FastQCFastQC Report
Fri 27 May 2016
SRR2140409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15692903
Sequences flagged as poor quality0
Sequence length19-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT208900.074.0107594
TATCTCG268850.055.83650694
GGTATCA252850.053.48391
TATGCCG117450.041.74521386-87
GCCGTCT124650.041.70690-91
ATGCCGT125550.040.4406988-89
TGCCGTC127050.039.351888-89
CCGTCTT132150.038.39663390-91
CTCGTAT124150.038.16423882-83
TCTCGTA126000.037.76637682-83
TCGTATG137650.036.09454784-85
CGTATGC139900.034.87453584-85
GTATCAA432550.034.3776361
CTATCTC312050.033.3630892-93
GTATGCC152700.033.1250286-87
ACGCTAT609900.028.4157694
GCTATCT384300.027.83756492-93
CGCTATC345550.027.08578390-91
ATCAACG545750.026.717713
TCAACGC547050.026.6587264
Produced by FastQC (version 0.11.5)