##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8102512
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.646894938261124	32.0	18.0	33.0	18.0	33.0
2	29.764272240510103	31.0	28.0	33.0	25.0	33.0
3	31.013848174492058	33.0	31.0	33.0	27.0	33.0
4	30.841726368316394	33.0	31.0	33.0	27.0	34.0
5	31.268223237435503	33.0	32.0	33.0	28.0	34.0
6	34.114467834172906	37.0	33.0	38.0	26.0	38.0
7	35.563674820845684	38.0	36.0	38.0	30.0	38.0
8	35.34299214860774	38.0	35.0	38.0	29.0	38.0
9	36.03647189908512	38.0	37.0	38.0	31.0	38.0
10-11	36.26292148657108	38.0	37.0	38.0	33.0	38.0
12-13	36.364109488514174	38.0	37.0	38.0	33.0	38.0
14-15	36.35119441970589	38.0	37.0	38.0	33.0	38.0
16-17	36.381597275017924	38.0	37.0	38.0	33.0	38.0
18-19	36.34832660537867	38.0	37.0	38.0	33.0	38.0
20-21	36.39749891637467	38.0	37.0	38.0	33.5	38.0
22-23	36.44528737825319	38.0	37.5	38.0	34.0	38.0
24-25	36.44818719257576	38.0	38.0	38.0	34.0	38.0
26-27	36.39899290295471	38.0	38.0	38.0	33.5	38.0
28-29	36.37665731402714	38.0	37.5	38.0	33.0	38.0
30-31	36.37002449067795	38.0	37.0	38.0	33.0	38.0
32-33	36.347263728188466	38.0	37.0	38.0	33.0	38.0
34-35	36.32138841858203	38.0	37.0	38.0	33.0	38.0
36-37	36.279207173247215	38.0	37.0	38.0	33.0	38.0
38-39	36.27101106587815	38.0	37.0	38.0	33.0	38.0
40-41	36.25214817535078	38.0	37.0	38.0	33.0	38.0
42-43	36.20729465571044	38.0	37.0	38.0	33.0	38.0
44-45	36.14077829436502	38.0	37.0	38.0	33.0	38.0
46-47	36.07754446504127	38.0	37.0	38.0	33.0	38.0
48-49	36.019817429073306	38.0	37.0	38.0	31.5	38.0
50-51	35.98864973911501	38.0	37.0	38.0	31.0	38.0
52-53	35.955499771550635	38.0	37.0	38.0	31.0	38.0
54-55	35.86796505065972	38.0	37.0	38.0	31.0	38.0
56-57	35.89261442844979	38.0	37.0	38.0	31.0	38.0
58-59	35.871207550812734	38.0	37.0	38.0	31.0	38.0
60-61	35.8659453173162	38.0	37.0	38.0	31.0	38.0
62-63	35.84943866509893	38.0	37.0	38.0	31.0	38.0
64-65	35.852462799378394	38.0	37.0	38.0	31.0	38.0
66-67	35.8448262221528	38.0	37.0	38.0	31.0	38.0
68-69	35.822452556318424	38.0	37.0	38.0	31.0	38.0
70-71	35.80286662116868	38.0	37.0	38.0	31.0	38.0
72-73	35.79244306182281	38.0	37.0	38.0	31.0	38.0
74-75	35.781124508043206	38.0	37.0	38.0	31.0	38.0
76-77	35.789996506888215	38.0	37.0	38.0	31.0	38.0
78-79	35.75565923165915	38.0	37.0	38.0	31.0	38.0
80-81	35.74175276085306	38.0	37.0	38.0	31.0	38.0
82-83	35.65348717929046	38.0	36.5	38.0	29.5	38.0
84-85	35.67418165620373	38.0	36.0	38.0	29.0	38.0
86-87	35.673146396790344	38.0	36.0	38.0	29.0	38.0
88-89	35.68085438988034	38.0	36.0	38.0	29.5	38.0
90-91	35.659683128024774	38.0	36.0	38.0	29.0	38.0
92-93	35.62465730792241	38.0	36.0	38.0	29.0	38.0
94-95	35.63651443860098	38.0	36.0	38.0	29.0	38.0
96-97	35.65875454596789	38.0	36.0	38.0	30.5	38.0
98-99	35.66379436950439	38.0	36.0	38.0	31.0	38.0
100-101	35.06683751768597	38.0	35.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	21.0
20	118.0
21	746.0
22	3106.0
23	8659.0
24	19272.0
25	35264.0
26	56886.0
27	83981.0
28	116388.0
29	153384.0
30	194430.0
31	243630.0
32	302634.0
33	386488.0
34	518406.0
35	773421.0
36	1532985.0
37	3672687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.821010570548985	18.448513251199135	13.85809734067657	20.872378837575308
2	14.964976293771612	20.32647406137751	43.24797050593692	21.460579138913957
3	18.50386178776496	27.314807843690964	29.796613416148638	24.384716952395433
4	12.477908085788703	19.510813436623113	38.41534575943855	29.59593271814963
5	14.158023265103568	37.50814187250658	34.75186412608552	13.581970736304335
6	29.147540910769397	38.323818588605604	18.86063235697769	13.668008143647302
7	26.174191411256164	33.95411200872026	22.398461119218336	17.473235460805242
8	24.119013955178346	36.96285793837763	20.736038403892522	18.182089702551504
9	25.493365514299764	17.730489013777458	21.30419553837131	35.47194993355147
10-11	23.943142571094	27.418422829858198	28.2961321131027	20.342302485945098
12-13	25.131557966220846	25.04153650127269	28.780290606172503	21.046614926333955
14-15	22.158794704654554	26.24948287642154	27.068790518298524	24.522931900625387
16-17	21.038553084241187	29.648589476079835	27.633940546893932	21.678916892785054
18-19	21.053310380780676	28.219402822235867	30.128551491191867	20.59873530579159
20-21	22.09675862649473	27.084548522358944	30.318171147818905	20.500521703327422
22-23	21.743697057619908	27.09987044223491	30.185120167558004	20.971312332587182
24-25	21.348156970419016	27.251379539400922	30.317295605286994	21.08316788489307
26-27	21.273221647462186	27.38225906355224	30.24495626616609	21.09956302281948
28-29	21.184279291273832	27.420806670677838	30.30135247676945	21.09356156127888
30-31	21.455036129820744	27.25675182479495	30.223618156640182	21.06459388874412
32-33	21.23072841044117	27.396224277902505	30.179689711359288	21.193357600297034
34-35	21.415108547672553	27.467715535859227	30.11819536046118	20.99898055600704
36-37	21.366935711498776	27.37343358239655	30.084456103504344	21.175174602600332
38-39	21.20391226877746	27.50047981992526	30.160059394146327	21.13554851715095
40-41	21.501619334342482	27.40585170369026	29.962632591661727	21.129896370305527
42-43	21.38897472579876	27.466444126529964	29.810376728659993	21.334204419011286
44-45	21.459460412569108	27.602517925068167	29.58670956179961	21.35131210056312
46-47	21.565805553238786	27.6331040852811	29.228064686078152	21.573025675401958
48-49	21.518072571166332	27.785061711258486	29.135327100628405	21.561538616946777
50-51	21.522399504488185	27.935949386140614	28.967581927009885	21.574069182361317
52-53	21.674863588262546	27.89869999213792	28.819586800186002	21.60684961941353
54-55	21.66003564741717	27.81368575982593	28.822339547805715	21.70393904495119
56-57	21.62410011648288	27.88146120613047	28.831953689492078	21.662484987894572
58-59	21.60557662160899	27.95819436260814	28.820922631536604	21.615306384246264
60-61	21.66267952500156	27.897179635959134	28.87333221930423	21.566808619735074
62-63	21.582531964942586	27.979802919461992	28.83902565981311	21.598639455782312
64-65	21.563961095091937	27.962371767587936	28.773119293350003	21.700547843970128
66-67	21.592268045038455	28.01144538351622	28.64250770207026	21.753778869375065
68-69	21.573361865373837	27.970363222025856	28.727702017509433	21.72857289509087
70-71	21.565636923436386	27.82142851774056	28.74720312301019	21.86573143581287
72-73	21.603317246970022	27.82280122403008	28.70326115594908	21.870620373050816
74-75	21.483590023583655	27.89739010676518	28.71159635611259	21.90742351353858
76-77	21.663267838548453	27.849635490772233	28.599180866231606	21.88791580444771
78-79	21.67119093554467	27.879558754070022	28.486012479043136	21.963237831342177
80-81	21.614086444778184	27.934259111395477	28.505916073051306	21.945738370775032
82-83	21.649875499956693	27.947870526634844	28.545985388827038	21.856268584581425
84-85	21.81365394334255	27.92704199219132	28.47270960344785	21.786594461018282
86-87	21.648232392453266	27.964075840374488	28.534388256262684	21.853303510909566
88-89	21.730203547463667	28.013142570284266	28.449396234699183	21.807257647552884
90-91	21.71905466319785	27.994875491014966	28.47230664121571	21.81376320457148
92-93	21.684183820206748	27.95567658107346	28.546289015123193	21.813850583596597
94-95	21.632153681019307	28.07123294692067	28.505553877788415	21.791059494271607
96-97	21.64100772501495	28.101619191082634	28.514581762059677	21.74279132184274
98-99	21.860722894716268	28.677172836369568	28.559605101438034	20.90249916747613
100-101	22.487468168859927	31.122874998709083	26.017243981561272	20.37241285086972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3112.0
1	2613.0
2	2984.0
3	4003.5
4	4764.5
5	6913.5
6	9204.5
7	11992.5
8	15916.5
9	17838.0
10	16312.0
11	14475.5
12	15233.0
13	18929.5
14	27254.0
15	37976.0
16	47387.0
17	54490.0
18	56339.5
19	52507.5
20	46216.0
21	39732.0
22	36091.0
23	37577.0
24	43584.5
25	54204.0
26	68770.5
27	84713.0
28	102975.0
29	123313.0
30	142437.5
31	161833.5
32	182591.5
33	203193.0
34	222923.5
35	240823.0
36	259653.5
37	276853.0
38	288807.5
39	299044.0
40	309918.0
41	319448.0
42	325624.5
43	331118.0
44	336708.0
45	339756.5
46	339258.5
47	336643.0
48	331827.5
49	324597.0
50	315545.5
51	302409.5
52	285281.0
53	267322.5
54	248818.0
55	227480.5
56	205839.5
57	184419.5
58	162782.5
59	140638.5
60	116246.0
61	92780.5
62	72087.5
63	54714.5
64	40869.0
65	30056.0
66	21673.0
67	16030.5
68	12037.0
69	8687.5
70	6127.0
71	4266.5
72	2856.0
73	1909.5
74	1274.5
75	775.5
76	481.0
77	302.0
78	200.5
79	122.0
80	76.0
81	49.5
82	34.0
83	23.5
84	16.5
85	11.0
86	7.0
87	7.0
88	5.0
89	3.5
90	3.5
91	2.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.00377660656349537
4	0.0
5	0.04868860422545502
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.049057378748714E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.531190439854223E-4
28-29	1.8530175371432733E-4
30-31	6.183753831685765E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.4508007531009204E-5
44-45	0.0
46-47	6.928679091363958E-4
48-49	0.0012749354490374956
50-51	0.002288609636009498
52-53	0.005077196985872832
54-55	0.006364249061775176
56-57	0.00530604091072158
58-59	0.007378311866703391
60-61	0.006707019310186833
62-63	0.0053527603770857374
64-65	0.005597675432993074
66-67	0.0010605268669381521
68-69	0.002055920761127388
70-71	4.4381893018019547E-4
72-73	0.00152473910882829
74-75	0.0020707454129343422
76-77	4.8575479892621693E-4
78-79	0.0
80-81	1.6525106594448947E-4
82-83	0.0
84-85	0.0
86-87	9.294648489948805E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	6.325376388278201E-4
96-97	2.466085569223515E-5
98-99	5.602474392235513E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	109.0
20-21	420.0
22-23	978.0
24-25	1471.0
26-27	3195.0
28-29	6835.0
30-31	15894.0
32-33	19986.0
34-35	27882.0
36-37	43384.0
38-39	50884.0
40-41	53327.0
42-43	61835.0
44-45	75287.0
46-47	75859.0
48-49	68784.0
50-51	59791.0
52-53	51848.0
54-55	54483.0
56-57	55832.0
58-59	60627.0
60-61	60445.0
62-63	56980.0
64-65	61145.0
66-67	65844.0
68-69	67110.0
70-71	67439.0
72-73	61989.0
74-75	63064.0
76-77	66353.0
78-79	69195.0
80-81	70278.0
82-83	66859.0
84-85	65130.0
86-87	67942.0
88-89	71908.0
90-91	74356.0
92-93	73563.0
94-95	77851.0
96-97	125305.0
98-99	671291.0
100-101	5309754.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.6216706680829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.40088500220361	78.30675688764114
2	6.913368047306603	11.9769498040208
3	1.5786133442947448	4.102263756652209
4	0.5331700134818982	1.847363092717052
5	0.23666324149724968	1.0250082682107866
6	0.12274343028455537	0.6379344596867728
7	0.0644311664825737	0.39067946986697444
8	0.0409869332538261	0.2840285307206096
9	0.02565291383680765	0.19998884286437932
>10	0.08191716060348297	1.0553781850570958
>50	0.0011140730269762595	0.0642836074356312
>100	3.9117046898223535E-4	0.05670466534966365
>500	2.1168080545234078E-5	0.01050661109453884
>1k	4.2335178170382616E-5	0.04215381868230545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.2341851514690753E-5	0.0	0.0	1.2341851514690753E-5
2	0.0	1.2341851514690753E-5	0.0	2.4683703029381507E-5	1.2341851514690753E-5
3	0.0	1.2341851514690753E-5	0.0	3.7025554544072255E-5	1.2341851514690753E-5
4	0.0	2.4683703029381507E-5	0.0	8.639296060283527E-5	1.2341851514690753E-5
5	0.0	2.4683703029381507E-5	0.0	9.873481211752603E-5	1.2341851514690753E-5
6	4.936740605876301E-5	2.4683703029381507E-5	0.0	9.873481211752603E-5	2.4683703029381507E-5
7	4.936740605876301E-5	2.4683703029381507E-5	0.0	9.873481211752603E-5	2.4683703029381507E-5
8	4.936740605876301E-5	2.4683703029381507E-5	0.0	9.873481211752603E-5	3.7025554544072255E-5
9	4.936740605876301E-5	1.1107666363221677E-4	0.0	1.1107666363221677E-4	3.7025554544072255E-5
10-11	6.170925757345376E-5	1.1107666363221677E-4	0.0	1.9129869847770667E-4	3.7025554544072255E-5
12-13	6.170925757345376E-5	1.1107666363221677E-4	0.0	2.344951787791243E-4	4.3196480301417634E-5
14-15	6.170925757345376E-5	1.357603666615983E-4	0.0	3.085462878672688E-4	6.788018333079914E-5
16-17	9.256388636018065E-5	1.357603666615983E-4	0.0	3.1471721362461415E-4	7.405110908814451E-5
18-19	1.1107666363221677E-4	1.4193129241894364E-4	0.0	3.5791369392603183E-4	9.873481211752603E-5
20-21	1.357603666615983E-4	1.4810221817628902E-4	0.0	3.8259739695541335E-4	9.873481211752603E-5
22-23	1.4810221817628902E-4	1.7895684696301591E-4	0.0	4.25793877256831E-4	9.873481211752603E-5
24-25	1.542731439336344E-4	2.0364054999239743E-4	1.2341851514690753E-5	5.183577636170115E-4	9.873481211752603E-5
26-27	1.6044406969097978E-4	2.0981147574974278E-4	1.2341851514690753E-5	6.356053530065738E-4	1.1107666363221677E-4
28-29	1.6044406969097978E-4	2.0981147574974278E-4	1.2341851514690753E-5	0.0012033305226823484	1.1107666363221677E-4
30-31	1.6044406969097978E-4	2.2215332726443354E-4	1.2341851514690753E-5	0.002844796774136219	1.1107666363221677E-4
32-33	1.7278592120567054E-4	2.2215332726443354E-4	1.2341851514690753E-5	0.005788328360389963	1.6044406969097978E-4
34-35	2.0364054999239743E-4	2.5917888180850576E-4	1.2341851514690753E-5	0.009651327884488168	1.7278592120567054E-4
36-37	2.1598240150708816E-4	2.9620443635257804E-4	1.2341851514690753E-5	0.0158160827160762	1.7278592120567054E-4
38-39	2.2215332726443354E-4	3.2088813938195955E-4	1.2341851514690753E-5	0.028022173864105354	1.851277727203613E-4
40-41	2.344951787791243E-4	3.702555454407226E-4	1.2341851514690753E-5	0.06127112184468224	1.851277727203613E-4
42-43	2.715207333231966E-4	3.9493924847010405E-4	1.2341851514690753E-5	0.0732612305912043	1.9129869847770667E-4
44-45	2.7769165908054193E-4	4.8133220907293936E-4	1.2341851514690753E-5	0.08881196349971465	1.9746962423505205E-4
46-47	2.8386258483788733E-4	5.430414666463932E-4	1.2341851514690753E-5	0.10613992302634047	2.2832425302177892E-4
48-49	2.9620443635257804E-4	5.492123924037385E-4	1.2341851514690753E-5	0.12222135554998252	2.715207333231966E-4
50-51	2.9620443635257804E-4	6.788018333079913E-4	1.2341851514690753E-5	0.13788933604788245	2.715207333231966E-4
52-53	2.9620443635257804E-4	6.911436848226821E-4	1.2341851514690753E-5	0.16283221795907243	2.7769165908054193E-4
54-55	2.9620443635257804E-4	6.911436848226821E-4	1.2341851514690753E-5	0.21870377976607747	2.8386258483788733E-4
56-57	2.9620443635257804E-4	7.158273878520637E-4	1.2341851514690753E-5	0.28141889823797855	2.8386258483788733E-4
58-59	3.1471721362461415E-4	7.21998313609409E-4	1.2341851514690753E-5	0.3516687170595983	2.8386258483788733E-4
60-61	3.2088813938195955E-4	7.405110908814451E-4	1.2341851514690753E-5	0.374908423461761	2.9620443635257804E-4
62-63	3.4557184241134107E-4	7.651947939108267E-4	1.2341851514690753E-5	0.386701062584048	2.9620443635257804E-4
64-65	3.4557184241134107E-4	8.392459029989711E-4	1.2341851514690753E-5	0.3973027130351674	2.9620443635257804E-4
66-67	3.4557184241134107E-4	8.51587754513662E-4	1.2341851514690753E-5	0.40365259563947575	2.9620443635257804E-4
68-69	3.5791369392603183E-4	8.639296060283528E-4	1.2341851514690753E-5	0.4093236764104762	3.0237536210992344E-4
70-71	3.5791369392603183E-4	8.762714575430435E-4	1.2341851514690753E-5	0.4149762444042045	3.085462878672688E-4
72-73	3.702555454407226E-4	8.762714575430435E-4	1.2341851514690753E-5	0.41897500429496437	3.5791369392603183E-4
74-75	3.887683227127587E-4	9.00955160572425E-4	1.2341851514690753E-5	0.4224492354963498	3.5791369392603183E-4
76-77	4.011101742274495E-4	9.379807151164972E-4	1.2341851514690753E-5	0.42546681819169163	3.702555454407226E-4
78-79	4.0728109998479486E-4	9.503225666311879E-4	1.2341851514690753E-5	0.42819436737643835	3.702555454407226E-4
80-81	4.0728109998479486E-4	9.503225666311879E-4	1.2341851514690753E-5	0.4293298177157899	3.887683227127587E-4
82-83	4.0728109998479486E-4	9.503225666311879E-4	1.2341851514690753E-5	0.4298358336278922	4.1962295149948557E-4
84-85	4.1962295149948557E-4	9.626644181458787E-4	1.2341851514690753E-5	0.4303665332430239	4.1962295149948557E-4
86-87	4.1962295149948557E-4	9.626644181458787E-4	1.2341851514690753E-5	0.4305763447187736	4.1962295149948557E-4
88-89	4.257938772568309E-4	0.0010243736757193326	1.2341851514690753E-5	0.4306442249021044	4.257938772568309E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15660	0.0	57.539867	1
GTATCAA	29855	0.0	39.449516	1
TCAACGC	35255	0.0	33.00242	4
ATCAACG	35235	0.0	32.986347	3
TATCAAC	35840	0.0	32.53758	2
CAACGCA	36250	0.0	32.167778	5
GTGGTAT	5800	0.0	31.828285	1
AACGCAG	37200	0.0	31.36453	6
TGGTATC	5675	0.0	30.40622	2
ACGCAGA	41775	0.0	27.93993	7
CGCAGAG	42110	0.0	27.707443	8
GCAGAGT	47735	0.0	24.4875	9
GAGTACT	28925	0.0	22.852434	12-13
CAGAGTA	47045	0.0	21.866518	10-11
GTACATG	31995	0.0	20.819618	1
GTACTTT	31590	0.0	20.782183	14-15
TACATGG	31370	0.0	20.56227	2
ACATGGG	31615	0.0	19.627094	3
AGAGTAC	44360	0.0	19.244246	10-11
ACTTTTT	35790	0.0	18.41649	16-17
>>END_MODULE