FastQCFastQC Report
Fri 27 May 2016
SRR2140408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19090424
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA326250.051.9476661
GTATCAA572800.033.2854921
TCAACGC739050.025.400974
ATCAACG739850.025.3640383
TATCAAC756250.024.9532572
CAACGCA754250.024.875655
AACGCAG781050.024.089086
GTGGTAT116600.023.4501821
TGGTATC116850.022.5654892
ACGCAGA885750.021.1975257
CGCAGAG905200.020.7635278
GAGTACT612800.018.49422512-13
GTACATG621850.018.4850791
GCAGAGT1019850.018.3796469
CAGAGTA977650.017.82803510-11
TACATGG635350.017.7914832
ACATGGG646800.016.9880523
AGAGTAC925550.016.85707310-11
GTACTTT680750.016.64647114-15
AGTACTT654350.015.98025112-13
Produced by FastQC (version 0.11.5)