##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20171593
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37066963427232	33.0	31.0	34.0	31.0	34.0
2	32.43865028409011	34.0	31.0	34.0	31.0	34.0
3	32.47867478785637	34.0	31.0	34.0	31.0	34.0
4	36.06222691485001	37.0	35.0	37.0	35.0	37.0
5	35.97366682938725	37.0	35.0	37.0	35.0	37.0
6	36.07149846816758	37.0	35.0	37.0	35.0	37.0
7	36.010672285525494	37.0	35.0	37.0	35.0	37.0
8	36.01106689987251	37.0	35.0	37.0	35.0	37.0
9	37.73716518075692	39.0	38.0	39.0	35.0	39.0
10-11	37.67720172125226	39.0	37.0	39.0	35.0	39.0
12-13	37.66057817049948	39.0	37.0	39.0	35.0	39.0
14-15	39.05667162727306	40.0	38.0	41.0	36.0	41.0
16-17	39.063241386042236	40.0	38.0	41.0	36.0	41.0
18-19	39.08800787325027	40.0	38.5	41.0	36.0	41.0
20-21	39.09355273642929	40.0	39.0	41.0	35.0	41.0
22-23	39.04947101926838	40.0	39.0	41.0	35.0	41.0
24-25	39.01920348062678	40.0	38.0	41.0	35.0	41.0
26-27	38.929057976331094	40.0	38.0	41.0	35.0	41.0
28-29	38.86566675171176	40.0	38.0	41.0	35.0	41.0
30-31	38.792264211743685	40.0	38.0	41.0	35.0	41.0
32-33	38.70244047158871	40.0	38.0	41.0	34.5	41.0
34-35	38.64150062987567	40.0	38.0	41.0	34.0	41.0
36-37	38.59770155683657	40.0	38.0	41.0	34.0	41.0
38-39	38.55046250254702	40.0	38.0	41.0	34.0	41.0
40-41	38.50601377158547	40.0	38.0	41.0	34.0	41.0
42-43	38.44759294607479	40.0	38.0	41.0	34.0	41.0
44-45	38.37900275234509	40.0	38.0	41.0	33.5	41.0
46-47	38.305323830479814	40.0	38.0	41.0	33.0	41.0
48-49	38.21597677909808	40.0	37.5	41.0	33.0	41.0
50-51	38.110506241593825	40.0	37.0	41.0	33.0	41.0
52-53	37.995389834583726	40.0	37.0	41.0	33.0	41.0
54-55	37.83690543984209	40.0	36.5	41.0	33.0	41.0
56-57	37.682581173561445	39.5	36.0	41.0	33.0	41.0
58-59	37.48532695048904	39.0	36.0	41.0	32.0	41.0
60-61	37.27458868362779	39.0	35.0	41.0	32.0	41.0
62-63	37.02363662788376	38.5	35.0	40.5	32.0	41.0
64-65	36.76019867117999	38.0	35.0	40.0	31.0	41.0
66-67	36.477335977305955	37.0	35.0	40.0	31.0	41.0
68-69	36.1625056359783	37.0	35.0	39.5	31.0	41.0
70-71	35.840898235233894	36.0	34.5	39.0	31.0	41.0
72-73	35.5103874656763	36.0	34.0	39.0	31.0	40.5
74-75	35.17908763982905	35.0	34.0	38.0	30.0	40.0
76-77	34.87384388853275	35.0	34.0	37.0	30.0	39.0
78-79	34.57115701343249	35.0	34.0	37.0	30.0	39.0
80-81	34.307705967860215	35.0	34.0	36.0	30.0	39.0
82-83	34.07307517323289	35.0	34.0	36.0	30.0	37.0
84-85	33.8621396268782	35.0	34.0	35.5	30.0	37.0
86-87	33.69425933216319	35.0	33.5	35.0	30.0	36.5
88-89	33.557558920159686	35.0	33.0	35.0	29.5	36.0
90-91	33.45347654828768	35.0	33.0	35.0	29.0	36.0
92-93	33.384282759942124	35.0	33.0	35.0	29.0	36.0
94-95	33.35174486048681	35.0	33.0	35.0	29.0	35.0
96-97	33.36500676008599	35.0	33.0	35.0	30.0	35.0
98-99	33.413671820974415	35.0	33.0	35.0	30.0	35.0
100	33.42407459701024	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	17.0
20	96.0
21	567.0
22	2422.0
23	8426.0
24	22682.0
25	48305.0
26	88137.0
27	140120.0
28	200994.0
29	270328.0
30	347508.0
31	438938.0
32	553457.0
33	711835.0
34	970586.0
35	1384116.0
36	2222532.0
37	4097472.0
38	5474307.0
39	2996769.0
40	191975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.510440300872624	19.513277905220473	12.916922327354117	25.059359466552788
2	15.385909283416535	21.36729607820265	39.33885638085202	23.907938257528794
3	17.86176601396472	26.46303180317578	30.135323665348217	25.539878517511276
4	12.620650498019714	17.908894742115287	38.44253534587331	31.02791941399169
5	13.306574427435928	37.931560562021396	35.37797182051466	13.383893190028019
6	30.315716802203426	37.980104332210445	18.563750776031977	13.14042808955415
7	26.81644268940867	32.19297265424726	23.440528245188197	17.550056411155875
8	25.381128798305618	35.359934141046764	21.098006488629828	18.16093057201779
9	26.36257830504512	15.515571824198515	20.428198209234143	37.69365166152222
10-11	24.484167143864454	25.942831808312576	29.04714400129843	20.525857046524536
12-13	25.568446676472206	23.726279327567237	28.719652929741347	21.985621066219213
14-15	22.818011943826153	24.814068973134646	26.033680136219285	26.334238946819916
16-17	22.579319838547207	27.757906874286032	26.67157472391992	22.99119856324684
18-19	22.68276233810587	27.16480051922523	28.048488783211123	22.103948359457778
20-21	23.444414530354894	26.379328795089695	27.81438625001367	22.361870424541745
22-23	23.180760356987662	26.24914494596376	27.68874704263856	22.881347654410018
24-25	22.932797091251874	26.484990088411802	27.739260264043203	22.84295255629312
26-27	22.905435957269543	26.589259940803995	27.67452912437982	22.830774977546643
28-29	22.86950424268383	26.463485861439256	27.82361764596898	22.84339224990793
30-31	23.108827260056614	26.42781413605897	27.628926818007322	22.834431785877094
32-33	22.914197921280063	26.589130647786668	27.587997754237666	22.908673676695603
34-35	23.09284364585993	26.571254538853438	27.595809333552744	22.740092481733885
36-37	23.134594167332885	26.49973037997246	27.49610611522289	22.869569337471766
38-39	22.97980120745981	26.633201718756737	27.546156677640386	22.84084039614306
40-41	23.107141298024647	26.470521498209624	27.561284948359834	22.861052255405898
42-43	23.107507740209048	26.562383290598284	27.29106009720137	23.039048871991294
44-45	23.07523818628067	26.677321960267996	27.143615990472576	23.103823862978757
46-47	23.24323541379171	26.676336685906588	26.895952168848847	23.184475731452856
48-49	23.21620818553334	26.802283380333826	26.780511306755344	23.200997127377494
50-51	23.176739720450357	26.87475961023041	26.66322455428792	23.28527611503132
52-53	23.298279910640773	26.75564063315578	26.668543347047986	23.27753610915546
54-55	23.311128141937445	26.71821221774085	26.72707830773709	23.24358133258462
56-57	23.263293121602146	26.750210949445012	26.70291349960176	23.28358242935108
58-59	23.245632150562408	26.72313890512516	26.732711644674563	23.298517299637872
60-61	23.34098217477634	26.820028456205097	26.649002229352515	23.18998713966605
62-63	23.247152700122093	26.87889080822556	26.700865548454484	23.173090943197863
64-65	23.308808889146427	26.69111678557356	26.631939839042857	23.36813448623716
66-67	23.339532604368017	26.77449665709282	26.57498399635224	23.31098674218693
68-69	23.26300787351439	26.792054333259617	26.61974527394265	23.325192519283338
70-71	23.309598784199437	26.575653060944436	26.71119558911356	23.403552565742565
72-73	23.32930421821748	26.717058840275016	26.527770185467954	23.425866756039554
74-75	23.193488710459317	26.80498957034817	26.577263411883617	23.424258307308897
76-77	23.36315911851213	26.718874243836943	26.588146266608813	23.32982037104211
78-79	23.30911164706409	26.6992783798349	26.54868608046629	23.442923892634724
80-81	23.24713417502094	26.819649865162482	26.51827665190677	23.414939307909815
82-83	23.36278089040845	26.73723900191848	26.559495875882895	23.340484231790178
84-85	23.304735450498328	26.791206402152557	26.50328657599425	23.400771571354866
86-87	23.233461608169986	26.817436370343394	26.61992229301476	23.329179728471864
88-89	23.330591233593672	26.67098795307971	26.599870860430595	23.39854995289602
90-91	23.342805832818748	26.733764310795323	26.600724665556623	23.32270519082931
92-93	23.347228253350448	26.80976004728527	26.495446397815204	23.347565301549082
94-95	23.199915105582992	26.69983674201167	26.647770840846054	23.45247731155929
96-97	23.340306576419486	26.871452459463143	26.61471109021453	23.173529873902844
98-99	24.436724846923052	28.790300285692112	26.80006937138002	19.97290549600482
100	23.360099515022213	29.644155129407025	20.51912320481431	26.476622150756455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3423.0
1	3437.0
2	4807.0
3	6254.0
4	8558.0
5	15551.5
6	21125.0
7	23800.5
8	24324.5
9	21697.5
10	17526.5
11	13278.5
12	13609.0
13	19563.0
14	35701.5
15	58617.0
16	76551.5
17	85562.5
18	86161.0
19	79770.0
20	69120.0
21	59204.0
22	54446.5
23	58071.5
24	69747.5
25	89039.5
26	115226.0
27	145044.5
28	178247.0
29	215469.0
30	255273.5
31	295729.0
32	343107.0
33	393139.0
34	438397.0
35	482093.0
36	530219.5
37	576090.0
38	614901.0
39	648062.0
40	673080.0
41	695736.0
42	716259.5
43	744259.5
44	777431.0
45	808878.5
46	831036.5
47	847172.5
48	865001.5
49	871919.5
50	872268.0
51	867002.0
52	851199.5
53	826107.0
54	792886.0
55	751494.0
56	705377.0
57	651887.0
58	590422.0
59	523259.5
60	448800.0
61	372910.5
62	303629.5
63	242718.5
64	188181.5
65	142351.0
66	105752.5
67	79426.0
68	60649.0
69	45962.5
70	35060.0
71	26789.0
72	20584.0
73	15943.0
74	11718.5
75	8381.5
76	5890.5
77	3860.0
78	2620.5
79	1824.5
80	1239.5
81	846.0
82	618.0
83	462.5
84	345.5
85	246.5
86	179.5
87	137.5
88	93.5
89	61.0
90	42.0
91	32.0
92	29.0
93	20.5
94	11.5
95	8.5
96	6.0
97	3.5
98	2.0
99	3.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0030736293360668146
4	9.914933342151015E-6
5	0.11173138383269979
6	3.2719280029098346E-4
7	2.5283080022485085E-4
8	0.0
9	0.0
10-11	0.01305053101160627
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.932281517761754E-5
22-23	2.4791354719116185E-5
24-25	4.2401540956305515E-4
26-27	0.0
28-29	3.47463766416391E-4
30-31	0.0
32-33	3.486897980837005E-5
34-35	8.216690460936854E-4
36-37	8.724030778982245E-4
38-39	2.3193274777205276E-4
40-41	0.0022080977288826543
42-43	0.0012572355246003046
44-45	0.0017825480457876832
46-47	5.148856965455617E-4
48-49	0.005138323241785453
50-51	0.0019429336561744366
52-53	3.8152441093231254E-4
54-55	4.4625966387399523E-4
56-57	9.757071417182372E-5
58-59	0.0036955641285475738
60-61	0.0027602793866002913
62-63	0.002603470281247665
64-65	6.703253905290483E-4
66-67	9.307731658266038E-4
68-69	0.004194196853273118
70-71	0.0023495100608471216
72-73	0.0
74-75	0.001289625695689169
76-77	0.0011652414696096574
78-79	1.2576041634602752E-4
80-81	6.0521566996572755E-6
82-83	1.162398192654347E-4
84-85	5.254290669214276E-5
86-87	8.838708069465623E-4
88-89	6.948416534830991E-5
90-91	9.497031458197234E-4
92-93	0.0011893770930768272
94-95	4.1763038165662134E-4
96-97	1.140788117497284E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	519.0
20-21	1881.0
22-23	3761.0
24-25	5211.0
26-27	9679.0
28-29	21208.0
30-31	42334.0
32-33	51340.0
34-35	67799.0
36-37	101735.0
38-39	133416.0
40-41	131448.0
42-43	147925.0
44-45	179785.0
46-47	178293.0
48-49	167190.0
50-51	152690.0
52-53	140240.0
54-55	148328.0
56-57	155032.0
58-59	158947.0
60-61	159317.0
62-63	149086.0
64-65	151354.0
66-67	159940.0
68-69	165985.0
70-71	169652.0
72-73	158484.0
74-75	155787.0
76-77	162141.0
78-79	173359.0
80-81	178824.0
82-83	170213.0
84-85	166766.0
86-87	174857.0
88-89	189445.0
90-91	205816.0
92-93	217517.0
94-95	257215.0
96-97	691082.0
98-99	1273539.0
100-101	1.3042453E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.68062175807506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.5813225075928	71.53696876814675
2	8.309530862170227	13.574552946799557
3	2.2119916808638083	5.420305674499363
4	0.8425741886545235	2.752879344264285
5	0.4089984514098478	1.6703623904623113
6	0.2146594687046414	1.05201113220319
7	0.12491699962672628	0.7142308738364964
8	0.07914108632357841	0.5171434510015501
9	0.05438122384037466	0.3997702957722171
>10	0.16875765435315646	2.0495500439574887
>50	0.0029063719903424075	0.15598515833424503
>100	7.495844249937631E-4	0.09182036893088343
>500	4.665478221320508E-5	0.027382293183578817
>1k	2.3265262729180356E-5	0.03703725860798931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.957466671075507E-6
2	0.0	2.4787333355377534E-5	0.0	0.0	4.957466671075507E-6
3	0.0	2.4787333355377534E-5	0.0	0.0	4.957466671075507E-6
4	0.0	3.470226669752855E-5	0.0	4.957466671075507E-6	4.957466671075507E-6
5	0.0	3.965973336860406E-5	0.0	4.957466671075507E-6	4.957466671075507E-6
6	0.0	3.965973336860406E-5	0.0	9.914933342151015E-6	4.957466671075507E-6
7	0.0	3.965973336860406E-5	0.0	9.914933342151015E-6	9.914933342151015E-6
8	4.957466671075507E-6	4.461720003967956E-5	0.0	9.914933342151015E-6	9.914933342151015E-6
9	4.957466671075507E-6	7.436200006613261E-5	0.0	1.982986668430203E-5	1.4872400013226521E-5
10-11	4.957466671075507E-6	7.436200006613261E-5	0.0	2.9744800026453042E-5	1.4872400013226521E-5
12-13	4.957466671075507E-6	8.179820007274587E-5	0.0	3.965973336860405E-5	1.4872400013226521E-5
14-15	7.436200006613261E-6	1.1897920010581217E-4	0.0	4.461720003967956E-5	1.7351133348764275E-5
16-17	2.9744800026453042E-5	1.3385160011903868E-4	0.0	4.957466671075507E-5	2.4787333355377534E-5
18-19	3.2223533361990797E-5	1.586389334744162E-4	0.0	5.205340004629282E-5	2.4787333355377534E-5
20-21	3.7181000033066305E-5	1.9086246683640702E-4	0.0	5.453213338183058E-5	2.726606669091529E-5
22-23	3.965973336860406E-5	2.0077740017855803E-4	0.0	7.436200006613261E-5	2.9744800026453042E-5
24-25	3.965973336860406E-5	2.0821360018517132E-4	0.0	9.667060008597238E-5	3.470226669752855E-5
26-27	3.965973336860406E-5	2.131710668562468E-4	0.0	1.4128780012565196E-4	3.470226669752855E-5
28-29	3.965973336860406E-5	2.1564980019178457E-4	0.0	5.031828671141639E-4	3.7181000033066305E-5
30-31	3.965973336860406E-5	2.553095335603886E-4	0.0	0.0016483576681326061	3.965973336860406E-5
32-33	3.965973336860406E-5	2.5778826689592637E-4	0.0	0.0035594610698322137	3.965973336860406E-5
34-35	4.957466671075507E-5	2.9744800026453044E-4	0.0	0.0060828116054096465	3.965973336860406E-5
36-37	4.957466671075507E-5	3.3958646696867224E-4	0.0	0.009862879942104722	3.965973336860406E-5
38-39	5.205340004629282E-5	3.569376003174365E-4	0.0	0.015712690613973818	3.965973336860406E-5
40-41	5.453213338183058E-5	4.189059337058803E-4	0.0	0.02250937742001834	4.213846670414181E-5
42-43	5.701086671736833E-5	4.511294670678711E-4	0.0	0.028815275025626384	4.957466671075507E-5
44-45	5.9489600052906085E-5	4.957466671075507E-4	0.0	0.03587718629857344	5.453213338183058E-5
46-47	5.9489600052906085E-5	5.155765337918527E-4	0.0	0.04393554837240668	5.453213338183058E-5
48-49	5.9489600052906085E-5	5.23012733798466E-4	0.0	0.05165680271260678	7.931946673720812E-5
50-51	5.9489600052906085E-5	5.701086671736833E-4	0.0	0.06009688972011283	8.179820007274587E-5
52-53	5.9489600052906085E-5	5.701086671736833E-4	0.0	0.06823209252734774	9.419186675043464E-5
54-55	5.9489600052906085E-5	5.849810671869098E-4	0.0	0.07543529160042045	1.0410680009258565E-4
56-57	5.9489600052906085E-5	6.097684005422874E-4	0.0	0.08299047080713953	1.0410680009258565E-4
58-59	5.9489600052906085E-5	6.097684005422874E-4	0.0	0.08995323274666507	1.0906426676366115E-4
60-61	5.9489600052906085E-5	6.221620672199761E-4	0.0	0.09701762275294767	1.1402173343473666E-4
62-63	5.9489600052906085E-5	6.395132005687404E-4	0.0	0.10358130862545165	1.2393666677688766E-4
64-65	5.9489600052906085E-5	6.94045333950571E-4	0.0	0.10922538443047111	1.2393666677688766E-4
66-67	6.196833338844383E-5	7.014815339571842E-4	0.0	0.11538751550261797	1.2641540011242543E-4
68-69	6.444706672398159E-5	7.188326673059485E-4	0.0	0.12103407004097297	1.3137286678350092E-4
70-71	6.444706672398159E-5	7.287476006480995E-4	0.0	0.12570152491179054	1.388090667901142E-4
72-73	6.444706672398159E-5	7.33705067319175E-4	0.0	0.12946424211513685	1.5863893347441624E-4
74-75	6.444706672398159E-5	7.609711340100904E-4	0.0	0.1324858180511574	1.61117666809954E-4
76-77	6.444706672398159E-5	7.907159340365434E-4	0.0	0.13528182925364396	1.7103260015210499E-4
78-79	6.444706672398159E-5	8.031096007142321E-4	0.0	0.1370962620552576	1.7351133348764275E-4
80-81	6.444706672398159E-5	8.031096007142321E-4	0.0	0.13778287118920157	1.80947533494256E-4
82-83	6.444706672398159E-5	8.055883340497699E-4	0.0	0.13824887305628267	2.1317106685624679E-4
84-85	6.444706672398159E-5	8.105458007208454E-4	0.0	0.138370330989724	2.181285335273223E-4
86-87	6.444706672398159E-5	8.155032673919209E-4	0.0	0.13841246945642816	2.181285335273223E-4
88	6.444706672398159E-5	8.576417340960627E-4	0.0	0.13841742692309922	2.2308600019839783E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21280	0.0	48.333096	1
GTATCAA	39300	0.0	37.251144	1
TCAACGC	47260	0.0	29.87249	4
ATCAACG	47595	0.0	29.666763	3
CAACGCA	48125	0.0	29.311176	5
AACGCAG	50280	0.0	28.167597	6
TATCAAC	51600	0.0	27.669655	2
ACGCAGA	58160	0.0	24.29986	7
CGCAGAG	59970	0.0	23.56633	8
GTACATG	59270	0.0	22.636889	1
TACATGG	60545	0.0	21.752028	2
ACATGGG	60945	0.0	21.036	3
GCAGAGT	72635	0.0	19.433748	9
AGAGTAC	63000	0.0	18.633818	10-11
CATGGGG	41945	0.0	17.788921	4
GAGTACT	37940	0.0	17.582726	12-13
CAGAGTA	68885	0.0	17.458975	10-11
GTACTTT	42250	0.0	15.774634	14-15
AGTACTT	42915	0.0	15.489874	12-13
ACCGAAC	2705	0.0	14.005889	8
>>END_MODULE