##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140404_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19948986
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.369374714083214	33.0	31.0	34.0	31.0	34.0
2	32.44035320893002	34.0	31.0	34.0	31.0	34.0
3	32.47854186673949	34.0	31.0	34.0	31.0	34.0
4	36.06094064129375	37.0	35.0	37.0	35.0	37.0
5	35.972144549101394	37.0	35.0	37.0	35.0	37.0
6	36.07097648973236	37.0	35.0	37.0	35.0	37.0
7	36.01404261850703	37.0	35.0	37.0	35.0	37.0
8	36.01265628237947	37.0	35.0	37.0	35.0	37.0
9	37.73831326564669	39.0	38.0	39.0	35.0	39.0
10-11	37.67976414941592	39.0	37.0	39.0	35.0	39.0
12-13	37.6645354806505	39.0	37.0	39.0	35.0	39.0
14-15	39.06256230266541	40.0	38.0	41.0	36.0	41.0
16-17	39.06809100472576	40.0	38.0	41.0	36.0	41.0
18-19	39.09401718463285	40.5	38.5	41.0	36.0	41.0
20-21	39.10019191721496	40.0	39.0	41.0	35.0	41.0
22-23	39.056743486817055	40.0	39.0	41.0	35.0	41.0
24-25	39.02684892560953	40.0	38.5	41.0	35.0	41.0
26-27	38.93795993985502	40.0	38.0	41.0	35.0	41.0
28-29	38.872725448765706	40.0	38.0	41.0	35.0	41.0
30-31	38.79973317260029	40.0	38.0	41.0	35.0	41.0
32-33	38.71007714299559	40.0	38.0	41.0	34.5	41.0
34-35	38.64766125779134	40.0	38.0	41.0	34.0	41.0
36-37	38.60330540373181	40.0	38.0	41.0	34.0	41.0
38-39	38.55379429861932	40.0	38.0	41.0	34.0	41.0
40-41	38.50756060704496	40.0	38.0	41.0	34.0	41.0
42-43	38.44687469048216	40.0	38.0	41.0	34.0	41.0
44-45	38.376756800399306	40.0	38.0	41.0	33.5	41.0
46-47	38.300715145407935	40.0	38.0	41.0	33.0	41.0
48-49	38.21119449438645	40.0	37.5	41.0	33.0	41.0
50-51	38.10182019295224	40.0	37.0	41.0	33.0	41.0
52-53	37.984372222398896	40.0	37.0	41.0	33.0	41.0
54-55	37.82436003265836	40.0	36.5	41.0	33.0	41.0
56-57	37.668574829079674	39.5	36.0	41.0	33.0	41.0
58-59	37.4706621352708	39.0	36.0	41.0	32.0	41.0
60-61	37.26056309967646	39.0	35.0	41.0	32.0	41.0
62-63	37.009047666048644	38.5	35.0	40.5	32.0	41.0
64-65	36.74614748528813	38.0	35.0	40.0	31.0	41.0
66-67	36.465100110415364	37.0	35.0	40.0	31.0	41.0
68-69	36.151047290112444	37.0	35.0	39.5	31.0	41.0
70-71	35.83070792725361	36.0	34.5	39.0	31.0	41.0
72-73	35.50067375031056	36.0	34.0	39.0	31.0	40.5
74-75	35.172614766070666	35.0	34.0	38.0	30.0	40.0
76-77	34.86898002568386	35.0	34.0	37.0	30.0	39.0
78-79	34.568181429573926	35.0	34.0	37.0	30.0	39.0
80-81	34.30593764411914	35.0	34.0	36.0	30.0	39.0
82-83	34.07021864401031	35.0	34.0	36.0	30.0	37.0
84-85	33.859009688233684	35.0	34.0	35.5	30.0	37.0
86-87	33.69224490974053	35.0	33.0	35.0	30.0	37.0
88-89	33.55409945222445	35.0	33.0	35.0	29.0	36.0
90-91	33.44982157812642	35.0	33.0	35.0	29.0	36.0
92-93	33.38029834447106	35.0	33.0	35.0	29.0	36.0
94-95	33.346908961326776	35.0	33.0	35.0	29.0	35.5
96-97	33.3618153923474	35.0	33.0	35.0	30.0	35.0
98-99	33.41029696522792	35.0	33.0	35.0	30.0	35.0
100	33.42198200617698	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	18.0
20	94.0
21	531.0
22	2417.0
23	8370.0
24	22172.0
25	48210.0
26	87348.0
27	139685.0
28	200972.0
29	268436.0
30	346218.0
31	434180.0
32	549449.0
33	708416.0
34	963230.0
35	1380870.0
36	2221527.0
37	4083210.0
38	5413031.0
39	2894625.0
40	175971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22348444176561	19.587120869201073	13.386008692371632	24.803385996661685
2	15.2962511478027	21.148834331729944	39.74329321801118	23.811621302456174
3	17.889279340699005	26.263610114094366	30.080051532955025	25.767059012251607
4	12.66088602110694	17.99868193782109	38.40750678869193	30.932925252380034
5	13.47484662576687	37.79513969914813	35.20283036621626	13.527183308868732
6	30.324804550822805	37.874837334552446	18.573742337931076	13.226615776693675
7	26.93918459570204	32.16361121395708	23.196822726195887	17.70038146414499
8	25.25187495745398	35.5080654224731	20.922065913525632	18.317993706547288
9	26.179200286169934	15.854896083440032	20.501693670044183	37.464209960345855
10-11	24.474413669813064	26.00889887161189	28.876595480356254	20.640091978218795
12-13	25.56725690218039	23.69244481899982	28.623753107050153	22.116545171769634
14-15	22.83227578584696	24.880633030671333	25.96866577579432	26.31842540768739
16-17	22.599253916966006	27.85997243168149	26.57377422591805	22.966999425434455
18-19	22.703402067653965	27.19959300186987	27.97979305815343	22.117211872322734
20-21	23.48569102968957	26.39175740427043	27.779937406173865	22.342614159866134
22-23	23.215807564186235	26.220211882044648	27.675001726556776	22.888978827212338
24-25	22.98131305928588	26.479549540496244	27.689709651233667	22.8494277489842
26-27	22.935107931574944	26.582495087749376	27.640014002541385	22.842382978134296
28-29	22.899929706177442	26.439844605308583	27.806851556173946	22.853374132340036
30-31	23.132362134787808	26.403260558132047	27.59965185676184	22.864725450318304
32-33	22.925024145283885	26.570943761595455	27.555373045926146	22.94865904719451
34-35	23.128737000777658	26.531460343426577	27.565025545486364	22.7747771103094
36-37	23.192270366864243	26.44075091505977	27.454424550351725	22.91255416772426
38-39	23.02161417975198	26.554622973986476	27.53987926049849	22.88388358576305
40-41	23.117432832309916	26.461732392308445	27.527790294313093	22.893044481068543
42-43	23.1618339772629	26.529634515690336	27.26458388739698	23.04394761964978
44-45	23.106160209999928	26.65435390805426	27.100441438756622	23.13904444318919
46-47	23.26100264831781	26.6509484947006	26.888421653261048	23.199627203720542
48-49	23.26237265051158	26.765500720923203	26.766179914671067	23.20594671389415
50-51	23.216485055323773	26.832785244805667	26.659186513050763	23.291543186819794
52-53	23.339994183996428	26.732716592273707	26.63611856265888	23.291170661070986
54-55	23.36836611340015	26.660015696045708	26.70850657809561	23.263111612458538
56-57	23.308845606186935	26.739313227591865	26.651845004337616	23.299996161883584
58-59	23.273001648152952	26.697724008259254	26.712705471133674	23.31656887245412
60-61	23.3990610712908	26.75713716363251	26.628511943969883	23.215289821106808
62-63	23.25642422899456	26.856827343446945	26.6533108969631	23.2334375305954
64-65	23.326708994365475	26.687093271924528	26.605619182021815	23.38057855168818
66-67	23.35425268125476	26.7578679243355	26.534524029000227	23.353355365409517
68-69	23.320579285381093	26.744626815997417	26.57612003414896	23.358673864472536
70-71	23.357452139876216	26.58355009810498	26.640488827574195	23.418508934444606
72-73	23.366986292650687	26.67968504090725	26.524574866144015	23.428753800298054
74-75	23.247744387965533	26.749450206423646	26.56820781929098	23.434597586319843
76-77	23.399942313852833	26.710867100810088	26.517580089768156	23.371610495568923
78-79	23.36458612328172	26.650180441969873	26.4973704240982	23.48786301065021
80-81	23.299349005693955	26.76762583487437	26.497344569452036	23.435680589979643
82-83	23.409778401704674	26.715218005832792	26.49829865536218	23.376704937100353
84-85	23.366628368133654	26.70928084739251	26.484537980647815	23.43955280382602
86-87	23.303059165212435	26.779716772514305	26.58151409541526	23.335709966857994
88-89	23.372593232179888	26.640389433595402	26.546189115982305	23.440828218242405
90-91	23.40356282049929	26.712581789026103	26.520467271756704	23.36338811871791
92-93	23.39304225940845	26.755090882882005	26.476498097971866	23.37536875973768
94-95	23.211410055611484	26.666257181892583	26.639595100934578	23.482737661561355
96-97	23.39671590780487	26.820775465786255	26.58391221622956	23.198596410179313
98-99	24.45552733058679	28.744785181046893	26.78043354844606	20.019253939920254
100	23.384050904798727	29.64939447187514	20.479264909195788	26.48728971413034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2550.0
1	2648.0
2	4279.5
3	5864.0
4	7998.0
5	14559.5
6	19823.5
7	22375.5
8	22852.0
9	20405.0
10	16592.5
11	12651.0
12	13035.0
13	18981.0
14	34699.5
15	56955.0
16	74692.5
17	84126.5
18	84791.0
19	78187.0
20	68011.0
21	58262.5
22	53284.0
23	56524.5
24	67896.5
25	86818.0
26	112922.0
27	143385.0
28	175188.5
29	211612.0
30	252021.0
31	291305.0
32	336333.0
33	384298.0
34	429115.5
35	473202.0
36	521893.5
37	566756.0
38	603130.5
39	635308.5
40	659357.0
41	679242.0
42	696822.5
43	724302.0
44	756876.5
45	784695.5
46	804138.0
47	819166.0
48	840110.0
49	852173.0
50	851616.5
51	842104.5
52	826650.5
53	803807.0
54	771601.0
55	734169.5
56	690874.0
57	640489.5
58	584022.0
59	516908.0
60	442303.0
61	369251.0
62	301916.5
63	240712.0
64	186293.5
65	142781.0
66	107617.5
67	81379.5
68	62443.0
69	48000.5
70	37309.5
71	29044.5
72	22621.5
73	17618.5
74	12908.0
75	9060.0
76	6375.5
77	4354.0
78	2966.0
79	1941.5
80	1300.5
81	932.5
82	694.0
83	505.0
84	373.0
85	283.5
86	216.5
87	162.5
88	110.5
89	75.5
90	54.0
91	36.5
92	24.0
93	12.0
94	4.0
95	5.0
96	7.0
97	6.0
98	4.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0029374926625343263
4	1.5038358340619417E-5
5	0.11146431202067114
6	3.308438834936272E-4
7	3.0076716681238835E-4
8	0.0
9	0.0
10-11	0.01265477854363124
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.268845208086486E-5
22-23	1.2533985902775375E-5
24-25	3.7859748786275377E-4
26-27	0.0
28-29	3.061371541123793E-4
30-31	0.0
32-33	5.538050421482138E-5
34-35	7.975132325995043E-4
36-37	8.484311659310769E-4
38-39	2.2147635322433676E-4
40-41	0.0021156424506884983
42-43	0.0012423587206158964
44-45	0.0017452076325720877
46-47	5.161977617036182E-4
48-49	0.0049523192604311065
50-51	0.0018059013500018207
52-53	4.104829291417929E-4
54-55	4.592941880319065E-4
56-57	8.166198471287646E-5
58-59	0.0035333166799266626
60-61	0.0028231714185996725
62-63	0.0026447380998629697
64-65	6.73961665172812E-4
66-67	9.284443679672993E-4
68-69	0.004324525402604752
70-71	0.002364231827530757
72-73	0.0
74-75	0.0013682574353804534
76-77	0.0012057205054758625
78-79	1.3420065526899508E-4
80-81	0.0
82-83	9.731952527413923E-5
84-85	2.1495323400671938E-5
86-87	9.427058722327163E-4
88-89	9.087544420465513E-5
90-91	9.954020982252012E-4
92-93	0.0011621675799420405
94-95	4.5895936530021114E-4
96-97	1.2943330217309555E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	477.0
20-21	1884.0
22-23	3640.0
24-25	4941.0
26-27	8615.0
28-29	17508.0
30-31	38431.0
32-33	47621.0
34-35	62778.0
36-37	92891.0
38-39	119311.0
40-41	120147.0
42-43	137850.0
44-45	168533.0
46-47	165345.0
48-49	152060.0
50-51	138596.0
52-53	128402.0
54-55	136113.0
56-57	141596.0
58-59	143228.0
60-61	143430.0
62-63	136076.0
64-65	139489.0
66-67	145959.0
68-69	152079.0
70-71	154020.0
72-73	146428.0
74-75	144887.0
76-77	151200.0
78-79	159778.0
80-81	164710.0
82-83	159270.0
84-85	157452.0
86-87	164867.0
88-89	178303.0
90-91	196066.0
92-93	206871.0
94-95	249106.0
96-97	688903.0
98-99	1284041.0
100-101	1.3196084E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.69203012658227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.69460959341119	71.63950688843816
2	8.386632596542592	13.702420854746686
3	2.1573488692769933	5.287146264675731
4	0.8049046868756549	2.6301719171709306
5	0.35963469327413367	1.468964409875735
6	0.20399459811611068	0.9998839712976814
7	0.12087974714781954	0.6912438361985324
8	0.07501520137113304	0.49025152722897886
9	0.0443792527669065	0.3262888128626401
>10	0.14544246519506268	1.819648289135264
>50	0.003976726112465879	0.2190555276979527
>100	0.0027899309778561085	0.45553705839463043
>500	2.937904931033469E-4	0.163271076542002
>1k	9.7848438993694E-5	0.10660956573505158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.012786113539806E-6
2	0.0	1.0025572227079612E-5	0.0	0.0	5.012786113539806E-6
3	0.0	1.5038358340619417E-5	0.0	2.0051144454159224E-5	5.012786113539806E-6
4	0.0	2.0051144454159224E-5	0.0	2.506393056769903E-5	5.012786113539806E-6
5	0.0	2.506393056769903E-5	0.0	2.506393056769903E-5	5.012786113539806E-6
6	9.023015004371651E-5	2.506393056769903E-5	0.0	2.506393056769903E-5	1.0025572227079612E-5
7	9.023015004371651E-5	2.506393056769903E-5	0.0	2.506393056769903E-5	1.0025572227079612E-5
8	9.524293615725631E-5	2.506393056769903E-5	0.0	3.0076716681238835E-5	1.5038358340619417E-5
9	1.0025572227079612E-4	7.519179170309709E-5	0.0	6.516621947601747E-5	4.511507502185826E-5
10-11	1.1028129449787574E-4	8.02045778166369E-5	0.0	1.0025572227079612E-4	4.7621468078628155E-5
12-13	1.1028129449787574E-4	9.774932921402621E-5	0.0	1.1780047366818545E-4	5.012786113539806E-5
14-15	1.2531965283849516E-4	1.3283883200880487E-4	0.0	1.2782604589526507E-4	7.519179170309709E-5
16-17	1.9800505148482233E-4	1.4035801117911456E-4	0.0	1.4035801117911456E-4	8.02045778166369E-5
18-19	2.2557537510929128E-4	1.579027625765039E-4	0.0	1.604091556332738E-4	8.52173639301767E-5
20-21	2.381073403931408E-4	1.854730862009728E-4	0.0	1.9048587231451262E-4	8.52173639301767E-5
22-23	2.606648779040699E-4	2.0552423065513204E-4	0.0	2.1053701676867185E-4	8.772375698694661E-5
24-25	2.757032362446893E-4	2.2306898205252137E-4	0.0	2.356009473363709E-4	9.023015004371651E-5
26-27	3.1079273903946797E-4	2.3560094733637087E-4	0.0	2.9826077375561845E-4	1.0526850838433593E-4
28-29	3.458822418342466E-4	2.3810734039314077E-4	0.0	6.692069461575641E-4	1.0526850838433593E-4
30-31	3.8347813768579514E-4	2.706904501311495E-4	0.0	0.0017193856369441536	1.0526850838433593E-4
32-33	4.060356751967243E-4	2.706904501311495E-4	0.0	0.0039225051338448984	1.1529408061141554E-4
34-35	4.185676404805738E-4	3.233247043233175E-4	0.0	0.006844959438038605	1.3033243895203494E-4
36-37	4.235804265941136E-4	3.8097174462902524E-4	0.0	0.01109580206232036	1.3785161812234466E-4
38-39	4.260868196508835E-4	4.110484613102641E-4	0.0	0.017923216748961576	1.4035801117911456E-4
40-41	4.260868196508835E-4	4.63682715502432E-4	0.0	0.025417331988703586	1.4537079729265437E-4
42-43	4.611763224456622E-4	5.087977905242903E-4	0.0	0.03271594857001754	1.4787719034942427E-4
44-45	4.7120189467274174E-4	5.66444830829998E-4	0.0	0.04046070311543654	1.5288997646296408E-4
46-47	4.8624025301336116E-4	6.040407266815467E-4	0.0	0.04902003540430576	1.604091556332738E-4
48-49	4.887466460701311E-4	6.140662989086263E-4	0.0	0.057767347172432724	1.7795390703066312E-4
50-51	5.062913974675205E-4	6.892580906117233E-4	0.0	0.06645199911414043	1.8046030008743303E-4
52-53	5.163169696946001E-4	6.942708767252632E-4	0.0	0.07510406794611013	1.9048587231451262E-4
54-55	5.163169696946001E-4	7.068028420091127E-4	0.0	0.08265583022615786	2.0301783759836214E-4
56-57	5.163169696946001E-4	7.268539864632718E-4	0.0	0.0887563909263358	2.1304340982544176E-4
58-59	5.163169696946001E-4	7.318667725768116E-4	0.0	0.0950449311057715	2.1554980288221166E-4
60-61	5.463936863758388E-4	7.46905130917431E-4	0.0	0.10125827949350408	2.2056258899575147E-4
62-63	5.814831891706175E-4	7.569307031445107E-4	0.0	0.10719592464499197	2.2557537510929128E-4
64-65	5.864959752841573E-4	8.070585642799088E-4	0.0	0.11285285377412166	2.3560094733637087E-4
66-67	5.990279405680067E-4	8.471608531882272E-4	0.0	0.11831679063788005	2.3560094733637087E-4
68-69	6.015343336247767E-4	8.596928184720768E-4	0.0	0.12368548456548117	2.3560094733637087E-4
70-71	6.065471197383165E-4	8.772375698694661E-4	0.0	0.1283949971191518	2.4061373344991068E-4
72-73	6.491558017034048E-4	8.972887143236253E-4	0.0	0.13190394739862968	2.656776640176097E-4
74-75	6.516621947601748E-4	9.27365431004864E-4	0.0	0.13444542995819436	2.706904501311495E-4
76-77	6.541685878169448E-4	9.79999685197032E-4	0.0	0.13695432940802105	2.782096293014592E-4
78-79	6.591813739304845E-4	9.925316504808817E-4	0.0	0.13859601686020534	2.8572880847176893E-4
80-81	6.71713339214334E-4	0.0010025572227079613	0.0	0.13921760233828426	2.8823520152853883E-4
82-83	6.71713339214334E-4	0.001010076401878271	0.0	0.13959356129679973	2.9324798764207864E-4
84-85	6.71713339214334E-4	0.0010175955810485808	0.0	0.13969632341212732	2.9826077375561845E-4
86-87	6.74219732271104E-4	0.0010426595116162795	0.0	0.13971637455658148	3.0076716681238835E-4
88	6.867516975549535E-4	0.001087774586638138	0.0	0.13972640012880855	3.1079273903946797E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20885	0.0	49.65013	1
GTATCAA	39840	0.0	38.62138	1
TCAACGC	48545	0.0	30.887587	4
ATCAACG	48500	0.0	30.84918	3
CAACGCA	49595	0.0	30.236458	5
AACGCAG	51875	0.0	29.023842	6
TATCAAC	52630	0.0	28.673021	2
ACGCAGA	58950	0.0	25.49665	7
CGCAGAG	60965	0.0	24.675043	8
GTACATG	60415	0.0	21.92346	1
TACATGG	61625	0.0	21.061186	2
GCAGAGT	72355	0.0	20.743326	9
ACATGGG	62125	0.0	20.304447	3
AGAGTAC	64480	0.0	18.971954	10-11
CAGAGTA	69280	0.0	18.053602	10-11
GAGTACT	37235	0.0	17.972275	12-13
CATGGGG	41695	0.0	17.286083	4
GTACTTT	42125	0.0	15.820285	14-15
AGTACTT	42185	0.0	15.817674	12-13
GTGGTAT	11460	0.0	14.71858	1
>>END_MODULE