FastQCFastQC Report
Fri 27 May 2016
SRR2140411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32813884
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA25350.07.36978531
CGCGTAT6601.6871127E-87.16192961
CCCGGTT40250.06.598891
CTCCGAT48650.06.56992241
ATTACGG22700.06.54194139
CCCGGAT48600.06.53036641
CCCCCGT33050.06.47003841
TCGTATG25300.06.40394443
GTCGGTT35550.06.39494041
CTCGTAT21850.06.384654542
TTACGGC24000.06.187623540
CCGGAAT41200.06.11893031
CGCCCAT35600.06.00659471
CGTCGGT17400.05.9506351
CGGGCAT52600.05.94817541
CGCCTGT54350.05.8808961
CCCGAAT34650.05.84647371
CGCGCAT13103.6379788E-125.8420111
CGGGGAT49450.05.7808561
CGGGTTT38400.05.7444651
Produced by FastQC (version 0.11.5)