FastQCFastQC Report
Fri 27 May 2016
SRR2140406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20171593
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212800.048.3330961
GTATCAA393000.037.2511441
TCAACGC472600.029.872494
ATCAACG475950.029.6667633
CAACGCA481250.029.3111765
AACGCAG502800.028.1675976
TATCAAC516000.027.6696552
ACGCAGA581600.024.299867
CGCAGAG599700.023.566338
GTACATG592700.022.6368891
TACATGG605450.021.7520282
ACATGGG609450.021.0363
GCAGAGT726350.019.4337489
AGAGTAC630000.018.63381810-11
CATGGGG419450.017.7889214
GAGTACT379400.017.58272612-13
CAGAGTA688850.017.45897510-11
GTACTTT422500.015.77463414-15
AGTACTT429150.015.48987412-13
ACCGAAC27050.014.0058898
Produced by FastQC (version 0.11.5)