##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15692903
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38960770993104	33.0	31.0	34.0	31.0	34.0
2	32.454315622800955	34.0	31.0	34.0	31.0	34.0
3	32.48620487872766	34.0	31.0	34.0	30.0	34.0
4	36.07784837515404	37.0	35.0	37.0	35.0	37.0
5	35.99258295294376	37.0	35.0	37.0	35.0	37.0
6	36.08599135545539	37.0	35.0	37.0	35.0	37.0
7	36.02534903835192	37.0	35.0	37.0	35.0	37.0
8	36.027846154404955	37.0	35.0	37.0	35.0	37.0
9	37.76741174019874	39.0	38.0	39.0	35.0	39.0
10-11	37.71292472144893	39.0	37.5	39.0	35.0	39.0
12-13	37.696722078763884	39.0	37.0	39.0	35.0	39.0
14-15	39.083886550499926	41.0	38.0	41.0	36.0	41.0
16-17	39.08217412036511	41.0	38.0	41.0	36.0	41.0
18-19	39.122324212416274	41.0	38.5	41.0	36.0	41.0
20-21	39.13216125017355	41.0	39.0	41.0	35.5	41.0
22-23	39.086843392820256	41.0	39.0	41.0	35.0	41.0
24-25	39.04762729204667	41.0	38.5	41.0	35.0	41.0
26-27	38.94898028634695	40.0	38.0	41.0	35.0	41.0
28-29	38.88049425828326	40.0	38.0	41.0	35.0	41.0
30-31	38.79489749816468	40.0	38.0	41.0	35.0	41.0
32-33	38.681498513381634	40.0	38.0	41.0	34.5	41.0
34-35	38.61171036983674	40.0	38.0	41.0	34.0	41.0
36-37	38.56316850927447	40.0	38.0	41.0	34.0	41.0
38-39	38.509156742092344	40.0	38.0	41.0	34.0	41.0
40-41	38.460150975271816	40.0	38.0	41.0	34.0	41.0
42-43	38.384736669399054	40.0	38.0	41.0	34.0	41.0
44-45	38.310452381063186	40.0	38.0	41.0	33.0	41.0
46-47	38.22919120787802	40.0	37.5	41.0	33.0	41.0
48-49	38.1288131407819	40.0	37.0	41.0	33.0	41.0
50-51	38.01618160376123	40.0	37.0	41.0	33.0	41.0
52-53	37.89345188056531	40.0	37.0	41.0	33.0	41.0
54-55	37.722796605307614	40.0	36.0	41.0	33.0	41.0
56-57	37.54953462394843	39.0	36.0	41.0	32.5	41.0
58-59	37.349208888319964	39.0	35.0	41.0	32.0	41.0
60-61	37.130751780118885	39.0	35.0	41.0	32.0	41.0
62-63	36.88668780596089	38.0	35.0	40.5	32.0	41.0
64-65	36.64457970646492	37.5	35.0	40.0	31.0	41.0
66-67	36.35981940258407	37.0	35.0	40.0	31.0	41.0
68-69	36.03578936881911	36.5	35.0	39.0	31.0	41.0
70-71	35.7169523255873	36.0	34.5	39.0	31.0	41.0
72-73	35.40299200915902	35.5	34.0	39.0	31.0	40.5
74-75	35.06453530780501	35.0	34.0	37.5	30.0	40.0
76-77	34.75347585117149	35.0	34.0	37.0	30.0	39.0
78-79	34.44537976939904	35.0	34.0	36.5	30.0	39.0
80-81	34.172493307435815	35.0	34.0	36.0	30.0	38.0
82-83	33.93112708964294	35.0	34.0	35.5	30.0	37.0
84-85	33.765060690392374	35.0	33.0	35.0	30.0	37.0
86-87	33.60424579931227	35.0	33.0	35.0	30.0	36.0
88-89	33.48599136507283	35.0	33.0	35.0	29.0	36.0
90-91	33.42272426854514	35.0	33.0	35.0	29.0	36.0
92-93	33.36974787685678	35.0	33.0	35.0	29.0	35.5
94-95	33.29690516717983	35.0	33.0	35.0	29.0	35.0
96-97	33.30190670499371	35.0	33.0	35.0	29.0	35.0
98-99	33.36494976594135	35.0	33.0	35.0	29.5	35.0
100	33.329764662925854	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	2.0
19	15.0
20	75.0
21	408.0
22	1868.0
23	6512.0
24	17050.0
25	37095.0
26	66903.0
27	106015.0
28	153330.0
29	207408.0
30	266430.0
31	333924.0
32	422942.0
33	544883.0
34	744127.0
35	1067410.0
36	1721015.0
37	3144428.0
38	4129923.0
39	2521784.0
40	199353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.890298882239954	19.28899324745715	12.93209420844569	24.88861366185721
2	16.63129505101765	21.36327485105847	37.74167851544102	24.263751582482858
3	18.292139010106347	25.490566683402992	30.433942823197423	25.78335148329323
4	13.579511344589676	17.842210033977786	36.463900009806984	32.11437861162555
5	13.979058216214446	37.07378210722959	34.60407348718218	14.34308618937378
6	31.343350348958705	36.31506641472148	17.9079160414077	14.433667194912122
7	27.096849016878995	32.109721096446286	22.74051034609326	18.05291954058146
8	25.86206006626053	33.84466851034509	20.982006962000593	19.31126446139379
9	26.625016416656628	14.859500501596168	20.42905637025858	38.08642671148863
10-11	25.027878500817145	25.29454976464156	28.49467822163541	21.182893512905878
12-13	26.00130772489959	23.657404241904764	27.920853777022646	22.420434256172996
14-15	23.44611446333416	24.82002533247035	25.65496645203249	26.078893752163
16-17	22.741120619938833	27.307054023082916	26.676316676398244	23.275508680580003
18-19	22.830998827941524	26.39056967343773	28.371356784656093	22.40707471396465
20-21	23.77200218251811	25.637249660112698	28.07491744501613	22.51583071235306
22-23	23.6141141452175	25.496868073470807	27.8422006185774	23.0468171627343
24-25	23.243206901768804	25.78446738604509	27.984777895253544	22.98754781693256
26-27	23.035858893661803	25.874996942223344	28.11858694136892	22.97055722274593
28-29	22.95572788127694	25.786684853923642	28.257876905508294	22.999710359291125
30-31	23.296737307318764	25.684421967089342	28.006945910268072	23.011894815323828
32-33	23.076983176684376	25.861702153996962	27.871332811804255	23.189981857514404
34-35	23.305813508965937	25.90723488476861	27.855793902644304	22.931157703621146
36-37	23.290754731727777	25.792585745885336	27.765226408157133	23.151433114229754
38-39	23.038699278301337	25.797181258431284	27.838279654954402	23.325839808312978
40-41	23.408318362198532	25.661763175258145	27.96560982969252	22.964308632850802
42-43	23.270318456378146	25.73488327746131	27.488842317275058	23.505955948885486
44-45	23.219735887957043	25.838061506868982	27.26782548380498	23.674377121368998
46-47	23.373232173002034	25.89778482523304	27.255173594266367	23.473809407498557
48-49	23.351574803330777	26.355546577022615	26.7753008582377	23.517577761408905
50-51	23.253643886959242	26.448573555487116	26.388604155211997	23.909178402341645
52-53	23.23873726581805	26.100313057035656	26.367118563639924	24.29383111350637
54-55	23.562307299524697	25.78106842640855	26.881789430426327	23.774834843640424
56-57	23.19746519927732	25.13990611822393	27.157756888646727	24.504871793852022
58-59	22.85896280548326	24.782006730519797	27.319787440045832	25.039243023951112
60-61	23.20053278573661	25.285018196512087	27.794792606302675	23.71965641144863
62-63	22.4122457110817	25.642818936620582	27.790732173232573	24.154203179065146
64-65	22.196554890190427	26.214362421591325	27.122563376824882	24.466519311393363
66-67	22.21618590847713	27.367898827458475	26.309670231938508	24.10624503212589
68-69	22.465354096256924	27.335720177993654	25.92539739713246	24.27352832861696
70-71	22.46815992841445	26.573753350395286	25.93955740364627	25.018529317544
72-73	22.856257575241138	25.57728639457153	25.896102888021066	25.670353142166263
74-75	23.0791081557672	25.565223150011352	26.232396593506202	25.123272100715244
76-77	23.672224684978545	25.474393031649363	25.164474445962533	25.688907837409563
78-79	24.068817786184475	25.515994364519003	24.35881596005036	26.056371889246165
80-81	23.581328880042197	26.23636572679155	24.719502670545005	25.46280272262125
82-83	23.587095812215154	25.864212178416384	24.674947282481202	25.87374472688726
84-85	23.635487980752938	26.630075542389946	24.588738533289575	25.145697943567534
86-87	24.727600735578793	26.41401459595969	24.66949502503109	24.188889643430425
88-89	24.086217875131638	26.59951987950537	25.39092549384324	23.92333675151975
90-91	24.03676847587522	25.821830886674103	25.091299967617747	25.05010066983293
92-93	23.989242849129607	25.107938162396703	25.487216788704504	25.415602199769182
94-95	22.73591955228633	25.79309737149086	26.69418858539972	24.776794490823093
96-97	22.910568835578644	25.527236285999734	26.98941590253956	24.572778975882063
98-99	24.22970602944978	27.31616547680668	27.365371113106878	21.088757380636665
100	32.1283162584467	39.192440003209214	28.67924373834408	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6232.0
1	5854.0
2	6882.0
3	8278.5
4	10186.5
5	16809.5
6	22351.0
7	24943.5
8	25568.5
9	23361.5
10	19788.5
11	15900.5
12	16278.0
13	23144.5
14	41031.0
15	66729.0
16	87680.0
17	99547.5
18	103720.0
19	99446.0
20	87443.5
21	72250.0
22	59895.5
23	56009.0
24	60731.5
25	71138.0
26	87699.5
27	107957.0
28	128829.5
29	152698.5
30	179209.5
31	207607.0
32	240882.0
33	277715.5
34	311380.5
35	345636.0
36	384913.5
37	420256.0
38	451996.0
39	478448.5
40	503526.5
41	529054.0
42	552609.5
43	583939.0
44	617536.5
45	647691.0
46	673255.5
47	694743.5
48	714135.0
49	726944.0
50	732838.5
51	734063.5
52	727380.5
53	710555.0
54	687068.5
55	655355.5
56	616881.5
57	574157.0
58	526270.0
59	470387.0
60	407566.0
61	340206.0
62	279060.5
63	226721.0
64	179498.0
65	140614.0
66	108837.0
67	86078.5
68	68145.0
69	53633.0
70	42418.0
71	33701.0
72	26673.5
73	20742.0
74	15483.0
75	11380.0
76	8303.0
77	5716.0
78	4006.0
79	2835.5
80	2011.0
81	1450.0
82	1067.0
83	785.0
84	576.5
85	408.5
86	288.0
87	211.5
88	133.0
89	84.0
90	60.5
91	44.5
92	33.0
93	19.0
94	9.5
95	7.5
96	6.0
97	4.5
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003122430566224745
4	2.5489229112038734E-5
5	0.11300649726822373
6	4.588061240166972E-4
7	1.4656306739422274E-4
8	0.0
9	0.0
10-11	0.013053671459002837
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.603121104000932E-5
22-23	1.2747600630674794E-5
24-25	4.207942491452617E-4
26-27	0.0
28-29	3.1288250285058306E-4
30-31	0.0
32-33	9.324110651857578E-5
34-35	9.481098814697619E-4
36-37	0.0010936668170485745
38-39	2.799852787034402E-4
40-41	0.002344294066385553
42-43	0.00141737359998586
44-45	0.002085890258646289
46-47	5.919140295707018E-4
48-49	0.00542977462577119
50-51	0.0019961875689645104
52-53	4.219419934342941E-4
54-55	5.055597123798574E-4
56-57	6.508013823599852E-5
58-59	0.00392034765773345
60-61	0.0026635650281229294
62-63	0.002796572737058034
64-65	6.771565234513224E-4
66-67	9.476629809327534E-4
68-69	0.004667230720784281
70-71	0.0026231673724367516
72-73	0.0
74-75	0.001399332071141229
76-77	0.0013356598902120652
78-79	1.472347381097853E-4
80-81	8.560947525672142E-6
82-83	4.3547484329328896E-5
84-85	7.530004409924936E-5
86-87	0.0011498359319399938
88-89	1.1945796042348582E-4
90-91	0.0014195144445432952
92-93	0.001816504202673684
94-95	5.568109580396543E-4
96-97	1.6059830094023781E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	548.0
20-21	2203.0
22-23	4354.0
24-25	6229.0
26-27	12706.0
28-29	28650.0
30-31	66794.0
32-33	91120.0
34-35	125446.0
36-37	168871.0
38-39	103515.0
40-41	181863.0
42-43	214574.0
44-45	261388.0
46-47	245388.0
48-49	206552.0
50-51	103268.0
52-53	19114.0
54-55	16996.0
56-57	16473.0
58-59	103948.0
60-61	186826.0
62-63	185479.0
64-65	203763.0
66-67	208448.0
68-69	203225.0
70-71	194497.0
72-73	190402.0
74-75	198239.0
76-77	204664.0
78-79	205149.0
80-81	201918.0
82-83	193352.0
84-85	197741.0
86-87	204999.0
88-89	208376.0
90-91	212283.0
92-93	218184.0
94-95	246062.0
96-97	592876.0
98-99	3349010.0
100-101	6107410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.00470204806135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13149104011715	74.87464347924492
2	7.790469099447394	13.088720710274144
3	1.7996526273025721	4.535378482396902
4	0.5969953858862526	2.0060167806176823
5	0.265319711145633	1.1144051641133303
6	0.13137519247260035	0.6621680340100503
7	0.08293072864216228	0.48765998051494297
8	0.04562213591900001	0.3065979147737403
9	0.03337940559575771	0.25236243194517166
>10	0.11559704669594502	1.5713434861078166
>50	0.0037375084120251376	0.21367003105857435
>100	0.0028833589441863367	0.5409834322170459
>500	4.6637243282717053E-4	0.2702286822726923
>1k	8.038698660029713E-5	0.07582139045303361
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.372307278009684E-6
2	0.0	3.186153639004842E-5	0.0	0.0	6.372307278009684E-6
3	0.0	3.82338436680581E-5	0.0	0.0	6.372307278009684E-6
4	0.0	8.28399946141259E-5	0.0	6.372307278009684E-6	6.372307278009684E-6
5	6.372307278009684E-6	1.0195691644815494E-4	0.0	6.372307278009684E-6	6.372307278009684E-6
6	7.64676873361162E-5	1.0195691644815494E-4	0.0	6.372307278009684E-6	1.911692183402905E-5
7	7.64676873361162E-5	1.0195691644815494E-4	0.0	1.2744614556019367E-5	2.5489229112038734E-5
8	7.64676873361162E-5	1.0195691644815494E-4	0.0	1.2744614556019367E-5	2.5489229112038734E-5
9	8.921230189213557E-5	1.7205229650626145E-4	0.0	1.2744614556019367E-5	2.5489229112038734E-5
10-11	1.0832922372616463E-4	1.7205229650626145E-4	0.0	1.593076819502421E-5	2.5489229112038734E-5
12-13	1.147015310041743E-4	1.752384501452663E-4	0.0	2.2303075473033893E-5	2.8675382751043576E-5
14-15	1.306322991991985E-4	2.453338302033728E-4	0.0	2.5489229112038734E-5	3.186153639004842E-5
16-17	1.8161075742327597E-4	2.517061374813825E-4	0.0	2.5489229112038734E-5	4.4606150946067785E-5
18-19	1.8479691106228083E-4	2.6445075203740187E-4	0.0	3.82338436680581E-5	4.4606150946067785E-5
20-21	1.9116921834029052E-4	2.676369056764067E-4	0.0	5.4164611863082314E-5	4.4606150946067785E-5
22-23	1.9116921834029052E-4	2.963122884274503E-4	0.0	7.328153369711137E-5	4.4606150946067785E-5
24-25	2.0072767925730502E-4	3.3773228573451324E-4	0.0	1.529353746722324E-4	4.4606150946067785E-5
26-27	2.0391383289630988E-4	3.63221514846552E-4	0.0	3.6959382212456166E-4	4.4606150946067785E-5
28-29	2.1665844745232926E-4	3.63221514846552E-4	0.0	0.0019499260270709634	4.4606150946067785E-5
30-31	2.294030620083486E-4	3.887107439585907E-4	0.0	0.007089191846785773	4.4606150946067785E-5
32-33	2.357753692863583E-4	3.9508305123660037E-4	0.0	0.0173071865670743	4.4606150946067785E-5
34-35	2.485199838423777E-4	4.715507385727166E-4	0.0	0.03177869639543429	5.097845822407747E-5
36-37	2.5489229112038734E-4	5.416461186308231E-4	0.0	0.06199617750775621	7.009538005810653E-5
38-39	2.61264598398397E-4	5.63949194103857E-4	0.0	0.3610772334475017	7.64676873361162E-5
40-41	2.61264598398397E-4	6.244861132449491E-4	0.0	0.6674545812205683	8.28399946141259E-5
42-43	2.6445075203740187E-4	6.467891887179829E-4	0.0	0.720555017768223	8.28399946141259E-5
44-45	2.7719536659342125E-4	7.009538005810652E-4	0.0	0.7941742837510688	8.602614825313073E-5
46-47	3.1542921026147933E-4	7.26443029693104E-4	0.0	0.8682523558579314	9.239845553114042E-5
48-49	3.3773228573451324E-4	7.26443029693104E-4	0.0	0.9337819777513441	9.558460917014526E-5
50-51	3.6003536120754714E-4	8.188414852242443E-4	0.0	0.9927576816093235	9.558460917014526E-5
52-53	3.63221514846552E-4	8.347722534192685E-4	0.0	1.8035859904314708	1.0832922372616463E-4
54-55	3.63221514846552E-4	8.411445606972783E-4	0.0	2.9353746722324097	1.21073838282184E-4
56-57	3.63221514846552E-4	8.47516867975288E-4	0.0	4.178615008325738	1.2425999192118884E-4
58-59	3.63221514846552E-4	8.66633789809317E-4	0.0	5.397290099862339	1.306322991991985E-4
60-61	3.82338436680581E-4	8.761922507263314E-4	0.0	5.710393417967345	1.4656306739422272E-4
62-63	4.2057228034863913E-4	8.793784043653363E-4	0.0	5.720955517280646	1.4656306739422272E-4
64-65	4.2057228034863913E-4	9.622183989794622E-4	0.0	5.730864455097951	1.4656306739422272E-4
66-67	4.269445876266488E-4	9.749630135354816E-4	0.0	5.741203523656521	1.4656306739422272E-4
68-69	4.269445876266488E-4	9.90893781730506E-4	0.0	5.750545326126083	1.4656306739422272E-4
70-71	4.269445876266488E-4	0.0010036383962865251	0.0	5.757513444134587	1.4656306739422272E-4
72-73	4.588061240166972E-4	0.0010163830108425446	0.0	5.762933091474535	1.529353746722324E-4
74-75	4.588061240166972E-4	0.0010354999326765736	0.0	5.76717386196805	1.529353746722324E-4
76-77	4.619922776557021E-4	0.0010546168545106027	0.0	5.77184794935647	1.5612152831123726E-4
78-79	4.6517843129470694E-4	0.0010578030081496075	0.0	5.77498949684453	1.593076819502421E-4
80-81	4.7792304585072626E-4	0.0010578030081496075	0.0	5.77601225216265	1.593076819502421E-4
82-83	4.7792304585072626E-4	0.001067361469066622	0.0	5.7765985044322266	1.656799892282518E-4
84-85	4.7792304585072626E-4	0.0010769199299836365	0.0	5.776808790572401	1.656799892282518E-4
86-87	4.811091994897311E-4	0.0011024091590956754	0.0	5.776910747488849	1.656799892282518E-4
88	4.906676604067456E-4	0.0011342706954857236	0.0	5.776917119796127	1.656799892282518E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	20890	0.0	74.01075	94
TATCTCG	26885	0.0	55.836506	94
GGTATCA	25285	0.0	53.4839	1
TATGCCG	11745	0.0	41.745213	86-87
GCCGTCT	12465	0.0	41.706	90-91
ATGCCGT	12555	0.0	40.44069	88-89
TGCCGTC	12705	0.0	39.3518	88-89
CCGTCTT	13215	0.0	38.396633	90-91
CTCGTAT	12415	0.0	38.164238	82-83
TCTCGTA	12600	0.0	37.766376	82-83
TCGTATG	13765	0.0	36.094547	84-85
CGTATGC	13990	0.0	34.874535	84-85
GTATCAA	43255	0.0	34.377636	1
CTATCTC	31205	0.0	33.36308	92-93
GTATGCC	15270	0.0	33.12502	86-87
ACGCTAT	60990	0.0	28.41576	94
GCTATCT	38430	0.0	27.837564	92-93
CGCTATC	34555	0.0	27.085783	90-91
ATCAACG	54575	0.0	26.71771	3
TCAACGC	54705	0.0	26.658726	4
>>END_MODULE