FastQCFastQC Report
Fri 27 May 2016
SRR2140404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19948986
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208850.049.650131
GTATCAA398400.038.621381
TCAACGC485450.030.8875874
ATCAACG485000.030.849183
CAACGCA495950.030.2364585
AACGCAG518750.029.0238426
TATCAAC526300.028.6730212
ACGCAGA589500.025.496657
CGCAGAG609650.024.6750438
GTACATG604150.021.923461
TACATGG616250.021.0611862
GCAGAGT723550.020.7433269
ACATGGG621250.020.3044473
AGAGTAC644800.018.97195410-11
CAGAGTA692800.018.05360210-11
GAGTACT372350.017.97227512-13
CATGGGG416950.017.2860834
GTACTTT421250.015.82028514-15
AGTACTT421850.015.81767412-13
GTGGTAT114600.014.718581
Produced by FastQC (version 0.11.5)