##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32813884
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76693731226697	34.0	31.0	34.0	31.0	34.0
2	32.98626532598214	34.0	31.0	34.0	31.0	34.0
3	33.066983140429215	34.0	33.0	34.0	31.0	34.0
4	36.4102255923133	37.0	37.0	37.0	35.0	37.0
5	36.29340190268241	37.0	37.0	37.0	35.0	37.0
6	36.254326583223126	37.0	37.0	37.0	35.0	37.0
7	36.27463146392545	37.0	37.0	37.0	35.0	37.0
8	36.253230065663665	37.0	37.0	37.0	35.0	37.0
9	38.04473402173299	39.0	38.0	39.0	37.0	39.0
10	37.9899789064897	39.0	38.0	39.0	35.0	39.0
11	38.04690407877349	39.0	38.0	39.0	37.0	39.0
12	38.024265947913996	39.0	38.0	39.0	35.0	39.0
13	37.990299106317316	39.0	38.0	39.0	35.0	39.0
14	39.515881935829356	41.0	40.0	41.0	37.0	41.0
15	39.50467737985543	41.0	40.0	41.0	37.0	41.0
16	39.48503855867839	41.0	40.0	41.0	37.0	41.0
17	39.46446559023613	41.0	40.0	41.0	37.0	41.0
18	39.45456432405259	41.0	39.0	41.0	36.0	41.0
19	39.46247256191921	41.0	40.0	41.0	37.0	41.0
20	39.451035939543154	41.0	39.0	41.0	37.0	41.0
21	39.413012278583054	41.0	39.0	41.0	36.0	41.0
22	39.38230673942774	41.0	39.0	41.0	36.0	41.0
23	39.33982152188994	41.0	39.0	41.0	36.0	41.0
24	39.32793341379521	41.0	39.0	41.0	36.0	41.0
25	39.28853140335353	41.0	39.0	41.0	36.0	41.0
26	39.23108678631277	41.0	39.0	41.0	36.0	41.0
27	39.16625742932473	41.0	39.0	41.0	36.0	41.0
28	39.11668009187818	40.0	39.0	41.0	36.0	41.0
29	39.07726199068662	40.0	39.0	41.0	36.0	41.0
30	39.01163842719746	40.0	39.0	41.0	35.0	41.0
31	38.97053314383631	40.0	39.0	41.0	35.0	41.0
32	38.91261168595586	40.0	38.0	41.0	35.0	41.0
33	38.857073822775746	40.0	38.0	41.0	35.0	41.0
34	38.770510220612714	40.0	38.0	41.0	35.0	41.0
35	38.73993246273437	40.0	38.0	41.0	35.0	41.0
36	38.6670392934893	40.0	38.0	41.0	35.0	41.0
37	38.6071756394336	40.0	38.0	41.0	35.0	41.0
38	38.53876462780206	40.0	38.0	41.0	34.0	41.0
39	38.47256709995074	40.0	38.0	41.0	34.0	41.0
40	38.40896374839382	40.0	38.0	41.0	34.0	41.0
41	38.32284907205742	40.0	38.0	41.0	34.0	41.0
42	38.23367447145239	40.0	38.0	41.0	34.0	41.0
43	38.15332110639508	40.0	38.0	41.0	34.0	41.0
44	38.07931517646616	40.0	38.0	41.0	33.0	41.0
45	37.99111781464212	40.0	37.0	41.0	33.0	41.0
46	37.883889423147835	40.0	37.0	41.0	33.0	41.0
47	38.08667574981371	40.0	38.0	41.0	33.0	41.0
48	38.091884855812864	40.0	37.0	41.0	33.0	41.0
49	38.08267092063835	40.0	37.0	41.0	33.0	41.0
50	38.01216823342217	40.0	37.0	41.0	33.0	41.0
51	37.94144792490886	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1570.0
3	72.0
4	165.0
5	273.0
6	310.0
7	375.0
8	517.0
9	624.0
10	865.0
11	1075.0
12	1579.0
13	2090.0
14	2836.0
15	3814.0
16	4940.0
17	6002.0
18	6938.0
19	8519.0
20	9615.0
21	11103.0
22	13966.0
23	19072.0
24	28294.0
25	44096.0
26	66391.0
27	96748.0
28	138537.0
29	189161.0
30	252957.0
31	331186.0
32	433580.0
33	572433.0
34	801320.0
35	1144656.0
36	1739275.0
37	2934301.0
38	6383437.0
39	1.7541155E7
40	20037.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.50646832956201	12.83617583709292	13.140720446507284	41.516635386837784
2	23.41752350925602	19.368094310323034	30.462468264957604	26.751913915463344
3	26.089240152125846	20.38381375395854	22.54128161116191	30.985664482753705
4	28.215803408093965	25.345042970225652	19.75326358805925	26.685890033621135
5	29.295709096795736	27.554939854117848	21.56294268608983	21.58640836299659
6	25.68722739435539	32.169032474180746	20.928747721543722	21.214992409920143
7	22.237891741191014	21.071251425158934	35.14347463409086	21.547382199559188
8	22.926822682740024	23.926362999271895	27.62976184105484	25.517052476933237
9	22.577574175614203	22.20160527171974	30.299829791560185	24.92099076110588
10	23.313354188733037	33.157684107129775	24.1462516293408	19.38271007479639
11	27.67295697150633	25.985064736621855	20.595239502888475	25.746738788983347
12	24.78082257155871	23.64150404161953	25.65810521455194	25.919568172269823
13	24.09121090328716	25.369953157632906	26.77232600688172	23.76650993219821
14	24.378046804822006	25.35871401264172	26.280104482602546	23.98313469993372
15	24.577986562029658	25.423077012157414	25.6469243323954	24.352012093417528
16	25.003970880131103	25.19916264712827	25.448526605384476	24.34833986735615
17	25.077835955048783	25.540752810609067	25.305809577433745	24.0756016569084
18	24.67989769208668	25.797043714788533	25.398041877639354	24.125016715485433
19	24.937849478592657	25.345783510418947	25.30741865242164	24.408948358566757
20	24.88154099648795	25.560271987308784	25.363044496652698	24.195142519550565
21	24.571409084858423	25.55410210238324	25.621235426257144	24.253253386501193
22	24.776295911815865	25.47770632699256	25.344143960525976	24.401853800665595
23	24.83241179004508	25.574810943567172	25.44352702376742	24.149250242620326
24	24.526035933574448	25.744249079962504	25.57046286510464	24.159252121358403
25	24.890979664561957	25.438547596274212	25.32270906135587	24.34776367780796
26	24.776926568483777	25.739896150285436	25.35286271805487	24.130314563175915
27	24.51028030165349	25.750608207509007	25.501891010811423	24.237220480026078
28	24.86016386982215	25.382327868262877	25.318911594233377	24.438596667681594
29	24.80385228017105	25.50260943601639	25.345184114906434	24.348354168906123
30	24.54849994335648	25.619807658295322	25.470446757819097	24.3612456405291
31	24.735345005081534	25.39856571078674	25.252336671897535	24.613752612234187
32	24.714184062745108	25.68597287803846	25.312220608467978	24.28762245074846
33	24.492173232677633	25.66318628830241	25.45361627591144	24.39102420310852
34	24.812341148970106	25.473870333245742	25.191898230986897	24.521890286797255
35	24.81666364341641	25.641020483495737	25.23799867172037	24.304317201367486
36	24.56170726758816	25.724730692665492	25.396393826129486	24.317168213616853
37	24.78950729379984	25.398831934986354	25.272279823786548	24.53938094742726
38	24.759747283379845	25.717274492708047	25.284493333764495	24.23848489014761
39	24.502558841779706	25.684599892965075	25.51600769481149	24.29683357044373
40	24.733669841909155	25.407581963166344	25.373126140857693	24.48562205406681
41	24.804882789489053	25.53728353739239	25.329679227677715	24.328154445440838
42	24.507988704288703	25.655532250979547	25.559711869576024	24.276767175155726
43	24.767586905742572	25.364940406824672	25.35411805663262	24.51335463080014
44	24.78736700419116	25.53530195939418	25.40784345228242	24.269487584132246
45	24.555420347681483	25.53219929180791	25.534381363837078	24.37799899667353
46	24.774135022346954	25.353800884077067	25.286539980892663	24.585524112683316
47	24.739594095324684	25.64824902515025	25.34049797764468	24.271658901880382
48	24.45650593749042	25.652065176558807	25.580298036607335	24.31113084934344
49	24.74807958672732	25.309162929697592	25.381209860514193	24.561547623060896
50	24.753970658097003	25.586000069063008	25.357089422787176	24.302939850052812
51	24.457395860964613	25.55552887240981	25.624973790920297	24.36210147570528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	207.5
2	223.0
3	287.5
4	352.0
5	565.5
6	779.0
7	1186.0
8	1593.0
9	2465.5
10	3338.0
11	5064.0
12	6790.0
13	9760.0
14	12730.0
15	18301.0
16	23872.0
17	33264.5
18	42657.0
19	58491.5
20	74326.0
21	99109.0
22	123892.0
23	158349.5
24	192807.0
25	234895.0
26	331261.5
27	385540.0
28	450336.0
29	515132.0
30	585938.5
31	656745.0
32	742633.5
33	828522.0
34	913701.0
35	998880.0
36	1090491.5
37	1182103.0
38	1286264.0
39	1390425.0
40	1487046.5
41	1583668.0
42	1662279.5
43	1740891.0
44	1815661.0
45	1890431.0
46	1974363.0
47	2058295.0
48	2124999.0
49	2191703.0
50	2240890.0
51	2290077.0
52	2296493.0
53	2302909.0
54	2268608.0
55	2234307.0
56	2154188.5
57	2074070.0
58	1957726.0
59	1841382.0
60	1694580.5
61	1547779.0
62	1389219.5
63	1230660.0
64	1077601.0
65	924542.0
66	792013.0
67	659484.0
68	558219.0
69	456954.0
70	385469.0
71	313984.0
72	266358.5
73	218733.0
74	188925.0
75	137491.0
76	115865.0
77	102142.5
78	88420.0
79	76381.0
80	64342.0
81	54420.0
82	44498.0
83	36915.0
84	29332.0
85	23768.5
86	18205.0
87	13946.5
88	9688.0
89	7037.5
90	4387.0
91	2931.5
92	1476.0
93	1025.5
94	575.0
95	363.5
96	152.0
97	97.0
98	42.0
99	38.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04994836941582411
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.767544494275655E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.3160927856025825E-4
22	0.0
23	8.197749464830192E-4
24	1.2189962029487273E-5
25	0.002953018301643292
26	0.0021271483741455294
27	0.009523407835536932
28	0.011257429934231499
29	0.00844459619592731
30	0.010431560006733735
31	0.0029743507351948947
32	0.004595615685116702
33	0.0088133425473193
34	0.007563871439296854
35	0.004619995609175677
36	0.008426311252883078
37	0.016045037521312623
38	0.010778973924574123
39	0.0065947694579526145
40	0.0025964619122807895
41	5.4854829132692734E-5
42	0.007597393834877944
43	0.006573437024401012
44	2.0722935450128365E-4
45	0.003154152675129832
46	0.004144587090025673
47	0.0050222643561487566
48	0.006734954021291719
49	0.018595787075982837
50	0.009950056506568989
51	0.0025233221401038657
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.2813884E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.02863710865107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89894644057999	77.33883839786714
2	7.377151894408539	12.692926464389398
3	1.629241807594883	4.204843566834686
4	0.5413269148360345	1.862784668542995
5	0.2260360258283185	0.9722785619733048
6	0.11403371462740841	0.5886099032299676
7	0.05978354374839499	0.36001677531398873
8	0.03738269271938444	0.25727856848801095
9	0.02304746644731772	0.17844679145430967
>10	0.08895034337501526	1.2497380939814562
>50	0.003484431290337506	0.20093199843961534
>100	5.821396456731349E-4	0.07142325201537746
>500	2.6198670010394993E-5	0.01563080201181843
>1k	6.386228642604092E-6	0.0062521554578893026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.313977217692365E-5	9.142471522115456E-6	0.0	9.142471522115456E-6	0.0
2	1.1275714877275729E-4	6.094981014743637E-5	0.0	2.7427414566346367E-5	3.0474905073718187E-6
3	2.6817916464872E-4	7.009228166955182E-5	0.0	3.352239558109E-5	1.2189962029487275E-5
4	7.192077597397492E-4	7.923475319166729E-5	0.0	3.961737659583364E-5	3.961737659583364E-5
5	9.965293959105846E-4	8.837722471378274E-5	3.0474905073718187E-6	4.87598481179491E-5	4.87598481179491E-5
6	0.0015176502726711658	9.142471522115456E-5	3.0474905073718187E-6	5.180733862532091E-5	6.094981014743637E-5
7	0.001898586586092643	1.0666216775801365E-4	3.0474905073718187E-6	5.7902319640064555E-5	7.009228166955182E-5
8	0.0025080846875670066	1.4323205384647548E-4	3.0474905073718187E-6	6.094981014743637E-5	7.009228166955182E-5
9	0.0032364349188288713	1.5237452536859093E-4	3.0474905073718187E-6	6.704479116218E-5	9.142471522115456E-5
10	0.004260391729305802	1.7675444942756547E-4	3.0474905073718187E-6	6.704479116218E-5	1.0056718674327002E-4
11	0.0050862616568035654	2.2551429754551458E-4	3.0474905073718187E-6	7.009228166955182E-5	1.0970965826538547E-4
12	0.006119360938802612	2.498942216044891E-4	3.0474905073718187E-6	7.313977217692365E-5	1.2494711080224456E-4
13	0.007466351743060955	2.895115982003228E-4	3.0474905073718187E-6	7.313977217692365E-5	1.2494711080224456E-4
14	0.008773725170723466	3.1693901276666915E-4	3.0474905073718187E-6	7.923475319166729E-5	1.310420918169882E-4
15	0.010270043009843029	3.2303399378141276E-4	3.0474905073718187E-6	8.532973420641091E-5	1.310420918169882E-4
16	0.01172979096287413	3.3217646530352824E-4	3.0474905073718187E-6	9.75196962358982E-5	1.3408958232436E-4
17	0.013253536216560039	3.443664273330155E-4	3.0474905073718187E-6	1.0970965826538547E-4	1.493270348612191E-4
18	0.014841278770900756	3.443664273330155E-4	3.0474905073718187E-6	1.2189962029487274E-4	1.5237452536859093E-4
19	0.016291884252409742	3.5046140834775913E-4	3.0474905073718187E-6	1.2494711080224456E-4	1.7065946841282183E-4
20	0.017736394752903985	3.5350889885513094E-4	3.0474905073718187E-6	1.310420918169882E-4	1.7065946841282183E-4
21	0.019208332667964574	3.717938418993619E-4	3.0474905073718187E-6	1.3408958232436E-4	1.7370695892019366E-4
22	0.020619320772877724	3.717938418993619E-4	3.0474905073718187E-6	1.493270348612191E-4	1.828494304423091E-4
23	0.02206992625438671	3.839838039288491E-4	3.0474905073718187E-6	1.5542201587596274E-4	1.828494304423091E-4
24	0.02352662671691044	3.931262754509646E-4	3.0474905073718187E-6	1.6456448739807821E-4	1.8589692094968095E-4
25	0.0249802796889268	4.022687469730801E-4	3.0474905073718187E-6	1.7065946841282183E-4	1.8894441145705275E-4
26	0.026564974752760144	4.114112184951955E-4	3.0474905073718187E-6	1.7065946841282183E-4	2.1027684500865549E-4
27	0.02811919491151977	4.3579114255417006E-4	3.0474905073718187E-6	1.828494304423091E-4	2.2551429754551458E-4
28	0.029856264500721708	4.8455099067211914E-4	3.0474905073718187E-6	1.8894441145705275E-4	2.559892026192328E-4
29	0.031596381580431016	4.997884432089782E-4	3.0474905073718187E-6	1.8894441145705275E-4	2.651316741413482E-4
30	0.03337611603673616	5.333108387900683E-4	3.0474905073718187E-6	1.9199190196442456E-4	2.712266551560919E-4
31	0.035201562850651874	5.455008008195555E-4	3.0474905073718187E-6	1.9199190196442456E-4	2.773216361708355E-4
32	0.03713367183232561	5.54643272341671E-4	3.0474905073718187E-6	1.980868829791682E-4	2.925590887076946E-4
33	0.03899568853232979	5.607382533564146E-4	3.0474905073718187E-6	1.980868829791682E-4	3.1998650327404095E-4
34	0.04075409055508333	5.820706869080173E-4	3.0474905073718187E-6	1.980868829791682E-4	3.2912897479615643E-4
35	0.04249420763479264	6.064506109669919E-4	3.0474905073718187E-6	2.0113437348654004E-4	3.443664273330155E-4
36	0.04433184441073784	6.247355540112228E-4	3.0474905073718187E-6	2.0722935450128365E-4	3.5350889885513094E-4
37	0.04615119624363882	6.308305350259664E-4	3.0474905073718187E-6	2.1637182602339913E-4	3.626513703772464E-4
38	0.04805587781074621	6.308305350259664E-4	3.0474905073718187E-6	2.1941931653077094E-4	3.748413324067337E-4
39	0.05001846169749366	6.308305350259664E-4	3.0474905073718187E-6	2.3160927856025822E-4	3.900787849435928E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTA	2535	0.0	7.3697853	1
CGCGTAT	660	1.6871127E-8	7.1619296	1
CCCGGTT	4025	0.0	6.59889	1
CTCCGAT	4865	0.0	6.5699224	1
ATTACGG	2270	0.0	6.541941	39
CCCGGAT	4860	0.0	6.5303664	1
CCCCCGT	3305	0.0	6.4700384	1
TCGTATG	2530	0.0	6.403944	43
GTCGGTT	3555	0.0	6.3949404	1
CTCGTAT	2185	0.0	6.3846545	42
TTACGGC	2400	0.0	6.1876235	40
CCGGAAT	4120	0.0	6.1189303	1
CGCCCAT	3560	0.0	6.0065947	1
CGTCGGT	1740	0.0	5.950635	1
CGGGCAT	5260	0.0	5.9481754	1
CGCCTGT	5435	0.0	5.880896	1
CCCGAAT	3465	0.0	5.8464737	1
CGCGCAT	1310	3.6379788E-12	5.842011	1
CGGGGAT	4945	0.0	5.780856	1
CGGGTTT	3840	0.0	5.744465	1
>>END_MODULE