FastQCFastQC Report
Fri 27 May 2016
SRR2140403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7932645
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108850.054.091641
GTATCAA207250.037.7616041
TCAACGC256050.029.9357014
ATCAACG257400.029.8456153
CAACGCA262600.029.2054185
TATCAAC265650.029.1686882
AACGCAG270600.028.373826
ACGCAGA311050.024.6839927
CGCAGAG313500.024.4910858
TGGTATC47050.022.7032262
GCAGAGT361900.021.2166189
GTGGTAT53600.020.732181
GAGTACT220100.020.17214212-13
TACATGG238350.020.0045152
GTACATG246500.019.9020061
AGAGTAC330150.019.324310-11
ACATGGG245350.018.9553553
CAGAGTA353750.018.9420310-11
GTACTTT242500.018.22042814-15
AGTACTT241350.017.60204712-13
Produced by FastQC (version 0.11.5)