##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32102149
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74675904096016	34.0	31.0	34.0	31.0	34.0
2	32.96710104983937	34.0	31.0	34.0	31.0	34.0
3	33.04734184617983	34.0	33.0	34.0	31.0	34.0
4	36.39642791515297	37.0	37.0	37.0	35.0	37.0
5	36.277439993191734	37.0	37.0	37.0	35.0	37.0
6	36.23810418424013	37.0	37.0	37.0	35.0	37.0
7	36.25866221604043	37.0	37.0	37.0	35.0	37.0
8	36.23791170491421	37.0	37.0	37.0	35.0	37.0
9	38.026142922705894	39.0	38.0	39.0	35.0	39.0
10	37.96948730753196	39.0	38.0	39.0	35.0	39.0
11	38.025955863577856	39.0	38.0	39.0	35.0	39.0
12	38.003566957464436	39.0	38.0	39.0	35.0	39.0
13	37.96943983407466	39.0	38.0	39.0	35.0	39.0
14	39.48794250503292	41.0	40.0	41.0	37.0	41.0
15	39.47774969831459	41.0	40.0	41.0	37.0	41.0
16	39.45856182400748	41.0	39.0	41.0	37.0	41.0
17	39.43891419854789	41.0	39.0	41.0	36.0	41.0
18	39.42891773382524	41.0	39.0	41.0	36.0	41.0
19	39.4389158495277	41.0	39.0	41.0	37.0	41.0
20	39.42799003892232	41.0	39.0	41.0	36.0	41.0
21	39.390571173288116	41.0	39.0	41.0	36.0	41.0
22	39.36075927502548	41.0	39.0	41.0	36.0	41.0
23	39.31850562403159	41.0	39.0	41.0	36.0	41.0
24	39.30823276036754	41.0	39.0	41.0	36.0	41.0
25	39.26867410029154	41.0	39.0	41.0	36.0	41.0
26	39.211588202397294	41.0	39.0	41.0	36.0	41.0
27	39.147638683005304	40.0	39.0	41.0	36.0	41.0
28	39.09830921288167	40.0	39.0	41.0	36.0	41.0
29	39.059822132156945	40.0	39.0	41.0	36.0	41.0
30	38.99671174661858	40.0	39.0	41.0	35.0	41.0
31	38.956149135062574	40.0	39.0	41.0	35.0	41.0
32	38.89867351248043	40.0	38.0	41.0	35.0	41.0
33	38.846271786976004	40.0	38.0	41.0	35.0	41.0
34	38.761624743564674	40.0	38.0	41.0	35.0	41.0
35	38.733173564174784	40.0	38.0	41.0	35.0	41.0
36	38.66138939794965	40.0	38.0	41.0	35.0	41.0
37	38.60333808182125	40.0	38.0	41.0	35.0	41.0
38	38.53503832406983	40.0	38.0	41.0	34.0	41.0
39	38.47197818438884	40.0	38.0	41.0	34.0	41.0
40	38.411029959396174	40.0	38.0	41.0	34.0	41.0
41	38.326533747008654	40.0	38.0	41.0	34.0	41.0
42	38.24055610731855	40.0	38.0	41.0	34.0	41.0
43	38.16215727489147	40.0	38.0	41.0	34.0	41.0
44	38.08963702087359	40.0	38.0	41.0	33.0	41.0
45	38.00533425347942	40.0	38.0	41.0	33.0	41.0
46	37.90026932464864	40.0	37.0	41.0	33.0	41.0
47	38.106460069075126	40.0	38.0	41.0	33.0	41.0
48	38.11384957436962	40.0	38.0	41.0	33.0	41.0
49	38.10813811872844	40.0	37.0	41.0	33.0	41.0
50	38.04185778964517	40.0	37.0	41.0	33.0	41.0
51	37.97527227850073	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1204.0
3	53.0
4	121.0
5	206.0
6	254.0
7	313.0
8	451.0
9	566.0
10	745.0
11	881.0
12	1295.0
13	1868.0
14	2596.0
15	3430.0
16	4375.0
17	5500.0
18	6377.0
19	7962.0
20	8729.0
21	10496.0
22	13383.0
23	18439.0
24	27815.0
25	42517.0
26	65628.0
27	95805.0
28	137511.0
29	189367.0
30	251817.0
31	332786.0
32	432210.0
33	570381.0
34	795359.0
35	1127410.0
36	1704480.0
37	2868104.0
38	6251743.0
39	1.7100738E7
40	19234.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.08416943212432	13.16534442865568	13.412864367562763	41.33762177165724
2	23.265264889275795	19.617375148311723	30.547123184806104	26.570236777606382
3	25.818389915267044	20.60584791379543	22.863618881091107	30.712143289846423
4	27.853988840435573	25.596529378765265	20.03259968670633	26.516882094092832
5	29.00251631129118	27.915832052240493	21.754070109138176	21.327581527330146
6	25.403293094178835	32.545712126624295	21.0698978439107	20.98109693528617
7	21.90219414905837	21.276469684319267	35.5239644548407	21.29737171178166
8	22.632438096278225	24.211432075777857	27.890419423322722	25.2657104046212
9	22.31952758053674	22.413122560735733	30.56751122798664	24.69983863074089
10	22.974256334054147	33.54128410531021	24.30593042229042	19.178529138345223
11	27.386543499003757	26.31803247813721	20.777496858543646	25.517927164315385
12	24.500561832893528	23.919300286909827	25.899297092100472	25.68084078809617
13	23.76323466693772	25.67512536310264	27.058300676381506	23.503339293578136
14	24.075609393003564	25.666013823560537	26.52447660123938	23.733900182196525
15	24.261911562369235	25.734946903398896	25.893553107612828	24.10958842661904
16	24.694309405890554	25.472441112898704	25.732576345589823	24.10067313562092
17	24.759962331493757	25.835342051399735	25.565385046340666	23.83931057076584
18	24.383193162551205	26.112865528099068	25.639364517310042	23.864576792039685
19	24.610470781878185	25.67960169893922	25.570010904877428	24.139916614305168
20	24.571143196675088	25.860583352223554	25.603500874661073	23.96477257644029
21	24.272750598878474	25.86446632803242	25.862111341103066	24.00067173198604
22	24.47592215711166	25.779523358389493	25.61692365205831	24.127630832440534
23	24.524954642846172	25.858717566776928	25.714997021046926	23.901330769329974
24	24.224273750197924	26.046360830178845	25.82170916128559	23.907656258337646
25	24.572272526813073	25.75748905267566	25.588171516082113	24.082066904429155
26	24.45553045463268	26.061410799239415	25.598722428096863	23.884336318031046
27	24.211367196138994	26.045908758261227	25.74579216030709	23.996931885292685
28	24.521672400951687	25.703389861398605	25.599260225984118	24.175677511665594
29	24.48957148728209	25.806768271361896	25.602664157453383	24.10099608390263
30	24.234281296140747	25.934086379184667	25.720807364811606	24.110824959862985
31	24.3937486003233	25.731606671072942	25.53818065719301	24.336464071410745
32	24.3864388762192	26.001408132471244	25.58658193864035	24.025571052669207
33	24.200299295463115	25.95887198333775	25.72708560388573	24.113743117313405
34	24.477861098746853	25.804854974327483	25.475291105560625	24.241992821365038
35	24.491000610360956	25.940399948574296	25.514025763381877	24.05457367768287
36	24.234686256139792	26.022419659522168	25.676615871247755	24.066278213090285
37	24.486849937198173	25.72145460969166	25.545628181080502	24.24606727202967
38	24.46851589201333	26.0042851576989	25.548581372542728	23.97861757774504
39	24.188713754139595	25.97169879356867	25.784723977801278	24.054863474490457
40	24.416871219834256	25.72337089082859	25.649230524236877	24.21052736510028
41	24.49579431294381	25.83818822970827	25.60951490846697	24.05650254888095
42	24.199789730043484	25.953616745433788	25.83322928247776	24.013364242044968
43	24.417639392543663	25.69277308767251	25.658499035127164	24.23108848465666
44	24.48423356007566	25.823950529808364	25.685143320468434	24.006672589647543
45	24.25247457653307	25.840367894619597	25.80565262632811	24.10150490251922
46	24.457753336882774	25.675568707027413	25.565484370612946	24.30119358547687
47	24.422554967907313	25.957931815178465	25.613404020590608	24.006109196323617
48	24.153953271028037	25.97186292834891	25.842803738317755	24.031380062305296
49	24.43437223680693	25.61468368975343	25.665316135770105	24.28562793766953
50	24.431007617469636	25.888225022904248	25.61146119636443	24.069306163261682
51	24.13563174241819	25.86967800560319	25.886998157553258	24.107692094425357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	202.0
1	219.5
2	237.0
3	294.0
4	351.0
5	597.5
6	844.0
7	1285.0
8	1726.0
9	2671.0
10	3616.0
11	5482.5
12	7349.0
13	10477.0
14	13605.0
15	19660.5
16	25716.0
17	35666.5
18	45617.0
19	62475.0
20	79333.0
21	105116.0
22	130899.0
23	166513.0
24	202127.0
25	247072.0
26	348393.0
27	404769.0
28	472528.0
29	540287.0
30	614247.5
31	688208.0
32	776015.0
33	863822.0
34	950529.5
35	1037237.0
36	1128132.0
37	1219027.0
38	1323622.0
39	1428217.0
40	1522603.5
41	1616990.0
42	1690920.5
43	1764851.0
44	1834881.0
45	1904911.0
46	1979335.5
47	2053760.0
48	2108932.5
49	2164105.0
50	2201498.5
51	2238892.0
52	2233572.0
53	2228252.0
54	2185262.5
55	2142273.0
56	2054121.5
57	1965970.0
58	1845450.5
59	1724931.0
60	1582437.0
61	1439943.0
62	1285325.5
63	1130708.0
64	986639.0
65	842570.0
66	718098.5
67	593627.0
68	499313.0
69	404999.0
70	339674.0
71	274349.0
72	231203.0
73	188057.0
74	161212.0
75	114818.5
76	95270.0
77	83565.5
78	71861.0
79	61642.0
80	51423.0
81	43554.0
82	35685.0
83	29264.0
84	22843.0
85	18420.0
86	13997.0
87	10636.5
88	7276.0
89	5321.0
90	3366.0
91	2259.5
92	1153.0
93	757.0
94	361.0
95	231.5
96	102.0
97	65.0
98	28.0
99	25.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04558261816054745
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	2.3985933153571745E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.8690337522263696E-4
22	0.0
23	8.317200197407344E-4
24	1.8690337522263697E-5
25	0.0028845420909360308
26	0.0019593703835839777
27	0.009214336398476002
28	0.01151947802622186
29	0.008862335041806703
30	0.010438553506184275
31	0.0030714454661586676
32	0.004597823030476869
33	0.008613130541509853
34	0.007641232990352141
35	0.004585362805462027
36	0.008510333685137402
37	0.016054999931624517
38	0.010737598906540493
39	0.006554078357807137
40	0.002735019390757921
41	6.853123758163355E-5
42	0.007666153440381827
43	0.006429476107658712
44	2.180539377597431E-4
45	0.003115056253710616
46	0.0040682634673460645
47	0.005267560125024651
48	0.006694255889224114
49	0.019092179778992364
50	0.010282800693498744
51	0.0025356557905204416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.2102149E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.07919707262333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.81465484553112	77.31173374458118
2	7.457543484987772	12.83878710643841
3	1.6604609928761906	4.287934471115802
4	0.5259092664153175	1.8107938954433154
5	0.222655652091389	0.9583009877854064
6	0.11398103350717813	0.5886837507483401
7	0.056388075904903585	0.3397688208854967
8	0.034262344554291925	0.23594200872472137
9	0.026325950322407667	0.20395049993339587
>10	0.08452908117040366	1.1973196447313457
>50	0.002886883187450247	0.1643970617669408
>100	3.761388807783814E-4	0.04796418306939254
>500	2.6250570813274084E-5	0.014423824776200371
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.230112507421232E-5	6.2301125074212325E-6	0.0	9.345168761131849E-6	9.345168761131849E-6
2	9.345168761131848E-5	5.607101256679109E-5	0.0	2.1805393775974314E-5	1.557528126855308E-5
3	1.900184314763476E-4	6.230112507421232E-5	0.0	2.8035506283395546E-5	2.492045002968493E-5
4	5.389047318919366E-4	6.853123758163355E-5	0.0	4.672584380565924E-5	3.7380675044527395E-5
5	7.569586696516797E-4	7.78764063427654E-5	0.0	4.984090005936986E-5	5.607101256679109E-5
6	0.0011463407013655067	9.65667438650291E-5	0.0	5.607101256679109E-5	6.230112507421232E-5
7	0.0013799699203938028	1.1214202513358218E-4	0.0	5.9186068820501705E-5	7.164629383534417E-5
8	0.0016821303770037327	1.4017753141697773E-4	0.0	5.9186068820501705E-5	7.78764063427654E-5
9	0.0021151231962695085	1.4329258767068834E-4	0.0	6.853123758163355E-5	8.099146259647602E-5
10	0.002735019390757921	1.6198292519295203E-4	0.0	7.164629383534417E-5	9.033663135760786E-5
11	0.0033455704164852018	1.8378831896892635E-4	0.0	7.78764063427654E-5	1.0591191262616095E-4
12	0.004030882792301537	2.460894440431387E-4	0.0	7.78764063427654E-5	1.1214202513358218E-4
13	0.004818992024490323	2.710098940728236E-4	0.0	7.78764063427654E-5	1.3083236265584586E-4
14	0.005737933619334955	2.897002315950873E-4	0.0	8.722157510389725E-5	1.370624751632671E-4
15	0.006734751620522352	2.897002315950873E-4	0.0	8.722157510389725E-5	1.4017753141697773E-4
16	0.007578931865277929	2.9593034410250855E-4	0.0	9.345168761131848E-5	1.4640764392439895E-4
17	0.008485413235107719	3.1462068162477224E-4	0.0	9.968180011873972E-5	1.557528126855308E-4
18	0.009416815054967193	3.1773573787848285E-4	0.0	1.0591191262616095E-4	1.557528126855308E-4
19	0.01031706631228956	3.2396585038590406E-4	0.0	1.0591191262616095E-4	1.8067326271521572E-4
20	0.011239122963387903	3.3019596289332533E-4	0.0	1.1525708138729279E-4	1.962485439837688E-4
21	0.012158064558232535	3.3019596289332533E-4	0.0	1.1525708138729279E-4	1.9936360023747944E-4
22	0.012977324352958427	3.3331101914703593E-4	0.0	1.1837213764100341E-4	2.2116899401345374E-4
23	0.013868230441519663	3.3954113165445715E-4	0.0	1.3083236265584586E-4	2.3051416277458558E-4
24	0.014746676305066056	3.48886300415589E-4	0.0	1.4017753141697773E-4	2.3362921902829621E-4
25	0.015787105093805404	3.6134652543043144E-4	0.0	1.4017753141697773E-4	2.460894440431387E-4
26	0.016877374782604117	3.644615816841421E-4	0.0	1.4640764392439895E-4	2.647797815654024E-4
27	0.01784927233376183	3.862669754601164E-4	0.0	1.4640764392439895E-4	2.8035506283395547E-4
28	0.01894888719132168	4.236476505046438E-4	0.0	1.4952270017810958E-4	2.9904540035621916E-4
29	0.020182449467791082	4.5168315678803934E-4	0.0	1.6509798144666266E-4	3.0527551286364037E-4
30	0.021394206350484512	4.890638318325667E-4	0.0	1.7132809395408388E-4	3.08390569117351E-4
31	0.02254366210810373	4.921788880862774E-4	0.0	1.7132809395408388E-4	3.1773573787848285E-4
32	0.023777224384573133	4.95293944339988E-4	0.0	1.744431502077945E-4	3.1773573787848285E-4
33	0.024970290929744298	4.984090005936986E-4	0.0	1.7755820646150512E-4	3.3331101914703593E-4
34	0.02618516286869144	5.077541693548304E-4	0.0	1.8067326271521572E-4	3.3642607540074654E-4
35	0.027481026270235055	5.638251819216215E-4	0.0	1.8378831896892635E-4	3.3954113165445715E-4
36	0.028786234840539802	5.669402381753321E-4	0.0	1.8690337522263696E-4	3.5511641292301023E-4
37	0.0300478326232926	5.76285406936464E-4	0.0	1.931334877300582E-4	3.5511641292301023E-4
38	0.03138419175613446	5.794004631901746E-4	0.0	2.0247865649119005E-4	3.706916941915633E-4
39	0.03281711763284134	5.825155194438852E-4	0.0	2.118238252523219E-4	3.862669754601164E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	2320	0.0	7.3707023	42
TCGTATG	2510	0.0	7.351492	43
CGCGTAT	620	4.0596206E-8	7.2606897	1
CCCGGAT	4390	0.0	7.177994	1
CGGGCTA	2530	0.0	7.117198	1
CCCCGAT	3210	0.0	6.871643	1
CGCCCAT	3365	0.0	6.7557855	1
TCTCGTA	2775	0.0	6.729731	41
GCCGGTT	2680	0.0	6.634862	1
CTCCGAT	4735	0.0	6.559922	1
CGTATGC	2855	0.0	6.5419316	44
ATTACGG	2275	0.0	6.527484	39
CGCCCGT	1925	0.0	6.4308963	1
CCCCCGT	3085	0.0	6.420474	1
GTCCGAT	1810	0.0	6.3420715	1
CGGGTGT	4020	0.0	6.214934	1
CGGGGAT	4335	0.0	6.1267824	1
CGTCGAT	1140	1.6370905E-11	6.1206336	1
TTACGGC	2210	0.0	6.108579	40
CGCCCTA	1600	0.0	6.0490623	1
>>END_MODULE