##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140405_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43405925
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30745832510193	33.0	31.0	34.0	30.0	34.0
2	32.37077062636034	34.0	31.0	34.0	30.0	34.0
3	32.410902106106484	34.0	31.0	34.0	30.0	34.0
4	36.01283785566141	37.0	35.0	37.0	35.0	37.0
5	35.9207119304565	37.0	35.0	37.0	35.0	37.0
6	36.0266924619162	37.0	35.0	37.0	35.0	37.0
7	35.96609718604085	37.0	35.0	37.0	35.0	37.0
8	35.965094604020074	37.0	35.0	37.0	35.0	37.0
9	37.67758355570121	39.0	37.0	39.0	35.0	39.0
10-11	37.61382846927003	39.0	37.0	39.0	35.0	39.0
12-13	37.59861748136919	39.0	37.0	39.0	35.0	39.0
14-15	38.97364545278093	40.0	38.0	41.0	35.0	41.0
16-17	38.9798750055436	40.0	38.0	41.0	35.0	41.0
18-19	39.005954302321626	40.0	38.5	41.0	35.0	41.0
20-21	39.01251276941428	40.0	38.5	41.0	35.0	41.0
22-23	38.96639323379651	40.0	38.0	41.0	35.0	41.0
24-25	38.9334541808669	40.0	38.0	41.0	35.0	41.0
26-27	38.839274339369126	40.0	38.0	41.0	35.0	41.0
28-29	38.770582401428214	40.0	38.0	41.0	34.5	41.0
30-31	38.69426856254822	40.0	38.0	41.0	34.0	41.0
32-33	38.59878904925004	40.0	38.0	41.0	34.0	41.0
34-35	38.53340394111575	40.0	38.0	41.0	34.0	41.0
36-37	38.4856486316117	40.0	38.0	41.0	34.0	41.0
38-39	38.43467017566471	40.0	38.0	41.0	34.0	41.0
40-41	38.38609760007437	40.0	38.0	41.0	34.0	41.0
42-43	38.32332998286291	40.0	38.0	41.0	33.0	41.0
44-45	38.250357825813026	40.0	38.0	41.0	33.0	41.0
46-47	38.17068517784371	40.0	37.5	41.0	33.0	41.0
48-49	38.07624623583658	40.0	37.0	41.0	33.0	41.0
50-51	37.96598623552694	40.0	37.0	41.0	33.0	41.0
52-53	37.8460792708629	40.0	37.0	41.0	33.0	41.0
54-55	37.67888188450411	40.0	36.0	41.0	32.5	41.0
56-57	37.520102395916965	39.0	36.0	41.0	32.0	41.0
58-59	37.318744431788474	39.0	35.5	41.0	32.0	41.0
60-61	37.10785956938864	39.0	35.0	41.0	31.5	41.0
62-63	36.85399103143106	38.0	35.0	40.0	31.0	41.0
64-65	36.59232658987622	38.0	35.0	40.0	31.0	41.0
66-67	36.31037467803968	37.0	35.0	40.0	31.0	41.0
68-69	35.99678967299417	37.0	34.5	39.5	31.0	41.0
70-71	35.677859661956305	36.0	34.0	39.0	30.5	41.0
72-73	35.35264438237326	36.0	34.0	39.0	30.0	40.5
74-75	35.02603612553408	35.0	34.0	37.5	30.0	40.0
76-77	34.72919955619504	35.0	34.0	37.0	30.0	39.0
78-79	34.43325674580866	35.0	34.0	37.0	30.0	39.0
80-81	34.1747788748369	35.0	34.0	36.0	30.0	38.5
82-83	33.94414427777029	35.0	33.5	36.0	29.5	37.0
84-85	33.73673383839153	35.0	33.0	35.5	29.0	37.0
86-87	33.574096004143684	35.0	33.0	35.0	29.0	36.5
88-89	33.43953508667016	35.0	33.0	35.0	29.0	36.0
90-91	33.33774821208975	35.0	33.0	35.0	29.0	36.0
92-93	33.27029292547483	35.0	33.0	35.0	29.0	36.0
94-95	33.24135880476168	35.0	33.0	35.0	29.0	35.0
96-97	33.26093389728996	35.0	33.0	35.0	29.0	35.0
98-99	33.31771624678226	35.0	33.0	35.0	29.0	35.0
100	33.33024680811889	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	46.0
20	208.0
21	1279.0
22	5965.0
23	19930.0
24	53070.0
25	113325.0
26	205143.0
27	325630.0
28	469089.0
29	626453.0
30	800504.0
31	1004164.0
32	1265177.0
33	1622976.0
34	2207609.0
35	3144296.0
36	5024181.0
37	8954602.0
38	1.1328177E7
39	5882625.0
40	351469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.473348972519304	19.488839369279653	13.120526748364423	24.917284909836617
2	15.493635949470033	21.20876815780334	39.30509026129497	23.992505631431655
3	17.94228852961297	26.28685357123167	30.021237126983202	25.74962077217216
4	12.685934297816123	17.884072690170125	38.17677282944143	31.253220182572317
5	13.434332922699893	37.860795355736705	35.14604103142337	13.558830690140027
6	30.56251091300287	37.77021078916455	18.419960065695385	13.247318232137193
7	27.114633045612297	32.105629989639404	23.123324691711254	17.656412273037045
8	25.552873254054603	35.24699220210144	20.852215452153132	18.347919091690823
9	26.40349214997722	15.559617724999525	20.281758769108134	37.755131355915125
10-11	24.619906175240242	25.897145895701897	28.772790381884946	20.71015754717292
12-13	25.709697466417314	23.629628904348888	28.538055576514036	22.122618052719762
14-15	22.917890817901014	24.75386943141979	25.890875496835974	26.437364253843228
16-17	22.67356357455808	27.75216977866501	26.485255181176303	23.089011465600606
18-19	22.82706335598193	27.02485087001371	27.939682197764476	22.20840357623988
20-21	23.584168365911264	26.236530124963238	27.727300107544618	22.452001401580883
22-23	23.310026946329792	26.08252159690427	27.638645528361387	22.968805928404546
24-25	23.047660554814456	26.377695042881914	27.639746506711855	22.934897895591778
26-27	23.00701607104863	26.4441121078366	27.611340506058912	22.937531315055864
28-29	22.967570265577976	26.3083827923764	27.76811328420445	22.95593365784117
30-31	23.212793399255887	26.293718206752796	27.556354597236233	22.93713379675508
32-33	23.014488548888508	26.39966370668858	27.531507276328167	23.054340468094747
34-35	23.21348933852237	26.40544726193184	27.547537616747032	22.83352578279876
36-37	23.258526531357056	26.334809858080916	27.40816250519142	22.998501105370607
38-39	23.0994530439494	26.436675318715746	27.484612058809837	22.979259578525014
40-41	23.20858119543523	26.343195901017115	27.501212933221268	22.94700997032639
42-43	23.240326318436978	26.416425962537755	27.225946682185274	23.117301036839994
44-45	23.18409379443315	26.52745630861518	27.030579415003274	23.2578704819484
46-47	23.339843644036602	26.542702872767933	26.81609468982845	23.30135879336701
48-49	23.363415319968222	26.688415376526724	26.655420485206744	23.29274881829831
50-51	23.267401778345683	26.760952178908788	26.565633365502645	23.40601267724289
52-53	23.41233631388041	26.604740732335696	26.58703111140807	23.395891842375825
54-55	23.45104268250607	26.58811550573535	26.63146808081571	23.32937373094287
56-57	23.40886080710191	26.60903584958491	26.56845312225332	23.41365022105986
58-59	23.341426161326083	26.5899890969662	26.63017992882147	23.43840481288625
60-61	23.45492738896644	26.660378216563714	26.555827548758636	23.32886684571121
62-63	23.33764249823514	26.732304087214025	26.59241734381197	23.337636070738867
64-65	23.41891582666267	26.553268610460577	26.539787774006037	23.488027788870717
66-67	23.45269967812161	26.66655264817251	26.440380546000775	23.44036712770511
68-69	23.408410262153403	26.631462937670292	26.51235874105984	23.447768059116473
70-71	23.43819796715246	26.453924282980022	26.587145042210764	23.52073270765675
72-73	23.4365975567663	26.57201608284004	26.459061257492557	23.5323251029011
74-75	23.349232168763653	26.628155811992038	26.484352771234605	23.53825924800971
76-77	23.47989314653546	26.590216881477797	26.48680928876372	23.443080683223023
78-79	23.441873405900008	26.558653047543768	26.42226089688596	23.577212649670265
80-81	23.39035623655391	26.644604068739646	26.426237910220372	23.538801784486065
82-83	23.50544183783211	26.60169061570816	26.42961629682294	23.46325124963679
84-85	23.441020721120527	26.60645740676583	26.42410974739171	23.528412124721928
86-87	23.36123181543712	26.660499419901377	26.517772818077447	23.46049594658406
88-89	23.43638387635487	26.555984869230414	26.4691785327065	23.538452721708218
90-91	23.499202873105318	26.603470872516606	26.44967725644053	23.447648997937552
92-93	23.463668694698637	26.649088451547986	26.414040388748244	23.473202465005137
94-95	23.308494670739307	26.553267769582224	26.555208471297608	23.583029088380858
96-97	23.4866236007366	26.729691605332604	26.487081278310704	23.296603515620088
98-99	24.544357268533258	28.62315230691475	26.71061309344296	20.121877331109037
100	23.488064121144074	29.54206615775794	20.379135777646496	26.59073394345149
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7833.0
1	7942.5
2	10736.0
3	13676.5
4	18379.5
5	32883.0
6	44761.0
7	50964.5
8	52714.5
9	47580.5
10	39228.5
11	30228.5
12	30560.0
13	43449.5
14	79237.5
15	131039.5
16	172234.5
17	193148.5
18	194268.0
19	178775.0
20	154578.0
21	131051.5
22	117946.5
23	123542.5
24	145923.0
25	183791.0
26	237312.0
27	300286.0
28	367690.5
29	444241.0
30	526420.5
31	608784.0
32	707729.0
33	814051.5
34	911457.5
35	1008703.5
36	1118505.0
37	1218461.0
38	1298469.5
39	1367662.0
40	1421011.5
41	1462684.0
42	1500504.5
43	1561245.0
44	1630453.0
45	1694713.0
46	1741318.0
47	1776200.0
48	1825272.0
49	1854875.0
50	1857586.0
51	1840040.0
52	1811748.0
53	1774793.5
54	1712558.5
55	1630459.5
56	1536837.5
57	1427415.0
58	1306390.0
59	1166514.0
60	1005113.0
61	837774.5
62	684772.0
63	547703.5
64	425227.5
65	326122.5
66	244947.0
67	186157.0
68	144370.5
69	111910.0
70	86534.5
71	67036.5
72	52446.5
73	41131.0
74	30395.0
75	21891.5
76	15515.5
77	10355.5
78	7055.5
79	4750.5
80	3205.0
81	2264.5
82	1694.0
83	1243.5
84	917.0
85	681.5
86	486.0
87	356.0
88	243.0
89	151.5
90	104.0
91	72.5
92	51.5
93	31.0
94	18.0
95	14.0
96	13.0
97	11.5
98	8.0
99	9.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.002944298503026949
4	2.303832944465531E-6
5	0.11178427829841202
6	3.133212804473122E-4
7	2.4881395800227735E-4
8	0.0
9	0.0
10-11	0.013025871468008111
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.4461586982539E-5
22-23	1.0369112166085065E-5
24-25	4.0678422515665893E-4
26-27	0.0
28-29	3.0795566434964057E-4
30-31	0.0
32-33	6.017301756030329E-5
34-35	8.515595896225282E-4
36-37	9.430655823057984E-4
38-39	2.691895674717038E-4
40-41	0.0022495026832444888
42-43	0.001299153613274543
44-45	0.0018253002938530397
46-47	5.196229582057851E-4
48-49	0.005153247698446102
50-51	0.0018994656836140821
52-53	4.0771250723349936E-4
54-55	4.430188694554182E-4
56-57	5.392633912895168E-5
58-59	0.003617145172029073
60-61	0.0026951959661815376
62-63	0.002640345753147828
64-65	5.791905991483152E-4
66-67	8.178952662116478E-4
68-69	0.004166548750656865
70-71	0.0022855947794780196
72-73	0.0
74-75	0.0012314095428902112
76-77	0.0011467883011300416
78-79	1.1591622022707406E-4
80-81	2.7881809844523362E-6
82-83	6.33936113130041E-5
84-85	2.561461704382957E-5
86-87	9.143251723869542E-4
88-89	9.156624852963499E-5
90-91	0.0010298310876934716
92-93	0.0012299083293417217
94-95	4.053826588431444E-4
96-97	1.2039664024840235E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1169.0
20-21	4660.0
22-23	8702.0
24-25	11812.0
26-27	20383.0
28-29	41310.0
30-31	84984.0
32-33	104274.0
34-35	137630.0
36-37	204656.0
38-39	266848.0
40-41	266660.0
42-43	306586.0
44-45	377818.0
46-47	371450.0
48-49	342537.0
50-51	313311.0
52-53	288158.0
54-55	304064.0
56-57	319945.0
58-59	327271.0
60-61	327834.0
62-63	308195.0
64-65	314660.0
66-67	332618.0
68-69	345933.0
70-71	351972.0
72-73	330763.0
74-75	325715.0
76-77	341778.0
78-79	362183.0
80-81	376190.0
82-83	359973.0
84-85	353435.0
86-87	370661.0
88-89	404163.0
90-91	441432.0
92-93	469517.0
94-95	561487.0
96-97	1515051.0
98-99	2778761.0
100-101	2.8329376E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.5102201207587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.01832398605974	67.53317550564289
2	9.15613539192615	14.377004101511822
3	2.414113629227324	5.685977772814826
4	0.9876687158131465	3.101683531395088
5	0.5224462012843238	2.0508683132043237
6	0.28700416622949476	1.351965615975147
7	0.16722630967424892	0.9190282067755209
8	0.10686455517686762	0.6711967800034272
9	0.07799166686505934	0.5510828639844623
>10	0.254371997757661	3.045951986970408
>50	0.005619870287945203	0.3024706828162983
>100	0.0021244834960322595	0.283299170906699
>500	5.152725729331213E-5	0.02767366008899362
>1k	5.749894467514451E-5	0.0986218079101373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.303832944465531E-6
2	0.0	1.6126830611258716E-5	0.0	0.0	2.303832944465531E-6
3	2.303832944465531E-6	1.6126830611258716E-5	0.0	0.0	2.303832944465531E-6
4	2.303832944465531E-6	6.450732244503486E-5	0.0	4.607665888931062E-6	2.303832944465531E-6
5	6.911498833396593E-6	9.445715072308677E-5	0.0	6.911498833396593E-6	2.303832944465531E-6
6	6.911498833396593E-6	9.445715072308677E-5	0.0	1.6126830611258716E-5	1.1519164722327654E-5
7	9.215331777862124E-6	1.0136864955648336E-4	2.303832944465531E-6	2.073449650018978E-5	1.1519164722327654E-5
8	9.215331777862124E-6	1.1288781427881101E-4	2.303832944465531E-6	2.3038329444655308E-5	1.3822997666793186E-5
9	9.215331777862124E-6	1.8891430144617354E-4	2.303832944465531E-6	4.6076658889310615E-5	1.6126830611258716E-5
10-11	3.110174475028467E-5	1.9812963322403567E-4	2.303832944465531E-6	7.141882127843145E-5	1.6126830611258716E-5
12-13	4.9532408306008916E-5	2.0504113205743225E-4	2.303832944465531E-6	8.524181894522466E-5	2.3038329444655308E-5
14-15	5.529199066717274E-5	2.488139580022773E-4	3.4557494166982964E-6	9.906481661201783E-5	2.764599533358637E-5
16-17	5.9899656556103806E-5	2.568773733079067E-4	4.607665888931062E-6	1.1749548016774209E-4	3.225366122251743E-5
18-19	5.9899656556103806E-5	2.7991570275256203E-4	4.607665888931062E-6	1.3016656136230248E-4	4.146899300037956E-5
20-21	6.335540597280211E-5	2.9949828278051904E-4	4.607665888931062E-6	1.3938189314016464E-4	4.377282594484509E-5
22-23	6.565923891726763E-5	3.248404451696399E-4	4.607665888931062E-6	1.4859722491802674E-4	4.7228575361543385E-5
24-25	7.257073775066423E-5	3.490306910865279E-4	4.607665888931062E-6	1.8200280261277695E-4	5.068432477824168E-5
26-27	7.372265422289699E-5	3.674613546422522E-4	4.607665888931062E-6	2.2923137797432031E-4	5.068432477824168E-5
28-29	7.717840363959528E-5	3.7437285347564876E-4	4.607665888931062E-6	5.056913313101841E-4	5.183624125047444E-5
30-31	7.948223658406082E-5	4.2275334530942493E-4	4.607665888931062E-6	0.0013396788572067062	5.529199066717274E-5
32-33	8.293798600075912E-5	4.296648441428215E-4	4.607665888931062E-6	0.0029339312547768536	5.7595823611638274E-5
34-35	8.524181894522466E-5	4.907164171711581E-4	4.607665888931062E-6	0.005016596236573694	6.450732244503486E-5
36-37	8.869756836192295E-5	5.517679901994947E-4	4.607665888931062E-6	0.008170543537547006	6.450732244503486E-5
38-39	8.98494848341557E-5	5.701986537552189E-4	4.607665888931062E-6	0.013602981620596727	6.565923891726763E-5
40-41	9.215331777862123E-5	6.53136639755978E-4	4.607665888931062E-6	0.019915483888432284	7.602648716736252E-5
42-43	9.445715072308677E-5	6.738711362561677E-4	4.607665888931062E-6	0.025698104579040766	7.717840363959528E-5
44-45	9.560906719531954E-5	7.268592939788749E-4	4.607665888931062E-6	0.03206474692107126	8.293798600075912E-5
46-47	1.036724825009489E-4	7.648725375625563E-4	4.607665888931062E-6	0.03940015101624951	8.524181894522464E-5
48-49	1.0482439897318167E-4	7.740878693404184E-4	4.607665888931062E-6	0.04673209936201106	9.215331777862123E-5
50-51	1.0597631544541442E-4	8.570258553411774E-4	4.607665888931062E-6	0.0542817599210246	9.445715072308677E-5
52-53	1.1058398133434549E-4	8.650892706468068E-4	4.607665888931062E-6	0.06149390895367395	1.0367248250094888E-4
54-55	1.1403973075104378E-4	8.89279516563695E-4	4.607665888931062E-6	0.06857473950848876	1.1173589780657825E-4
56-57	1.197993131122076E-4	9.031025142304881E-4	4.607665888931062E-6	0.07564059514916455	1.2095122958444037E-4
58-59	1.2210314605667314E-4	9.272927601473762E-4	4.607665888931062E-6	0.08225950719861402	1.3016656136230248E-4
60-61	1.2440697900113868E-4	9.411157578141695E-4	4.607665888931062E-6	0.08896481298348094	1.3822997666793185E-4
62-63	1.267108119456042E-4	9.560906719531953E-4	4.607665888931062E-6	0.09511143927931498	1.4283764255686293E-4
64-65	1.267108119456042E-4	0.0010620669873986099	4.607665888931062E-6	0.1007166648331996	1.4514147550132844E-4
66-67	1.2786272841783697E-4	0.0010920168156766615	4.607665888931062E-6	0.10616062208097166	1.4514147550132844E-4
68-69	1.3131847783453526E-4	0.001134637725149274	4.607665888931062E-6	0.11137304411782492	1.5090105786249227E-4
70-71	1.3131847783453526E-4	0.0011588279710661622	4.607665888931062E-6	0.11604291349625656	1.566606402236561E-4
72-73	1.3131847783453526E-4	0.0011703471357884898	4.607665888931062E-6	0.11943530750698206	1.7278747083491482E-4
74-75	1.336223107790008E-4	0.0011922335487609122	4.607665888931062E-6	0.12219875512386846	1.773951367238459E-4
76-77	1.336223107790008E-4	0.0012290948758723608	4.607665888931062E-6	0.12467883128858559	1.831547190850097E-4
78-79	1.405338096123974E-4	0.0012555889547337144	4.607665888931062E-6	0.12630764118032273	1.8430663555724246E-4
80-81	1.4398955902909569E-4	0.0012636523700393437	4.607665888931062E-6	0.12689972624705037	1.9237005086287183E-4
82-83	1.4744530844579398E-4	0.0012659562029838092	4.607665888931062E-6	0.12728216251583163	2.096487979463633E-4
84-85	1.4744530844579398E-4	0.0012717157853449732	4.607665888931062E-6	0.12742269632544406	2.096487979463633E-4
86-87	1.4744530844579398E-4	0.0012993617806785595	4.607665888931062E-6	0.12747453256669453	2.096487979463633E-4
88	1.4744530844579398E-4	0.001375388267845922	4.607665888931062E-6	0.12748259598200015	2.1425646383529438E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	49080	0.0	50.277847	1
GTATCAA	89615	0.0	37.840504	1
TCAACGC	110140	0.0	30.055754	4
ATCAACG	110750	0.0	29.905066	3
CAACGCA	112575	0.0	29.408295	5
AACGCAG	116985	0.0	28.338636	6
TATCAAC	118895	0.0	28.12248	2
ACGCAGA	134590	0.0	24.574572	7
CGCAGAG	139260	0.0	23.750422	8
GTACATG	140000	0.0	22.881403	1
TACATGG	143335	0.0	21.9046	2
ACATGGG	144485	0.0	21.123678	3
GCAGAGT	165650	0.0	19.961544	9
AGAGTAC	145775	0.0	18.674313	10-11
GAGTACT	83860	0.0	18.172	12-13
CAGAGTA	157745	0.0	18.037075	10-11
CATGGGG	94745	0.0	17.44266	4
GTACTTT	93485	0.0	16.235828	14-15
AGTACTT	94190	0.0	15.909278	12-13
GTGGTAT	24480	0.0	14.495112	1
>>END_MODULE