FastQCFastQC Report
Fri 27 May 2016
SRR2140401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8503873
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112000.050.1207351
GTATCAA208250.038.18551
TCAACGC257300.030.3924354
ATCAACG258650.030.200453
CAACGCA265450.029.4593095
TATCAAC268150.029.414342
AACGCAG273700.028.602856
ACGCAGA314250.024.912017
CGCAGAG315100.024.8858788
CTTATAC76750.023.4431421
GCAGAGT367650.021.2822659
TGGTATC53450.021.0694642
GTGGTAT59050.020.678771
GTACATG249100.019.625211
AGAGTAC333100.019.38484410-11
GAGTACT222700.019.30066312-13
TACATGG247450.019.267952
TATACAC116200.018.5225983
CAGAGTA358350.018.51245710-11
ACATGGG246350.018.4367053
Produced by FastQC (version 0.11.5)