##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7932645
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.918075118702525	34.0	31.0	34.0	31.0	34.0
2	33.028392421443286	34.0	33.0	34.0	31.0	34.0
3	33.090898306932935	34.0	33.0	34.0	31.0	34.0
4	36.46326149726857	37.0	37.0	37.0	35.0	37.0
5	36.42250951605675	37.0	37.0	37.0	35.0	37.0
6	36.45387938071097	37.0	37.0	37.0	35.0	37.0
7	36.414119754508114	37.0	37.0	37.0	35.0	37.0
8	36.397767957598	37.0	37.0	37.0	35.0	37.0
9	38.18379430820363	39.0	39.0	39.0	37.0	39.0
10-11	38.13618307638877	39.0	39.0	39.0	37.0	39.0
12-13	38.10468576370177	39.0	38.0	39.0	37.0	39.0
14-15	39.55852801934286	41.0	40.0	41.0	37.0	41.0
16-17	39.53762244497264	41.0	39.0	41.0	37.0	41.0
18-19	39.54790873661938	41.0	39.5	41.0	37.0	41.0
20-21	39.52439447471107	41.0	39.5	41.0	37.0	41.0
22-23	39.4430502588798	41.0	39.0	41.0	36.5	41.0
24-25	39.287932464614805	40.5	39.0	41.0	36.0	41.0
26-27	39.29204579798484	40.5	39.0	41.0	36.0	41.0
28-29	39.220725471247874	41.0	39.0	41.0	36.0	41.0
30-31	39.13119635113581	40.5	39.0	41.0	36.0	41.0
32-33	38.92977267524405	40.0	39.0	41.0	35.0	41.0
34-35	38.85005123078727	40.0	38.0	41.0	35.0	41.0
36-37	39.03888679454572	40.5	39.0	41.0	35.0	41.0
38-39	39.11167846434519	41.0	39.0	41.0	35.0	41.0
40-41	39.08422847553638	41.0	39.0	41.0	35.0	41.0
42-43	38.93960941129221	40.0	39.0	41.0	35.0	41.0
44-45	38.95930223707494	40.0	39.0	41.0	35.0	41.0
46-47	38.9115155064748	40.0	38.5	41.0	35.0	41.0
48-49	38.84592592322309	40.0	38.0	41.0	35.0	41.0
50-51	38.77517430636399	40.0	38.0	41.0	35.0	41.0
52-53	38.65978052812751	40.0	38.0	41.0	35.0	41.0
54-55	38.52290420695785	40.0	38.0	41.0	34.5	41.0
56-57	38.37755978576845	40.0	37.5	41.0	34.0	41.0
58-59	38.21806807499263	40.0	37.0	41.0	34.0	41.0
60-61	38.03506920024115	40.0	37.0	41.0	34.0	41.0
62-63	37.8047791580893	39.0	36.0	41.0	34.0	41.0
64-65	37.52999582378874	39.0	35.5	41.0	33.0	41.0
66-67	37.206291208322114	38.5	35.0	40.0	33.0	41.0
68-69	36.87384698157975	38.0	35.0	40.0	32.5	41.0
70-71	36.51283642600268	37.0	35.0	40.0	32.0	41.0
72-73	36.16846442869466	37.0	35.0	39.0	32.0	41.0
74-75	35.82457628951248	36.0	35.0	39.0	32.0	40.5
76-77	34.96405700741154	35.0	34.0	38.0	30.5	39.0
78-79	35.19345954374466	35.0	34.0	37.0	31.0	39.0
80-81	34.97716974059556	35.0	34.0	37.0	31.0	39.0
82-83	34.70914181139257	35.0	34.0	36.5	31.0	39.0
84-85	34.44578771162266	35.0	34.0	36.0	31.0	37.0
86-87	34.22824248550119	35.0	34.0	36.0	31.0	37.0
88-89	34.02929608182181	35.0	34.0	35.0	31.0	37.0
90-91	33.8316600779375	35.0	34.0	35.0	31.0	36.0
92-93	33.70053874930356	35.0	34.0	35.0	30.0	36.0
94-95	33.62700142719761	35.0	34.0	35.0	30.0	36.0
96-97	33.60182663393892	35.0	34.0	35.0	30.0	35.5
98-99	33.632331728203766	35.0	34.0	35.0	31.0	35.0
100-101	32.228703794718506	33.5	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	3.0
20	29.0
21	135.0
22	638.0
23	2260.0
24	5752.0
25	12151.0
26	21566.0
27	34561.0
28	50870.0
29	69384.0
30	89967.0
31	116186.0
32	149531.0
33	198052.0
34	288328.0
35	433996.0
36	781115.0
37	1746553.0
38	2575890.0
39	1282667.0
40	73009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.13048411097233	21.049942490801303	15.665873824666782	24.153699573559585
2	13.413892625607984	21.365904073334857	41.925105557060334	23.295097743996827
3	17.105961139816685	26.160546218889948	30.884784442614592	25.84870819867878
4	11.784960978962374	18.566130031802764	39.09029574502524	30.55861324420962
5	13.082145120844954	37.79616228838707	35.55408511966502	13.567607471102953
6	29.73125984224175	38.27264906883506	18.691485461846014	13.304605627077176
7	26.392894668669364	32.82399858306855	22.787573107800878	17.995533640461208
8	24.284666529006778	37.19495791666635	20.758480769342285	17.761894784984584
9	25.12400738623643	17.35086085355203	21.34289775649624	36.18223400371531
10-11	24.036922619999082	27.25997788379259	28.535655955315782	20.167443540892542
12-13	24.900247634962565	24.631157026585335	28.935894513859438	21.532700824592656
14-15	21.926899933280662	26.11626309922524	26.8440995615181	25.112737405976
16-17	21.358539325665642	29.268361248071656	27.49585494105838	21.877244485204322
18-19	21.308915330820636	28.28555471003396	29.495170242407813	20.910359716737588
20-21	22.167603471356493	27.35432925544796	29.527078670793337	20.950988602402205
22-23	21.842508948892426	27.23480248454096	29.38455385078949	21.538134715777126
24-25	21.63610160533637	27.390759876280868	29.46560050678972	21.50753801159304
26-27	21.56539608663908	27.528657177009467	29.369935672092666	21.53601106425878
28-29	21.49034369326125	27.639807133332784	29.389637371935358	21.48021180147061
30-31	21.784896993281453	27.5042463642545	29.28005454805977	21.430802094404275
32-33	21.621222394128708	27.650277587748217	29.19729630377499	21.531203714348084
34-35	21.69608489865504	27.66896421240227	29.231874276507053	21.403076612435637
36-37	21.664753872205786	27.615419517667316	29.21230068223888	21.50752592788802
38-39	21.543603372603133	27.77108671514647	29.20061893254896	21.484690979701433
40-41	21.811973338219097	27.648402646019804	29.009621778887457	21.530002236873642
42-43	21.735874813612657	27.734501648278435	28.81358592967406	21.716037608434846
44-45	21.831281730810616	27.865285085254975	28.618916570199026	21.684516613735376
46-47	21.861760944780915	27.86365417817195	28.356799544091967	21.91778533295517
48-49	21.78774777390302	28.03023017070637	28.286922557931575	21.895099497459036
50-51	21.84175306961421	28.11614550515965	28.121359886450126	21.920741538776014
52-53	22.01159240823015	28.04948438704116	27.985673213967367	21.95324999076132
54-55	21.97038444215652	27.92674741943858	28.06494517946562	22.03792295893928
56-57	21.932262218914843	28.089987066444426	28.000674137557013	21.977076577083718
58-59	21.939648466265858	28.120339741552968	27.99696665751434	21.943045134666832
60-61	21.994570894421926	28.061820185245534	28.044968399939368	21.89864052039317
62-63	21.939884712795227	28.139797189182264	28.00544113559218	21.914876962430334
64-65	21.89348852633661	28.08935669178476	28.01852815583014	21.998626626048488
66-67	21.972997822294424	28.133580322821565	27.84754575267413	22.045876102209878
68-69	21.964604821001878	28.1202480176261	27.867411926443385	22.047735234928638
70-71	21.916688630060236	27.91940772727458	27.923858587163252	22.24004505550193
72-73	21.99183234769521	27.910768075714852	27.930821792968153	22.166577783621786
74-75	21.836609493452084	28.001382832582006	27.918785896084618	22.243221777881296
76-77	21.991480214694825	27.98003761930757	27.860464043141462	22.168018122856136
78-79	21.983644874956536	27.98874889910471	27.783070080624046	22.24453614531471
80-81	21.980291956967886	27.96179940539266	27.76922226130585	22.2886863763336
82-83	22.004824437666045	27.943199555187803	27.8656296224871	22.186346384659053
84-85	22.13463601348685	27.925568988719824	27.862230453021965	22.07756454477136
86-87	21.94513907469758	27.94080468653758	27.92158084332254	22.1924753954423
88-89	22.029573226322924	27.856516356650374	27.942988613877805	22.1709218031489
90-91	22.02290741819497	27.84991663820983	27.952546049856373	22.174629893738825
92-93	22.061849456024376	27.770936025795113	28.03900382389632	22.128210694284192
94-95	21.9102244854799	27.871914740955056	28.011821993413477	22.206038780151566
96-97	21.90294434213413	27.896898115215023	28.08414294619894	22.1160145964519
98-99	22.09260349821233	28.412845779944305	28.100230950754256	21.3943197710891
100-101	19.920551593085897	32.19023600235788	26.28081574631035	21.608396658245884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1844.0
1	1597.5
2	1967.0
3	2779.5
4	3735.5
5	5598.5
6	7397.0
7	9612.5
8	11559.0
9	11879.0
10	10750.0
11	9681.5
12	10942.0
13	15252.0
14	25143.0
15	38386.0
16	48859.0
17	53951.5
18	53692.0
19	50153.5
20	45010.5
21	39567.5
22	36382.5
23	37902.5
24	44224.0
25	55176.0
26	69296.0
27	84595.5
28	101641.0
29	119346.5
30	136140.0
31	154101.0
32	173866.0
33	193194.0
34	211716.5
35	228885.0
36	247722.0
37	264268.5
38	275148.5
39	285315.5
40	296662.5
41	307538.0
42	312948.5
43	316913.5
44	322805.0
45	326205.5
46	326259.0
47	324796.0
48	321529.0
49	316885.5
50	309934.5
51	297638.5
52	282291.5
53	266379.5
54	249572.0
55	230962.5
56	211404.0
57	190989.0
58	168656.5
59	145741.0
60	122178.5
61	98807.0
62	77314.5
63	58610.0
64	43136.0
65	31817.0
66	23628.5
67	17403.0
68	12926.5
69	9078.5
70	6133.0
71	4156.0
72	2783.5
73	1822.0
74	1147.0
75	722.5
76	471.5
77	313.5
78	210.0
79	145.0
80	102.0
81	68.5
82	47.0
83	31.0
84	19.5
85	12.5
86	9.0
87	7.0
88	5.5
89	3.5
90	4.0
91	4.0
92	3.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2606135784470374E-5
2	0.02946053932830727
3	1.2606135784470374E-5
4	1.2606135784470374E-5
5	1.2606135784470374E-5
6	1.2606135784470374E-5
7	1.2606135784470375E-4
8	6.429129250079891E-4
9	6.681251965769299E-4
10-11	1.2606135784470374E-5
12-13	1.2606135784470374E-5
14-15	0.002048497064976436
16-17	9.454601838352781E-5
18-19	6.303067892235187E-6
20-21	1.1345772502133635E-4
22-23	0.00194780586288304
24-25	6.998067398576252E-4
26-27	0.014592620573577013
28-29	0.3137844151742734
30-31	0.3144621720616219
32-33	0.33166896750690567
34-35	0.21531810599999787
36-37	0.07492807796137545
38-39	0.066378127363272
40-41	0.02684043323783697
42-43	0.011175828868028639
44-45	0.014754133740728799
46-47	0.011059697274626478
48-49	0.007757354742032967
50-51	0.008123292361659458
52-53	0.0039119023350943655
54-55	0.0021190510112813234
56-57	0.004304134983164791
58-59	0.005077450137744859
60-61	0.004336514652539204
62-63	0.002564651527446725
64-65	0.0011542609578666424
66-67	0.008027894791724699
68-69	0.011803341628339434
70-71	0.012056594731893475
72-73	0.010388991425560382
74-75	0.010486342815049478
76-77	0.009937343221798664
78-79	0.0024794493964349434
80-81	0.002078045291644142
82-83	0.0016070435565703938
84-85	0.0
86-87	0.002699111662720262
88-89	0.0
90-91	0.009327615502352352
92-93	0.019586046265417392
94-95	0.0014297128376312318
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	377.0
22-23	831.0
24-25	2096.0
26-27	2793.0
28-29	5032.0
30-31	14326.0
32-33	19038.0
34-35	27976.0
36-37	39825.0
38-39	47429.0
40-41	50303.0
42-43	59789.0
44-45	72019.0
46-47	71795.0
48-49	63129.0
50-51	55405.0
52-53	48815.0
54-55	53913.0
56-57	55501.0
58-59	56120.0
60-61	56581.0
62-63	54418.0
64-65	59069.0
66-67	61804.0
68-69	62713.0
70-71	61854.0
72-73	58651.0
74-75	58960.0
76-77	61387.0
78-79	65189.0
80-81	66588.0
82-83	64501.0
84-85	65007.0
86-87	69314.0
88-89	76174.0
90-91	83494.0
92-93	86162.0
94-95	98221.0
96-97	156429.0
98-99	705753.0
100-101	5113763.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.90588241502785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.23893314057784	77.5205517963204
2	6.9666738239464605	11.969565246875941
3	1.5602876044058527	4.021136504331439
4	0.5599687081188006	1.9241842398299493
5	0.2612983635234649	1.1223533246042992
6	0.13614432626251444	0.7017359090028452
7	0.07634284367815641	0.45908095465711896
8	0.05445807445677348	0.3742615152665932
9	0.03582631152282563	0.27699218145396143
>10	0.10770866525057396	1.4163759842438566
>50	0.00182954707935132	0.09989877643965386
>100	4.6003719948435254E-4	0.07189340517099885
>500	6.855397793099613E-5	0.04197016180294038
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2606135784470376E-5
2	0.0	1.2606135784470376E-5	0.0	0.0	1.2606135784470376E-5
3	0.0	1.2606135784470376E-5	0.0	2.521227156894075E-5	1.2606135784470376E-5
4	0.0	7.563681470682225E-5	0.0	2.521227156894075E-5	1.2606135784470376E-5
5	0.0	1.1345522206023338E-4	0.0	2.521227156894075E-5	1.2606135784470376E-5
6	8.824295049129263E-5	1.1345522206023338E-4	0.0	2.521227156894075E-5	1.2606135784470376E-5
7	8.824295049129263E-5	1.1345522206023338E-4	0.0	2.521227156894075E-5	1.2606135784470376E-5
8	1.00849086275763E-4	1.1345522206023338E-4	0.0	6.303067892235188E-5	1.2606135784470376E-5
9	1.00849086275763E-4	2.2691044412046676E-4	0.0	7.563681470682225E-5	3.7818407353411124E-5
10-11	1.00849086275763E-4	2.2691044412046676E-4	0.0	8.824295049129262E-5	3.7818407353411124E-5
12-13	1.00849086275763E-4	2.3321351201270195E-4	0.0	1.00849086275763E-4	3.7818407353411124E-5
14-15	1.00849086275763E-4	3.5297180196517053E-4	0.0	1.1345522206023338E-4	5.04245431378815E-5
16-17	2.01698172551526E-4	3.5297180196517053E-4	0.0	1.2606135784470375E-4	6.303067892235188E-5
18-19	2.01698172551526E-4	3.592748698574057E-4	0.0	1.7648590098258524E-4	6.933374681458707E-5
20-21	2.01698172551526E-4	3.6557793774964086E-4	0.0	2.4581964779717233E-4	9.454601838352781E-5
22-23	2.1430430833599637E-4	4.475178203486983E-4	0.0	6.113975855468131E-4	1.071521541679982E-4
24-25	2.1430430833599637E-4	4.7903315980987423E-4	0.0	0.001481220954675269	1.1345522206023338E-4
26-27	2.1430430833599637E-4	5.04245431378815E-4	0.0	0.004103297197845107	1.1345522206023338E-4
28-29	2.1430430833599637E-4	5.105484992710502E-4	0.0	0.01897853742352015	1.1345522206023338E-4
30-31	2.1430430833599637E-4	5.294577029477558E-4	0.0	0.02618294402434497	1.1345522206023338E-4
32-33	2.3321351201270195E-4	5.420638387322261E-4	0.0	0.029214719680510093	1.1345522206023338E-4
34-35	2.647288514738779E-4	6.050945176545781E-4	0.0	0.03288310519379097	1.1345522206023338E-4
36-37	2.647288514738779E-4	6.681251965769299E-4	0.0	0.03854956322891041	1.1345522206023338E-4
38-39	2.647288514738779E-4	7.185497397148114E-4	0.0	0.046857006710876385	1.1345522206023338E-4
40-41	2.647288514738779E-4	7.815804186371633E-4	0.0	0.05970896214314393	1.1345522206023338E-4
42-43	2.7733498725834827E-4	8.193988259905744E-4	0.0	0.06724112827436499	1.1345522206023338E-4
44-45	2.899411230428186E-4	9.328540480508078E-4	0.0	0.07711173259360529	1.1975828995246856E-4
46-47	3.088503267195242E-4	0.00100849086275763	0.0	0.08990696041484272	1.2606135784470375E-4
48-49	3.151533946117594E-4	0.0010210969985421003	0.0	0.10120205807772817	1.3866749362917414E-4
50-51	3.529718019651705E-4	0.0011282491527100985	0.0	0.11429353008990066	1.3866749362917414E-4
52-53	3.7818407353411125E-4	0.0011345522206023338	0.0	0.1272148192689828	1.449705615214093E-4
54-55	3.844871414263464E-4	0.0011471583563868043	0.0	0.17567910829237915	1.575766973058797E-4
56-57	3.9079020931858164E-4	0.0011849767637402153	0.0	0.19869160916692982	1.6387976519811488E-4
58-59	3.9079020931858164E-4	0.0011975828995246856	0.0	0.22425685253783573	1.6387976519811488E-4
60-61	4.03396345103052E-4	0.0012669166463392728	0.0	0.23862154426423973	1.6387976519811488E-4
62-63	4.916392955943447E-4	0.0012984319858004487	0.0	0.24677771411679206	1.8909203676705563E-4
64-65	4.916392955943447E-4	0.0014370994794296228	0.0	0.2545872152352715	1.8909203676705563E-4
66-67	4.916392955943447E-4	0.0014623117509985637	0.0	0.2601654303198996	1.8909203676705563E-4
68-69	4.916392955943447E-4	0.0015001301583519747	0.0	0.2651700662263343	1.8909203676705563E-4
70-71	4.979423634865798E-4	0.0015253424299209153	0.0	0.2697902149913427	2.1430430833599637E-4
72-73	5.168515671632853E-4	0.0015253424299209153	0.0	0.27375484469555866	2.3951657990493714E-4
74-75	5.168515671632853E-4	0.001544251633597621	0.0	0.2767866203517238	2.3951657990493714E-4
76-77	5.357607708399909E-4	0.0015946761767355025	0.0	0.27981209293999665	2.3951657990493714E-4
78-79	5.420638387322261E-4	0.0016009792446277378	0.0	0.2824782906584122	2.3951657990493714E-4
80-81	5.420638387322261E-4	0.0016009792446277378	0.0	0.28372629810107475	2.3951657990493714E-4
82-83	5.420638387322261E-4	0.0016072823125199728	0.0	0.2840855729709322	2.647288514738779E-4
84-85	5.420638387322261E-4	0.0016577068556578544	0.0	0.28435660489029824	2.647288514738779E-4
86-87	5.483669066244613E-4	0.0016892221951190303	0.0	0.284482666248143	2.8363805515058344E-4
88-89	5.546699745166965E-4	0.0017774651456103228	0.0	0.2845204846554964	2.8994112304281866E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10885	0.0	54.09164	1
GTATCAA	20725	0.0	37.761604	1
TCAACGC	25605	0.0	29.935701	4
ATCAACG	25740	0.0	29.845615	3
CAACGCA	26260	0.0	29.205418	5
TATCAAC	26565	0.0	29.168688	2
AACGCAG	27060	0.0	28.37382	6
ACGCAGA	31105	0.0	24.683992	7
CGCAGAG	31350	0.0	24.491085	8
TGGTATC	4705	0.0	22.703226	2
GCAGAGT	36190	0.0	21.216618	9
GTGGTAT	5360	0.0	20.73218	1
GAGTACT	22010	0.0	20.172142	12-13
TACATGG	23835	0.0	20.004515	2
GTACATG	24650	0.0	19.902006	1
AGAGTAC	33015	0.0	19.3243	10-11
ACATGGG	24535	0.0	18.955355	3
CAGAGTA	35375	0.0	18.94203	10-11
GTACTTT	24250	0.0	18.220428	14-15
AGTACTT	24135	0.0	17.602047	12-13
>>END_MODULE