FastQCFastQC Report
Fri 27 May 2016
SRR2140402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8102512
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156600.057.5398671
GTATCAA298550.039.4495161
TCAACGC352550.033.002424
ATCAACG352350.032.9863473
TATCAAC358400.032.537582
CAACGCA362500.032.1677785
GTGGTAT58000.031.8282851
AACGCAG372000.031.364536
TGGTATC56750.030.406222
ACGCAGA417750.027.939937
CGCAGAG421100.027.7074438
GCAGAGT477350.024.48759
GAGTACT289250.022.85243412-13
CAGAGTA470450.021.86651810-11
GTACATG319950.020.8196181
GTACTTT315900.020.78218314-15
TACATGG313700.020.562272
ACATGGG316150.019.6270943
AGAGTAC443600.019.24424610-11
ACTTTTT357900.018.4164916-17
Produced by FastQC (version 0.11.5)