##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140407_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18455781
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35205315884492	33.0	31.0	34.0	30.0	34.0
2	32.41296794754987	34.0	31.0	34.0	30.0	34.0
3	32.442069073099645	34.0	31.0	34.0	30.0	34.0
4	36.05008647425974	37.0	35.0	37.0	35.0	37.0
5	35.96417848694672	37.0	35.0	37.0	35.0	37.0
6	36.06170678986709	37.0	35.0	37.0	35.0	37.0
7	35.99974279061937	37.0	35.0	37.0	35.0	37.0
8	36.00115123819469	37.0	35.0	37.0	35.0	37.0
9	37.72970344630769	39.0	38.0	39.0	35.0	39.0
10-11	37.67511328835123	39.0	37.0	39.0	35.0	39.0
12-13	37.65985357108431	39.0	37.0	39.0	35.0	39.0
14-15	39.027914857680635	40.0	38.0	41.0	35.0	41.0
16-17	39.03144348104261	40.0	38.0	41.0	35.0	41.0
18-19	39.08108638155167	40.5	38.5	41.0	35.0	41.0
20-21	39.09363315490792	41.0	39.0	41.0	35.0	41.0
22-23	39.046039351215	40.0	39.0	41.0	35.0	41.0
24-25	39.007266363790166	40.5	38.0	41.0	35.0	41.0
26-27	38.90652588613726	40.0	38.0	41.0	35.0	41.0
28-29	38.83795922841666	40.0	38.0	41.0	35.0	41.0
30-31	38.74354375592759	40.0	38.0	41.0	35.0	41.0
32-33	38.62240826257205	40.0	38.0	41.0	34.0	41.0
34-35	38.55347247217594	40.0	38.0	41.0	34.0	41.0
36-37	38.50683429841344	40.0	38.0	41.0	34.0	41.0
38-39	38.459452969457956	40.0	38.0	41.0	34.0	41.0
40-41	38.41394557658094	40.0	38.0	41.0	34.0	41.0
42-43	38.35064903552137	40.0	38.0	41.0	33.5	41.0
44-45	38.27569052586851	40.0	38.0	41.0	33.0	41.0
46-47	38.19696314093021	40.0	37.5	41.0	33.0	41.0
48-49	38.10546348434685	40.0	37.0	41.0	33.0	41.0
50-51	38.004862261104726	40.0	37.0	41.0	33.0	41.0
52-53	37.89352238925656	40.0	37.0	41.0	33.0	41.0
54-55	37.73190179331146	40.0	36.0	41.0	33.0	41.0
56-57	37.57271353045087	39.0	36.0	41.0	32.5	41.0
58-59	37.372502483879856	39.0	35.5	41.0	32.0	41.0
60-61	37.16360633152851	39.0	35.0	41.0	32.0	41.0
62-63	36.91276310330903	38.5	35.0	40.5	31.5	41.0
64-65	36.64930050082465	38.0	35.0	40.0	31.0	41.0
66-67	36.368247999845906	37.0	35.0	40.0	31.0	41.0
68-69	36.054152118260134	37.0	35.0	39.5	31.0	41.0
70-71	35.7408005031216	36.0	34.5	39.0	31.0	41.0
72-73	35.419561301775765	36.0	34.0	39.0	31.0	40.5
74-75	35.0975327233278	35.0	34.0	37.5	30.0	40.0
76-77	34.80269018139145	35.0	34.0	37.0	30.0	39.0
78-79	34.51460563230303	35.0	34.0	37.0	30.0	39.0
80-81	34.26878048559226	35.0	34.0	36.0	30.0	38.5
82-83	34.046968628342654	35.0	34.0	36.0	30.0	37.0
84-85	33.85024001045478	35.0	34.0	35.0	30.0	37.0
86-87	33.698140273650495	35.0	34.0	35.0	30.0	36.5
88-89	33.57631477680295	35.0	33.0	35.0	30.0	36.0
90-91	33.486539500601765	35.0	33.0	35.0	30.0	36.0
92-93	33.42649747156014	35.0	33.0	35.0	30.0	36.0
94-95	33.400105771382336	35.0	33.0	35.0	30.0	35.0
96-97	33.41930114883252	35.0	33.0	35.0	30.0	35.0
98-99	33.466380633967326	35.0	33.0	35.0	30.0	35.0
100	33.43311195908887	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	22.0
20	103.0
21	496.0
22	2326.0
23	7721.0
24	20143.0
25	42847.0
26	77737.0
27	123384.0
28	177151.0
29	239255.0
30	310095.0
31	393011.0
32	497140.0
33	640432.0
34	871526.0
35	1242643.0
36	1970256.0
37	3589402.0
38	4709332.0
39	3280905.0
40	259852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7305406365626	19.51706080604229	13.31039309580017	24.442005461594935
2	16.23650063901387	21.6222981839674	38.246959042264315	23.894242134754418
3	17.896074931645355	26.08249590088875	30.797134902753804	25.2242942647121
4	13.225298524364726	18.165940426251286	37.224430503614585	31.3843305457694
5	13.573806324954566	37.40070066082392	34.96909819319249	14.056394821029025
6	30.5238840781211	37.13140191610128	18.148258221509284	14.196455784268332
7	26.422612887772974	32.611565149294194	23.137721752813935	17.8281002101189
8	25.477729715149955	34.17096789347468	21.24065082913587	19.110651562239493
9	26.377859598572396	15.053310396346815	20.724563214095358	37.844266790985436
10-11	24.465381547889436	25.69157989299272	28.920182399818355	20.922856159299492
12-13	25.69032976713367	24.084350589118934	28.34378019548455	21.881539448262853
14-15	23.101826468356986	25.121491742885333	26.24507193708031	25.531609851677366
16-17	22.198583739154685	27.760998030915086	27.136188384550074	22.904229845380154
18-19	22.30180342950537	26.735698695167653	29.079601128773692	21.88289674655329
20-21	23.232177035447073	25.910653369731822	28.884413841371465	21.97275575344964
22-23	23.10242178504961	25.732496939074185	28.666235228853136	22.498846047023072
24-25	22.774074913529127	26.012326543533522	28.737815714278703	22.475782828658648
26-27	22.549784255208536	26.13244859814706	28.848054521397202	22.469712625247197
28-29	22.50389259070204	26.000186778093372	29.011968597926263	22.483952033278324
30-31	22.853691835003055	25.90081462396323	28.74722952512847	22.498264015905246
32-33	22.64633932355643	26.121947920494637	28.557112493553284	22.67460026239565
34-35	22.849920034722775	26.154661525194285	28.56135473216869	22.434063707914248
36-37	22.873431454786573	26.020214491259324	28.467605360693348	22.63874869326075
38-39	22.64410202606131	26.15300091545036	28.56426887090376	22.63862818758457
40-41	22.849206681020966	26.013572698411547	28.52557620942858	22.61164441113891
42-43	22.945333173263897	26.12953115710791	27.99034982599638	22.934785843631815
44-45	22.9506254907996	26.254421924936665	27.727976752248964	23.066975832014773
46-47	23.157813462345246	26.32782295376577	27.36702341316243	23.147340170726554
48-49	23.14128687227597	26.506366698470657	27.14204096874161	23.210305460511762
50-51	23.089940753300386	26.62854738973023	26.969211383945712	23.312300473023676
52-53	23.244048505720947	26.51577890089831	26.875599304168098	23.364573289212647
54-55	23.312593778927774	26.469864948260252	26.826779480363903	23.39076179244807
56-57	23.206309990076893	26.492001642820245	26.862857602368116	23.438830764734746
58-59	23.12149114487916	26.446991371467576	26.972958826134075	23.45855865751919
60-61	23.278076718219904	26.511166429540655	26.908619689600844	23.302137162638598
62-63	23.190762201854163	26.54917749144302	26.890391913465518	23.369668393237298
64-65	23.25931956888619	26.447901031796867	26.77315654822503	23.519622851091913
66-67	23.277185681058686	26.555522371445228	26.618721245836536	23.548570701659553
68-69	23.194088243601723	26.50450482707748	26.733496328072732	23.567910601248062
70-71	23.23004489642907	26.32738682173435	26.80589001352045	23.63667826831613
72-73	23.324375699760612	26.3781947997846	26.63425986092468	23.663169639530103
74-75	23.211996620775544	26.50980542256052	26.606677843492253	23.671520113171685
76-77	23.370751548558484	26.37166984247039	26.60786061378061	23.649717995190514
78-79	23.31615633904011	26.401956235561556	26.553619049826814	23.72826837557152
80-81	23.256538949291176	26.48272724983559	26.56650378415655	23.694230016716684
82-83	23.417749579809342	26.416941232108044	26.59145173288507	23.573857455197547
84-85	23.394104391243317	26.459394451217165	26.470316748212618	23.676184409326897
86-87	23.31232974125986	26.532286410279415	26.568225199252588	23.58715864920814
88-89	23.36393388944896	26.352365613992152	26.572517431781577	23.711183064777313
90-91	23.341164244377783	26.394995105592372	26.61825123863556	23.645589411394283
92-93	23.365139367000467	26.42112766579428	26.603572147806137	23.610160819399113
94-95	23.248485474892043	26.390411456569996	26.648605660749467	23.712497407788494
96-97	23.397679060814987	26.52143248833596	26.58658909802022	23.49429935282883
98-99	24.491122014191415	28.455299668559437	26.774150539396246	20.2794277778529
100	31.96783495472243	39.964562133092066	28.067602912185507	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14609.0
1	13041.0
2	12208.0
3	12743.5
4	15168.5
5	24100.0
6	31908.0
7	36995.5
8	39984.5
9	37887.0
10	31380.5
11	24241.5
12	24566.0
13	34083.0
14	59524.0
15	96035.0
16	125293.0
17	140795.0
18	144099.0
19	135782.5
20	118849.5
21	100124.0
22	86843.5
23	85000.0
24	94885.0
25	113768.0
26	139669.5
27	169019.5
28	199650.5
29	232000.5
30	265262.0
31	299997.5
32	340940.0
33	386934.0
34	427683.0
35	467744.5
36	513685.5
37	554428.5
38	591543.5
39	620847.5
40	644487.0
41	667580.5
42	687960.5
43	716806.0
44	748643.5
45	776164.0
46	798050.0
47	818493.0
48	834867.0
49	839653.0
50	840127.5
51	837168.5
52	823700.5
53	800454.5
54	770262.0
55	733308.5
56	690948.5
57	641069.0
58	586238.5
59	521589.0
60	449357.5
61	376608.5
62	309288.0
63	250144.0
64	197483.0
65	153756.5
66	116737.5
67	89849.0
68	69728.5
69	53415.0
70	40988.5
71	31129.0
72	23447.0
73	17491.5
74	12662.0
75	9033.0
76	6507.5
77	4475.0
78	3120.0
79	2232.0
80	1570.0
81	1089.0
82	788.0
83	571.5
84	407.5
85	279.5
86	190.0
87	132.5
88	80.5
89	48.5
90	34.5
91	26.0
92	20.5
93	12.5
94	6.0
95	3.5
96	2.5
97	3.0
98	2.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0030396979678074853
4	5.418356448854698E-6
5	0.11168858148024188
6	4.1179509011295704E-4
7	2.167342579541879E-4
8	0.0
9	0.0
10-11	0.012806285466868076
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.211872553959966E-5
22-23	1.354961334552365E-5
24-25	4.2831000069424175E-4
26-27	0.0
28-29	2.7427356338494634E-4
30-31	0.0
32-33	5.197034058133586E-5
34-35	8.478028459805653E-4
36-37	9.578683148739152E-4
38-39	2.2737431204182496E-4
40-41	0.0023573610530212537
42-43	0.001334213645963361
44-45	0.001995071618105319
46-47	5.266371743898306E-4
48-49	0.0053104695680591145
50-51	0.0019403329801743101
52-53	4.322652618481032E-4
54-55	5.167898075941128E-4
56-57	8.952690879922577E-5
58-59	0.00391991522339639
60-61	0.002903365145042181
62-63	0.002970389356634358
64-65	7.753712301383072E-4
66-67	0.0010698149175473691
68-69	0.004947501761607827
70-71	0.0027608676117077415
72-73	0.0
74-75	0.0015691157545124268
76-77	0.0015061105589534171
78-79	1.4417317839370667E-4
80-81	7.72418659873708E-6
82-83	9.433203603200781E-5
84-85	3.9993748177284925E-5
86-87	0.0012176279955074007
88-89	1.0792761543896236E-4
90-91	0.0013043113885906547
92-93	0.0016079476877560966
94-95	6.095386706571131E-4
96-97	1.4528663372958146E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	783.0
20-21	3054.0
22-23	5727.0
24-25	8364.0
26-27	17515.0
28-29	37573.0
30-31	79162.0
32-33	106362.0
34-35	142695.0
36-37	191315.0
38-39	207262.0
40-41	209530.0
42-43	253597.0
44-45	311180.0
46-47	293509.0
48-49	247388.0
50-51	212894.0
52-53	205197.0
54-55	226463.0
56-57	231466.0
58-59	221317.0
60-61	210612.0
62-63	209860.0
64-65	228640.0
66-67	235807.0
68-69	230919.0
70-71	219690.0
72-73	214923.0
74-75	223325.0
76-77	232387.0
78-79	233260.0
80-81	227942.0
82-83	219091.0
84-85	222094.0
86-87	231264.0
88-89	236376.0
90-91	239862.0
92-93	242253.0
94-95	274949.0
96-97	590344.0
98-99	3552142.0
100-101	6967688.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.19124061354552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.59818914162872	76.32980889702455
2	7.40696594612666	12.620172362656282
3	1.724874372691631	4.408325631363334
4	0.6063205394374528	2.0661279585660304
5	0.26163825012317243	1.1144643559975096
6	0.13633404267555552	0.6968679740034381
7	0.07929870704935517	0.47288886628092713
8	0.0493400336220708	0.3362670940942607
9	0.030722058614680966	0.23555252588280617
>10	0.10316569230128965	1.3952517995375437
>50	0.0021549274596295302	0.12237601251726105
>100	9.30538411799876E-4	0.13950933598406232
>500	3.2921472195976166E-5	0.017677433336979957
>1k	3.282838587480466E-5	0.04470975275503494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0836712897709396E-5
2	0.0	5.418356448854698E-6	0.0	0.0	1.0836712897709396E-5
3	5.418356448854698E-6	5.418356448854698E-6	0.0	5.418356448854698E-6	1.0836712897709396E-5
4	5.418356448854698E-6	3.792849514198288E-5	0.0	5.418356448854698E-6	1.0836712897709396E-5
5	1.0836712897709396E-5	3.792849514198288E-5	0.0	5.418356448854698E-6	1.0836712897709396E-5
6	1.6255069346564094E-5	3.792849514198288E-5	0.0	1.6255069346564094E-5	1.0836712897709396E-5
7	1.6255069346564094E-5	3.792849514198288E-5	0.0	3.251013869312819E-5	1.0836712897709396E-5
8	1.6255069346564094E-5	3.792849514198288E-5	0.0	3.251013869312819E-5	1.0836712897709396E-5
9	2.167342579541879E-5	7.043863383511107E-5	0.0	3.251013869312819E-5	1.0836712897709396E-5
10-11	3.251013869312819E-5	7.043863383511107E-5	0.0	4.605602981526493E-5	1.0836712897709396E-5
12-13	3.251013869312819E-5	7.585699028396576E-5	0.0	5.4183564488546976E-5	1.0836712897709396E-5
14-15	4.605602981526493E-5	9.211205963052986E-5	0.0	5.960192093740168E-5	1.0836712897709396E-5
16-17	6.772945561068372E-5	9.211205963052986E-5	0.0	7.314781205953842E-5	1.0836712897709396E-5
18-19	8.669370318167515E-5	1.0294877252823925E-4	0.0	8.127534673282046E-5	1.3545891122136744E-5
20-21	1.0836712897709395E-4	1.1378548542594865E-4	0.0	8.398452495724781E-5	1.6255069346564094E-5
22-23	1.0836712897709395E-4	1.1378548542594865E-4	0.0	1.0836712897709395E-4	1.896424757099144E-5
24-25	1.3545891122136743E-4	1.3004055477251275E-4	0.0	1.8151494103663238E-4	2.167342579541879E-5
26-27	1.5171398056793153E-4	1.4087726767022214E-4	0.0	3.982491989908203E-4	2.167342579541879E-5
28-29	1.5171398056793153E-4	1.435864458946495E-4	0.0	0.001622797756431982	2.167342579541879E-5
30-31	1.5171398056793153E-4	1.4629562411907685E-4	0.0	0.0049875971111707496	2.167342579541879E-5
32-33	1.5171398056793153E-4	1.5171398056793153E-4	0.0	0.01121057949268037	2.7091782244273488E-5
34-35	1.6255069346564094E-4	1.8693329748548706E-4	0.0	0.020424494633957783	2.7091782244273488E-5
36-37	1.7338740636335033E-4	2.221526144030426E-4	0.0	0.035837009552724966	2.7091782244273488E-5
38-39	1.7338740636335033E-4	2.275709708518973E-4	0.0	0.06663494760801507	2.7091782244273488E-5
40-41	1.7338740636335033E-4	2.438260401984614E-4	0.0	0.09725137072226854	2.7091782244273488E-5
42-43	1.7338740636335033E-4	2.438260401984614E-4	0.0	0.11767315617800189	2.7091782244273488E-5
44-45	1.7609658458777767E-4	2.600811095450255E-4	0.0	0.14387090960821436	2.980096046870084E-5
46-47	1.78805762812205E-4	2.763361788915896E-4	0.0	0.17048587648498864	3.5219316917555535E-5
48-49	1.78805762812205E-4	2.763361788915896E-4	0.0	0.19357078413533407	4.8765208039692276E-5
50-51	1.78805762812205E-4	3.0884631758471774E-4	0.0	0.21370810587750255	4.8765208039692276E-5
52-53	1.8422411926105972E-4	3.1426467403357247E-4	0.0	0.2596882787024835	6.231109916182902E-5
54-55	1.8964247570991442E-4	3.1426467403357247E-4	0.0	0.31404523059739387	7.043863383511107E-5
56-57	1.8964247570991442E-4	3.1426467403357247E-4	0.0	0.36862704428493165	7.043863383511107E-5
58-59	1.950608321587691E-4	3.169738522579998E-4	0.0	0.4031988676068491	7.043863383511107E-5
60-61	1.950608321587691E-4	3.1968303048242715E-4	0.0	0.4189229380214254	7.585699028396576E-5
62-63	1.950608321587691E-4	3.223922087068545E-4	0.0	0.4355464556065116	8.669370318167517E-5
64-65	1.950608321587691E-4	3.4677481272670066E-4	0.0	0.44596866423588355	8.669370318167517E-5
66-67	1.950608321587691E-4	3.49483990951128E-4	0.0	0.45562959378419154	8.669370318167517E-5
68-69	1.950608321587691E-4	3.603207038488374E-4	0.0	0.46502231468828115	8.940288140610252E-5
70-71	2.004791886076238E-4	3.657390602976921E-4	0.0	0.4726811615287373	9.211205963052986E-5
72-73	2.004791886076238E-4	3.738665949709741E-4	0.0	0.478302706344424	1.0836712897709395E-4
74-75	2.004791886076238E-4	3.9554002076639294E-4	0.0	0.48283787069211537	1.0836712897709395E-4
76-77	2.004791886076238E-4	4.172134465618117E-4	0.0	0.4873621983269091	1.0836712897709395E-4
78-79	2.0589754505647852E-4	4.172134465618117E-4	0.0	0.490347712730228	1.0836712897709395E-4
80-81	2.0589754505647852E-4	4.172134465618117E-4	0.0	0.49121464976204476	1.0836712897709395E-4
82-83	2.0589754505647852E-4	4.172134465618117E-4	0.0	0.4917483578722569	1.0836712897709395E-4
84-85	2.0589754505647852E-4	4.172134465618117E-4	0.0	0.4919109085657226	1.0836712897709395E-4
86-87	2.0589754505647852E-4	4.226318030106664E-4	0.0	0.4919732196648844	1.1107630720152129E-4
88	2.0589754505647852E-4	4.334685159083758E-4	0.0	0.49198134719955766	1.1378548542594865E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30780	0.0	53.836987	1
GTATCAA	55280	0.0	34.39412	1
TCAACGC	70790	0.0	26.465107	4
ATCAACG	71170	0.0	26.286095	3
TATCAAC	72205	0.0	26.041077	2
CAACGCA	72125	0.0	25.96609	5
AACGCAG	74295	0.0	25.301598	6
ACGCAGA	83930	0.0	22.316557	7
CGCAGAG	85755	0.0	21.832329	8
GTGGTAT	11025	0.0	21.826452	1
TGGTATC	11130	0.0	20.516363	2
GTACATG	61160	0.0	19.556038	1
GAGTACT	57670	0.0	19.320929	12-13
GCAGAGT	96610	0.0	19.284979	9
TACATGG	62710	0.0	18.864056	2
CAGAGTA	93475	0.0	18.482859	10-11
ACATGGG	63610	0.0	18.104803	3
GTACTTT	63210	0.0	17.56613	14-15
AGAGTAC	88185	0.0	17.456171	10-11
ACCGAAC	3680	0.0	16.574743	8
>>END_MODULE