FastQCFastQC Report
Fri 27 May 2016
SRR2140405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43405925
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA490800.050.2778471
GTATCAA896150.037.8405041
TCAACGC1101400.030.0557544
ATCAACG1107500.029.9050663
CAACGCA1125750.029.4082955
AACGCAG1169850.028.3386366
TATCAAC1188950.028.122482
ACGCAGA1345900.024.5745727
CGCAGAG1392600.023.7504228
GTACATG1400000.022.8814031
TACATGG1433350.021.90462
ACATGGG1444850.021.1236783
GCAGAGT1656500.019.9615449
AGAGTAC1457750.018.67431310-11
GAGTACT838600.018.17212-13
CAGAGTA1577450.018.03707510-11
CATGGGG947450.017.442664
GTACTTT934850.016.23582814-15
AGTACTT941900.015.90927812-13
GTGGTAT244800.014.4951121
Produced by FastQC (version 0.11.5)