FastQCFastQC Report
Fri 27 May 2016
SRR2140407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18455781
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA307800.053.8369871
GTATCAA552800.034.394121
TCAACGC707900.026.4651074
ATCAACG711700.026.2860953
TATCAAC722050.026.0410772
CAACGCA721250.025.966095
AACGCAG742950.025.3015986
ACGCAGA839300.022.3165577
CGCAGAG857550.021.8323298
GTGGTAT110250.021.8264521
TGGTATC111300.020.5163632
GTACATG611600.019.5560381
GAGTACT576700.019.32092912-13
GCAGAGT966100.019.2849799
TACATGG627100.018.8640562
CAGAGTA934750.018.48285910-11
ACATGGG636100.018.1048033
GTACTTT632100.017.5661314-15
AGAGTAC881850.017.45617110-11
ACCGAAC36800.016.5747438
Produced by FastQC (version 0.11.5)