41.0	36.0	41.0
27	39.19743741198864	41.0	39.0	41.0	36.0	41.0
28	39.148811410126925	40.0	39.0	41.0	36.0	41.0
29	39.10989170396608	40.0	39.0	41.0	36.0	41.0
30	39.046484596191256	40.0	39.0	41.0	36.0	41.0
31	39.0054585330611	40.0	39.0	41.0	35.0	41.0
32	38.948402459846335	40.0	39.0	41.0	35.0	41.0
33	38.89647761825565	40.0	38.0	41.0	35.0	41.0
34	38.80947513329638	40.0	38.0	41.0	35.0	41.0
35	38.78054236688268	40.0	38.0	41.0	35.0	41.0
36	38.707901357561504	40.0	38.0	41.0	35.0	41.0
37	38.65019045884029	40.0	38.0	41.0	35.0	41.0
38	38.58017886541693	40.0	38.0	41.0	35.0	41.0
39	38.5178242046203	40.0	38.0	41.0	34.0	41.0
40	38.45530307839548	40.0	38.0	41.0	34.0	41.0
41	38.36984432651853	40.0	38.0	41.0	34.0	41.0
42	38.28513686358778	40.0	38.0	41.0	34.0	41.0
43	38.20515967132333	40.0	38.0	41.0	34.0	41.0
44	38.134072159105585	40.0	38.0	41.0	33.0	41.0
45	38.049232897792265	40.0	38.0	41.0	33.0	41.0
46	37.942858276919345	40.0	37.0	41.0	33.0	41.0
47	38.145809180368	40.0	38.0	41.0	33.0	41.0
48	38.15190962625617	40.0	38.0	41.0	34.0	41.0
49	38.14440255704128	40.0	37.0	41.0	34.0	41.0
50	38.07531307522647	40.0	37.0	41.0	33.0	41.0
51	38.00711634934764	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1268.0
3	69.0
4	133.0
5	221.0
6	259.0
7	325.0
8	429.0
9	540.0
10	740.0
11	931.0
12	1317.0
13	1845.0
14	2448.0
15	3394.0
16	4483.0
17	5498.0
18	6256.0
19	7751.0
20	8705.0
21	10040.0
22	12955.0
23	17293.0
24	26188.0
25	39864.0
26	61440.0
27	90432.0
28	128506.0
29	177016.0
30	236076.0
31	310478.0
32	406212.0
33	536863.0
34	749298.0
35	1075629.0
36	1636211.0
37	2762808.0
38	6055531.0
39	1.7041629E7
40	20883.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.119034920075705	12.74873624471106	13.68540717029865	42.44682166491459
2	23.30495957568045	19.33543019132011	31.473205681426265	25.88640455157318
3	26.43472271643082	20.653099151185337	22.86672995363776	30.04544817874609
4	28.20722013421299	26.489398054141912	19.588709534811503	25.7146722768336
5	29.337747476588934	28.620457678788767	21.25518940229052	20.786605442331783
6	25.040216953368432	32.95031124646031	20.789203880520947	21.22026791965031
7	21.540015757285392	20.4163009664409	36.34564940027283	21.698033876000874
8	22.399033342828076	23.557974940751155	27.933840265194632	26.109151451226136
9	22.343947725402906	21.825872582259812	30.512674081046594	25.31750561129069
10	23.168517717277457	33.776058009607794	23.854619895881825	19.200804377232924
11	27.61929248440078	25.960464810658774	20.445993768073777	25.97424893686667
12	24.504885295439788	23.53059321530953	25.93967108736983	26.02485040188085
13	23.743532687716325	25.431267588754952	27.172933599186106	23.652266124342614
14	24.12672121881445	25.38280687555014	26.58173643351287	23.908735472122544
15	24.402489615470586	25.45528962503742	25.80962499670822	24.332595762783775
16	24.83958699272094	25.26780133709205	25.60344512830051	24.289166541886505
17	24.958348657863738	25.59754218915841	25.41556246295556	24.02854669002229
18	24.56691636692924	25.853318196026176	25.480965502027797	24.098799935016782
19	24.751997044459436	25.408400060505127	25.43729774641304	24.402305148622393
20	24.719305066312018	25.569528671936652	25.50886134212227	24.202304919629068
21	24.431996866347532	25.58711139121855	25.748361167949625	24.232530574484294
22	24.601500720502063	25.603203413120124	25.49438705546511	24.300908810912702
23	24.673191519055262	25.680499226935105	25.5915028602272	24.054806393782428
24	24.39876033149848	25.846062239007157	25.671505657695448	24.083671771798915
25	24.737293243365738	25.589042315833332	25.43314053805626	24.240523902744666
26	24.636029128674334	25.8642949512204	25.445028216212673	24.054647703892595
27	24.391540800181886	25.810625286387907	25.576495717007813	24.221338196422394
28	24.678018743656658	25.48096478976907	25.457515815970215	24.383500650604056
29	24.67566141997286	25.56996593848125	25.4676035792283	24.286769062317596
30	24.413027566667367	25.67099796915625	25.590173905965514	24.325800558210865
31	24.543838360604266	25.531047820034498	25.39694287544105	24.52817094392019
32	24.530327095963937	25.839348982322097	25.454161063224888	24.17616285848907
33	24.364004888910745	25.75240032272969	25.572867143347978	24.310727645011593
34	24.662913989814502	25.61443684942322	25.310234525233493	24.412414635528783
35	24.67912859680753	25.719647760670895	25.359593684017344	24.241629958504234
36	24.43389158006404	25.807550809519675	25.48872140751867	24.26983620289761
37	24.65894833690693	25.505077993064614	25.39721155557145	24.43876211445701
38	24.616742226944073	25.801966283780782	25.45580689937627	24.125484589898875
39	24.412914425882033	25.71707016693448	25.637979534273033	24.23203587291046
40	24.56206031936814	25.510543588663836	25.513778203688194	24.41361788827983
41	24.625474028403776	25.62195500310175	25.488633098238672	24.2639378702558
42	24.372429872981183	25.707176752982242	25.692341955815333	24.22805141822124
43	24.583073697827814	25.47073211948546	25.527723378588657	24.418470804098067
44	24.6470251030663	25.590056655616443	25.55909478232483	24.20382345899242
45	24.448645262637452	25.605107858489713	25.65471193393714	24.29153494493569
46	24.614879781220715	25.460673713449257	25.438581285741762	24.48586521958827
47	24.604150980207493	25.748808067649918	25.47725241968652	24.169788532456067
48	24.352750960889818	25.73876818634327	25.6746139750137	24.23386687775321
49	24.619438696193242	25.42555570849352	25.494705439419374	24.460300155893865
50	24.602051711799245	25.68612226266373	25.467376782354528	24.244449243182498
51	24.31650412951269	25.648234720907993	25.729631314966877	24.30562983461244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	299.0
1	282.5
2	266.0
3	329.5
4	393.0
5	623.5
6	854.0
7	1248.0
8	1642.0
9	2477.0
10	3312.0
11	5093.5
12	6875.0
13	9779.0
14	12683.0
15	18460.0
16	24237.0
17	33810.0
18	43383.0
19	59169.0
20	74955.0
21	99479.0
22	124003.0
23	157761.5
24	191520.0
25	234210.0
26	329611.0
27	382322.0
28	446147.5
29	509973.0
30	580090.0
31	650207.0
32	734598.5
33	818990.0
34	903072.5
35	987155.0
36	1075220.0
37	1163285.0
38	1264470.0
39	1365655.0
40	1459348.5
41	1553042.0
42	1625160.0
43	1697278.0
44	1769359.0
45	1841440.0
46	1918572.0
47	1995704.0
48	2054969.5
49	2114235.0
50	2152338.5
51	2190442.0
52	2193377.0
53	2196312.0
54	2154938.0
55	2113564.0
56	2032233.0
57	1950902.0
58	1834576.0
59	1718250.0
60	1578989.5
61	1439729.0
62	1287038.5
63	1134348.0
64	992813.0
65	851278.0
66	728627.5
67	605977.0
68	512451.0
69	418925.0
70	352702.5
71	286480.0
72	243938.0
73	201396.0
74	173128.5
75	125572.0
76	106283.0
77	93692.0
78	81101.0
79	70480.0
80	59859.0
81	50733.5
82	41608.0
83	34700.5
84	27793.0
85	22305.5
86	16818.0
87	12958.5
88	9099.0
89	6626.5
90	4154.0
91	2798.0
92	1442.0
93	979.5
94	517.0
95	333.5
96	150.0
97	94.5
98	39.0
99	34.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04642839741181562
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.8446684819052651E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.9082777399019983E-4
22	0.0
23	7.346869298622693E-4
24	3.180462899836664E-5
25	0.0032249893804343773
26	0.002045037644594975
27	0.0094205311093162
28	0.011093454594630285
29	0.008501377331263403
30	0.010517790809759849
31	0.0029133040162503845
32	0.00466255861116055
33	0.008428226684567159
34	0.0075122533694142015
35	0.00448127222586986
36	0.008205594281578594
37	0.015705125799393448
38	0.010390572293766382
39	0.006628084683259608
40	0.002604799114966228
41	6.042879509689662E-5
42	0.007537697072612894
43	0.006535851259164345
44	2.162714771888932E-4
45	0.0031772824369368275
46	0.004220474268083254
47	0.005187334989633599
48	0.006663069775157812
49	0.01855164009474726
50	0.009996194894186636
51	0.0026874911503619813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.1441964E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.65071511952895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15408328376203	78.11915787483551
2	7.287970499670882	12.63015711133025
3	1.572010526053203	4.0864750877381075
4	0.5001206266399125	1.7334323977750143
5	0.2007117694162107	0.8695909176410327
6	0.10564991844961194	0.5492784591587274
7	0.048328610617908566	0.2931396069542526
8	0.0305183245179073	0.2115547714981217
9	0.021185639940229303	0.16521757659771516
>10	0.07611184176877787	1.10772721005327
>50	0.002887732762133337	0.1644146609391594
>100	3.9524126861474845E-4	0.05103151840536558
>500	1.9683456675928696E-5	0.011189101892216028
>1k	6.301675859714634E-6	0.0076337051813465204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.633110959607994E-5	0.0	0.0	3.180462899836664E-6	3.180462899836664E-6
2	1.144966643941199E-4	6.042879509689662E-5	0.0	1.2721851599346656E-5	6.360925799673328E-6
3	2.767002722857898E-4	6.678972089656995E-5	0.0	1.590231449918332E-5	2.226324029885665E-5
4	7.728524846603094E-4	6.997018379640661E-5	0.0	2.8624166098529976E-5	4.134601769787663E-5
5	0.0011322447923418524	7.633110959607994E-5	0.0	2.8624166098529976E-5	6.042879509689662E-5
6	0.0016124946902171886	8.90529611954266E-5	0.0	3.4985091898203306E-5	6.678972089656995E-5
7	0.0019369019060005285	9.859434989493659E-5	0.0	4.134601769787663E-5	9.541388699509993E-5
8	0.0024139713409760282	1.240380530936299E-4	0.0	4.134601769787663E-5	9.541388699509993E-5
9	0.0029991765145459745	1.3039897889330324E-4	0.0	4.7706943497549965E-5	1.144966643941199E-4
10	0.003854721034602037	1.3675990469297656E-4	0.0	5.0887406397386625E-5	1.144966643941199E-4
11	0.004532159632267246	1.8446684819052651E-4	0.0	6.042879509689662E-5	1.3675990469297656E-4
12	0.005362260449124616	2.1627147718889317E-4	0.0	7.633110959607994E-5	1.6220360789166987E-4
13	0.006453159223768591	2.9578304968480974E-4	0.0	7.95115724959166E-5	1.685645336913432E-4
14	0.007470907351716324	3.212267528835031E-4	0.0	8.269203539575326E-5	1.7174499659117987E-4
15	0.00866676140205491	3.212267528835031E-4	0.0	8.269203539575326E-5	1.7492545949101653E-4
16	0.009779923416997743	3.275876786831764E-4	0.0	8.587249829558994E-5	1.781059223908532E-4
17	0.01111571783492914	3.275876786831764E-4	0.0	9.223342409526326E-5	1.9082777399019986E-4
18	0.012410166235162664	3.371290673826864E-4	0.0	9.541388699509993E-5	1.9400823689003652E-4
19	0.013555132879103862	3.371290673826864E-4	0.0	9.859434989493659E-5	2.1627147718889317E-4
20	0.014684197208545878	3.371290673826864E-4	0.0	9.859434989493659E-5	2.2581286588840315E-4
21	0.015832344315386913	3.466704560821964E-4	0.0	9.859434989493659E-5	2.385347174877498E-4
22	0.016872355683633503	3.4985091898203307E-4	0.0	1.0177481279477325E-4	2.385347174877498E-4
23	0.018131818991968824	3.5303138188186973E-4	0.0	1.0813573859444658E-4	2.480761061872598E-4
24	0.019238620081111983	3.562118447817064E-4	0.0	1.1131620149428324E-4	2.5125656908709645E-4
25	0.02050762477814681	3.625727705813797E-4	0.0	1.1767712729395658E-4	2.6397842068644315E-4
26	0.021639869570488662	3.721141592808897E-4	0.0	1.2085759019379324E-4	2.830611980854631E-4
27	0.022857986861126105	4.0391878827925634E-4	0.0	1.240380530936299E-4	2.926025867849731E-4
28	0.02398069026476845	4.7388897207566297E-4	0.0	1.3039897889330324E-4	3.212267528835031E-4
29	0.025249694961803276	5.025131381741929E-4	0.0	1.335794417931399E-4	3.403095302825231E-4
30	0.026461451326641044	5.311373042727229E-4	0.0	1.335794417931399E-4	3.4348999318235975E-4
31	0.027711373246276854	5.311373042727229E-4	0.0	1.3994036759281322E-4	3.466704560821964E-4
32	0.028942212388513643	5.406786929722329E-4	0.0	1.4630129339248654E-4	3.6893369638105303E-4
33	0.03011898366145321	5.502200816717429E-4	0.0	1.4630129339248654E-4	3.78475085080563E-4
34	0.03137526650688869	5.693028590707629E-4	0.0	1.494817562923232E-4	3.8801647378007304E-4
35	0.032746046016718294	6.170098025683129E-4	0.0	1.590231449918332E-4	3.9437739957974636E-4
36	0.03404367487985165	6.170098025683129E-4	0.0	1.590231449918332E-4	3.97557862479583E-4
37	0.03532540142848583	6.297316541676595E-4	0.0	1.6538407079150653E-4	4.134601769787663E-4
38	0.036883828249405795	6.297316541676595E-4	0.0	1.685645336913432E-4	4.3254295437778633E-4
39	0.038295953776933275	6.360925799673328E-4	0.0	1.7174499659117987E-4	4.420843430772963E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	2615	0.0	14.025217	42
TCGTATG	2825	0.0	13.620838	43
CGTATGC	3125	0.0	12.457212	44
TCTCGTA	3095	0.0	12.358941	41
ATCTCGT	3840	0.0	9.902425	40
GATCTCG	4340	0.0	9.072655	39
GATCGGA	4295	0.0	8.804112	1
ACACGTC	4735	0.0	8.36231	13
CGTCTGA	5030	0.0	8.229729	16
GTATGCC	5130	0.0	7.939369	45
ACGTCTG	5380	0.0	7.6943374	15
CCGGAAT	3880	0.0	7.4833746	1
CCCGGAT	4670	0.0	7.1813955	1
ATTACGG	2185	0.0	7.105322	39
CACGTCT	5800	0.0	7.0595503	14
AATTACG	2265	0.0	7.053554	38
CGTCGAT	1090	0.0	7.020886	1
CTCGTTT	4145	0.0	6.9506416	1
CCCCCGT	3225	0.0	6.8396807	1
TTACGGC	2275	0.0	6.824232	40
>>END_MODULE